LOCUS       sequence-483853-       13209 bp    DNA     circular SYN 19-AUG-2025
DEFINITION  Express GUS in filamentous fungi.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13209)
  TITLE     A Toolbox of TEF1-Intron-Based Strong Promoter Reporter Systems for 
            Filamentous Fungi
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13209)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 19, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pGY203
FEATURES             Location/Qualifiers
     source          1..13209
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1144..2952
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="LRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAIA
                     VPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEVM
                     EHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHDF
                     FNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQV
                     VATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQFL
                     INHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDWA
                     DEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARDK
                     NHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISDL
                     FDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYT
                     DMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRKP
                     KSAAFLLQKRWTGMNFGEKPQQGGKQ"
     CDS             3775..4800
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANILFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(4872..4905)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(5375..5391)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5490..5507)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5661..6249)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5741..5760)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6371..7162,7163..7231))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6994..7013
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7232..7323)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7365..7384
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     7390..7409
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    7616..7649
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     rep_origin      7962..13209
                     /label=AMA1
                     /note="replication origin from Aspergillus nidulans 
                     (Aleksenko and Clutterbuck, 1996)"
ORIGIN
        1 taacaatcta ttctccgctt ggtgatgcta aagggctttc aatagacctt gtaagtgaag
       61 gagatggagc cgtcaatccg ctaccctgcc tctggtcagt tggtctcagc aatgtaccct
      121 gtgagtcttg atagactagt tggtgactag tctctgtaga tggaacaaat ggtgcttgag
      181 agggcacgta tggcagagct ccagtcatct gcggaacata tactgggccc gggaagatct
      241 catggtcata gctgtttccg ctgagggttt aatcgttgac ctcacaggga tttccaccaa
      301 aaagtggacc gggcgggcgc cactcggccg tgccacagca gcctgagagc ggacaaataa
      361 caacagccgc ctgccgcggg gttcggttgc aaacatgacc aacaggccag gccatcatca
      421 acccaccgct gcgttgatgc ccaggatttc agtccaataa tccacaattt accaacggat
      481 agagctaggt gaactagata gacaggaggg ccagagggag gggaccgaga tgaaaaattt
      541 tcgatgaaag agtggtcaag gtggggtcgt agttcggcgc tccgagggcg aggaaccaag
      601 gaaaggcgag aaaaggacag gctgatcgcg ctgcgttgct gggctgcaag cgtgtccagt
      661 tgagtctgga aaaggctccg ccgtgaagat tctgcgttgg tcccgcacct gcgcggtggg
      721 ggcattaccc ctccatgtcc aatgatttca agtcaaagcc aagggttgaa gcccgcccgc
      781 ttagtcgcct tctcgcttga cccctccata taagtatttc ccctcctccc cctcccacaa
      841 atttttcctt tccctttcct ccctcgtccg cttcagtacg tatatcttcc ccccctctct
      901 cttccttctc actcttctct ccttctttct tgattcatcc tctctctaac tgacttcttt
