LOCUS       sequence-483820-       12375 bp    DNA     circular SYN 19-AUG-2025
DEFINITION  Express H2B-NLS-EGFP in filamentous fungi.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12375)
  TITLE     A Toolbox of TEF1-Intron-Based Strong Promoter Reporter Systems for 
            Filamentous Fungi
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12375)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 19, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pGY251
FEATURES             Location/Qualifiers
     source          1..12375
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1402..1404,1405..2118)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(1402..1404,1405..2115)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1444..1465)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1705..1724)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2052..2073
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2941..3966
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANILFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(4038..4071)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(4541..4557)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4656..4673)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4827..5415)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4907..4926)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5537..6328,6329..6397))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6160..6179
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6398..6489)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6531..6550
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6556..6575
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    6782..6815
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     rep_origin      7128..12375
                     /label=AMA1
                     /note="replication origin from Aspergillus nidulans 
                     (Aleksenko and Clutterbuck, 1996)"
ORIGIN
        1 taacaatcta ttctccgctt ggtgatgcta aagggctttc aatagacctt gtaagtgaag
       61 gagatggagc cgtcaatccg ctaccctgcc tctggtcagt tggtctcagc aatgtaccct
      121 gtgagtcttg atagactagt tggtgactag tctctgtaga tggaacaaat ggtgcttgag
      181 agggcacgta tggcagagct ccagtcatct gcggaacata tactgggccc gggaagatct
      241 catggtcata gctgtttccg ctgagggttt aatcgttgac ctcacaggga tttccaccaa
      301 aaagtggacc gggcgggcgc cactcggccg tgccacagca gcctgagagc ggacaaataa
      361 caacagccgc ctgccgcggg gttcggttgc aaacatgacc aacaggccag gccatcatca
      421 acccaccgct gcgttgatgc ccaggatttc agtccaataa tccacaattt accaacggat
      481 agagctaggt gaactagata gacaggaggg ccagagggag gggaccgaga tgaaaaattt
      541 tcgatgaaag agtggtcaag gtggggtcgt agttcggcgc tccgagggcg aggaaccaag
      601 gaaaggcgag aaaaggacag gctgatcgcg ctgcgttgct gggctgcaag cgtgtccagt
      661 tgagtctgga aaaggctccg ccgtgaagat tctgcgttgg tcccgcacct gcgcggtggg
      721 ggcattaccc ctccatgtcc aatgatttca agtcaaagcc aagggttgaa gcccgcccgc
      781 ttagtcgcct tctcgcttga cccctccata taagtatttc ccctcctccc cctcccacaa
      841 atttttcctt tccctttcct ccctcgtccg cttcagtacg tatatcttcc ccccctctct
      901 cttccttctc actcttctct ccttctttct tgattcatcc tctctctaac tgacttcttt
      961 gctcagcacc tctacgcgtt ctggccgtag tatctgagca atttttctac agactttttc
     1021 tatctaattc caaaaaagaa cttcgagttc attcacaacc gtcatcatgg ggtgagtcaa
     1081 gacaacgatc atcatctgac aaaggaagga catgggctaa cggatatctt ctacaacagt
