LOCUS       sequence-483841-       12075 bp    DNA     circular SYN 19-AUG-2025
DEFINITION  Express mRFP1 in filamentous fungi.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12075)
  TITLE     A Toolbox of TEF1-Intron-Based Strong Promoter Reporter Systems for 
            Filamentous Fungi
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12075)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 19, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pGY209
FEATURES             Location/Qualifiers
     source          1..12075
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1144..1818
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="ASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKMR
                     LKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHST
                     GA"
     CDS             1144..1818
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="ASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKMR
                     LKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHST
                     GA"
     primer_bind     complement(1271..1289)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1539..1558
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1733..1756
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             2641..3666
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANILFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(3738..3771)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(4241..4257)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4356..4373)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4527..5115)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4607..4626)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5237..6028,6029..6097))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5860..5879
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6098..6189)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6231..6250
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6256..6275
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    6482..6515
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     rep_origin      6828..12075
                     /label=AMA1
                     /note="replication origin from Aspergillus nidulans 
                     (Aleksenko and Clutterbuck, 1996)"
ORIGIN
        1 taacaatcta ttctccgctt ggtgatgcta aagggctttc aatagacctt gtaagtgaag
       61 gagatggagc cgtcaatccg ctaccctgcc tctggtcagt tggtctcagc aatgtaccct
      121 gtgagtcttg atagactagt tggtgactag tctctgtaga tggaacaaat ggtgcttgag
      181 agggcacgta tggcagagct ccagtcatct gcggaacata tactgggccc gggaagatct
      241 catggtcata gctgtttccg ctgagggttt aatcgttgac ctcacaggga tttccaccaa
      301 aaagtggacc gggcgggcgc cactcggccg tgccacagca gcctgagagc ggacaaataa
      361 caacagccgc ctgccgcggg gttcggttgc aaacatgacc aacaggccag gccatcatca
      421 acccaccgct gcgttgatgc ccaggatttc agtccaataa tccacaattt accaacggat
      481 agagctaggt gaactagata gacaggaggg ccagagggag gggaccgaga tgaaaaattt
      541 tcgatgaaag agtggtcaag gtggggtcgt agttcggcgc tccgagggcg aggaaccaag
      601 gaaaggcgag aaaaggacag gctgatcgcg ctgcgttgct gggctgcaag cgtgtccagt
      661 tgagtctgga aaaggctccg ccgtgaagat tctgcgttgg tcccgcacct gcgcggtggg
      721 ggcattaccc ctccatgtcc aatgatttca agtcaaagcc aagggttgaa gcccgcccgc
      781 ttagtcgcct tctcgcttga cccctccata taagtatttc ccctcctccc cctcccacaa
