LOCUS       sequence-483817-       14781 bp    DNA     circular SYN 19-AUG-2025
DEFINITION  Expresses mCherry for monosynaptic circuit mapping.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14781)
  AUTHORS   Reardon TR, Murray AJ, Turi GF, Wirblich C, Croce KR, Schnell MJ, 
            Jessell TM, Losonczy A
  TITLE     Rabies Virus CVS-N2c Strain Enhances Retrograde Synaptic Transfer 
            and Neuronal Viability.
  JOURNAL   Neuron. 2016 Jan 19. pii: S0896-6273(16)00005-2. doi: 
            10.1016/j.neuron.2016.01.004.
  PUBMED    26804990
REFERENCE   2  (bases 1 to 14781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 19, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2016 Jan 19. pii: S0896-6273(16)00005-2. doi: 
            10.1016/j.neuron.2016.01.004."
COMMENT     Sequence Label: RabV CVS-N2c(deltaG)-mCherry
FEATURES             Location/Qualifiers
     source          1..14781
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     553..572
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        553..571
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      3956..3965
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3962..4672
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4107..4125)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4375..4394
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             6847..6858
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(8650..8661)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(11857..11875)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      11871..11918
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(11946..11962)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11946..11962)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11959..11981)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11970..11986
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11994..12000,12001..12018,12019..12024))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12039..12060
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12177..12194)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12348..12936)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12428..12447)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13107..13898,13899..13967))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGASKGGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13730..13749
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13968..14072)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      14099..14555
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(14186..14205)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14396..14417
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     14681..14703
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     14695..14712
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     14696..14712
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      541 gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctaga ttaagcgtct
      601 gatgagtccg tgaggacgaa acccggcgta ccgggtcacg cttaacaaca aaaccagaga
      661 agaaaaagac agcgtcaatt gcaaagcaaa aatgtaacac ccctacaatg gatgccgaca
      721 agattgtgtt caaagtcaat aatcaggtgg tctctttgaa gcctgagatt atcgtggatc
      781 aatatgagta caagtaccct gccatcaagg atttgaaaaa gccttgtatc accctaggga
      841 aagcccccga cttgaacaaa gcatacaaat cagttttatc aggcatgaat gccgccaaac
      901 ttgatccgga tgatgtatgc tcctacttgg cagcagcaat gcagttcttt gaggggacat
      961 gtccggaaga ctggaccagc tatggaatcc tgattgcacg aaaaggagat aggatcaccc
     1021 caaactctct agtggagata aagcgtactg atgtagaagg gaattgggct ctgacaggag
     1081 gcatggaatt gacaagggac cccactgtct ctgaacatgc atctttagtc ggtcttctcc
     1141 tgagtctgta caggttgagc aaaatatcag gacagaacac tggtaactat aagacaaaca
     1201 ttgcagatag gatagagcag attttcgaga cagcaccttt tgttaagatc gtggaacacc
     1261 ataccctaat gacaactcac aagatgtgtg ctaattggag tactataccg aacttcagat
     1321 ttttggccgg aacctacgac atgtttttct cacggattga gcatctatat tcggcaatca
     1381 gagtgggcac agtcgtcacc gcttatgaag actgctcagg actggtatcg tttacagggt
