LOCUS       sequence-483818-       12696 bp    DNA     circular SYN 19-AUG-2025
DEFINITION  Express H2B-EGFP in filamentous fungi.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12696)
  TITLE     A Toolbox of TEF1-Intron-Based Strong Promoter Reporter Systems for 
            Filamentous Fungi
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 19, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pGY219
FEATURES             Location/Qualifiers
     source          1..12696
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1723..1725,1726..2439)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(1723..1725,1726..2436)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1765..1786)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2026..2045)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2373..2394
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3262..4287
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANILFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(4359..4392)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(4862..4878)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4977..4994)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5148..5736)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5228..5247)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5858..6649,6650..6718))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6481..6500
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6719..6810)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6852..6871
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6877..6896
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    7103..7136
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     rep_origin      7449..12696
                     /label=AMA1
                     /note="replication origin from Aspergillus nidulans 
                     (Aleksenko and Clutterbuck, 1996)"
ORIGIN
        1 taacaatcta ttctccgctt ggtgatgcta aagggctttc aatagacctt gtaagtgaag
       61 gagatggagc cgtcaatccg ctaccctgcc tctggtcagt tggtctcagc aatgtaccct
      121 gtgagtcttg atagactagt tggtgactag tctctgtaga tggaacaaat ggtgcttgag
      181 agggcacgta tggcagagct ccagtcatct gcggaacata tactgggccc gggaagatct
      241 catggtcata gctgtttccg ctgagggttt aatcgttgac ctcacaggga tttccaccaa
      301 aaagtggacc gggcgggcgc cactcggccg tgccacagca gcctgagagc ggacaaataa
      361 caacagccgc ctgccgcggg gttcggttgc aaacatgacc aacaggccag gccatcatca
      421 acccaccgct gcgttgatgc ccaggatttc agtccaataa tccacaattt accaacggat
      481 agagctaggt gaactagata gacaggaggg ccagagggag gggaccgaga tgaaaaattt
      541 tcgatgaaag agtggtcaag gtggggtcgt agttcggcgc tccgagggcg aggaaccaag
      601 gaaaggcgag aaaaggacag gctgatcgcg ctgcgttgct gggctgcaag cgtgtccagt
      661 tgagtctgga aaaggctccg ccgtgaagat tctgcgttgg tcccgcacct gcgcggtggg
      721 ggcattaccc ctccatgtcc aatgatttca agtcaaagcc aagggttgaa gcccgcccgc
      781 ttagtcgcct tctcgcttga cccctccata taagtatttc ccctcctccc cctcccacaa
      841 atttttcctt tccctttcct ccctcgtccg cttcagtacg tatatcttcc ccccctctct
      901 cttccttctc actcttctct ccttctttct tgattcatcc tctctctaac tgacttcttt
      961 gctcagcacc tctacgcgtt ctggccgtag tatctgagca atttttctac agactttttc
     1021 tatctaattc caaaaaagaa cttcgagttc attcacaacc gtcatcatgg ggtgagtcaa
     1081 gacaacgatc atcatctgac aaaggaagga catgggctaa cggatatctt ctacaacagt
     1141 aagcctccca aagccgctga gaagaagccc agcaccggtg gcaaggcccc ggcaggcggc
     1201 aaggctcccg ctgagaagaa ggaagccggc aagaagactg ctgccgctgc ctccggcgac
