LOCUS       sequence-483910-        8607 bp    DNA     circular SYN 20-AUG-2025
DEFINITION  Express doxycycline inducible CRNDE UCE intron with deletion of the 
            eIF6 binding site F3-2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8607)
  AUTHORS   Lee JS, Dan T, Zhang H, Cheng Y, Rehfeld F, Brugarolas J, Mendell JT
  TITLE     An ultraconserved snoRNA-like element in long noncoding RNA CRNDE 
            promotes ribosome biogenesis and cell proliferation.
  JOURNAL   Mol Cell. 2025 Apr 17;85(8):1543-1560.e10. doi: 
            10.1016/j.molcel.2025.03.006. Epub 2025 Apr 3.
  PUBMED    40185099
REFERENCE   2  (bases 1 to 8607)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 20, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2025.03.006"; journalName: "Mol Cell"; date: 
            "2025-04-17- 17"; volume: "85"; issue: "8"; pages: "1543-1560.e10"
COMMENT     Sequence Label: XLone-UCE intron (DF3-2)
FEATURES             Location/Qualifiers
     source          1..8607
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        9..376
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    17..35
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    53..71
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    89..107
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    125..143
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    161..179
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    197..215
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    233..251
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             442..450
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    3569..3703
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   3979..4013
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(4024..4041)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4024..4040)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4033..4055)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4249..4268)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4368..4390
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4428..4446)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4514..4618
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4619..4687,4688..5479)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4837..4856)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5650..6238
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6139..6158
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6392..6409
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6526..6547
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6562..6567,6568..6585,6586..6592)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6600..6616
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6605..6627
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6624..6640
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6624..6640
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   6666..6728
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    6904..6985
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(7102..7500)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             complement(7501..7557)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(7567..8310)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(8311..8334)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      8334..8343
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(8363..8574)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 atcacctcga gtttactccc tatcagtgat agagaacgta tgaagagttt actccctatc
       61 agtgatagag aacgtatgca gactttactc cctatcagtg atagagaacg tataaggagt
      121 ttactcccta tcagtgatag agaacgtatg accagtttac tccctatcag tgatagagaa
      181 cgtatctaca gtttactccc tatcagtgat agagaacgta tatccagttt actccctatc
      241 agtgatagag aacgtataag ctttgcttat gtaaaccagg gcgcctataa aagagtgctg
      301 attttttgag taaacttcaa ttccacaaca cttttgtctt ataccaactt tccgtaccac
      361 ttcctaccct cgtaaaggta ccagtctgca attcataatg gagctactgt actggctatt
      421 ggaaggagga gattctgaag ataaggaggt aatattatct cttttaaaag aatactttcc
      481 tctgtaatcc tgaatcttta ttacatgtaa gaactttgtg cagtagacag caatttcttt
      541 gaatttggta tatggaaaca attttatttt cctctgctaa gtttttgagc ctgcctcttc
      601 tagtgccatg gactgcattg gtagagctga gaaatatcat ttagccatac tcagcaccct
      661 taaaatagct tctttctgag aattagatct gtgaaggtgt cctgcacagt tcttgtagat
      721 gtcattttag tttgtggttg acgtgcatgc atttagcatg ttgcttaacc gtcctcattc
      781 gcctcccagt tctttgttgc cttcatttgg ggggatgtgt tttctgctgg atgatttact