     1441 tcataaagca gatcaatctc accgcaaggg aagcaatact atatttcttc cacaagaact
     1501 ttgaggaaga gataagaaga atgttcgagc cagggcaaga gacagctgtt cctcactctt
     1561 atttcatcca cttccgttca ctaggcttga gtgggaagtc tccttattca tcgaatgctg
     1621 tcggtcatgt gttcaatctc attcactttg ttggatgcta catgggtcaa gtcagatctc
     1681 taaatgcgac ggttattgct gcatgtgccc ctcatgagat gtctgttcta gggggctatt
     1741 tgggagagga attcttcgga aaagggacat ttgaaagaag gttcttcaga gacgagaaag
     1801 aacttcaaga atatgaggcg gctgaactaa caaagtccga cgtggcactg gcagatgacg
     1861 gaaccgtcaa ctctgatgac gaggactatt tctctggtga aaccagaagt ccagaagctg
     1921 tctatactcg aatcatgatg aatggaggtc gactgaagag atctcatata cggagatatg
     1981 tctcagtcag ttccaatcat caagcccgtc caaactcatt cgccgaattt ttaaacaaga
     2041 cgtattcgaa tgactcataa ggagttgatt gacagggtgc cagaaatcta tagattgtat
     2101 atatccatca tgaaaaaaac taacactcct cctttcaaac catcccaaat atgagcaaga
     2161 tctttgttaa tccgagtgca atcagagccg gtctggccga tcttgagatg gccgaagaga
     2221 ctgttgatct gatcaacaga aacatagaag acaatcaggc tcatctccag ggagaaccca
     2281 tagaagtgga caacctccct gaggacatga agcgacttca cctggacgat gaaaaatcgt
     2341 ccaaccttgg tgagatggtt agggtgggag aaggcaagta tcgagaggac tttcagatgg
     2401 atgagggaga ggaccccaac ctcctgttcc aatcgtacct ggataatgtt ggagtccaaa
     2461 tagtcagaca aatgaggtca ggagagagat tcctcaagat atggtcacag accgtagagg
     2521 aaattgtatc ctatgtcacg gtcaactttc ctaaccctcc aagaaggtct tcggaggata
     2581 aatcaaccca gactactggc agagagctca agaaggagac aacgtctgct ttctctcaga
     2641 gagaaagcca accttcgaaa gctaggatgg tggctcaagt tgcccctggt cctccagccc
     2701 ttgaatggtc agccaccaat gaagaagatg atctatcagt agaggctgag atcgctcatc
     2761 agattgctga aagcttttcc aagaagtaca agtttccctc ccgatcttca ggaatattct
     2821 tgtataattt tgagcaactg aagatgaacc ttgatgacat agttaaagag gcaaaaaatg
     2881 taccgggcgt gacccgtctg gcccatgatg gatccaaaat ccccctgaga tgcgtactgg
     2941 gatgggtcgc tttggctaat tccaaaaaat tccaattact agtcgaggct gacaagctaa
     3001 gcaaaatcat gcaagatgat ttgaatcgct atacatcctg ctaaccgagt tttcgaactc
     3061 agtccctcca gataatgaaa actgagatgt tatggagtag acatgaaaaa aacaggcaac
     3121 accactgata aaatgaacgt tctacgcaag atagtgaaaa aatgtaggga tgaggacact
     3181 caaaagccct ctcctgtgtc agcccctccg tatgacgatg acctgtggct tccacctcct
     3241 gaatatgtcc cgctgaaaga actcacaagc aagaagaaca tgaggaactt ttgtgtcaac
     3301 ggggaggtta aagcgtgtag cccaaatggt tactcattca ggattttgcg gcacattctg
     3361 agatcattca acgagatata ctctgggaat cataggatga ttgggttagt caaaattgtt
     3421 gttggactag ctttatcagg agctccagta cctgagggca tgaactgggt atacaaattg
     3481 aggagaaccc ttatattcca gtgggctgat tccaggggcc ctcttgaagg ggaggagtta
     3541 gattactctc aagagatcac ttgggatgat gatactgaat tcgtcggatt gcaaataaga
     3601 gtgagcgcaa gacaatgtca tattcaaggc aggatctggt gtatcaacac gaactcgagg
     3661 gcatgtcaac tatggtctga catgtctctt cagacacaaa ggtctgaaga ggacaaagac
     3721 tcttctctgc ttctagaata atcagattat atcctgcaag tgtatcactt gtttacctct
     3781 ggaggagaga gcatacaggc ttgactccga tccttgggag caatagaaca aaaaaacaca
     3841 cgttatggtg ccgttaaatc gctgcatttt atcaaagtca agttgataac ctttacattt
     3901 tgagcctctt ggatgtgaaa aaaactatta acatccctca aaagaccccg ggaaagccac
     3961 catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa
     4021 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggag agggcgaggg
     4081 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc
     4141 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca
     4201 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg
     4261 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
     4321 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt
     4381 aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg
     4441 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc
     4501 tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt
     4561 caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
     4621 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt aagctagcgg
     4681 cgcctagccg gtcatccttt tgacgattcc agtcccgagg ataacctcct ctcgggattg
