LOCUS       sequence-483902-       12728 bp    DNA     circular SYN 20-AUG-2025
DEFINITION  Expresses NADPH/NADP+ biosensor NAPstar3 in Arabidopsis thaliana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12728)
  AUTHORS   Scherschel M, Niemeier JO, Jacobs LJHC, Hoffmann MDA, Diederich A, 
            Bell C, Hohne P, Raetz S, Kroll JB, Steinbeck J, Lichtenauer S, 
            Multhoff J, Zimmermann J, Sadhanasatish T, Rothemann RA, Grashoff C,
            Messens J, Ampofo E, Laschke MW, Riemer J, Roma LP, Schwarzlander M,
            Morgan B
  TITLE     A family of NADPH/NADP(+) biosensors reveals in vivo dynamics of 
            central redox metabolism across eukaryotes.
  JOURNAL   Nat Commun. 2024 Dec 19;15(1):10704. doi: 
            10.1038/s41467-024-55302-x.
  PUBMED    39702652
REFERENCE   2  (bases 1 to 12728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 20, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-12-19"; volume: "15(1):10704. doi"; pages: " 
            10.1038/s41467-024-55302-x"
COMMENT     Sequence Label: pSS02 NAPstar3
FEATURES             Location/Qualifiers
     source          1..12728
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    40..64
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(82..693,694..714,715..1449,1450..1452,1453..2082,
                     2083..2127)
                     /codon_start=1
                     /product="genetically encoded fluorescent biosensor of the 
                     cytosolic NADH-NAD+ redox state (Hung et al., 2011)"
                     /label=Peredox
                     /note="consists of two pH-insensitive mutant copies of the 
                     Thermus aquaticus redox-sensing repressor Rex (T-Rex) 
                     separated by circularly permuted monomeric T-Sapphire 
                     (cpmTS), a pH-resistant GFP variant"
                     /translation="KVPEAAISRLITYLRILEELEAQGVHRTASEQLGELAQVTAFQVD
                     KDLSYFGSYGTDGVGYTVPVLKRELRHILGLNRKWGLCIVGMGRLGSALADWPGFGESF
                     ELRGFFSRKAEKVGRPVRGGVIEHYDLLPQRVPGRIEIALLTVPREAAQKAADLLVAAG
                     IKGILNFAPVVLEVPKEVAVENVDILAGLTRLSFAILNPTWSAAGGHGFTAHNVYIMAD
                     KQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSIQSKLSKDPNEK
                     RDHMVLLEFVTAAGITHGMDELYKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFS
                     VSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFSYGVMVFARYPDHMKQHDFFKSA
                     MPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNTK
                     VPEAAISRLITYLRILEELEAQGVHRTASEQLGELAQVTAFQVDEDLSYFGSYGTDGVG
                     YTVPVLKRELRHILGLNRKWGLCIVGMGRLGSALADWPGFGESFELRGFFSRKAEKVGR
                     PVRGGVIEHYDLLPQRVPGRIEIALLTVPREAAQKAADLLVAAGIKGILNFAPVVLEVP
                     KEVAVENVDFLAGLTRLSFAILNPKWREEMMGSGTGGNASDGGGSGG"
     CDS             2128..2838
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     2541..2560
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(2840..2864)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      2928..3180
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3197..3213)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3197..3213)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3210..3232)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3221..3237
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3245..3251,3252..3269,3270..3275))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3290..3311
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3502..4178
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     4115..4135
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             4245..5270
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    5311..5485
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    5563..5587
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6012..6806
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      6893..7481
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7382..7401
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7635..7652
