LOCUS       sequence-483847-       12108 bp    DNA     circular SYN 19-AUG-2025
DEFINITION  Express mCherry in filamentous fungi.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12108)
  TITLE     A Toolbox of TEF1-Intron-Based Strong Promoter Reporter Systems for 
            Filamentous Fungi
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12108)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 19, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pGY208
FEATURES             Location/Qualifiers
     source          1..12108
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1144..1851
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKGGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(1286..1304)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1554..1573
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             2674..3699
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANILFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(3771..3804)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(4274..4290)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4389..4406)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4560..5148)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4640..4659)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5270..6061,6062..6130))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5893..5912
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6131..6222)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6264..6283
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6289..6308
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    6515..6548
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     rep_origin      6861..12108
                     /label=AMA1
                     /note="replication origin from Aspergillus nidulans 
                     (Aleksenko and Clutterbuck, 1996)"
ORIGIN
        1 taacaatcta ttctccgctt ggtgatgcta aagggctttc aatagacctt gtaagtgaag
       61 gagatggagc cgtcaatccg ctaccctgcc tctggtcagt tggtctcagc aatgtaccct
      121 gtgagtcttg atagactagt tggtgactag tctctgtaga tggaacaaat ggtgcttgag
      181 agggcacgta tggcagagct ccagtcatct gcggaacata tactgggccc gggaagatct
      241 catggtcata gctgtttccg ctgagggttt aatcgttgac ctcacaggga tttccaccaa
      301 aaagtggacc gggcgggcgc cactcggccg tgccacagca gcctgagagc ggacaaataa
      361 caacagccgc ctgccgcggg gttcggttgc aaacatgacc aacaggccag gccatcatca
      421 acccaccgct gcgttgatgc ccaggatttc agtccaataa tccacaattt accaacggat
      481 agagctaggt gaactagata gacaggaggg ccagagggag gggaccgaga tgaaaaattt
      541 tcgatgaaag agtggtcaag gtggggtcgt agttcggcgc tccgagggcg aggaaccaag
      601 gaaaggcgag aaaaggacag gctgatcgcg ctgcgttgct gggctgcaag cgtgtccagt
      661 tgagtctgga aaaggctccg ccgtgaagat tctgcgttgg tcccgcacct gcgcggtggg
      721 ggcattaccc ctccatgtcc aatgatttca agtcaaagcc aagggttgaa gcccgcccgc
      781 ttagtcgcct tctcgcttga cccctccata taagtatttc ccctcctccc cctcccacaa
      841 atttttcctt tccctttcct ccctcgtccg cttcagtacg tatatcttcc ccccctctct
      901 cttccttctc actcttctct ccttctttct tgattcatcc tctctctaac tgacttcttt
      961 gctcagcacc tctacgcgtt ctggccgtag tatctgagca atttttctac agactttttc
     1021 tatctaattc caaaaaagaa cttcgagttc attcacaacc gtcatcatgg ggtgagtcaa
     1081 gacaacgatc atcatctgac aaaggaagga catgggctaa cggatatctt ctacaacagt
     1141 aaggtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag
     1201 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
     1261 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
     1321 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac
     1381 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc
     1441 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
     1501 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta
     1561 atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc
     1621 gccctgaagg gcgagatcaa gcagaggctg aagctgaagg gcggcggcca ctacgacgct
     1681 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc
     1741 aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa
     1801 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagta agcggacatt
