LOCUS       sequence-483821-       10416 bp    DNA     circular SYN 19-AUG-2025
DEFINITION  Sleeping Beauty XPRESSO vector for CaViar expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10416)
  TITLE     XPRESSO: Rapid Genetic Engineering of Human Pluripotent Stem Cells 
            for Durable Overexpression Using a Modular Anti-silencing Vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10416)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 19, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pSB-XPRESSO-CaViar
FEATURES             Location/Qualifiers
     source          1..10416
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    324..548
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     537..554
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     misc_feature    587..1175
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             693..704
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1102..1122
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(1233..1673,1674..1679,1680..2114,2115..2132,
                     2133..2402,2403..2408,2409..2465,2466..2492,2493..2516,
                     2517..2519,2520..2552,2553..2555,2556..2573,2574..2582))
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6f
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEEFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFGVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     primer_bind     1789..1808
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2048..2069
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2175..2196)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      2477..2486
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(2676..3446)
                     /codon_start=1
                     /product="light-driven proton pump archaerhodopsin-3 from 
                     Halorubrum sodomense (Chow et al., 2010)"
                     /label=Arch
                     /translation="DPIALQAGYDLLGDGRPETLWLGIGTLLMLIGTFYFLVRGWGVTD
                     KDAREYYAVTILVSGIASAAYLSMFFGIGLTEVSVGGEMLDIYYARYAQWLFTTPLLLL
                     HLALLAKVDRVTIGTLVGVDALMIVTGLIGALSHTAIARYSWWLFSTICMIVVLYVLAT
                     SLRSAAKERGPEVASTFNTLTALVLVLWTAYPILWIIGTEGAGVVGLGIETLLFMVLDV
                     TAKVGFGFILLRSRAILGDTEAPEPSAGADVSAAD"
     CDS             complement(3447..3488)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     regulatory      3488..3497
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3562..3581)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          3589..4605
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(3643..3665)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(4606..4882)
                     /label=chicken beta-actin promoter
     enhancer        4884..5263
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             6434..7135
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MLSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(6567..6585)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6835..6854
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     7029..7052
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             7145..7201
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7202..7801
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7202..7221)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7698..7718
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(7901..7920)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7966..8021
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8020..8039)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8413..8430)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8584..9172)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8664..8683)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9343..10134,10135..10203))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9966..9985
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10204..10308)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggatccctat acagttgaag tcggaagttt acatacactt aagttggagt cattaaaact
       61 cgtttttcaa ctactccaca aatttcttgt taacaaacaa tagttttggc aagtcagtta
