LOCUS       sequence-483947-        1441 bp    DNA     linear   UNA 25-AUG-2025
DEFINITION  Expression of human PHF13  fused to mEYFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Rossi F, Magalhaes AP, Buschow R, Schubert T, Glaser L, Fontana A, 
            Mai J, Staege H, Grimme A, Will H, Schriener S, Hnisz D, Vingron M, 
            Chiariello AM, Kinkley S
  TITLE     Differential oligomerization regulates PHF13 chromatin affinity and 
            function.
  JOURNAL   Nucleic Acids Res. 2025 Jun 20;53(12):gkaf572. doi: 
            10.1093/nar/gkaf572.
  PUBMED    40598901
REFERENCE   2  (bases 1 to 1441)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 25, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2025-06-20"; volume: "53(12):gkaf572. doi"; 
            pages: " 10.1093/nar/gkaf572"
COMMENT     Sequence Label: pEYFP-C1-PHF13
FEATURES             Location/Qualifiers
     source          1..1441
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      63..72
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(69..71,72..74,75..785)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(114..135)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(375..394)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     722..743
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggcggggggg ctttaagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg
       61 tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
      121 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
      181 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
      241 ggcccaccct cgtgaccacc ttcggctacg gcctgcagtg cttcgcccgc taccccgacc
      301 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
      361 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
      421 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
      481 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
      541 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
      601 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
      661 acaaccacta cctgagctac cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc
      721 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
      781 acaagtccgg actcagatct cgagctcaag cttcgaattc catggactct gactcttgcg
      841 ccgccgcctt ccacccggag gaatactccc ccagttgcaa gaggcgcagg accgtggaag
      901 acttcaacaa attctgcacc tttgtcttgg cctatgctgg ctacatccct tatccgaagg
      961 aggaactccc tttaaggagc agccccagcc ctgctaacag cactgctggt accattgaca
     1021 gcgacggctg ggacgcgggt ttctcagaca tcgcgtcctc agtgcccttg ccagtctctg
     1081 accgctgctt tagccacctg cagcctactc tcttgcagcg agccaagccc agtaacttcc
     1141 tgctggacag aaagaaaacg gacaagctga aaaaaaaaaa aaaaagaaac cgcagggaca
     1201 gggatgcccc tgggaaaaaa gggtacaggg ggggcttgtt gaaactggaa accgctgacc
     1261 cctacggggg aaccccccca agtcccccct tgggggaaaa cccccaggtc ccaaaacccc
     1321 ggtggggggg gggaacccaa aactcccccc gggggatttt ggggctgggg cccccgaagg
     1381 gaaaaaaaaa aaaaaagaaa aaaaagaaaa tatctctccc ggggaaaaag aggggtgttt
     1441 t
//