      841 gctagacatg accaaactct gagtataaga cttggtgttt ggcagctggt tccagtgctc
      901 tgctgagaag tagttgggcc cagcctgggg cattgtagtg tcctggaggc cgggagctcc
      961 tgcacagggg ttcttttcct ggttcagagc cttaggtgcc tttccacttt tcacgtaatc
     1021 ttttcctagg ttggcttgct gcttactttg cagctgttgc agggattcac atggaatgga
     1081 gggctccctt ttattgtgga ttatttcttt gataatttac ccatgtgctt tttcattttc
     1141 aaaaacccag tggtgtttaa gaatgaaaga ttcttgaaga acaaaaattg ggttaaattt
     1201 gtatctatca gaaaagattc tatcctctga tgctatgtag gcacattgaa tatgcaggct
     1261 aaattaaaaa cagagtaaaa cctttttata atatgccaat tactatatct aagaatgttt
     1321 atacaggctg aaattttgta atggtattta tatctgtttt cctttttaag gaaaataata
     1381 tacttttcta ggcatcaaaa atatctgccc ctttcactaa tgctgatgta ccaccttccc
     1441 ccaaccccca acgctaaatt tgactggctt aaaaacatct gccccctgaa ctatatctgg
     1501 ggaggaatat taataaaaca atgaaggact tcatggtgtc tagttatata attaggaaat
     1561 tggttagaaa cgtggctaaa accagtttct ttcattaaaa gaactagtgt acatttaaaa
     1621 acaaaaacct tcaggaaaaa gactctttga tctttaagtc aaatagtatt tttggttaac
     1681 acagcaggaa ggggaaatat accaatttca gattctttta tttatgccta agtagaggtt
     1741 gtaagcagct aaggaagtga ttaaaatgta gtctagtaaa aaatggtgct gattacttgg
     1801 aaagcagttt acatttgcaa aaaaattcag tattatgacc ttcaccaact tttacacatc
     1861 atatacttgg gttattactt atggtatagc ttgtgtatgg aatgcaaggg tattttcaaa
     1921 ttgactaggt cttgctggtc atcttaaagg caattgagac ctttaaactt actttaaaag
     1981 tatgatcttg atgtatatga tactgttttg tctttgctat attaacagaa ttagaggggt
     2041 gttctgcaat tcaaatacct tatatattcc aaattttatt ctctataatg gacttttaaa
     2101 ataaaaggta tatgtgcttc aagagggcaa aatttgaatc atgagctaat ttgctaagca
     2161 tcagattata gaaaagcatc cttgattaat ttggaactgt gaaagggggc gggtaaaact
     2221 gttttctgca gaaatttact agtgcagcaa ccatttaaat taaatgtttg ttaacataat
     2281 agtgatggca ttttctcctc cccctccttg tggttttgtc caactagatg ttacagtggc
     2341 agttgcactg actgttaagt gtttaaatga tgacaccatt atgtgaagtg attttgaaat
     2401 gagagattcc agccaagaat tacatctgct cccatctcct tcaaatcata ctctctggca
     2461 gtacagatta tgattgattt gtttgtgaca gattgcagga aacagtcatt gatttttcaa
     2521 tattttacct taaaattatt tacagttgta accatgggga ggtattttca tgggctgtca
     2581 gcccctgaaa gactaggata atattccctg ctctctgaca agacaaatta cctgtaatga
     2641 gtgcagtagc tgaagggtat acttttattt taaaatatgt caataacccc agtgactaaa
     2701 cgaatattga tttagcataa tgaagcctga gtaacgtgaa aatgagcttt ttcaaggggc
     2761 atggtaaagt ctttcttttt agctggttgt aagaagcttt tgattctttt cagccagctg
     2821 gtaggaatat agaattttat aagcaaacca tcaggaatga tagtgttgtt tctgataagc
     2881 aacatccaaa tattttgacc ctgcttttag tggttttttt caaatcttat tttgagtctt
     2941 acttttagtc atagaatagc tactgatttg atgcggtctt taactgactt aatattttta
     3001 caatttcaat atattttgca ttggaatctc cagtaatgaa tattaaaata tatgtacaat
     3061 catttgtaga tgatatcaat tatattaaga catttcagat gggctattgt agtatttaat
     3121 gtgccgtatt ttatggtaga ataattctca gtctctggac atcaagattg ctttcagtgg
     3181 gaatgaagat taatttactt cagtcctgat tttttaggca tcaatgcatg ttttcatttt
     3241 tgtcagactt ttaccctctt ttaatgtaat tctcaacttc ttatggattt acttcccaat
     3301 acataaaatc cttcaaaaca agaatgataa taatttttat actttttata aaaataaatt
     3361 tatttttagt ccatcaaggt gtctgaagac tagtagacca cctcccctgc gagctaagct
     3421 ggacagccaa tgacgggtaa gagagtgaca tttttcacta acctaagaca ggagggccgt
     3481 cagagctact gcctaatcca aagacgggta aaagtgataa aaatgtatca ctccaaccta
     3541 agacaggcgc agcttccgag ggatttgaga tccagacatg ataagataca ttgatgagtt
     3601 tggacaaacc aaaactagaa tgcagtgaaa aaaatgcctt atttgtgaaa tttgtgatgc
     3661 tattgcctta tttgtaacca ttataagctg caataaacaa gtttgatatc tataacaaga
     3721 aaatatatat ataataagtt atcacgtaag tagaacatga aataacaata taattatcgt
     3781 atgagttaaa tcttaaaagt cacgtaaaag ataatcatgc gtcattttga ctcacgcggt
     3841 cgttatagtt caaaatcagt gacacttacc gcattgacaa gcacgcctca cgggagctcc
     3901 aagcggcgac tgagatgtcc taaatgcaca gcgacggatt cgcgctattt agaaagagag
     3961 agcaatattt caagaatgca tgcgtcaatt ttacgcagac tatctttcta gggttaagaa
     4021 ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     4081 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     4141 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct
     4201 ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc
     4261 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     4321 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     4381 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