     4741 gggggaatct ttggatccag tagtcctcct tgaactccgt ccaacagggc agattcaaga
     4801 gtcataagac tttcattaat catttcagtt gatcagacat ggtcgtgtag attctcttaa
     4861 tacgggagat cttctagcag tttcagtgac caacggtgct ttcattctcc aggaactgat
     4921 accaaaggtt gtggacaggc caaggggtac ttcggatgac tctgtgcttg ggcacagaaa
     4981 gaggtcgtag tgtgcccccc gatagcggac tcgacatgaa tcaactaaga aaggcaatct
     5041 gcctcccatg atggacataa gcaatagttc acaaccatct tgcatctcag tgaagtgtac
     5101 ataactattg agggctgggt catctaagca tttcagtcga gaaaaaaact gtagaccaaa
     5161 agaacaacta gcaacacttc tcatccagag acccatatca agatgctaga tccgggagag
     5221 gtttatgatg accctattga tccaattgag tcagaggctg aacccagagg aacccccact
     5281 gtccccaaca tcttgaggaa ctccgactac aatctcaatt ctcctttgat agaggatcct
     5341 gccaaactaa tgttagaatg gttgaagaca gggaacagac cttatcggat gactttgaca
     5401 gacaattgct ccaggtctta caaagttttg aaagattatt tcaagaaagt agatttgggt
     5461 tctctcaaag tgggcggaac tgctgcacag tcaatggttt ctctctggtt gtgtggtgcc
     5521 cactctgaat caaacaggag ccggagatgt ataaccgact tggcccattt ctattccaag
     5581 tcatccccca tagagaagct attgaattgt acgctaggaa acagaggcct gagaatccca
     5641 ccagaggggg tgttaaattg cctcgagagg gtcaattatg acaaggcatt tgggaggtat
     5701 ctggccaaca cgtattcctc ttacttgttt ttccatgtaa tcaccttata catgaatgcc
     5761 ttagactggg aagaggaaaa gaccatcctg gcattatgga aagatataac ctcagtggat
     5821 accgagaagg acttggtcaa attcaaagat caaatatggg gactgttgat tgtgacaaag
     5881 gactttgttt actctcagag ttcaaactgt ctttttgaca gaaactacac actgatgcta
     5941 aaggatcttt tcttgtctcg attcaactcc ttaatgattc tgctttctcc ccctgagccc
     6001 cgatactcag atgacttaat atctcagctg tgccagctat acatcgctgg ggatcaagtc
     6061 ttgtccatgt gtgggaactc cggctatgaa gtcatcaaaa tattggagcc atatgtcgtg
     6121 aacagtttgg tccagagggc agagaagttt aggcctctca tccacccctt gggagacttt
     6181 cctatgttca taaaagacaa ggtgaatcaa cttgaaggga cttttggtcc cagtgcaaag
     6241 aggtttttta gggttttaga tcaattcgac aacatacatg acttagtatt tgtgtatggc
     6301 tgttacagac attgggggca cccctatata gattatcgga agggtctgtc gaaactatat
     6361 gatcaagttc acattaagaa agtaatagat aagtcctacc aggagtgttt agcaagtgac
     6421 ttggccagaa ggatcctcag atggggattt gacaagtact ccaaatggta tctagattcg
     6481 agattccttg cccgagacca ccccttgact ccttatgtca agacccaaac atggccaccc
     6541 aaacatatag tagacttggt gggggacaca tggcataagc tcccgatcac gcagatcttt
     6601 gaaattcctg aatcaatgga cccgtcagag atactggatg acaaatcaca ttctttcacc
     6661 agaacaaggt tagcttcttg gctgtcagag aaccgagggg ggcctgttcc tagcgagaag
     6721 gtcattatca cggccctgtc taagccacct gtcaatcccc gagagttttt gaaatctata
     6781 gacctcggag gattgccaga tgaggatttg ataattggcc tcaaaccaaa ggaacgggag
     6841 ttgaagatcg agggccgatt ctttgctcta atgtcatgga atctaagatt atattttgtc
     6901 atcaccgaaa agctcttggc caactacatt ttgccacttt ttgacgcact gactatgaca
     6961 gacaacctga acaaggtgtt caaaaagttg atcgacaggg tcaccgggca agggcttttg
     7021 gactattcta gggtcacata cgcatttcac ctggactatg agaaatggaa caatcatcaa
     7081 agattggagt caacagagga tgtattctct gtcctagatc aggtgtttgg attgaagagg
     7141 gtgttttcta gaacacacga gttttttcag aagtcctgga tctattattc agacagatca
     7201 gacctcattg ggttatggga ggaccaaata tattgcttgg atatgtctaa cggcccaacc
     7261 tgctggaatg gccaagatgg cgggctagag ggcttgcggc agaagggctg gagtctagtc
     7321 agtttattaa tgatagatag agaatctcaa accaggaaca caagaaccaa gatactagct
     7381 caaggagaca accaggttct gtgtcctaca tacatgttgt caccgggatt gtctcaagag
     7441 gggcttctct atgagttaga gagcatatca aggaatgcac tctcaatata ccgagctatc
     7501 gaggaaggag catctaagct ggggctgatc atcaagaagg aagagaccat gtgtagttat
     7561 gactttctca tatatgggaa gaccccctta tttcgaggca acatattggt gcctgaatcc
     7621 aaaagatggg cccgagtctc ttgcatctct aacgaccaaa tagtcaacct cgccaatata
     7681 atgtcgacag tatccaccaa tgcgctgaca gtggcacaac actctcaatc tctgatcaaa
     7741 cctatgaggg attttctgct catgtcagta caggcagttt tccactacct gttgtttagc
     7801 ccaatcctaa aaggcagagt ttataagatt ctgagtgctg aaggggagag ctttctccta
     7861 gccatgtcgc ggataatcta cctagatcct tctttgggag gggtgtctgg aatgtctctc