      121 ggacatctac tttgtgcatg acacaagtca tttttccaac aattgtttac agacagatta
      181 tttcacttat aattcactgt atcacaattc cagtgggtca gaagtttaca tacactaagt
      241 tcgactcctc tgcagaatgc ggcgatgttt cggtaagggg tccgctatct agtaggccca
      301 gctggttctt tccgcctcag aagccataga gcccaccgca tccccagcat gcctgctatt
      361 gtcttcccaa tcctccccct tgctgtcctg ccccacccca ccccccagaa tagaatgaca
      421 cctactcaga caatgcgatg caatttcctc attttattag gaaaggacag tgggagtggc
      481 accttccagg gtcaaggaag gcacggggga ggggcaaaca acagatggct ggcaactaga
      541 aggcacagtc gaggctgatc agcgagctca ggtcgacggt atcgatgcgg ggaggcggcc
      601 caaagggaga tccgactcgt ctgagggcga aggcgaagac gcggaagagg ccgcagagcc
      661 ggcagcaggc cgcgggaagg aaggtccgct ggattgaggg ccgaagggac gtagcagaag
      721 gacgtcccgc gcagaatcca ggtggcaaca caggcgagca gccaaggaaa ggacgatgat
      781 ttccccgaca acaccacgga attgtcagtg cccaacagcc gagcccctgt ccagcagcgg
      841 gcaaggcagg cggcgatgag ttccgccgtg gcaataggga gggggaaagc gaaagtcccg
      901 gaaaggagct gacaggtggt ggcaatgccc caaccagtgg gggttgcgtc agcaaacaca
      961 gtgcacacca cgccacgttg cctgacaacg ggccacaact cctcataaag agacagcaac
     1021 caggatttat acaaggagga gaaaatgaaa gccatacggg aagcaatagc atgatacaaa
     1081 ggcattaaag cagcgtatcc acatagcgta aaaggagcaa catagttaag aataccagtc
     1141 aatctttcac aaattttgta atccagaggt tgattatcga aagcttggcc tgacaggccg
     1201 tcttacacct cgttctcgta gcagaacttg tacttcgctg tcatcatttg tacaaactct
     1261 tcgtagttta cctgaccatc cccatcgatg tctgcttccc tgatcatttc atcaacctct
     1321 tcatctgtta acttctctcc aaggtttgtc atcacgtggc gaagctctgc tgcactgatg
     1381 tagccattgc catccttatc aaacacaccg aacgcttctc taatttcttc ttccgtgtcc
     1441 ctgtatttca tttttcttgc catcattgtc aggaactcag ggaagtcgat tgtgccgtca
     1501 ccgtcggcat ctacttcatt gatcatgtcc tgcagctctg cttctgtggg gttctgcccc
     1561 agagaccgca tcaccgtccc cagctccttg gttgttattg tcccatcccc gtccttgtca
     1621 aatagggaga attcctcttt aaattctgcg atctgctctt cagtcagttg gtccggcagg
     1681 ttgtactcca gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat gcccttcagc
     1741 tcgatgcggt tcaccagggt gtcgccctcg aacttcacct cggcgcgggt cttgtagttg
     1801 ccgtcgtcct tgaagaagat ggtgcgctcc tggatgtagc cttcgggcat ggcggacttg
     1861 aagaagtcgt gctgcttcat gtggtcgggg tagcggctga agcactgcac gccgtaggtc
     1921 agggtggtca cgagggtggg ccagggcacg ggcagcttgc cggtggtgca gatgaacttc
     1981 agggtcagct tgccgtaggt ggcatcgccc tcaccctcgc cggacacgct gaacttgtgg
     2041 ccgtttacgt cgccgtccag ctcgaccagg atgggcacca ccccggtgaa cagctcctcg
     2101 cccttgctca ccatgctccc tccggtaccg cccttgtaca gctcgtccat gccgagagtg
     2161 atcccggcgg cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggggtct
     2221 ttcgaaagtt tggactgcac gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg
     2281 ccgatggggg tgttctgctg gtagtggtag gcgagctgca cgccgccgtc ctcgatgttg
     2341 tggcggatct tgaagttcgc cttgatgccg ttcttctgct tgtcggcctt gatatagacg
     2401 ttctcgagtg agctcagccg acctatagct ctgactgcgt gacctgtctt attccactta
     2461 cgacgtgatg agtcgaccat ggtggcgaga tccttatcgt catcgtcgta cagatcccga
     2521 cccatttgct gtccaccagt catgctagcc ataccatgat gatgatgatg atgagaaccc
     2581 atcacgttga tgtcgatctg gtccaggggg atgtactcgc cctcgctggt gatcctgctc
     2641 ttggcggccg ccttgctcac tgctactacc ggtcggtcgg cggcactgac atcggcaccg
     2701 gcactgggtt ctggtgcctc ggtgtcgccc agaatagccc gggatctcaa caggataaag
     2761 ccaaagccga ccttggcagt cacgtccaac accataaaca gcagagtttc gatgcccagg
     2821 cccaccacgc cagcgccctc agtgcctatg atccacagga tagggtaagc ggtccacagc
     2881 accaagacca gagctgtcag ggtgttaaag gtagatgcca cctcggggcc ccgctccttt
     2941 gcagcagatc gcagggatgt agccagaaca tagagcacca ctatcatgca aattgtagag
     3001 aacaaccacc aactgtatct ggctatggcc gtgtggctca aggctccgat gaggccagtg
     3061 acgatcatca gggcgtccac acccaccagg gtgccgatgg tcacccgatc caccttagcg
     3121 agaagggcca ggtgcagcag cagaagtggg gtggtaaaca gccattgggc gtacctggca
     3181 taatagatat ccaacatttc gcccccgacg gacacctcag taagcccgat accaaagaac
     3241 atagacagat atgcggcgga tgcgattccg gacaccagga tagtcacagc gtaatattcc
     3301 cgggcatcct tatcggtgac tccccatccg cggaccagaa agtagaaggt tccaatcagc