     1261 aagaagaagc gtggaaagac caggaaggag acctactcgt cttacatcta caagggtgag
     1321 taatcttaag acttgtattc ctagtcaata gttgttgact gtgttatagt cttgaagcag
     1381 gtccaccctg ataccggtat ctcgactcgt gctatgtcca tcctgaactc ctttgtcaat
     1441 ggtatgtcgt gaatgaagca gggttatacc ttcagcaagc acctaacgcg gtctagatat
     1501 cttcgagcgt gtcgcgaccg aggcctcgaa acttgccgct tacaacaaga agtccaccat
     1561 ctcttcacgg gagatccaga cctcgtaagt cctctgtcga tcaagatagt agcaattcgc
     1621 taacacctct ctagtgtgag gctcatcctt cccggtgaat tggcgaagca cgcggtgtcg
     1681 gaaggcacca aggctgtcac gaagtactcg tcgtctgcca aagtgagcaa gggcgaggag
     1741 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     1801 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     1861 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     1921 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     1981 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     2041 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     2101 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     2161 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     2221 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     2281 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     2341 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     2401 gccgggatca ctctcggcat ggacgagctg tacaagtaag cggacattcg atttatgccg
     2461 ttatgacttc cttaaaaaag cctttacgaa tgaaagaaat ggaattagac ttgttatgta
     2521 gttgattcta caatggatta tgattcctga acttcaaatc cgctgttcat tattaatctc
     2581 agctcttccc gtaaagccaa tgttgaaact attcgtaaat gtacctcgtt ttgcgtgtac
     2641 cttgcttatc acgtgatatt acatgacctg gacagagttc tgcgcgaaag tcataacgta
     2701 aatcccgggc ggtaggtgcg tcccgggcgg aaggtagttt tctcgtccac cccaacgcgt
     2761 ttatcaacct caactttcaa caaccatcat gccaccaaaa gcgcgtaaaa caaagcgaga
     2821 tttgattgag caagagggca ggatccaatg cgcgattcaa gacattaaaa atggaaaatt
     2881 tcaaaaaatt gcgcccgcag cgcgtgcata caaaattcat cccaatacaa gtgtaatgct
     2941 agtggaggtc aacacatcaa tgcctatttt ggtttagtcg tccaggcggt gagcacaaaa
     3001 tttgtgtcgt ttgacaagat ggttcattta ggcaactggt cagatcagcc ccacttgtag
     3061 cagtagcggc ggcgctcgaa gtgtgactct tattagcaga caggaacgag gacattatta
     3121 tcatctgctg cttggtgcac gataacttgg tgcgtttgtc aagcaaggta agtggacgac
     3181 ccggtcatac cttcttaagt tcgcccttcc tccctttatt tcagattcaa tctgacttac
     3241 ctattctacc caagcatcca aatgaaaaag cctgaactca ccgcgacgtc tgtcgagaag
     3301 tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa
     3361 tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc
     3421 gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg
     3481 attccggaag tgcttgacat tggggagttc agcgagagcc tgacctattg catctcccgc
     3541 cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctccag
     3601 ccggtcgcgg aggccatgga tgcgattgct gcggccgatc ttagccagac gagcgggttc
     3661 ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg
     3721 attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc
     3781 gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac
     3841 ctcgtgcatg cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg
     3901 gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcctc
     3961 ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat
     4021 ccggagcttg caggatcgcc gcgcctccgg gcgtatatgc tccgcattgg tcttgaccaa
     4081 ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc
     4141 gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc
     4201 gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc
     4261 agcactcgtc cgagggcaaa ggaatagagt agatgccgac cgatccccac taccgcatta
     4321 agacctcagc gcggccgcaa atttaaataa aatgaagtga agttcctata ctttctagag
     4381 aataggaact tctatagtga gtcgaataag ggcgacacaa aatttattct aaatgcataa
     4441 taaatactga taacatctta tagtttgtat tatattttgt attatcgttg acatgtataa
     4501 ttttgatatc aaaaactgat tttcccttta ttattttcga gatttatttt cttaattctc
     4561 tttaacaaac tagaaatatt gtatatacaa aaaatcataa ataatagatg aatagtttaa
     4621 ttataggtgt tcatcaatcg aaaaagcaac gtatcttatt taaagtgcgt tgcttttttc
     4681 tcatttataa ggttaaataa ttctcatata tcaagcaaag tgacaggcgc ccttaaatat
     4741 tctgacaaat gctctttccc taaactcccc ccataaaaaa acccgccgaa gcgggttttt
     4801 acgttatttg cggattaacg attactcgtt atcagaaccg cccagggggc ccgagcttaa
     4861 gactggccgt cgttttacaa cacagaaaga gtttgtagaa acgcaaaaag gccatccgtc
     4921 aggggccttc tgcttagttt gatgcctggc agttccctac tctcgccttc cgcttcctcg
     4981 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     5041 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     5101 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     5161 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     5221 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     5281 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     5341 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     5401 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     5461 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     5521 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtgggctaa ctacggctac
     5581 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     5641 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     5701 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     5761 gggtctgacg ctcagtggaa cgacgcgcgc gtaactcacg ttaagggatt ttggtcatga
     5821 gcttgcgccg tcccgtcaag tcagcgtaat gctctgctta ccaatgctta atcagtgagg
     5881 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     5941 agataactac gatacgggag ggcttaccat ctggccccag cgctgcgatg ataccgcgag
     6001 aaccacgctc accggctccg gatttatcag caataaacca gccagccgga agggccgagc
     6061 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     6121 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatc gctacaggca
     6181 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     6241 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     6301 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcgctacata
     6361 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     6421 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     6481 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     6541 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     6601 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     6661 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatat
     6721 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     6781 tatttgaatg tatttagaaa aataaacaaa taggggtcag tgttacaacc aattaaccaa
     6841 ttctgaacat tatcgcgagc ccatttatac ctgaatatgg ctcataacac cccttgtttg
     6901 cctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
     6961 cgtagcgccg atggtagtgt ggggactccc catgcgagag tagggaactg ccaggcatca
     7021 aataaaacga aaggctcagt cgaaagactg ggcctttcgc ccgggctaat tatggggtgt
     7081 cgcccttatt cgactctata gtgaagttcc tattctctag aaagtatagg aacttctgaa
     7141 gtggggattt aaatgcggcc gcgctgaggg tttaatcgac gaagcagctg acggccagtg