      841 atttttcctt tccctttcct ccctcgtccg cttcagtacg tatatcttcc ccccctctct
      901 cttccttctc actcttctct ccttctttct tgattcatcc tctctctaac tgacttcttt
      961 gctcagcacc tctacgcgtt ctggccgtag tatctgagca atttttctac agactttttc
     1021 tatctaattc caaaaaagaa cttcgagttc attcacaacc gtcatcatgg ggtgagtcaa
     1081 gacaacgatc atcatctgac aaaggaagga catgggctaa cggatatctt ctacaacagt
     1141 aaggcctcct ccgaggacgt catcaaggag ttcatgcgct tcaaggtgcg catggagggc
     1201 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     1261 acccagaccg ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc
     1321 ctgtcccctc agttccagta cggctccaag gcctacgtga agcaccccgc cgacatcccc
     1381 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     1441 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
     1501 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
     1561 atgggctggg aggcctccac cgagcggatg taccccgagg acggcgccct gaagggcgag
     1621 atcaagatga ggctgaagct gaaggacggc ggccactacg acgccgaggt caagaccacc
     1681 tacatggcca agaagcccgt gcagctgccc ggcgcctaca agaccgacat caagctggac
     1741 atcacctccc acaacgagga ctacaccatc gtggaacagt acgagcgcgc cgagggccgc
     1801 cactccaccg gcgcctaagc ggacattcga tttatgccgt tatgacttcc ttaaaaaagc
     1861 ctttacgaat gaaagaaatg gaattagact tgttatgtag ttgattctac aatggattat
     1921 gattcctgaa cttcaaatcc gctgttcatt attaatctca gctcttcccg taaagccaat
     1981 gttgaaacta ttcgtaaatg tacctcgttt tgcgtgtacc ttgcttatca cgtgatatta
     2041 catgacctgg acagagttct gcgcgaaagt cataacgtaa atcccgggcg gtaggtgcgt
     2101 cccgggcgga aggtagtttt ctcgtccacc ccaacgcgtt tatcaacctc aactttcaac
     2161 aaccatcatg ccaccaaaag cgcgtaaaac aaagcgagat ttgattgagc aagagggcag
     2221 gatccaatgc gcgattcaag acattaaaaa tggaaaattt caaaaaattg cgcccgcagc
     2281 gcgtgcatac aaaattcatc ccaatacaag tgtaatgcta gtggaggtca acacatcaat
     2341 gcctattttg gtttagtcgt ccaggcggtg agcacaaaat ttgtgtcgtt tgacaagatg
     2401 gttcatttag gcaactggtc agatcagccc cacttgtagc agtagcggcg gcgctcgaag
     2461 tgtgactctt attagcagac aggaacgagg acattattat catctgctgc ttggtgcacg
     2521 ataacttggt gcgtttgtca agcaaggtaa gtggacgacc cggtcatacc ttcttaagtt
     2581 cgcccttcct ccctttattt cagattcaat ctgacttacc tattctaccc aagcatccaa
     2641 atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac
     2701 agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat
     2761 gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat
     2821 cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt
     2881 ggggagttca gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg
     2941 caagacctgc ctgaaaccga actgcccgct gttctccagc cggtcgcgga ggccatggat
     3001 gcgattgctg cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga
     3061 atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat
     3121 cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag
     3181 ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcatgc ggatttcggc
     3241 tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg
     3301 atgttcgggg attcccaata cgaggtcgcc aacatcctct tctggaggcc gtggttggct
     3361 tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg
     3421 cgcctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac
     3481 ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga
     3541 gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc
     3601 tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag
     3661 gaatagagta gatgccgacc gatccccact accgcattaa gacctcagcg cggccgcaaa
     3721 tttaaataaa atgaagtgaa gttcctatac tttctagaga ataggaactt ctatagtgag
     3781 tcgaataagg gcgacacaaa atttattcta aatgcataat aaatactgat aacatcttat
     3841 agtttgtatt atattttgta ttatcgttga catgtataat tttgatatca aaaactgatt
     3901 ttccctttat tattttcgag atttattttc ttaattctct ttaacaaact agaaatattg
     3961 tatatacaaa aaatcataaa taatagatga atagtttaat tataggtgtt catcaatcga
     4021 aaaagcaacg tatcttattt aaagtgcgtt gcttttttct catttataag gttaaataat
     4081 tctcatatat caagcaaagt gacaggcgcc cttaaatatt ctgacaaatg ctctttccct
     4141 aaactccccc cataaaaaaa cccgccgaag cgggttttta cgttatttgc ggattaacga
     4201 ttactcgtta tcagaaccgc ccagggggcc cgagcttaag actggccgtc gttttacaac
     4261 acagaaagag tttgtagaaa cgcaaaaagg ccatccgtca ggggccttct gcttagtttg
     4321 atgcctggca gttccctact ctcgccttcc gcttcctcgc tcactgactc gctgcgctcg
     4381 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     4441 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     4501 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     4561 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     4621 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     4681 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     4741 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     4801 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     4861 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     4921 gctacagagt tcttgaagtg gtgggctaac tacggctaca ctagaagaac agtatttggt
     4981 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     5041 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     5101 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     5161 gacgcgcgcg taactcacgt taagggattt tggtcatgag cttgcgccgt cccgtcaagt
     5221 cagcgtaatg ctctgcttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     5281 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     5341 gcttaccatc tggccccagc gctgcgatga taccgcgaga accacgctca ccggctccgg
     5401 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     5461 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     5521 ttaatagttt gcgcaacgtt gttgccatcg ctacaggcat cgtggtgtca cgctcgtcgt
     5581 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     5641 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     5701 ccgcagtgtt atcactcatg gttatggcag cgctacataa ttctcttact gtcatgccat
     5761 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     5821 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     5881 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     5941 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     6001 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     6061 agggaataag ggcgacacgg aaatgttgaa tactcatatt cttccttttt caatattatt
     6121 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     6181 ataaacaaat aggggtcagt gttacaacca attaaccaat tctgaacatt atcgcgagcc
     6241 catttatacc tgaatatggc tcataacacc ccttgtttgc ctggcggcag tagcgcggtg
     6301 gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg
     6361 gggactcccc atgcgagagt agggaactgc caggcatcaa ataaaacgaa aggctcagtc
     6421 gaaagactgg gcctttcgcc cgggctaatt atggggtgtc gcccttattc gactctatag
     6481 tgaagttcct attctctaga aagtatagga acttctgaag tggggattta aatgcggccg
     6541 cgctgagggt ttaatcgacg aagcagctga cggccagtgc caagcttaac gcgtaccggg
     6601 cccagtatat gttccgcaga tgactggagc tctgccatac gtgccctctc aagcaccatt