      961 gctcagcacc tctacgcgtt ctggccgtag tatctgagca atttttctac agactttttc
     1021 tatctaattc caaaaaagaa cttcgagttc attcacaacc gtcatcatgg ggtgagtcaa
     1081 gacaacgatc atcatctgac aaaggaagga catgggctaa cggatatctt ctacaacagt
     1141 aagttacgtc ctgtagaaac cccaacccgt gaaatcaaaa aactcgacgg cctgtgggca
     1201 ttcagtctgg atcgcgaaaa ctgtggaatt gatcagcgtt ggtgggaaag cgcgttacaa
     1261 gaaagccggg caattgctgt gccaggcagt tttaacgatc agttcgccga tgcagatatt
     1321 cgtaattatg cgggcaacgt ctggtatcag cgcgaagtct ttataccgaa aggttgggca
     1381 ggccagcgta tcgtgctgcg tttcgatgcg gtcactcatt acggcaaagt gtgggtcaat
     1441 aatcaggaag tgatggagca tcagggcggc tatacgccat ttgaagccga tgtcacgccg
     1501 tatgttattg ccgggaaaag tgtacgtatc accgtttgtg tgaacaacga actgaactgg
     1561 cagactatcc cgccgggaat ggtgattacc gacgaaaacg gcaagaaaaa gcagtcttac
     1621 ttccatgatt tctttaacta tgccggaatc catcgcagcg taatgctcta caccacgccg
     1681 aacacctggg tggacgatat caccgtggtg acgcatgtcg cgcaagactg taaccacgcg
     1741 tctgttgact ggcaggtggt ggccaatggt gatgtcagcg ttgaactgcg tgatgcggat
     1801 caacaggtgg ttgcaactgg acaaggcact agcgggactt tgcaagtggt gaatccgcac
     1861 ctctggcaac cgggtgaagg ttatctctat gaactgtgcg tcacagccaa aagccagaca
     1921 gagtgtgata tctacccgct tcgcgtcggc atccggtcag tggcagtgaa gggcgaacag
     1981 ttcctgatta accacaaacc gttctacttt actggctttg gtcgtcatga agatgcggac
     2041 ttgcgtggca aaggattcga taacgtgctg atggtgcacg accacgcatt aatggactgg
     2101 attggggcca actcctaccg tacctcgcat tacccttacg ctgaagagat gctcgactgg
     2161 gcagatgaac atggcatcgt ggtgattgat gaaactgctg ctgtcggctt taacctctct
     2221 ttaggcattg gtttcgaagc gggcaacaag ccgaaagaac tgtacagcga agaggcagtc
     2281 aacggggaaa ctcagcaagc gcacttacag gcgattaaag agctgatagc gcgtgacaaa
     2341 aaccacccaa gcgtggtgat gtggagtatt gccaacgaac cggatacccg tccgcaaggt
     2401 gcacgggaat atttcgcgcc actggcggaa gcaacgcgta aactcgaccc gacgcgtccg
     2461 atcacctgcg tcaatgtaat gttctgcgac gctcacaccg ataccatcag cgatctcttt
     2521 gatgtgctgt gcctgaaccg ttattacgga tggtatgtcc aaagcggcga tttggaaacg
     2581 gcagagaagg tactggaaaa agaacttctg gcctggcagg agaaactgca tcagccgatt
     2641 atcatcaccg aatacggcgt ggatacgtta gccgggctgc actcaatgta caccgacatg
     2701 tggagtgaag agtatcagtg tgcatggctg gatatgtatc accgcgtctt tgatcgcgtc
     2761 agcgccgtcg tcggtgaaca ggtatggaat ttcgccgatt ttgcgacctc gcaaggcata
     2821 ttgcgcgttg gcggtaacaa gaaagggatc ttcactcgcg accgcaaacc gaagtcggcg
     2881 gcttttctgc tgcaaaaacg ctggactggc atgaacttcg gtgaaaaacc gcagcaggga
     2941 ggcaaacaat gagcggacat tcgatttatg ccgttatgac ttccttaaaa aagcctttac
     3001 gaatgaaaga aatggaatta gacttgttat gtagttgatt ctacaatgga ttatgattcc
     3061 tgaacttcaa atccgctgtt cattattaat ctcagctctt cccgtaaagc caatgttgaa
     3121 actattcgta aatgtacctc gttttgcgtg taccttgctt atcacgtgat attacatgac
     3181 ctggacagag ttctgcgcga aagtcataac gtaaatcccg ggcggtaggt gcgtcccggg
     3241 cggaaggtag ttttctcgtc caccccaacg cgtttatcaa cctcaacttt caacaaccat
     3301 catgccacca aaagcgcgta aaacaaagcg agatttgatt gagcaagagg gcaggatcca
     3361 atgcgcgatt caagacatta aaaatggaaa atttcaaaaa attgcgcccg cagcgcgtgc
     3421 atacaaaatt catcccaata caagtgtaat gctagtggag gtcaacacat caatgcctat
     3481 tttggtttag tcgtccaggc ggtgagcaca aaatttgtgt cgtttgacaa gatggttcat
     3541 ttaggcaact ggtcagatca gccccacttg tagcagtagc ggcggcgctc gaagtgtgac
     3601 tcttattagc agacaggaac gaggacatta ttatcatctg ctgcttggtg cacgataact
     3661 tggtgcgttt gtcaagcaag gtaagtggac gacccggtca taccttctta agttcgccct
     3721 tcctcccttt atttcagatt caatctgact tacctattct acccaagcat ccaaatgaaa
     3781 aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc
     3841 tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga
     3901 gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat
     3961 gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggag
     4021 ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac
     4081 ctgcctgaaa ccgaactgcc cgctgttctc cagccggtcg cggaggccat ggatgcgatt
     4141 gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca aggaatcggt
     4201 caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt gtatcactgg
     4261 caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg
     4321 ctttgggccg aggactgccc cgaagtccgg cacctcgtgc atgcggattt cggctccaac
     4381 aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga ggcgatgttc
     4441 ggggattccc aatacgaggt cgccaacatc ctcttctgga ggccgtggtt ggcttgtatg
     4501 gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc gccgcgcctc
     4561 cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt tgacggcaat
     4621 ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg
     4681 actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga tggctgtgta
     4741 gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc aaaggaatag
     4801 agtagatgcc gaccgatccc cactaccgca ttaagacctc agcgcggccg caaatttaaa
     4861 taaaatgaag tgaagttcct atactttcta gagaatagga acttctatag tgagtcgaat
     4921 aagggcgaca caaaatttat tctaaatgca taataaatac tgataacatc ttatagtttg
     4981 tattatattt tgtattatcg ttgacatgta taattttgat atcaaaaact gattttccct
     5041 ttattatttt cgagatttat tttcttaatt ctctttaaca aactagaaat attgtatata
     5101 caaaaaatca taaataatag atgaatagtt taattatagg tgttcatcaa tcgaaaaagc
     5161 aacgtatctt atttaaagtg cgttgctttt ttctcattta taaggttaaa taattctcat
     5221 atatcaagca aagtgacagg cgcccttaaa tattctgaca aatgctcttt ccctaaactc
     5281 cccccataaa aaaacccgcc gaagcgggtt tttacgttat ttgcggatta acgattactc
     5341 gttatcagaa ccgcccaggg ggcccgagct taagactggc cgtcgtttta caacacagaa
     5401 agagtttgta gaaacgcaaa aaggccatcc gtcaggggcc ttctgcttag tttgatgcct
     5461 ggcagttccc tactctcgcc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     5521 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     5581 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     5641 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     5701 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     5761 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     5821 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     5881 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     5941 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     6001 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     6061 gagttcttga agtggtgggc taactacggc tacactagaa gaacagtatt tggtatctgc
     6121 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     6181 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     6241 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgacgcg
     6301 cgcgtaactc acgttaaggg attttggtca tgagcttgcg ccgtcccgtc aagtcagcgt
     6361 aatgctctgc ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     6421 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     6481 catctggccc cagcgctgcg atgataccgc gagaaccacg ctcaccggct ccggatttat
     6541 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     6601 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     6661 gtttgcgcaa cgttgttgcc atcgctacag gcatcgtggt gtcacgctcg tcgtttggta
     6721 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     6781 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     6841 tgttatcact catggttatg gcagcgctac ataattctct tactgtcatg ccatccgtaa
     6901 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     6961 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     7021 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     7081 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     7141 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     7201 taagggcgac acggaaatgt tgaatactca tattcttcct ttttcaatat tattgaagca