     3361 atcagcagag tgccgatgcc cagccacagg gtttcaggtc tgccgtcacc cagtaggtcg
     3421 taaccagcct gcagagcgat ggggtcggtg ctgtccaggc ccagcagggg gttggggatg
     3481 ggcttgccca tggtggcggc ctcagaggcc aacagcggcc gcgatatcct cgaggaattc
     3541 tttgccaaaa tgatgagaca gcacaataac cagcacgttg cccaggagct gtaggaaaaa
     3601 gaagaaggca tgaacatggt tagcagaggc tctagagccg ccggtcacac gccagaagcc
     3661 gaaccccgcc ctgccccgtc ccccccgaag gcagccgtcc ccccgcggac agccccgagg
     3721 ctggagaggg agaaggggac ggcggcgcgg cgacgcacga aggccctccc cgcccatttc
     3781 cttcctgccg gcgccgcacc gcttcgcccc gcgcccgcta gagggggtgc ggcggcgcct
     3841 cccagatttc ggctccgcac agatttggga caaaggaagt ccctgcgccc tctcgcacga
     3901 ttaccataaa aggcaatggc tgcggctcgc cgcgcctcga cagccgccgg cgctccgggg
     3961 gccgccgcgc ccctcccccg agcccycccc ggcccgmggc ggccccgccc cgcccggcac
     4021 ccccacctgc cgccaccccc cgcccggcac ggcgagcccc gcgccacgcc ccgtacggag
     4081 ccccgcaccc gaagccgggc cgtgctcagc aactcgggga ggggggtgca gggggggttg
     4141 cagcccgacc gacgcgccca caccccctgc tcaccccccc acgcacacac cccgcacgca
     4201 gcctttgttc ccctcgcagc cccccccgca ccgcggggca ccgcccccgg ccgcgctccc
     4261 ctcgcgcaca ctgcggagcg cacaaagccc cgcgccgcgc ccgcagcgct cacagccgcc
     4321 gggcagcgcg gagccgcacg cggcgctccc cacgcacaca cacacgcacg caccccccga
     4381 gccgctcccc ccgcacaaag ggccctcccg gagcccctca aggctttcac gcagccacag
     4441 aaaagaaaca agccgtcatt aaaccaagcg ctaattacag cccggaggag aagggccgtc
     4501 ccgcccgctc acctgtggga gtaacgcggt cagtcagagc cggggcgggc ggcgcgaggc
     4561 ggcggcggag cggggcacgg ggcgaaggca gcgcgcagcg actcccgccc gccgcgcgct
     4621 tcgcttttta tagggccgcc gccgccgccg cctcgccata aaaggaaact ttcggagcgc
     4681 gccgctctga ttggctgccg ccgcacctct ccscctcscc ccsccccscc cctccccccc
     4741 cccccccccc cctgggcgcg cccccccccc ccccccgccc ccatcgctgc acaaaataat
     4801 taaaaaataa ataaatacaa aattgggggt ggggaggggg gggagatggg gagagtgaag
     4861 cagaacgtgg ggctcacctc gaccatggta atagcgatga ctaatacgta gatgtactgc
     4921 caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg
     4981 tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg
     5041 gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact
     5101 atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc
     5161 gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac
     5221 cccgtaattg attactatta ataactagtc aataatcaat gtcgactcgt attcccgggc
     5281 tgccaagcag aaaagccacc gctgaggaga ctccggtcac tgtcctcgcc ccgcctcccc
     5341 cttccctccc cttggggacc accgggcgcc acgccgcgaa cggtaagtgc cgcggtcgtc
     5401 ggcgcctccg ccctccccct agggccccaa ttcccagcgg gcgcggcgcc ggcccctccc
     5461 cccgccgcgc gcgcgcccgc tgccccgccc ttcgtggccg cccggcgtgg gcggtgccac
     5521 ccctcccccc ggcggccccg cgcgcagctc ccggctccct cccccttcgg atgtggcttg
     5581 agctgtaggc gcggagggcc ggagacgctg cagacccgcg acccggagca gctcggaggc
     5641 ggtgaagtcg gtggctttcc ttctctctag ctctcgctcg ctggtggtgc ttcagatgcc
     5701 acacgcgtag cttataatac cggttcaatt gccgacccct ccccccaact tctcggggac
     5761 tgtgggcgat gtgcgctctg cccactgacg ggcaccggag cctctaggcg agaccctgtc
     5821 tcacaaaata aagtaagccc ggactgagtg cggaaaggcg ggcctggcgg gtctggtctc
     5881 cccatgcggg ccaccagagg ccctgcagcc ttcagtcgct tgaaggggta atggcgcttc
     5941 cactcacaaa catggcggac agagcgtgtg aacgagatga acagcccctc aaaaatatgg
     6001 ccgccgaggc tggacggccg tgccccagca gcaccgcctc cgcgccccac gtgatctctc
     6061 gccgggcaca gcgctgaccg cggaggtcca accggaagaa tgtccggatt ggacattcgg
     6121 aagagggccc gccttccctg gggaatctct gcgcacgcgc agaacgcttc gaccaatgaa
     6181 aacacaggaa gccgtccgcg caaccgcgtt gcgtcacttc tgccgcccct gtttcaaggt
     6241 atatagccgt agacggaact tcgcctttct ctcggcctta gcgccatttt tttgggtgag
     6301 tgttttttgg ttcctgcgtt gggattccgt gtacaatcca tagacatctg acctcggcac
     6361 ttagcatcat cacagcaaac taactgtagc ctttctctct ttccctgtag aaacctctgc
     6421 acctgaggcc accatgttga gcaagggcga ggaggtcatc aaagagttca tgcgcttcaa
     6481 ggtgcgcatg gagggctcca tgaacggcca tgagttcgag atcgagggcg agggcgaggg
     6541 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggcg gccccctgcc
     6601 cttcgcctgg gacatcctgt ccccccagtt catgtacggc tccaaggcgt acgtgaagca
     6661 ccccgccgac atccccgatt acaagaagct gtccttcccc gagggcttca agtgggagcg