     6661 tgttccatct acagagacta gtcaccaact agtctatcaa gactcacagg gtacattgct
     6721 gagaccaact gaccagaggc agggtagcgg attgacggct ccatctcctt cacttacaag
     6781 gtctattgaa agccctttag catcaccaag cggagaatag attgttaagc ttattttttg
     6841 tatactgttt tgtgatagca cgaagttttt ccacggtatc ttgttaaaaa tatatatttg
     6901 tggcgggctt acctacatca aattaataag agactaatta taaactaaac acacaagcaa
     6961 gctactttag ggtaaaagtt tataaatgct tttgacgtat aaacgttgct tgtatttatt
     7021 attacaatta aaggtggata gaaaacctag agactagtta gaaactaatc tcaggtttgc
     7081 gttaaactaa atcagagccc gagaggttaa cagaacctag aaggggacta gatatccggg
     7141 tagggaaaca aaaaaaaaaa caagacagcc acatattagg gagactagtt agaagctagt
     7201 tccaggacta ggaaaataaa agacaatgat accacagtct agttgacaac tagatagatt
     7261 ctagattgag gccaaagtct ctgagatcca ggttagttgc aactaatact agttagtatc
     7321 tagtctccta taactctgaa gctagaataa cttactacta ttatcctcac cactgttcag
     7381 ctgcgcaaac ggagtgattg caaggtgttc agagactagt tattgactag tcagtgacta
     7441 gcaataacta acaaggtatt aacctaccat gtctgccatc accctgcact tcctcgggct
     7501 cagcagcctt ttcctcctca ttttcatgct cattttcctt gtttaagact gtgactagtc
     7561 aaagactagt ccagaaccac aaaggagaaa tgtcttacca ctttcttcat tgcttgtctc
     7621 ttttgcatta tccatgtctg caactagtta gagtctagtt agtgactagt ccgacgagga
     7681 cttgcttgtc tccggattgt tggaggaact ctccagggcc tcaagatcca caacagagcc
     7741 ttctagaaga ctggtcaata actagttggt ctttgtctga gtctgactta cgaggttgca
     7801 tactcgctcc ctttgcctcg tcaatcgatg agaaaaagcg ccaaaactcg caatatggct
     7861 ttgaaccaca cggtgctgag actagttaga atctagtccc aaactagctt ggatagctta
     7921 cctttgccct ttgcgttgcg acaggtcttg cagggtatgg ttcctttctc accagctgat
     7981 ttagctgcct tgctaccctc acggcggatc tgcataaaga gtggctagag gttataaatt
     8041 agcactgatc ctaggtacgg ggctgaatgt aacttgccyt tcctttctca tcgcgcggca
     8101 agacaggctt gctcaaattc ctaccagtca caggggtatg cacggcgtac ggaccacttg
     8161 aactagtcac agattagtta gcaactagtc tgcattgaat ggctgtactt acgggccctc
     8221 gccattgtcc tgatcatttc cagcttcacc ctcgttgctg caaagtagtt agtgactagt
     8281 caaggactag ttgaaatggg agaagaaact cacgaattct cgacaccctt agtattgtgg
     8341 tccttggact tggtgctgct atatattagc taatacacta gttagactca cagaaactta
     8401 cgcagctcgc ttgcgcttct tggtaggagt cggggttggg agaacagtgc cttcaaacaa
     8461 gccttcatac catgctactt gactagtcag ggactagtca ccaagtaatc tagataggac
     8521 ttgcctttgg cctccatcag ttccttcata gtgggaggtc cattgtgcaa tgtaaactcc
     8581 atgccgtggg agttcttatc cttcaagtgc ttgaccaata tgtttctgtt ggcagaggga
     8641 acctgtcaac tagttaataa ctagtcagaa actagtatag cagtagactc actgtacgct
     8701 tgaggcatcc cttcactcgg cagtagactt catatggatg gatatcaggc acgccattgt
     8761 cgtcctgtgg actagtcagt aactaggctt aaagctagtc gggtcggctt actatcttga
     8821 aatccggcag cgtaagctcc ccgtccttaa ctgcctcgag atagtgacag tactctgggg
     8881 actttcggag atcgttatcg cgaatgctcg gcatactaat cgttgactag tcttggacta
     8941 gtcccgagca aaaaggattg gaggaggagg aggaaggtga gagtgagaca aagagcgaaa
     9001 taagagcttc aaaggctatc tctaagcagt atgaaggtta agtatctagt tcttgactag
     9061 atttaaaaga gatttcgact agttatgtac ctggagtttg gatataggaa tgtgttgtgg
     9121 taacgaaatg taagggggag gaaagaaaaa gtcggtcaag aggtaactct aagtcggcca
     9181 ttcctttttg ggaggcgcta accataaacg gcatggtcga cttagagtta gctcagggaa
     9241 tttagggagt tatctgcgac caccgaggaa cggcggaatg ccaaagaatc ccgatggagc
     9301 tctagctggc ggttgacaac cccacctttt ggcgtttctg cggcgttgca ggcgggactg