     1141 aagcctccca aagccgctga gaagaagccc agcaccggtg gcaaggcccc ggcaggcggc
     1201 aaggctcccg ctgagaagaa ggaagccggc aagaagactg ctgccgctgc ctccggcgac
     1261 aagaagaagc gtggaaagac caggaaggag acctactcgt cttacatcta caaggtcttg
     1321 aagcaggtcc accctgatac cggtatctcg actcgtgcta tgtccatcct gaactccttt
     1381 gtcaatgata tcttcgagcg tgtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     1441 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     1501 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     1561 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
     1621 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     1681 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     1741 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     1801 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     1861 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     1921 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     1981 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
     2041 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     2101 gacgagctgt acaagtaagc ggacattcga tttatgccgt tatgacttcc ttaaaaaagc
     2161 ctttacgaat gaaagaaatg gaattagact tgttatgtag ttgattctac aatggattat
     2221 gattcctgaa cttcaaatcc gctgttcatt attaatctca gctcttcccg taaagccaat
     2281 gttgaaacta ttcgtaaatg tacctcgttt tgcgtgtacc ttgcttatca cgtgatatta
     2341 catgacctgg acagagttct gcgcgaaagt cataacgtaa atcccgggcg gtaggtgcgt
     2401 cccgggcgga aggtagtttt ctcgtccacc ccaacgcgtt tatcaacctc aactttcaac
     2461 aaccatcatg ccaccaaaag cgcgtaaaac aaagcgagat ttgattgagc aagagggcag
     2521 gatccaatgc gcgattcaag acattaaaaa tggaaaattt caaaaaattg cgcccgcagc
     2581 gcgtgcatac aaaattcatc ccaatacaag tgtaatgcta gtggaggtca acacatcaat
     2641 gcctattttg gtttagtcgt ccaggcggtg agcacaaaat ttgtgtcgtt tgacaagatg
     2701 gttcatttag gcaactggtc agatcagccc cacttgtagc agtagcggcg gcgctcgaag
     2761 tgtgactctt attagcagac aggaacgagg acattattat catctgctgc ttggtgcacg
     2821 ataacttggt gcgtttgtca agcaaggtaa gtggacgacc cggtcatacc ttcttaagtt
     2881 cgcccttcct ccctttattt cagattcaat ctgacttacc tattctaccc aagcatccaa
     2941 atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac
     3001 agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat
     3061 gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat
     3121 cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt
     3181 ggggagttca gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg
     3241 caagacctgc ctgaaaccga actgcccgct gttctccagc cggtcgcgga ggccatggat
     3301 gcgattgctg cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga
     3361 atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat
     3421 cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag
     3481 ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcatgc ggatttcggc
     3541 tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg
     3601 atgttcgggg attcccaata cgaggtcgcc aacatcctct tctggaggcc gtggttggct
     3661 tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg
     3721 cgcctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac
     3781 ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga
     3841 gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc
     3901 tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag
     3961 gaatagagta gatgccgacc gatccccact accgcattaa gacctcagcg cggccgcaaa