     1861 cgatttatgc cgttatgact tccttaaaaa agcctttacg aatgaaagaa atggaattag
     1921 acttgttatg tagttgattc tacaatggat tatgattcct gaacttcaaa tccgctgttc
     1981 attattaatc tcagctcttc ccgtaaagcc aatgttgaaa ctattcgtaa atgtacctcg
     2041 ttttgcgtgt accttgctta tcacgtgata ttacatgacc tggacagagt tctgcgcgaa
     2101 agtcataacg taaatcccgg gcggtaggtg cgtcccgggc ggaaggtagt tttctcgtcc
     2161 accccaacgc gtttatcaac ctcaactttc aacaaccatc atgccaccaa aagcgcgtaa
     2221 aacaaagcga gatttgattg agcaagaggg caggatccaa tgcgcgattc aagacattaa
     2281 aaatggaaaa tttcaaaaaa ttgcgcccgc agcgcgtgca tacaaaattc atcccaatac
     2341 aagtgtaatg ctagtggagg tcaacacatc aatgcctatt ttggtttagt cgtccaggcg
     2401 gtgagcacaa aatttgtgtc gtttgacaag atggttcatt taggcaactg gtcagatcag
     2461 ccccacttgt agcagtagcg gcggcgctcg aagtgtgact cttattagca gacaggaacg
     2521 aggacattat tatcatctgc tgcttggtgc acgataactt ggtgcgtttg tcaagcaagg
     2581 taagtggacg acccggtcat accttcttaa gttcgccctt cctcccttta tttcagattc
     2641 aatctgactt acctattcta cccaagcatc caaatgaaaa agcctgaact caccgcgacg
     2701 tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg
     2761 gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg
     2821 gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg
     2881 gccgcgctcc cgattccgga agtgcttgac attggggagt tcagcgagag cctgacctat
     2941 tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc
     3001 gctgttctcc agccggtcgc ggaggccatg gatgcgattg ctgcggccga tcttagccag
     3061 acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat
     3121 ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc
     3181 gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc
     3241 gaagtccggc acctcgtgca tgcggatttc ggctccaaca atgtcctgac ggacaatggc
     3301 cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc
     3361 gccaacatcc tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc
     3421 gagcggaggc atccggagct tgcaggatcg ccgcgcctcc gggcgtatat gctccgcatt
     3481 ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg
     3541 cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc
     3601 gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga
     3661 aaccgacgcc ccagcactcg tccgagggca aaggaataga gtagatgccg accgatcccc
     3721 actaccgcat taagacctca gcgcggccgc aaatttaaat aaaatgaagt gaagttccta
     3781 tactttctag agaataggaa cttctatagt gagtcgaata agggcgacac aaaatttatt
     3841 ctaaatgcat aataaatact gataacatct tatagtttgt attatatttt gtattatcgt
     3901 tgacatgtat aattttgata tcaaaaactg attttccctt tattattttc gagatttatt
     3961 ttcttaattc tctttaacaa actagaaata ttgtatatac aaaaaatcat aaataataga
     4021 tgaatagttt aattataggt gttcatcaat cgaaaaagca acgtatctta tttaaagtgc
     4081 gttgcttttt tctcatttat aaggttaaat aattctcata tatcaagcaa agtgacaggc
     4141 gcccttaaat attctgacaa atgctctttc cctaaactcc ccccataaaa aaacccgccg
     4201 aagcgggttt ttacgttatt tgcggattaa cgattactcg ttatcagaac cgcccagggg
     4261 gcccgagctt aagactggcc gtcgttttac aacacagaaa gagtttgtag aaacgcaaaa
     4321 aggccatccg tcaggggcct tctgcttagt ttgatgcctg gcagttccct actctcgcct
     4381 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     4441 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     4501 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     4561 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     4621 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     4681 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     4741 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     4801 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     4861 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     4921 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtgggct
     4981 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     5041 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     5101 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     5161 atcttttcta cggggtctga cgctcagtgg aacgacgcgc gcgtaactca cgttaaggga
     5221 ttttggtcat gagcttgcgc cgtcccgtca agtcagcgta atgctctgct taccaatgct
     5281 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     5341 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agcgctgcga