     7261 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     7321 aaataggggt cagtgttaca accaattaac caattctgaa cattatcgcg agcccattta
     7381 tacctgaata tggctcataa caccccttgt ttgcctggcg gcagtagcgc ggtggtccca
     7441 cctgacccca tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag tgtggggact
     7501 ccccatgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     7561 ctgggccttt cgcccgggct aattatgggg tgtcgccctt attcgactct atagtgaagt
     7621 tcctattctc tagaaagtat aggaacttct gaagtgggga tttaaatgcg gccgcgctga
     7681 gggtttaatc gacgaagcag ctgacggcca gtgccaagct taacgcgtac cgggcccagt
     7741 atatgttccg cagatgactg gagctctgcc atacgtgccc tctcaagcac catttgttcc
     7801 atctacagag actagtcacc aactagtcta tcaagactca cagggtacat tgctgagacc
     7861 aactgaccag aggcagggta gcggattgac ggctccatct ccttcactta caaggtctat
     7921 tgaaagccct ttagcatcac caagcggaga atagattgtt aagcttattt tttgtatact
     7981 gttttgtgat agcacgaagt ttttccacgg tatcttgtta aaaatatata tttgtggcgg
     8041 gcttacctac atcaaattaa taagagacta attataaact aaacacacaa gcaagctact
     8101 ttagggtaaa agtttataaa tgcttttgac gtataaacgt tgcttgtatt tattattaca
     8161 attaaaggtg gatagaaaac ctagagacta gttagaaact aatctcaggt ttgcgttaaa
     8221 ctaaatcaga gcccgagagg ttaacagaac ctagaagggg actagatatc cgggtaggga
     8281 aacaaaaaaa aaaacaagac agccacatat tagggagact agttagaagc tagttccagg
     8341 actaggaaaa taaaagacaa tgataccaca gtctagttga caactagata gattctagat
     8401 tgaggccaaa gtctctgaga tccaggttag ttgcaactaa tactagttag tatctagtct
     8461 cctataactc tgaagctaga ataacttact actattatcc tcaccactgt tcagctgcgc
     8521 aaacggagtg attgcaaggt gttcagagac tagttattga ctagtcagtg actagcaata
     8581 actaacaagg tattaaccta ccatgtctgc catcaccctg cacttcctcg ggctcagcag
     8641 ccttttcctc ctcattttca tgctcatttt ccttgtttaa gactgtgact agtcaaagac
     8701 tagtccagaa ccacaaagga gaaatgtctt accactttct tcattgcttg tctcttttgc
     8761 attatccatg tctgcaacta gttagagtct agttagtgac tagtccgacg aggacttgct
     8821 tgtctccgga ttgttggagg aactctccag ggcctcaaga tccacaacag agccttctag
     8881 aagactggtc aataactagt tggtctttgt ctgagtctga cttacgaggt tgcatactcg
     8941 ctccctttgc ctcgtcaatc gatgagaaaa agcgccaaaa ctcgcaatat ggctttgaac
     9001 cacacggtgc tgagactagt tagaatctag tcccaaacta gcttggatag cttacctttg
     9061 ccctttgcgt tgcgacaggt cttgcagggt atggttcctt tctcaccagc tgatttagct
     9121 gccttgctac cctcacggcg gatctgcata aagagtggct agaggttata aattagcact
     9181 gatcctaggt acggggctga atgtaacttg ccyttccttt ctcatcgcgc ggcaagacag
     9241 gcttgctcaa attcctacca gtcacagggg tatgcacggc gtacggacca cttgaactag
     9301 tcacagatta gttagcaact agtctgcatt gaatggctgt acttacgggc cctcgccatt
     9361 gtcctgatca tttccagctt caccctcgtt gctgcaaagt agttagtgac tagtcaagga
     9421 ctagttgaaa tgggagaaga aactcacgaa ttctcgacac ccttagtatt gtggtccttg
     9481 gacttggtgc tgctatatat tagctaatac actagttaga ctcacagaaa cttacgcagc
     9541 tcgcttgcgc ttcttggtag gagtcggggt tgggagaaca gtgccttcaa acaagccttc
     9601 ataccatgct acttgactag tcagggacta gtcaccaagt aatctagata ggacttgcct
     9661 ttggcctcca tcagttcctt catagtggga ggtccattgt gcaatgtaaa ctccatgccg
     9721 tgggagttct tatccttcaa gtgcttgacc aatatgtttc tgttggcaga gggaacctgt
     9781 caactagtta ataactagtc agaaactagt atagcagtag actcactgta cgcttgaggc
     9841 atcccttcac tcggcagtag acttcatatg gatggatatc aggcacgcca ttgtcgtcct
     9901 gtggactagt cagtaactag gcttaaagct agtcgggtcg gcttactatc ttgaaatccg
     9961 gcagcgtaag ctccccgtcc ttaactgcct cgagatagtg acagtactct ggggactttc
    10021 ggagatcgtt atcgcgaatg ctcggcatac taatcgttga ctagtcttgg actagtcccg
    10081 agcaaaaagg attggaggag gaggaggaag gtgagagtga gacaaagagc gaaataagag
    10141 cttcaaaggc tatctctaag cagtatgaag gttaagtatc tagttcttga ctagatttaa