     6721 cgtgatgaac ttcgaggacg gcggtctggt gaccgtgacc caggactcct ccctgcagga
     6781 cggcacgctg atctacaagg tgaagatgcg cggcaccaac ttcccccccg acggccccgt
     6841 aatgcagaag aagacaatgg gctgggaggc ctccaccgag cgcctgtacc cccgcgacgg
     6901 cgtgctgaag ggcgagatcc accaggccct gaagctgaag gacggcggcc actacctggt
     6961 ggagttcaag accatctaca tggccaagaa gcccgtgcaa ctgcccggct actactacgt
     7021 ggacaccaag ctggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
     7081 gcgctccgag ggccgccacc acctgttcct gtacggcatg gacgagctgt acaagggcag
     7141 tggagctact aacttcagcc tgctgaagca ggctggtgac gtcgaggaga atcctggccc
     7201 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     7261 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     7321 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     7381 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     7441 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     7501 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     7561 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     7621 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     7681 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     7741 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     7801 attcgaaggc ctgtcgtgaa ttcactcctc aggtgcaggc tgcctatcag aaggtggtgg
     7861 ctggtgtggc caatgccctg gctcacaaat accactgaga tctttttccc tctgccaaaa
     7921 attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta
     7981 ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga
     8041 gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca acatatgcca
     8101 tatgctggct gccatgaact agctactcgg gaccccttac cgaaacatcg ccgcattctg
     8161 cagaggagtc gagtgtatgt aaacttctga cccactggga atgtgatgaa agaaataaaa
     8221 gctgaaatga atcattctct ctactattat tctgatattt cacattctta aaataaagtg
     8281 gtgatcctaa ctgacctaag acagggaatt tttactagga ttaaatgtca ggaattgtga
     8341 aaaagtgagt ttaaatgtat ttggctaagg tgtatgtaaa cttccgactt caactgtata
     8401 gggatccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     8461 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     8521 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     8581 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     8641 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     8701 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     8761 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     8821 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     8881 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     8941 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     9001 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     9061 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     9121 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     9181 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     9241 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     9301 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     9361 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     9421 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     9481 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     9541 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     9601 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     9661 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     9721 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     9781 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     9841 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     9901 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     9961 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    10021 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    10081 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    10141 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    10201 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    10261 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    10321 aaaagtgcca cctgatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
    10381 tcaggaaatt gtaagcgtta atattttgtt aaaatt
//