     5401 tgataccgcg agaaccacgc tcaccggctc cggatttatc agcaataaac cagccagccg
     5461 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     5521 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     5581 tcgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     5641 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     5701 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     5761 cagcgctaca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     5821 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     5881 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     5941 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     6001 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     6061 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     6121 gaatactcat attcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     6181 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtc agtgttacaa
     6241 ccaattaacc aattctgaac attatcgcga gcccatttat acctgaatat ggctcataac
     6301 accccttgtt tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat gccgaactca
     6361 gaagtgaaac gccgtagcgc cgatggtagt gtggggactc cccatgcgag agtagggaac
     6421 tgccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gcccgggcta
     6481 attatggggt gtcgccctta ttcgactcta tagtgaagtt cctattctct agaaagtata
     6541 ggaacttctg aagtggggat ttaaatgcgg ccgcgctgag ggtttaatcg acgaagcagc
     6601 tgacggccag tgccaagctt aacgcgtacc gggcccagta tatgttccgc agatgactgg
     6661 agctctgcca tacgtgccct ctcaagcacc atttgttcca tctacagaga ctagtcacca
     6721 actagtctat caagactcac agggtacatt gctgagacca actgaccaga ggcagggtag
     6781 cggattgacg gctccatctc cttcacttac aaggtctatt gaaagccctt tagcatcacc
     6841 aagcggagaa tagattgtta agcttatttt ttgtatactg ttttgtgata gcacgaagtt
     6901 tttccacggt atcttgttaa aaatatatat ttgtggcggg cttacctaca tcaaattaat
     6961 aagagactaa ttataaacta aacacacaag caagctactt tagggtaaaa gtttataaat
     7021 gcttttgacg tataaacgtt gcttgtattt attattacaa ttaaaggtgg atagaaaacc
     7081 tagagactag ttagaaacta atctcaggtt tgcgttaaac taaatcagag cccgagaggt
     7141 taacagaacc tagaagggga ctagatatcc gggtagggaa acaaaaaaaa aaacaagaca
     7201 gccacatatt agggagacta gttagaagct agttccagga ctaggaaaat aaaagacaat
     7261 gataccacag tctagttgac aactagatag attctagatt gaggccaaag tctctgagat
     7321 ccaggttagt tgcaactaat actagttagt atctagtctc ctataactct gaagctagaa
     7381 taacttacta ctattatcct caccactgtt cagctgcgca aacggagtga ttgcaaggtg
     7441 ttcagagact agttattgac tagtcagtga ctagcaataa ctaacaaggt attaacctac
     7501 catgtctgcc atcaccctgc acttcctcgg gctcagcagc cttttcctcc tcattttcat
     7561 gctcattttc cttgtttaag actgtgacta gtcaaagact agtccagaac cacaaaggag
     7621 aaatgtctta ccactttctt cattgcttgt ctcttttgca ttatccatgt ctgcaactag
     7681 ttagagtcta gttagtgact agtccgacga ggacttgctt gtctccggat tgttggagga
     7741 actctccagg gcctcaagat ccacaacaga gccttctaga agactggtca ataactagtt
     7801 ggtctttgtc tgagtctgac ttacgaggtt gcatactcgc tccctttgcc tcgtcaatcg
     7861 atgagaaaaa gcgccaaaac tcgcaatatg gctttgaacc acacggtgct gagactagtt
     7921 agaatctagt cccaaactag cttggatagc ttacctttgc cctttgcgtt gcgacaggtc
     7981 ttgcagggta tggttccttt ctcaccagct gatttagctg ccttgctacc ctcacggcgg
     8041 atctgcataa agagtggcta gaggttataa attagcactg atcctaggta cggggctgaa
     8101 tgtaacttgc ccttcctttc tcatcgcgcg gcaagacagg cttgctcaaa ttcctaccag
     8161 tcacaggggt atgcacggcg tacggaccac ttgaactagt cacagattag ttagcaacta
     8221 gtctgcattg aatggctgta cttacgggcc ctcgccattg tcctgatcat ttccagcttc
     8281 accctcgttg ctgcaaagta gttagtgact agtcaaggac tagttgaaat gggagaagaa
     8341 actcacgaat tctcgacacc cttagtattg tggtccttgg acttggtgct gctatatatt
     8401 agctaataca ctagttagac tcacagaaac ttacgcagct cgcttgcgct tcttggtagg
     8461 agtcggggtt gggagaacag tgccttcaaa caagccttca taccatgcta cttgactagt
     8521 cagggactag tcaccaagta atctagatag gacttgcctt tggcctccat cagttccttc
     8581 atagtgggag gtccattgtg caatgtaaac tccatgccgt gggagttctt atccttcaag
     8641 tgcttgacca atatgtttct gttggcagag ggaacctgtc aactagttaa taactagtca
     8701 gaaactagta tagcagtaga ctcactgtac gcttgaggca tcccttcact cggcagtaga
     8761 cttcatatgg atggatatca ggcacgccat tgtcgtcctg tggactagtc agtaactagg