     4021 tttaaataaa atgaagtgaa gttcctatac tttctagaga ataggaactt ctatagtgag
     4081 tcgaataagg gcgacacaaa atttattcta aatgcataat aaatactgat aacatcttat
     4141 agtttgtatt atattttgta ttatcgttga catgtataat tttgatatca aaaactgatt
     4201 ttccctttat tattttcgag atttattttc ttaattctct ttaacaaact agaaatattg
     4261 tatatacaaa aaatcataaa taatagatga atagtttaat tataggtgtt catcaatcga
     4321 aaaagcaacg tatcttattt aaagtgcgtt gcttttttct catttataag gttaaataat
     4381 tctcatatat caagcaaagt gacaggcgcc cttaaatatt ctgacaaatg ctctttccct
     4441 aaactccccc cataaaaaaa cccgccgaag cgggttttta cgttatttgc ggattaacga
     4501 ttactcgtta tcagaaccgc ccagggggcc cgagcttaag actggccgtc gttttacaac
     4561 acagaaagag tttgtagaaa cgcaaaaagg ccatccgtca ggggccttct gcttagtttg
     4621 atgcctggca gttccctact ctcgccttcc gcttcctcgc tcactgactc gctgcgctcg
     4681 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     4741 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     4801 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     4861 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     4921 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     4981 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     5041 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     5101 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     5161 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     5221 gctacagagt tcttgaagtg gtgggctaac tacggctaca ctagaagaac agtatttggt
     5281 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     5341 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     5401 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     5461 gacgcgcgcg taactcacgt taagggattt tggtcatgag cttgcgccgt cccgtcaagt
     5521 cagcgtaatg ctctgcttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     5581 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     5641 gcttaccatc tggccccagc gctgcgatga taccgcgaga accacgctca ccggctccgg
     5701 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     5761 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     5821 ttaatagttt gcgcaacgtt gttgccatcg ctacaggcat cgtggtgtca cgctcgtcgt
     5881 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     5941 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     6001 ccgcagtgtt atcactcatg gttatggcag cgctacataa ttctcttact gtcatgccat
     6061 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     6121 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     6181 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     6241 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     6301 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     6361 agggaataag ggcgacacgg aaatgttgaa tactcatatt cttccttttt caatattatt
     6421 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     6481 ataaacaaat aggggtcagt gttacaacca attaaccaat tctgaacatt atcgcgagcc
     6541 catttatacc tgaatatggc tcataacacc ccttgtttgc ctggcggcag tagcgcggtg
     6601 gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg
     6661 gggactcccc atgcgagagt agggaactgc caggcatcaa ataaaacgaa aggctcagtc
     6721 gaaagactgg gcctttcgcc cgggctaatt atggggtgtc gcccttattc gactctatag
     6781 tgaagttcct attctctaga aagtatagga acttctgaag tggggattta aatgcggccg
     6841 cgctgagggt ttaatcgacg aagcagctga cggccagtgc caagcttaac gcgtaccggg
     6901 cccagtatat gttccgcaga tgactggagc tctgccatac gtgccctctc aagcaccatt