    10201 aagagatttc gactagttat gtacctggag tttggatata ggaatgtgtt gtggtaacga
    10261 aatgtaaggg ggaggaaaga aaaagtcggt caagaggtaa ctctaagtcg gccattcctt
    10321 tttgggaggc gctaaccata aacggcatgg tcgacttaga gttagctcag ggaatttagg
    10381 gagttatctg cgaccaccga ggaacggcgg aatgccaaag aatcccgatg gagctctagc
    10441 tggcggttga caaccccacc ttttggcgtt tctgcggcgt tgcaggcggg actggatact
    10501 tcgtagaacc agaaaggcaa ggcagaacgc gctcagcaag agtgttggaa gtgatagcat
    10561 gatgtgcctt gttaactagg tcaaaatctg cagtatgctt gatgttatcc aaagtgtgag
    10621 agaggaaggt ccaaacatac acgattggga gagggcctag gtataagagt ttttgagtag
    10681 aacgcatgtg agcccagcca tctcgaggag attaaacacg ggccggcatt tgatggctat
    10741 gttagtaccc caatggaaac ggtgagagtc cagtggtcgc agataactcc ctaaattccc
    10801 tgagctaact ctaagtcgac catgccgttt atggttagcg cctcccaaaa aggaatggcc
    10861 gacttagagt tacctcttga ccgacttttt ctttcctccc ccttacattt cgttaccaca
    10921 acacattcct atatccaaac tccaggtaca taactagtcg aaatctcttt taaatctagt
    10981 caagaactag atacttaacc ttcatactgc ttagagatag cctttgaagc tcttatttcg
    11041 ctctttgtct cactctcacc ttcctcctcc tcctccaatc ctttttgctc gggactagtc
    11101 caagactagt caacgattag tatgccgagc attcgcgata acgatctccg aaagtcccca
    11161 gagtactgtc actatctcga ggcagttaag gacggggagc ttacgctgcc ggatttcaag
    11221 atagtaagcc gacccgacta gctttaagcc tagttactga ctagtccaca ggacgacaat
    11281 ggcgtgcctg atatccatcc atatgaagtc tactgccgag tgaagggatg cctcaagcgt
    11341 acagtgagtc tactgctata ctagtttctg actagttatt aactagttga caggttccct
    11401 ctgccaacag aaacatattg gtcaagcact tgaaggacaa gaactcccac ggcatggagt
    11461 ttacattgca caatggacct cccactatga aggaactgat ggaggccaaa ggcaagtcct
    11521 atctagatta cttggtgact agtccctgac tagtcaagta gcatggtatg aaggcttgtt
    11581 tgaaggcact gttctcccaa ccccgactcc taccaagaag cgcaagcgag ctgcgtaagt
    11641 ttctgtgagt ctaactagtg tattagctaa tatatagcag caccaagtcc aaggaccaca
    11701 atactaaggg tgtcgagaat tcgtgagttt cttctcccat ttcaactagt ccttgactag
    11761 tcactaacta ctttgcagca acgagggtga agctggaaat gatcaggaca atggcgaggg
    11821 cccgtaagta cagccattca atgcagacta gttgctaact aatctgtgac tagttcaagt
    11881 ggtccgtacg ccgtgcatac ccctgtgact ggtaggaatt tgagcaagcc tgtcttgccg
    11941 cgcgatgaga aaggaarggc aagttacatt cagccccgta cctaggatca gtgctaattt
    12001 ataacctcta gccactcttt atgcagatcc gccgtgaggg tagcaaggca gctaaatcag
    12061 ctggtgagaa aggaaccata ccctgcaaga cctgtcgcaa cgcaaagggc aaaggtaagc
    12121 tatccaagct agtttgggac tagattctaa ctagtctcag caccgtgtgg ttcaaagcca
    12181 tattgcgagt tttggcgctt tttctcatcg attgacgagg caaagggagc gagtatgcaa
    12241 cctcgtaagt cagactcaga caaagaccaa ctagttattg accagtcttc tagaaggctc
    12301 tgttgtggat cttgaggccc tggagagttc ctccaacaat ccggagacaa gcaagtcctc
    12361 gtcggactag tcactaacta gactctaact agttgcagac atggataatg caaaagagac
    12421 aagcaatgaa gaaagtggta agacatttct cctttgtggt tctggactag tctttgacta
    12481 gtcacagtct taaacaagga aaatgagcat gaaaatgagg aggaaaaggc tgctgagccc
    12541 gaggaagtgc agggtgatgg cagacatggt aggttaatac cttgttagtt attgctagtc
    12601 actgactagt caataactag tctctgaaca ccttgcaatc actccgtttg cgcagctgaa
    12661 cagtggtgag gataatagta gtaagttatt ctagcttcag agttatagga gactagatac
    12721 taactagtat tagttgcaac taacctggat ctcagagact ttggcctcaa tctagaatct
    12781 atctagttgt caactagact gtggtatcat tgtcttttat tttcctagtc ctggaactag
    12841 cttctaacta gtctccctaa tatgtggctg tcttgttttt tttttttgtt tccctacccg
    12901 gatatctagt ccccttctag gttctgttaa cctctcgggc tctgatttag tttaacgcaa
    12961 acctgagatt agtttctaac tagtctctag gttttctatc cacctttaat tgtaataata
    13021 aatacaagca acgtttatac gtcaaaagca tttataaact tttaccctaa agtagcttgc
    13081 ttgtgtgttt agtttataat tagtctctta ttaatttgat gtaggtaagc ccgccacaaa
    13141 tatatatttt taacaagata ccgtggaaaa acttcgtgct atcacaaaac agtatacaaa
    13201 aaataagct
//
