LOCUS       sequence-483857-        6571 bp    DNA     circular SYN 19-AUG-2025
DEFINITION  Encodes MasR, a receptor on RAAS that antagonizes the AT1R 
            physiological effects..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6571)
  TITLE     Zaid Abassi Lab Plasmids 2025
REFERENCE   2  (bases 1 to 6571)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 19, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MasR-HA
FEATURES             Location/Qualifiers
     source          1..6571
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(56..74)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(57..74)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(94..111)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    100..324
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      370..798
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(457..476)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     667..688
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(807..827)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        812..1141
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      992..1127
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1054..1073
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             1208..2002
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1262..1281)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1872..1891
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    2176..2297
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2213..2232)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2267..2286
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2346..2362)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2346..2362)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2359..2381)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2370..2386
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2394..2400,2401..2418,2419..2424))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2439..2460
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2577..2594)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2748..3336)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2828..2847)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3507..4298,4299..4367))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4130..4149
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4368..4472)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4547..4566)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4738..5117
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5118..5321
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5272..5292
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5366..5385
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5366..5384
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5419..5445
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ctgcagatat ccatcacact ggcggccgct cgagcatgca tctagagggc cctattctat
       61 agtgtcacct aaatgctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc
      121 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
      181 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
      241 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
      301 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
      361 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
      421 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
      481 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
      541 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
      601 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
      661 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
      721 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
      781 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
      841 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
      901 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
      961 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     1021 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     1081 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     1141 aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc
     1201 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     1261 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     1321 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     1381 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     1441 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     1501 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     1561 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     1621 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     1681 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
     1741 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     1801 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     1861 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     1921 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     1981 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
     2041 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     2101 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     2161 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     2221 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     2281 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
     2341 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     2401 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     2461 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     2521 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     2581 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     2641 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     2701 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     2761 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     2821 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     2881 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     2941 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     3001 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     3061 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     3121 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     3181 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     3241 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     3301 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     3361 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     3421 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     3481 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     3541 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     3601 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     3661 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     3721 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     3781 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     3841 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     3901 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     3961 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     4021 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     4081 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     4141 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     4201 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     4261 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     4321 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     4381 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     4441 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     4501 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
     4561 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
     4621 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
     4681 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
     4741 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
     4801 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     4861 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     4921 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     4981 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     5041 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     5101 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     5161 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     5221 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     5281 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac
     5341 ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc caagcttggt
     5401 accgagctcg gatccatgta cccatacgat gttccagatt acgctatgga tgggtcaaac
     5461 gtgacatcat ttgttgttga ggaacccacg aacatctcaa ctggcaggaa cgcctcagtc
     5521 gggaatgcac atcggcaaat ccccatcgtg cactgggtca ttatgagcat ctccccagtg
     5581 gggtttgttg agaatgggat tctcctctgg ttcctgtgct tccggatgag aagaaatccc
     5641 ttcactgtct acatcaccca cctgtctatc gcagacatct cactgctctt ctgtattttc
     5701 atcttgtcta tcgactatgc tttagattat gagctttctt ctggccatta ctacacaatt
     5761 gtcacattat cagtgacttt tctgtttggc tacaacacgg gcctctatct gctgacggcc
     5821 attagtgtgg agaggtgcct gtcagtcctt taccccatct ggtaccgatg ccatcgcccc
     5881 aagtaccagt cggcattggt ctgtgccctt ctgtgggctc tttcttgctt ggtgaccacc
     5941 atggagtatg tcatgtgcat cgacagagaa gaagagagtc actctcggaa tgactgccga
     6001 gcagtcatca tctttatagc catcctgagc ttcctggtct tcacgcccct catgctggtg
     6061 tccagcacca tcttggtcgt gaagatccgg aagaacacgt gggcttccca ttcctccaag
     6121 ctttacatag tcatcatggt caccatcatt atattcctca tcttcgctat gcccatgaga
     6181 ctcctttacc tgctgtacta tgagtattgg tcgacctttg ggaacctaca ccacatttcc
     6241 ctgctcttct ccacaatcaa cagtagcgcc aaccctttca tttacttctt tgtgggaagc
     6301 agtaagaaga agagattcaa ggagtcctta aaagttgttc tgaccagggc tttcaaagat
     6361 gaaatgcaac ctcggcgcca gaaagacaat tgtaatacgg tcacagttga gactgtcgtc
     6421 taagaactgt gagggaagtt gtggataaaa atggtggaac acaggtcatt tttagtttgt
     6481 gcttggaata tgacttaagt atctcctaaa tgtgatacag aagaacatct catcccatat
     6541 gcatgagata ctaattaatg atgaaagaat t
//