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7667..7807
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7733..7752)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7893..7915
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8151..8345
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8411..9484)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9913..10542)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11843..11867
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     12055..12077
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     12069..12086
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     12070..12086
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gggtaccggg ccccccctcg aggcgcgcca agctatcaaa caagtttgta caaaaaagca
       61 ggcttcacca tggttgctag caaagttcct gaagcagcca tttccagact gattacttac
      121 ctgagaattc tggaagagct ggaagcccaa ggtgtacacc gcaccgcctc cgagcaactg
      181 ggcgagctgg cccaggtcac cgccttccag gttgataagg acctgtccta cttcggctcc
      241 tacggcactg acggcgttgg ctacactgta ccggtcctca agagagaact gcgccatatc
      301 ctcggtctga accgcaaatg gggcctgtgc atcgtgggga tgggccgcct gggatccgct
      361 cttgctgatt ggcctggttt tggcgagagc ttcgagctgc gcggtttctt ctctagaaag
      421 gctgagaagg ttggtcggcc ggttcgcggt ggtgttatcg aacactacga tctgctgccc
      481 cagcgcgtac ctggtcgcat cgaaatcgct ctgctgacgg ttccacgcga ggcagcacag
      541 aaggctgccg acctgctggt tgccgctggc atcaagggca tcctgaactt cgctccagtt
      601 gtactggagg ttcccaagga agtggctgtt gagaacgttg acatcctggc cggtctgacg
      661 cgtctgagct ttgccattct gaaccccacg tggagcgcag caggtgggca tggtttcact
      721 gctcacaacg tgtatatcat ggctgacaaa cagaagaacg gtatcaaggc caacttcaag
      781 atccgccaca acatcgaaga cggcggcgtt cagctggctg atcactacca gcagaacacc
      841 ccaatcggcg acggccctgt tctgctgcct gacaaccact acttaagcat ccagtctaaa
      901 ctgtccaaag acccaaacga gaagcgcgac cacatggttc tgctggaatt tgttaccgcc
      961 gctggcatca cccacggcat ggacgaactg tacaagggcg gcaccggggg atctatggtt
     1021 tccaaaggcg aggaactgtt cactggtgtt gttcctatcc tggttgaact ggacggcgac
     1081 gttaacggcc acaaattcag cgttagcggg gaaggcgaag gagacgctac ctacggcaag
     1141 ctgactctga agttcatctg caccactggc aagctgcctg ttccttggcc taccctggtt
     1201 actaccttct cctatggtgt tatggttttc gccagatatc ctgaccacat gaagcagcac
     1261 gacttcttca agagcgccat gcctgagggc tacgttcagg aacgcaccat ctttttcaag
     1321 gacgacggca actataagac acgcgctgag gttaagttcg agggcgacac tctggttaac
     1381 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg ccataagctt
     1441 gaatataaca ccaaggtacc tgaagccgcc atcagccgcc tcatcactta cctccgcatc
     1501 ctggaggagc tcgaagccca aggcgttcac cgcactgcaa gcgagcagct gggcgaactg
     1561 gcacaggtta cagcattcca ggttgacgaa gatctgtcct atttcggttc ctacggcacc
     1621 gacggtgttg gctacacagt ccctgtcctg aagcgcgagc tgcgccacat cctgggcctg
     1681 aaccgcaagt ggggcttatg catcgttggc atgggccgcc tcggtagtgc actggcagac
     1741 tggccgggct ttggtgaatc tttcgaactg cgcggcttct tctcaaggaa ggctgagaag
     1801 gttgggcgcc ctgttcgcgg tggcgttatc gagcattacg acctcctgcc acagcgcgtc
     1861 ccgggtcgca tcgagatcgc cctgctgacc gttcctcgcg aagctgcaca gaaggcagcc
     1921 gacttactag tcgccgctgg tatcaaggga attctgaact tcgcccctgt tgttctggag
     1981 gttcctaagg aagttgctgt tgaaaacgtt gatttcctgg ccggcctgac ccgcctgagc
     2041 ttcgcaatcc tgaatcctaa gtggcgcgaa gaaatgatgg gttccggaac tgggggcaac
     2101 gcctctgacg gtggtgggtc tggtggtatg gtgagcaagg gcgaggagga taacatggcc
     2161 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
     2221 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
     2281 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
     2341 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc
     2401 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
     2461 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
     2521 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
     2581 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
     2641 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
     2701 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag
     2761 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg
     2821 gacgaactgt acaagtgagt cccagctttc ttgtacaaag tggttcgata attcttaatt
     2881 aactagttct agagcggccg ccaccgcggt ggagctcgaa tttccccgat cgttcaaaca
     2941 tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg attatcatat
     3001 aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta
     3061 tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca
     3121 aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg ttactagatc
     3181 gggaattcgt aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     3241 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     3301 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     3361 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt ggctagagca
     3421 gcttgccaac atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt
     3481 ctcagaagac caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct
     3541 cggattccat tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg
     3601 cacctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga
     3661 cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc
     3721 aaccacgtct tcaaagcaag tggattgatg tgaacatggt ggagcacgac actctcgtct
     3781 actccaagaa tatcaaagat acagtctcag aagaccaaag ggctattgag acttttcaac
     3841 aaagggtaat atcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatca
     3901 aaaggacagt agaaaaggaa ggtggcacct acaaatgcca tcattgcgat aaaggaaagg
     3961 ctatcgttca agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga
     4021 gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgata
     4081 tctccactga cgtaagggat gacgcacaat cccactatcc ttcgcaagac ccttcctcta
     4141 tataaggaag ttcatttcat ttggagagga cacgctgaaa tcaccagtct ctctctacaa
     4201 atctatctct ctcgagcttt cgcagatccg gggggcaatg agatatgaaa aagcctgaac
     4261 tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga
     4321 tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat
     4381 atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc
     4441 actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggag tttagcgaga
     4501 gcctgaccta ttgcatctcc cgccgttcac agggtgtcac gttgcaagac ctgcctgaaa
     4561 ccgaactgcc cgctgttcta caaccggtcg cggaggctat ggatgcgatc gctgcggccg
     4621 atcttagcca gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta
     4681 catggcgtga tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga
     4741 tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg
     4801 aggactgccc cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga
     4861 cggacaatgg ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc
     4921 aatacgaggt cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga
     4981 cgcgctactt cgagcggagg catccggagc ttgcaggatc gccacgactc cgggcgtata
     5041 tgctccgcat tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg
     5101 cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc
     5161 gtacacaaat cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg
     5221 ccgatagtgg aaaccgacgc cccagcactc gtccgagggc aaagaaatag agtagatgcc
     5281 gaccgggatc tgtcgatcga caagctcgag tttctccata ataatgtgtg agtagttccc
     5341 agataaggga attagggttc ctatagggtt tcgctcatgt gttgagcata taagaaaccc
     5401 ttagtatgta tttgtatttg taaaatactt ctatcaataa aatttctaat tcctaaaacc
     5461 aaaatccagt actaaaatcc agatcccccg aattaattcg gcgttaattc agtacattaa
     5521 aaacgtccgc aatgtgttat taagttgtct aagcgtcaat ttgtttacac cacaatatat
     5581 cctgccacca gccagccaac agctccccga ccggcagctc ggcacaaaat caccactcga
     5641 tacaggcagc ccatcagtcc gggacggcgt cagcgggaga gccgttgtaa ggcggcagac
     5701 tttgctcatg ttaccgatgc tattcggaag aacggcaact aagctgccgg gtttgaaaca
     5761 cggatgatct cgcggagggt agcatgttga ttgtaacgat gacagagcgt tgctgcctgt
     5821 gatcaccgcg gtttcaaaat cggctccgtc gatactatgt tatacgccaa ctttgaaaac
     5881 aactttgaaa aagctgtttt ctggtattta aggttttaga atgcaaggaa cagtgaattg
     5941 gagttcgtct tgttataatt agcttcttgg ggtatcttta aatactgtag aaaagaggaa
     6001 ggaaataata aatggctaaa atgagaatat caccggaatt gaaaaaactg atcgaaaaat