     6961 tgttccatct acagagacta gtcaccaact agtctatcaa gactcacagg gtacattgct
     7021 gagaccaact gaccagaggc agggtagcgg attgacggct ccatctcctt cacttacaag
     7081 gtctattgaa agccctttag catcaccaag cggagaatag attgttaagc ttattttttg
     7141 tatactgttt tgtgatagca cgaagttttt ccacggtatc ttgttaaaaa tatatatttg
     7201 tggcgggctt acctacatca aattaataag agactaatta taaactaaac acacaagcaa
     7261 gctactttag ggtaaaagtt tataaatgct tttgacgtat aaacgttgct tgtatttatt
     7321 attacaatta aaggtggata gaaaacctag agactagtta gaaactaatc tcaggtttgc
     7381 gttaaactaa atcagagccc gagaggttaa cagaacctag aaggggacta gatatccggg
     7441 tagggaaaca aaaaaaaaaa caagacagcc acatattagg gagactagtt agaagctagt
     7501 tccaggacta ggaaaataaa agacaatgat accacagtct agttgacaac tagatagatt
     7561 ctagattgag gccaaagtct ctgagatcca ggttagttgc aactaatact agttagtatc
     7621 tagtctccta taactctgaa gctagaataa cttactacta ttatcctcac cactgttcag
     7681 ctgcgcaaac ggagtgattg caaggtgttc agagactagt tattgactag tcagtgacta
     7741 gcaataacta acaaggtatt aacctaccat gtctgccatc accctgcact tcctcgggct
     7801 cagcagcctt ttcctcctca ttttcatgct cattttcctt gtttaagact gtgactagtc
     7861 aaagactagt ccagaaccac aaaggagaaa tgtcttacca ctttcttcat tgcttgtctc
     7921 ttttgcatta tccatgtctg caactagtta gagtctagtt agtgactagt ccgacgagga
     7981 cttgcttgtc tccggattgt tggaggaact ctccagggcc tcaagatcca caacagagcc
     8041 ttctagaaga ctggtcaata actagttggt ctttgtctga gtctgactta cgaggttgca
     8101 tactcgctcc ctttgcctcg tcaatcgatg agaaaaagcg ccaaaactcg caatatggct
     8161 ttgaaccaca cggtgctgag actagttaga atctagtccc aaactagctt ggatagctta
     8221 cctttgccct ttgcgttgcg acaggtcttg cagggtatgg ttcctttctc accagctgat
     8281 ttagctgcct tgctaccctc acggcggatc tgcataaaga gtggctagag gttataaatt
     8341 agcactgatc ctaggtacgg ggctgaatgt aacttgcctt tcctttctca tcgcgcggca
     8401 agacaggctt gctcaaattc ctaccagtca caggggtatg cacggcgtac ggaccacttg
     8461 aactagtcac agattagtta gcaactagtc tgcattgaat ggctgtactt acgggccctc
     8521 gccattgtcc tgatcatttc cagcttcacc ctcgttgctg caaagtagtt agtgactagt
     8581 caaggactag ttgaaatggg agaagaaact cacgaattct cgacaccctt agtattgtgg
     8641 tccttggact tggtgctgct atatattagc taatacacta gttagactca cagaaactta
     8701 cgcagctcgc ttgcgcttct tggtaggagt cggggttggg agaacagtgc cttcaaacaa
     8761 gccttcatac catgctactt gactagtcag ggactagtca ccaagtaatc tagataggac
     8821 ttgcctttgg cctccatcag ttccttcata gtgggaggtc cattgtgcaa tgtaaactcc
     8881 atgccgtggg agttcttatc cttcaagtgc ttgaccaata tgtttctgtt ggcagaggga
     8941 acctgtcaac tagttaataa ctagtcagaa actagtatag cagtagactc actgtacgct
     9001 tgaggcatcc cttcactcgg cagtagactt catatggatg gatatcaggc acgccattgt
     9061 cgtcctgtgg actagtcagt aactaggctt aaagctagtc gggtcggctt actatcttga
     9121 aatccggcag cgtaagctcc ccgtccttaa ctgcctcgag atagtgacag tactctgggg
     9181 actttcggag atcgttatcg cgaatgctcg gcatactaat cgttgactag tcttggacta
     9241 gtcccgagca aaaaggattg gaggaggagg aggaaggtga gagtgagaca aagagcgaaa
     9301 taagagcttc aaaggctatc tctaagcagt atgaaggtta agtatctagt tcttgactag
     9361 atttaaaaga gatttcgact agttatgtac ctggagtttg gatataggaa tgtgttgtgg
     9421 taacgaaatg taagggggag gaaagaaaaa gtcggtcaag aggtaactct aagtcggcca
     9481 ttcctttttg ggaggcgcta accataaacg gcatggtcga cttagagtta gctcagggaa
     9541 tttagggagt tatctgcgac caccgaggaa cggcggaatg ccaaagaatc ccgatggagc
     9601 tctagctggc ggttgacaac cccacctttt ggcgtttctg cggcgttgca ggcgggactg