     7921 gggaggttcc atatacgtca gttctcagac cctgtctctg aagggttgtc attctggaga
     7981 gagatctggt taggctctca tgagtcctgg attcacgcgt tgtgtcagga ggccgggaac
     8041 cccgatcttg gagagagaac actcgagagc ttcactcgcc ttttagaaga tcctactacc
     8101 ttaaatatca aaggaggggc cagccctacc attctactca aggatgctat cagaaaggct
     8161 ctgtacgacg aggtggacaa ggtggaaaat tcagagtttc gagaggcaat cctgctgtcc
     8221 aagacccata gagataactt tatactcttt ttaaaatctg ttgagcctct gttccctcga
     8281 tttctcagtg agctcttcag ttcgtctttc ttgggaatac cggagtcaat cattggactg
     8341 atacaaaact cccggacaat aagaaggcag tttagaaaga gtctctcaag aactttagaa
     8401 gagtccttct acaactcaga gatccacggg attaatcgga taacccagac acctcaaagg
     8461 gtcggaaggg tgtggccttg ctcttcagag agggcagatc tacttaggga gatctcttgg
     8521 gggaggaaag tggtaggcac gacagttcct cacccttccg agatgttgga gttgtttccc
     8581 aaatcctcca tttcctgcac ttgtggagca acagggggag gcaatcctag agtctctgta
     8641 tcagtactcc cgtccttcga tcagtcattt ttctcacggg gccccctaaa gggatacttg
     8701 ggctcgtcca cctccatgtc aacccagcta ttccatgcat gggaaaaagt cactaatgtt
     8761 catgtggtga aaagggctct atcgttaaaa gaatctataa actggttcat caataggaat
     8821 tccaatttgg ctcaaactct aattggaaac atcatgtctc tgacaggccc tgatttccct
     8881 ctagaagagg cccctgtttt caaacggaca gggtcagcct tgcataggtt caagtctgcc
     8941 agatacagcg aaggagggta ttcttctgtt tgccctaacc ttctctccca tatctctgtt
     9001 agtacagaca ctatgtctga tttgacccaa aacgggaaga actatgattt catgtttcag
     9061 ccattgatgc tttatgcgca aacatggaca tcggaactgg tacagaggga tacaagactt
     9121 agagactcca cgtttcactg gcaccttcgg tgcaacagat gtgtgaggcc cattgatgat
     9181 ataacactgg aaacttctca gatcttcgag ttcccggatg tgtcaaaaag gatatccagg
     9241 atggtttctg gagctgtccc tcaatttcag aagcttcctg atatccgtct aagaccaggt
     9301 gattttgaat ccctaagtgg tagagaaaag tctcgccata tagggtcagc tcaggggctc
     9361 ttatactcaa tcttagtagc aattcacgac tcaggataca atgatgggac catcttccct
     9421 gtcaacatat acggcaaagt ttcccccaga gactatttga gagggcttgc aagagggatc
     9481 ttgatagggt cctcgatttg cttcttgaca cgaatgacaa atattaacat taacagacct
     9541 cttgaattga tctcaggggt aatttcctat attctcctga ggctggataa tcatccctct
     9601 ctgtatataa tgcttagaga accgtctctt agaggagaaa tattctctat ccctcagaaa
     9661 atccccgccg cttacccaac cactatgaga gaaggcaaca gatcgatttt gtgttacctc
     9721 caacacgtgc tacgctatga gcgagaggca atcacggcgt ccccggagaa tgactggctg
     9781 tggatcttct cagacttcag aagtgtgaaa atgacgtact tgaccctcat tacctaccag
     9841 tctcacctcc tactccagag ggttgagaga aacttgtcta agagtatgag agctactctg
     9901 cgacaaatga gttccttaat gaggcaagtg ctgggtgggc acggagaaga taccttggag
     9961 tcagacgatg acattcaacg attactaaaa gactctttgc gaaagacaag gtgggtggat
    10021 caagaggtgc gccatgcagc tagaaccatg aatggagatt acagccccga caagaaagta
    10081 tcccacaagg caggatgttc agaatgggtc tgctctgctc aacagattgc cgtctccacc
    10141 tcagccaacc cggcccctgt ctcagagctt gacattaggg ccctctctaa gaggtttcaa
    10201 aaccccttga tctcgggcct gagagtggtt cagtgggcaa ccggtgccca ttataagctt
    10261 aagcctattc tagatgatct aaatgttttc ccatctctct gtcttgtaat tggagacggg
    10321 tcagggggaa tatcaagggc agttctcaac atgtttccag attctaagct tgtgttcaac
    10381 agcctattgg aggtgaatga tctgatggct tccggaacac atccactgcc tccttcagca
    10441 atcatgagtg gaggagatga catcatctcc agagtgatag actttgactc aatatgggag
    10501 aaaccgtccg acctgaggaa ctcggccacc tggagatact tccagtcggt tcaaaaacag
    10561 gtcaacatgt cgtatgacct cattatttgt gatgcagaag ttactgatat tgcatctatc
    10621 aaccggataa ctctgttgat gtctgatttc gcattgtcta tagatggacc actttatctg
    10681 gtcttcaaaa cttacgggac tatgctagta aacccggact ataaagctat tcaacatctg
    10741 tcaagagcgt tcccttcggt cacagggttt gtaacccaag taacttcatc cttttcttct
    10801 gagctatacc tccggttctc taaacgagga aagtttttca gggacgccga gtacttgacc
    10861 tcttccaccc ttcgagagat gagccttgtg ttgttcaatt gcagcagccc caaaagtgag
    10921 atgcagagag ctcgttcctt aaactatcaa gacctggtaa ggggatttcc tgaagagatc
    10981 atatcaaatc cttacaacga aatgatcata actctgattg acaatgatgt agagtccttc
    11041 ctagtccaca agatggtgga tgatcttgag ctacagaggg gaactctgtc taaagtggct
    11101 atcattatat ccatcatgat cgttttttcc aatagagtct tcaacatttc caaacctttg