     6061 accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa ggtatataag ctggtgggag
     6121 aaaatgaaaa cctatattta aaaatgacgg acagccggta taaagggacc acctatgatg
     6181 tggaacggga aaaggacatg atgctatggc tggaaggaaa gctgcctgtt ccaaaggtcc
     6241 tgcactttga acggcatgat ggctggagca atctgctcat gagtgaggcc gatggcgtcc
     6301 tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa gattatcgag ctgtatgcgg
     6361 agtgcatcag gctctttcac tccatcgaca tatcggattg tccctatacg aatagcttag
     6421 acagccgctt agccgaattg gattacttac tgaataacga tctggccgat gtggattgcg
     6481 aaaactggga agaagacact ccatttaaag atccgcgcga gctgtatgat tttttaaaga
     6541 cggaaaagcc cgaagaggaa cttgtctttt cccacggcga cctgggagac agcaacatct
     6601 ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg gagaagcggc agggcggaca
     6661 agtggtatga cattgccttc tgcgtccggt cgatcaggga ggatatcggg gaagaacagt
     6721 atgtcgagct attttttgac ttactgggga tcaagcctga ttgggagaaa ataaaatatt
     6781 atattttact ggatgaattg ttttagtacc tagaatgcat gaccaaaatc ccttaacgtg
     6841 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     6901 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     6961 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     7021 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     7081 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     7141 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     7201 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     7261 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     7321 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     7381 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     7441 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     7501 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     7561 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     7621 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt
     7681 ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct
     7741 gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat
     7801 ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     7861 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     7921 accgtcatca ccgaaacgcg cgaggcaggg tgccttgatg tgggcgccgg cggtcgagtg
     7981 gcgacggcgc ggcttgtccg cgccctggta gattgcctgg ccgtaggcca gccatttttg
     8041 agcggccagc ggccgcgata ggccgacgcg aagcggcggg gcgtagggag cgcagcgacc
     8101 gaagggtagg cgctttttgc agctcttcgg ctgtgcgctg gccagacagt tatgcacagg
     8161 ccaggcgggt tttaagagtt ttaataagtt ttaaagagtt ttaggcggaa aaatcgcctt
     8221 ttttctcttt tatatcagtc acttacatgt gtgaccggtt cccaatgtac ggctttgggt
     8281 tcccaatgta cgggttccgg ttcccaatgt acggctttgg gttcccaatg tacgtgctat
     8341 ccacaggaaa gagacctttt cgaccttttt cccctgctag ggcaatttgc cctagcatct
     8401 gctccgtaca ttaggaaccg gcggatgctt cgccctcgat caggttgcgg tagcgcatga
     8461 ctaggatcgg gccagcctgc cccgcctcct ccttcaaatc gtactccggc aggtcatttg
     8521 acccgatcag cttgcgcacg gtgaaacaga acttcttgaa ctctccggcg ctgccactgc
     8581 gttcgtagat cgtcttgaac aaccatctgg cttctgcctt gcctgcggcg cggcgtgcca
     8641 ggcggtagag aaaacggccg atgccgggat cgatcaaaaa gtaatcgggg tgaaccgtca
     8701 gcacgtccgg gttcttgcct tctgtgatct cgcggtacat ccaatcagct agctcgatct
     8761 cgatgtactc cggccgcccg gtttcgctct ttacgatctt gtagcggcta atcaaggctt
     8821 caccctcgga taccgtcacc aggcggccgt tcttggcctt cttcgtacgc tgcatggcaa
     8881 cgtgcgtggt gtttaaccga atgcaggttt ctaccaggtc gtctttctgc tttccgccat
     8941 cggctcgccg gcagaacttg agtacgtccg caacgtgtgg acggaacacg cggccgggct
     9001 tgtctccctt cccttcccgg tatcggttca tggattcggt tagatgggaa accgccatca
     9061 gtaccaggtc gtaatcccac acactggcca tgccggccgg ccctgcggaa acctctacgt
     9121 gcccgtctgg aagctcgtag cggatcacct cgccagctcg tcggtcacgc ttcgacagac
     9181 ggaaaacggc cacgtccatg atgctgcgac tatcgcgggt gcccacgtca tagagcatcg
     9241 gaacgaaaaa atctggttgc tcgtcgccct tgggcggctt cctaatcgac ggcgcaccgg
     9301 ctgccggcgg ttgccgggat tctttgcgga ttcgatcagc ggccgcttgc cacgattcac
     9361 cggggcgtgc ttctgcctcg atgcgttgcc gctgggcggc ctgcgcggcc ttcaacttct
     9421 ccaccaggtc atcacccagc gccgcgccga tttgtaccgg gccggatggt ttgcgaccgc