     7201 ccaagcttaa cgcgtaccgg gcccagtata tgttccgcag atgactggag ctctgccata
     7261 cgtgccctct caagcaccat ttgttccatc tacagagact agtcaccaac tagtctatca
     7321 agactcacag ggtacattgc tgagaccaac tgaccagagg cagggtagcg gattgacggc
     7381 tccatctcct tcacttacaa ggtctattga aagcccttta gcatcaccaa gcggagaata
     7441 gattgttaag cttatttttt gtatactgtt ttgtgatagc acgaagtttt tccacggtat
     7501 cttgttaaaa atatatattt gtggcgggct tacctacatc aaattaataa gagactaatt
     7561 ataaactaaa cacacaagca agctacttta gggtaaaagt ttataaatgc ttttgacgta
     7621 taaacgttgc ttgtatttat tattacaatt aaaggtggat agaaaaccta gagactagtt
     7681 agaaactaat ctcaggtttg cgttaaacta aatcagagcc cgagaggtta acagaaccta
     7741 gaaggggact agatatccgg gtagggaaac aaaaaaaaaa acaagacagc cacatattag
     7801 ggagactagt tagaagctag ttccaggact aggaaaataa aagacaatga taccacagtc
     7861 tagttgacaa ctagatagat tctagattga ggccaaagtc tctgagatcc aggttagttg
     7921 caactaatac tagttagtat ctagtctcct ataactctga agctagaata acttactact
     7981 attatcctca ccactgttca gctgcgcaaa cggagtgatt gcaaggtgtt cagagactag
     8041 ttattgacta gtcagtgact agcaataact aacaaggtat taacctacca tgtctgccat
     8101 caccctgcac ttcctcgggc tcagcagcct tttcctcctc attttcatgc tcattttcct
     8161 tgtttaagac tgtgactagt caaagactag tccagaacca caaaggagaa atgtcttacc
     8221 actttcttca ttgcttgtct cttttgcatt atccatgtct gcaactagtt agagtctagt
     8281 tagtgactag tccgacgagg acttgcttgt ctccggattg ttggaggaac tctccagggc
     8341 ctcaagatcc acaacagagc cttctagaag actggtcaat aactagttgg tctttgtctg
     8401 agtctgactt acgaggttgc atactcgctc cctttgcctc gtcaatcgat gagaaaaagc
     8461 gccaaaactc gcaatatggc tttgaaccac acggtgctga gactagttag aatctagtcc
     8521 caaactagct tggatagctt acctttgccc tttgcgttgc gacaggtctt gcagggtatg
     8581 gttcctttct caccagctga tttagctgcc ttgctaccct cacggcggat ctgcataaag
     8641 agtggctaga ggttataaat tagcactgat cctaggtacg gggctgaatg taacttgccy
     8701 ttcctttctc atcgcgcggc aagacaggct tgctcaaatt cctaccagtc acaggggtat
     8761 gcacggcgta cggaccactt gaactagtca cagattagtt agcaactagt ctgcattgaa
     8821 tggctgtact tacgggccct cgccattgtc ctgatcattt ccagcttcac cctcgttgct
     8881 gcaaagtagt tagtgactag tcaaggacta gttgaaatgg gagaagaaac tcacgaattc
     8941 tcgacaccct tagtattgtg gtccttggac ttggtgctgc tatatattag ctaatacact
     9001 agttagactc acagaaactt acgcagctcg cttgcgcttc ttggtaggag tcggggttgg
     9061 gagaacagtg ccttcaaaca agccttcata ccatgctact tgactagtca gggactagtc
     9121 accaagtaat ctagatagga cttgcctttg gcctccatca gttccttcat agtgggaggt
     9181 ccattgtgca atgtaaactc catgccgtgg gagttcttat ccttcaagtg cttgaccaat
     9241 atgtttctgt tggcagaggg aacctgtcaa ctagttaata actagtcaga aactagtata
     9301 gcagtagact cactgtacgc ttgaggcatc ccttcactcg gcagtagact tcatatggat
     9361 ggatatcagg cacgccattg tcgtcctgtg gactagtcag taactaggct taaagctagt
     9421 cgggtcggct tactatcttg aaatccggca gcgtaagctc cccgtcctta actgcctcga
     9481 gatagtgaca gtactctggg gactttcgga gatcgttatc gcgaatgctc ggcatactaa
     9541 tcgttgacta gtcttggact agtcccgagc aaaaaggatt ggaggaggag gaggaaggtg
     9601 agagtgagac aaagagcgaa ataagagctt caaaggctat ctctaagcag tatgaaggtt
     9661 aagtatctag ttcttgacta gatttaaaag agatttcgac tagttatgta cctggagttt
     9721 ggatatagga atgtgttgtg gtaacgaaat gtaaggggga ggaaagaaaa agtcggtcaa
     9781 gaggtaactc taagtcggcc attccttttt gggaggcgct aaccataaac ggcatggtcg
     9841 acttagagtt agctcaggga atttagggag ttatctgcga ccaccgagga acggcggaat
     9901 gccaaagaat cccgatggag ctctagctgg cggttgacaa ccccaccttt tggcgtttct