     4441 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     4501 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     4561 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     4621 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     4681 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     4741 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     4801 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     4861 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     4921 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     4981 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     5041 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     5101 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     5161 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     5221 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     5281 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     5341 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     5401 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     5461 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     5521 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     5581 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     5641 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     5701 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     5761 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     5821 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     5881 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     5941 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     6001 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     6061 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     6121 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     6181 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     6241 cgccagcaac gcggcctttt tacggtttct ggccttttgc tggccttttg ctcacatgtt
     6301 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     6361 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     6421 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     6481 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     6541 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     6601 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaaggtcga
     6661 cttaacccta gaaagataat catattgtga cgtacgttaa agataatcat gcgtaaaatt
     6721 gacgcatgtg ttttatcggt ctgtatatcg aggtttattt attaatttga atagatatta
     6781 agttttatta tatttacact tacatactaa taataaattc aacaaacaat ttatttatgt
     6841 ttatttattt attaaaaaaa aacaaaaact caaaatttct tctataaagt aacaaaactt
     6901 ttagcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     6961 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     7021 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc
     7081 tgattatgat cctctggaga tttagccctc ccacacataa ccagagggca gcaattcacg
     7141 aatcccaact gccgtcggct gtccatcact gtccttcact atggctttga tcccaggatg
     7201 cagatcgaga agcacctgtc ggcaccgtcc gcaggggctc aagatgcccc tgttctcatt
     7261 tccgatcgcg acgatacaag tcaggttgcc agctgccgca gcagcagcag tgcccagcac
     7321 cacgagttct gcacaaggtc ccccagtaaa atgatataca ttgacaccag tgaagatgcg
     7381 gccgtcgcta gagagagctg cgctggcgac gctgtagtct tcagagatgg ggatgctgtt
     7441 gattgtagcc gttgctcttt caatgagggt ggattcttct tgagacaaag gcttggccat
     7501 ggggccgggg ttctcctcca cgtcgccggc ctgcttcagc aggctgaagt tggtggcgcc
     7561 gctgcccccg gggagcatgt caaggtcaaa atcgtcaaga gcgtcagcag gcagcatatc
     7621 aaggtcaaag tcgtcaaggg catcggctgg gagcatgtct aagtcaaaat cgtcaagggc
     7681 gtcggtcggc ccgccgcttt cgcactttag ctgtttctcc aggccacata tgattagttc
     7741 caggccgaaa aggaaggcag gttcggctcc ctgccggtcg aacagctcaa ttgcttgttt
     7801 cagaagtggg ggcatagaat cggtggtagg tgtctctctt tcctcttttg ctacttgatg
     7861 ctcctgttcc tccaatacgc agcccagtgt aaagtggccc acggcggaca gagcgtacag
     7921 tgcgttctcc agggagaagc cttgctgaca caggaacgcg agctgatttt ccagggtttc
     7981 gtactgtttc tctgttgggc gggtgccgag atgcacttta gccccgtcgc gatgtgagag
     8041 gagagcacag cggtatgact tggcgttgtt ccgcagaaag tcttgccatg actcgccttc
     8101 cagggggcag gagtgggtat gatgcctgtc cagcatctcg attggcaggg catcgagcag
     8161 ggcccgcttg ttcttcacgt gccagtacag ggtaggctgc tcaactccca gcttttgagc
     8221 gagtttcctt gtcgtcaggc cttcgatacc gactccattg agtaattcca gagcagagtt
     8281 tatgactttg ctcttgtcca gtctagacat cttatcgtca tcgtctttgt aatccatggt
     8341 ggcggatccc gcgtcacgac acctgtgttc tggcggcaaa cccgttgcga aaaagaacgt
     8401 tcacggcgac tactgcactt atatacggtt ctcccccacc ctcgggaaaa aggcggagcc
     8461 agtacacgac atcactttcc cagtttaccc cgcgccacct tctctaggca ccggttcaat
     8521 tgccgacccc tccccccaac ttctcgggga ctgtgggcga tgtgcgctct gcccactgac
     8581 gggcaccgga gccactcgag tggaatt
//