     9481 tcacgccgat tcctcgggct tgggggttcc agtgccattg cagggccggc aggcaaccca
     9541 gccgcttacg cctggccaac cgcccgttcc tccacacatg gggcattcca cggcgtcggt
     9601 gcctggttgt tcttgatttt ccatgccgcc tcctttagcc gctaaaattc atctactcat
     9661 ttattcattt gctcatttac tctggtagct gcgcgatgta ttcagatagc agctcggtaa
     9721 tggtcttgcc ttggcgtacc gcgtacatct tcagcttggt gtgatcctcc gccggcaact
     9781 gaaagttgac ccgcttcatg gctggcgtgt ctgccaggct ggccaacgtt gcagccttgc
     9841 tgctgcgtgc gctcggacgg ccggcactta gcgtgtttgt gcttttgctc attttctctt
     9901 tacctcatta actcaaatga gttttgattt aatttcagcg gccagcgcct ggacctcgcg
     9961 ggcagcgtcg ccctcgggtt ctgattcaag aacggttgtg ccggcggcgg cagtgcctgg
    10021 gtagctcacg cgctgcgtga tacgggactc aagaatgggc agctcgtacc cggccagcgc
    10081 ctcggcaacc tcaccgccga tgcgcgtgcc tttgatcgcc cgcgacacga caaaggccgc
    10141 ttgtagcctt ccatccgtga cctcaatgcg ctgcttaacc agctccacca ggtcggcggt
    10201 ggcccatatg tcgtaagggc ttggctgcac cggaatcagc acgaagtcgg ctgccttgat
    10261 cgcggacaca gccaagtccg ccgcctgggg cgctccgtcg atcactacga agtcgcgccg
    10321 gccgatggcc ttcacgtcgc ggtcaatcgt cgggcggtcg atgccgacaa cggttagcgg
    10381 ttgatcttcc cgcacggccg cccaatcgcg ggcactgccc tggggatcgg aatcgactaa
    10441 cagaacatcg gccccggcga gttgcagggc gcgggctaga tgggttgcga tggtcgtctt
    10501 gcctgacccg cctttctggt taagtacagc gataaccttc atgcgttccc cttgcgtatt
    10561 tgtttattta ctcatcgcat catatacgca gcgaccgcat gacgcaagct gttttactca
    10621 aatacacatc acctttttag acggcggcgc tcggtttctt cagcggccaa gctggccggc
    10681 caggccgcca gcttggcatc agacaaaccg gccaggattt catgcagccg cacggttgag
    10741 acgtgcgcgg gcggctcgaa cacgtacccg gccgcgatca tctccgcctc gatctcttcg
    10801 gtaatgaaaa acggttcgtc ctggccgtcc tggtgcggtt tcatgcttgt tcctcttggc
    10861 gttcattctc ggcggccgcc agggcgtcgg cctcggtcaa tgcgtcctca cggaaggcac
    10921 cgcgccgcct ggcctcggtg ggcgtcactt cctcgctgcg ctcaagtgcg cggtacaggg
    10981 tcgagcgatg cacgccaagc agtgcagccg cctctttcac ggtgcggcct tcctggtcga
    11041 tcagctcgcg ggcgtgcgcg atctgtgccg gggtgagggt agggcggggg ccaaacttca
    11101 cgcctcgggc cttggcggcc tcgcgcccgc tccgggtgcg gtcgatgatt agggaacgct
    11161 cgaactcggc aatgccggcg aacacggtca acaccatgcg gccggccggc gtggtggtgt
    11221 cggcccacgg ctctgccagg ctacgcaggc ccgcgccggc ctcctggatg cgctcggcaa
    11281 tgtccagtag gtcgcgggtg ctgcgggcca ggcggtctag cctggtcact gtcacaacgt
    11341 cgccagggcg taggtggtca agcatcctgg ccagctccgg gcggtcgcgc ctggtgccgg
    11401 tgatcttctc ggaaaacagc ttggtgcagc cggccgcgtg cagttcggcc cgttggttgg
    11461 tcaagtcctg gtcgtcggtg ctgacgcggg catagcccag caggccagcg gcggcgctct
    11521 tgttcatggc gtaatgtctc cggttctagt cgcaagtatt ctactttatg cgactaaaac
    11581 acgcgacaag aaaacgccag gaaaagggca gggcggcagc ctgtcgcgta acttaggact
    11641 tgtgcgacat gtcgttttca gaagacggct gcactgaacg tcagaagccg actgcactat
    11701 agcagcggag gggttggatc aaagtacttt gatcccgagg ggaaccctgt ggttggcatg
    11761 cacatacaaa tggacgaacg gataaacctt ttcacgccct tttaaatatc cgattattct
    11821 aataaacgct cttttctctt aggtttaccc gccaatatat cctgtcaaac actgatagtt
    11881 taaactgaag gcgggaaacg acaatctgat ccaagctcaa gctgctctag cattcgccat
    11941 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
    12001 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
    12061 cacgacgttg taaaacgacg gccagtgcca agcttcgacg agtcagtaat aaacggcgtc
    12121 aaagtggttg cagccggcac acacgagtcg tgtttatcaa ctcaaagcac aaatactttt
    12181 cctcaaccta aaaataaggc aattagccaa aaacaacttt gcgtgtaaac aacgctcaat
    12241 acacgtgtca ttttattatt agctattgct tcaccgcctt agctttctcg tgacctagtc
    12301 gtcctcgtct tttcttcttc ttcttctata aaacaatacc caaagagctc ttcttcttca
    12361 caattcagat ttcaatttct caaaatctta aaaactttct ctcaattctc tctaccgtga
    12421 tcaaggtaaa tttctgtgtt ccttattctc tcaaaatctt cgattttgtt ttcgttcgat
    12481 cccaatttcg tatatgttct ttggtttaga ttctgttaat cttagatcga agacgatttt
    12541 ctgggtttga tcgttagata tcatcttaat tctcgattag ggtttcatag atatcatccg
    12601 atttgttcaa ataatttgag ttttgtcgaa taattactct tcgatttgtg atttctatct
    12661 agatctggtg ttagtttcta gtttgtgcga tcgaatttgt agattaatct gagtttttct
    12721 gattaaca
//