     8821 cttaaagcta gtcgggtcgg cttactatct tgaaatccgg cagcgtaagc tccccgtcct
     8881 taactgcctc gagatagtga cagtactctg gggactttcg gagatcgtta tcgcgaatgc
     8941 tcggcatact aatcgttgac tagtcttgga ctagtcccga gcaaaaagga ttggaggagg
     9001 aggaggaagg tgagagtgag acaaagagcg aaataagagc ttcaaaggct atctctaagc
     9061 agtatgaagg ttaagtatct agttcttgac tagatttaaa agagatttcg actagttatg
     9121 tacctggagt ttggatatag gaatgtgttg tggtaacgaa atgtaagggg gaggaaagaa
     9181 aaagtcggtc aagaggtaac tctaagtcgg ccattccttt ttgggaggcg ctaaccataa
     9241 acggcatggt cgacttagag ttagctcagg gaatttaggg agttatctgc gaccaccgag
     9301 gaacggcgga atgccaaaga atcccgatgg agctctagct ggcggttgac aaccccacct
     9361 tttggcgttt ctgcggcgtt gcaggcggga ctggatactt cgtagaacca gaaaggcaag
     9421 gcagaacgcg ctcagcaaga gtgttggaag tgatagcatg atgtgccttg ttaactaggt
     9481 caaaatctgc agtatgcttg atgttatcca aagtgtgaga gaggaaggtc caaacataca
     9541 cgattgggag agggcctagg tataagagtt tttgagtaga acgcatgtga gcccagccat
     9601 ctcgaggaga ttaaacacgg gccggcattt gatggctatg ttagtacccc aatggaaacg
     9661 gtgagagtcc agtggtcgca gataactccc taaattccct gagctaactc taagtcgacc
     9721 atgccgttta tggttagcgc ctcccaaaaa ggaatggccg acttagagtt acctcttgac
     9781 cgactttttc tttcctcccc cttacatttc gttaccacaa cacattccta tatccaaact
     9841 ccaggtacat aactagtcga aatctctttt aaatctagtc aagaactaga tacttaacct
     9901 tcatactgct tagagatagc ctttgaagct cttatttcgc tctttgtctc actctcacct
     9961 tcctcctcct cctccaatcc tttttgctcg ggactagtcc aagactagtc aacgattagt
    10021 atgccgagca ttcgcgataa cgatctccga aagtccccag agtactgtca ctatctcgag
    10081 gcagttaagg acggggagct tacgctgccg gatttcaaga tagtaagccg acccgactag
    10141 ctttaagcct agttactgac tagtccacag gacgacaatg gcgtgcctga tatccatcca
    10201 tatgaagtct actgccgagt gaagggatgc ctcaagcgta cagtgagtct actgctatac
    10261 tagtttctga ctagttatta actagttgac aggttccctc tgccaacaga aacatattgg
    10321 tcaagcactt gaaggacaag aactcccacg gcatggagtt tacattgcac aatggacctc
    10381 ccactatgaa ggaactgatg gaggccaaag gcaagtccta tctagattac ttggtgacta
    10441 gtccctgact agtcaagtag catggtatga aggcttgttt gaaggcactg ttctcccaac
    10501 cccgactcct accaagaagc gcaagcgagc tgcgtaagtt tctgtgagtc taactagtgt
    10561 attagctaat atatagcagc accaagtcca aggaccacaa tactaagggt gtcgagaatt
    10621 cgtgagtttc ttctcccatt tcaactagtc cttgactagt cactaactac tttgcagcaa
    10681 cgagggtgaa gctggaaatg atcaggacaa tggcgagggc ccgtaagtac agccattcaa
    10741 tgcagactag ttgctaacta atctgtgact agttcaagtg gtccgtacgc cgtgcatacc
    10801 cctgtgactg gtaggaattt gagcaagcct gtcttgccgc gcgatgagaa aggaaaggca
    10861 agttacattc agccccgtac ctaggatcag tgctaattta taacctctag ccactcttta
    10921 tgcagatccg ccgtgagggt agcaaggcag ctaaatcagc tggtgagaaa ggaaccatac
    10981 cctgcaagac ctgtcgcaac gcaaagggca aaggtaagct atccaagcta gtttgggact
    11041 agattctaac tagtctcagc accgtgtggt tcaaagccat attgcgagtt ttggcgcttt
    11101 ttctcatcga ttgacgaggc aaagggagcg agtatgcaac ctcgtaagtc agactcagac
    11161 aaagaccaac tagttattga ccagtcttct agaaggctct gttgtggatc ttgaggccct
    11221 ggagagttcc tccaacaatc cggagacaag caagtcctcg tcggactagt cactaactag
    11281 actctaacta gttgcagaca tggataatgc aaaagagaca agcaatgaag aaagtggtaa
    11341 gacatttctc ctttgtggtt ctggactagt ctttgactag tcacagtctt aaacaaggaa
    11401 aatgagcatg aaaatgagga ggaaaaggct gctgagcccg aggaagtgca gggtgatggc
    11461 agacatggta ggttaatacc ttgttagtta ttgctagtca ctgactagtc aataactagt
    11521 ctctgaacac cttgcaatca ctccgtttgc gcagctgaac agtggtgagg ataatagtag
    11581 taagttattc tagcttcaga gttataggag actagatact aactagtatt agttgcaact
    11641 aacctggatc tcagagactt tggcctcaat ctagaatcta tctagttgtc aactagactg
    11701 tggtatcatt gtcttttatt ttcctagtcc tggaactagc ttctaactag tctccctaat
    11761 atgtggctgt cttgtttttt ttttttgttt ccctacccgg atatctagtc cccttctagg
    11821 ttctgttaac ctctcgggct ctgatttagt ttaacgcaaa cctgagatta gtttctaact
    11881 agtctctagg ttttctatcc acctttaatt gtaataataa atacaagcaa cgtttatacg
    11941 tcaaaagcat ttataaactt ttaccctaaa gtagcttgct tgtgtgttta gtttataatt
    12001 agtctcttat taatttgatg taggtaagcc cgccacaaat atatattttt aacaagatac
    12061 cgtggaaaaa cttcgtgcta tcacaaaaca gtatacaaaa aataagct
//