    11161 actgacccct tgttctaccc cccatctgat cctaaaatcc tgaggcactt caacatatgt
    11221 tgcagtacta tgatgtatct atctaccgct ttaggcgacg tccctaactt cgcaagactt
    11281 catgacctgt ataacagacc tataacttgt tacttcagaa aacaagttat tcgagggaat
    11341 atttatctat cttggagttg gtccgatgat accccagtgt tcaagagagt agcctgtaat
    11401 tctagcttga gtctgtcatc tcactggatc aggttgatct acaagatagt gaagactacc
    11461 agactcattg gcagcataaa agacctatca ggagaggtag aacgacatct tcatgggtat
    11521 aacagatgga tcaccctcga ggatatccga tctagatcat ccctactaga ttacagttgt
    11581 ttgtaagccg gatattaccg aaagcctgtg catgctaaaa ttcttgtatg atgcatcttg
    11641 aaaaaaacaa gatcttgaat ccggacctct ggttgtttga ttgttttttc catctttatt
    11701 gtttttttgt taagcgtggg tcggcatggc atctccacct cctcgcggtc cgacctgggc
    11761 atccgaagga ggacgcacgt ccactcggat ggctaaggga gggcggggat ccggctgcta
    11821 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac
    11881 cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaagtcgcg cttggcgtaa
    11941 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
    12001 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
    12061 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
    12121 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
    12181 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
    12241 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
    12301 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
    12361 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
    12421 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
    12481 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
    12541 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
    12601 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
    12661 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
    12721 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
    12781 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
    12841 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
    12901 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
    12961 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
    13021 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
    13081 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
    13141 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
    13201 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacccttg
    13261 gaggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
    13321 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
    13381 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
    13441 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
    13501 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
    13561 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    13621 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    13681 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
    13741 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    13801 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    13861 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    13921 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    13981 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
    14041 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
    14101 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
    14161 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
    14221 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
    14281 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
    14341 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
    14401 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
    14461 gggattttgc cgatttcggc ctattggtta aaaaaatgag ctgatttaac aaaaatttaa
    14521 cgcgaatttt aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac
    14581 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
    14641 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
    14701 acgacggcca gtgagcgcgc ctagttatta atagtaatca attacggggt cattagttca
    14761 tagcccatat atggagttcc g
//