     9361 gatacttcgt agaaccagaa aggcaaggca gaacgcgctc agcaagagtg ttggaagtga
     9421 tagcatgatg tgccttgtta actaggtcaa aatctgcagt atgcttgatg ttatccaaag
     9481 tgtgagagag gaaggtccaa acatacacga ttgggagagg gcctaggtat aagagttttt
     9541 gagtagaacg catgtgagcc cagccatctc gaggagatta aacacgggcc ggcatttgat
     9601 ggctatgtta gtaccccaat ggaaacggtg agagtccagt ggtcgcagat aactccctaa
     9661 attccctgag ctaactctaa gtcgaccatg ccgtttatgg ttagcgcctc ccaaaaagga
     9721 atggccgact tagagttacc tcttgaccga ctttttcttt cctccccctt acatttcgtt
     9781 accacaacac attcctatat ccaaactcca ggtacataac tagtcgaaat ctcttttaaa
     9841 tctagtcaag aactagatac ttaaccttca tactgcttag agatagcctt tgaagctctt
     9901 atttcgctct ttgtctcact ctcaccttcc tcctcctcct ccaatccttt ttgctcggga
     9961 ctagtccaag actagtcaac gattagtatg ccgagcattc gcgataacga tctccgaaag
    10021 tccccagagt actgtcacta tctcgaggca gttaaggacg gggagcttac gctgccggat
    10081 ttcaagatag taagccgacc cgactagctt taagcctagt tactgactag tccacaggac
    10141 gacaatggcg tgcctgatat ccatccatat gaagtctact gccgagtgaa gggatgcctc
    10201 aagcgtacag tgagtctact gctatactag tttctgacta gttattaact agttgacagg
    10261 ttccctctgc caacagaaac atattggtca agcacttgaa ggacaagaac tcccacggca
    10321 tggagtttac attgcacaat ggacctccca ctatgaagga actgatggag gccaaaggca
    10381 agtcctatct agattacttg gtgactagtc cctgactagt caagtagcat ggtatgaagg
    10441 cttgtttgaa ggcactgttc tcccaacccc gactcctacc aagaagcgca agcgagctgc
    10501 gtaagtttct gtgagtctaa ctagtgtatt agctaatata tagcagcacc aagtccaagg
    10561 accacaatac taagggtgtc gagaattcgt gagtttcttc tcccatttca actagtcctt
    10621 gactagtcac taactacttt gcagcaacga gggtgaagct ggaaatgatc aggacaatgg
    10681 cgagggcccg taagtacagc cattcaatgc agactagttg ctaactaatc tgtgactagt
    10741 tcaagtggtc cgtacgccgt gcatacccct gtgactggta ggaatttgag caagcctgtc
    10801 ttgccgcgcg atgagaaagg aarggcaagt tacattcagc cccgtaccta ggatcagtgc
    10861 taatttataa cctctagcca ctctttatgc agatccgccg tgagggtagc aaggcagcta
    10921 aatcagctgg tgagaaagga accataccct gcaagacctg tcgcaacgca aagggcaaag
    10981 gtaagctatc caagctagtt tgggactaga ttctaactag tctcagcacc gtgtggttca
    11041 aagccatatt gcgagttttg gcgctttttc tcatcgattg acgaggcaaa gggagcgagt
    11101 atgcaacctc gtaagtcaga ctcagacaaa gaccaactag ttattgacca gtcttctaga
    11161 aggctctgtt gtggatcttg aggccctgga gagttcctcc aacaatccgg agacaagcaa
    11221 gtcctcgtcg gactagtcac taactagact ctaactagtt gcagacatgg ataatgcaaa
    11281 agagacaagc aatgaagaaa gtggtaagac atttctcctt tgtggttctg gactagtctt
    11341 tgactagtca cagtcttaaa caaggaaaat gagcatgaaa atgaggagga aaaggctgct
    11401 gagcccgagg aagtgcaggg tgatggcaga catggtaggt taataccttg ttagttattg
    11461 ctagtcactg actagtcaat aactagtctc tgaacacctt gcaatcactc cgtttgcgca
    11521 gctgaacagt ggtgaggata atagtagtaa gttattctag cttcagagtt ataggagact
    11581 agatactaac tagtattagt tgcaactaac ctggatctca gagactttgg cctcaatcta
    11641 gaatctatct agttgtcaac tagactgtgg tatcattgtc ttttattttc ctagtcctgg
    11701 aactagcttc taactagtct ccctaatatg tggctgtctt gttttttttt tttgtttccc
    11761 tacccggata tctagtcccc ttctaggttc tgttaacctc tcgggctctg atttagttta
    11821 acgcaaacct gagattagtt tctaactagt ctctaggttt tctatccacc tttaattgta
    11881 ataataaata caagcaacgt ttatacgtca aaagcattta taaactttta ccctaaagta
    11941 gcttgcttgt gtgtttagtt tataattagt ctcttattaa tttgatgtag gtaagcccgc
    12001 cacaaatata tatttttaac aagataccgt ggaaaaactt cgtgctatca caaaacagta
    12061 tacaaaaaat aagct
//
