LOCUS       sequence-483946-        1172 bp    DNA     linear   UNA 25-AUG-2025
DEFINITION  Expression of human PHF13 (150-300)with Flag-tag in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Rossi F, Magalhaes AP, Buschow R, Schubert T, Glaser L, Fontana A, 
            Mai J, Staege H, Grimme A, Will H, Schriener S, Hnisz D, Vingron M, 
            Chiariello AM, Kinkley S
  TITLE     Differential oligomerization regulates PHF13 chromatin affinity and 
            function.
  JOURNAL   Nucleic Acids Res. 2025 Jun 20;53(12):gkaf572. doi: 
            10.1093/nar/gkaf572.
  PUBMED    40598901
REFERENCE   2  (bases 1 to 1172)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 25, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2025-06-20"; volume: "53(12):gkaf572. doi"; 
            pages: " 10.1093/nar/gkaf572"
COMMENT     Sequence Label: pFlag-CMV4-PHF13 (150-300)
FEATURES             Location/Qualifiers
     source          1..1172
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             55..78
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    562..1046
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(763..782)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
ORIGIN
        1 tagcagaagc tcgtttagtg aaccgtcaga attaattcag atcgatctac catggactac
       61 aaagacgatg acgacaagct tgcggccgcg aattcgatga cccccacgag tcccaccttg
      121 caggatatcc cccaggctcc cagcgacccc tgctcgggct gggactccga tactccctcg
      181 agtggatctt gtgccactgt gtcacctgat caggtcaaag aaataaaaac tgaaggcaaa
      241 cggactatcg tccggcaggg aaagcaggtg gtgttccgag atgaggacag cactggcaat
      301 gatgaggaca tcatggtgga ctcagatgac gattcctggg acctcgtgac ctgcttctgc
      361 atgaagccat ttgccggccg ccccatgatc gagtgtaatg agtgccacac ctggattcac
      421 ctgtcctgtg cgaaaatccg gaaatccaat gttccagaag tgtttgtctg ccaaaagtgc
      481 cgggactcca agtttgacat ccgccgttcc aaccgctcgc ggacgggctc ccggaagctg
      541 ttcctggact gaccggatcc cgggtggcat ccctgtgacc cctccccagt gcctctcctg
      601 gccctggaag ttgccactcc agtgcccacc agccttgtcc taataaaatt aagttgcatc
      661 attttgtctg actaggtgtc cttctataat attatggggt ggaggggggt gggtatggaa
      721 gcaaggggca agttgggaag acaacctgta gggcctgcgg ggtctattgg gaaccaagct
      781 ggagtgcagt ggcacaatct tggctcactg caatctccgc ctcctgggtt caagcgattc
      841 tcctgcctca gcctcccgag ttgttgggat tccaggcatg catgaccagg ctcagctaat
      901 ttttgttttt ttggtagaga cggggtttca ccatattggc caggctggtc tccaactcct
      961 aatctcaggt gatctaccca ccttggcctc ccaaattgct ggggattaca gggcgtgaaa
     1021 ccactgcctc cctttccctg gtccttctga ttttaaaaat aacctataac caggcaggga
     1081 gggacgttcc agaacacagc ataagggcta cctgggccat gccccaaccc gggtgggaca
     1141 ttttgaattt gcttggcttg ggcactggtc ct
//