     9961 gcggcgttgc aggcgggact ggatacttcg tagaaccaga aaggcaaggc agaacgcgct
    10021 cagcaagagt gttggaagtg atagcatgat gtgccttgtt aactaggtca aaatctgcag
    10081 tatgcttgat gttatccaaa gtgtgagaga ggaaggtcca aacatacacg attgggagag
    10141 ggcctaggta taagagtttt tgagtagaac gcatgtgagc ccagccatct cgaggagatt
    10201 aaacacgggc cggcatttga tggctatgtt agtaccccaa tggaaacggt gagagtccag
    10261 tggtcgcaga taactcccta aattccctga gctaactcta agtcgaccat gccgtttatg
    10321 gttagcgcct cccaaaaagg aatggccgac ttagagttac ctcttgaccg actttttctt
    10381 tcctccccct tacatttcgt taccacaaca cattcctata tccaaactcc aggtacataa
    10441 ctagtcgaaa tctcttttaa atctagtcaa gaactagata cttaaccttc atactgctta
    10501 gagatagcct ttgaagctct tatttcgctc tttgtctcac tctcaccttc ctcctcctcc
    10561 tccaatcctt tttgctcggg actagtccaa gactagtcaa cgattagtat gccgagcatt
    10621 cgcgataacg atctccgaaa gtccccagag tactgtcact atctcgaggc agttaaggac
    10681 ggggagctta cgctgccgga tttcaagata gtaagccgac ccgactagct ttaagcctag
    10741 ttactgacta gtccacagga cgacaatggc gtgcctgata tccatccata tgaagtctac
    10801 tgccgagtga agggatgcct caagcgtaca gtgagtctac tgctatacta gtttctgact
    10861 agttattaac tagttgacag gttccctctg ccaacagaaa catattggtc aagcacttga
    10921 aggacaagaa ctcccacggc atggagttta cattgcacaa tggacctccc actatgaagg
    10981 aactgatgga ggccaaaggc aagtcctatc tagattactt ggtgactagt ccctgactag
    11041 tcaagtagca tggtatgaag gcttgtttga aggcactgtt ctcccaaccc cgactcctac
    11101 caagaagcgc aagcgagctg cgtaagtttc tgtgagtcta actagtgtat tagctaatat
    11161 atagcagcac caagtccaag gaccacaata ctaagggtgt cgagaattcg tgagtttctt
    11221 ctcccatttc aactagtcct tgactagtca ctaactactt tgcagcaacg agggtgaagc
    11281 tggaaatgat caggacaatg gcgagggccc gtaagtacag ccattcaatg cagactagtt
    11341 gctaactaat ctgtgactag ttcaagtggt ccgtacgccg tgcatacccc tgtgactggt
    11401 aggaatttga gcaagcctgt cttgccgcgc gatgagaaag gaarggcaag ttacattcag
    11461 ccccgtacct aggatcagtg ctaatttata acctctagcc actctttatg cagatccgcc
    11521 gtgagggtag caaggcagct aaatcagctg gtgagaaagg aaccataccc tgcaagacct
    11581 gtcgcaacgc aaagggcaaa ggtaagctat ccaagctagt ttgggactag attctaacta
    11641 gtctcagcac cgtgtggttc aaagccatat tgcgagtttt ggcgcttttt ctcatcgatt
    11701 gacgaggcaa agggagcgag tatgcaacct cgtaagtcag actcagacaa agaccaacta
    11761 gttattgacc agtcttctag aaggctctgt tgtggatctt gaggccctgg agagttcctc
    11821 caacaatccg gagacaagca agtcctcgtc ggactagtca ctaactagac tctaactagt
    11881 tgcagacatg gataatgcaa aagagacaag caatgaagaa agtggtaaga catttctcct
    11941 ttgtggttct ggactagtct ttgactagtc acagtcttaa acaaggaaaa tgagcatgaa
    12001 aatgaggagg aaaaggctgc tgagcccgag gaagtgcagg gtgatggcag acatggtagg
    12061 ttaatacctt gttagttatt gctagtcact gactagtcaa taactagtct ctgaacacct
    12121 tgcaatcact ccgtttgcgc agctgaacag tggtgaggat aatagtagta agttattcta
    12181 gcttcagagt tataggagac tagatactaa ctagtattag ttgcaactaa cctggatctc
    12241 agagactttg gcctcaatct agaatctatc tagttgtcaa ctagactgtg gtatcattgt
    12301 cttttatttt cctagtcctg gaactagctt ctaactagtc tccctaatat gtggctgtct
    12361 tgtttttttt ttttgtttcc ctacccggat atctagtccc cttctaggtt ctgttaacct
    12421 ctcgggctct gatttagttt aacgcaaacc tgagattagt ttctaactag tctctaggtt
    12481 ttctatccac ctttaattgt aataataaat acaagcaacg tttatacgtc aaaagcattt
    12541 ataaactttt accctaaagt agcttgcttg tgtgtttagt ttataattag tctcttatta
    12601 atttgatgta ggtaagcccg ccacaaatat atatttttaa caagataccg tggaaaaact
    12661 tcgtgctatc acaaaacagt atacaaaaaa taagct
//