     9661 gatacttcgt agaaccagaa aggcaaggca gaacgcgctc agcaagagtg ttggaagtga
     9721 tagcatgatg tgccttgtta actaggtcaa aatctgcagt atgcttgatg ttatccaaag
     9781 tgtgagagag gaaggtccaa acatacacga ttgggagagg gcctaggtat aagagttttt
     9841 gagtagaacg catgtgagcc cagccatctc gaggagatta aacacgggcc ggcatttgat
     9901 ggctatgtta gtaccccaat ggaaacggtg agagtccagt ggtcgcagat aactccctaa
     9961 attccctgag ctaactctaa gtcgaccatg ccgtttatgg ttagcgcctc ccaaaaagga
    10021 atggccgact tagagttacc tcttgaccga ctttttcttt cctccccctt acatttcgtt
    10081 accacaacac attcctatat ccaaactcca ggtacataac tagtcgaaat ctcttttaaa
    10141 tctagtcaag aactagatac ttaaccttca tactgcttag agatagcctt tgaagctctt
    10201 atttcgctct ttgtctcact ctcaccttcc tcctcctcct ccaatccttt ttgctcggga
    10261 ctagtccaag actagtcaac gattagtatg ccgagcattc gcgataacga tctccgaaag
    10321 tccccagagt actgtcacta tctcgaggca gttaaggacg gggagcttac gctgccggat
    10381 ttcaagatag taagccgacc cgactagctt taagcctagt tactgactag tccacaggac
    10441 gacaatggcg tgcctgatat ccatccatat gaagtctact gccgagtgaa gggatgcctc
    10501 aagcgtacag tgagtctact gctatactag tttctgacta gttattaact agttgacagg
    10561 ttccctctgc caacagaaac atattggtca agcacttgaa ggacaagaac tcccacggca
    10621 tggagtttac attgcacaat ggacctccca ctatgaagga actgatggag gccaaaggca
    10681 agtcctatct agattacttg gtgactagtc cctgactagt caagtagcat ggtatgaagg
    10741 cttgtttgaa ggcactgttc tcccaacccc gactcctacc aagaagcgca agcgagctgc
    10801 gtaagtttct gtgagtctaa ctagtgtatt agctaatata tagcagcacc aagtccaagg
    10861 accacaatac taagggtgtc gagaattcgt gagtttcttc tcccatttca actagtcctt
    10921 gactagtcac taactacttt gcagcaacga gggtgaagct ggaaatgatc aggacaatgg
    10981 cgagggcccg taagtacagc cattcaatgc agactagttg ctaactaatc tgtgactagt
    11041 tcaagtggtc cgtacgccgt gcatacccct gtgactggta ggaatttgag caagcctgtc
    11101 ttgccgcgcg atgagaaagg aagggcaagt tacattcagc cccgtaccta ggatcagtgc
    11161 taatttataa cctctagcca ctctttatgc agatccgccg tgagggtagc aaggcagcta
    11221 aatcagctgg tgagaaagga accataccct gcaagacctg tcgcaacgca aagggcaaag
    11281 gtaagctatc caagctagtt tgggactaga ttctaactag tctcagcacc gtgtggttca
    11341 aagccatatt gcgagttttg gcgctttttc tcatcgattg acgaggcaaa gggagcgagt
    11401 atgcaacctc gtaagtcaga ctcagacaaa gaccaactag ttattgacca gtcttctaga
    11461 aggctctgtt gtggatcttg aggccctgga gagttcctcc aacaatccgg agacaagcaa
    11521 gtcctcgtcg gactagtcac taactagact ctaactagtt gcagacatgg ataatgcaaa
    11581 agagacaagc aatgaagaaa gtggtaagac atttctcctt tgtggttctg gactagtctt
    11641 tgactagtca cagtcttaaa caaggaaaat gagcatgaaa atgaggagga aaaggctgct
    11701 gagcccgagg aagtgcaggg tgatggcaga catggtaggt taataccttg ttagttattg
    11761 ctagtcactg actagtcaat aactagtctc tgaacacctt gcaatcactc cgtttgcgca
    11821 gctgaacagt ggtgaggata atagtagtaa gttattctag cttcagagtt ataggagact
    11881 agatactaac tagtattagt tgcaactaac ctggatctca gagactttgg cctcaatcta
    11941 gaatctatct agttgtcaac tagactgtgg tatcattgtc ttttattttc ctagtcctgg
    12001 aactagcttc taactagtct ccctaatatg tggctgtctt gttttttttt tttgtttccc
    12061 tacccggata tctagtcccc ttctaggttc tgttaacctc tcgggctctg atttagttta
    12121 acgcaaacct gagattagtt tctaactagt ctctaggttt tctatccacc tttaattgta
    12181 ataataaata caagcaacgt ttatacgtca aaagcattta taaactttta ccctaaagta
    12241 gcttgcttgt gtgtttagtt tataattagt ctcttattaa tttgatgtag gtaagcccgc
    12301 cacaaatata tatttttaac aagataccgt ggaaaaactt cgtgctatca caaaacagta
    12361 tacaaaaaat aagct
//
