LOCUS       sequence-483950-        1239 bp    DNA     linear   UNA 20-AUG-2025
DEFINITION  Expression of human PHF13 N terminal domain fused to mEYFP in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Rossi F, Magalhaes AP, Buschow R, Schubert T, Glaser L, Fontana A, 
            Mai J, Staege H, Grimme A, Will H, Schriener S, Hnisz D, Vingron M, 
            Chiariello AM, Kinkley S
  TITLE     Differential oligomerization regulates PHF13 chromatin affinity and 
            function.
  JOURNAL   Nucleic Acids Res. 2025 Jun 20;53(12):gkaf572. doi: 
            10.1093/nar/gkaf572.
  PUBMED    40598901
REFERENCE   2  (bases 1 to 1239)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 20, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2025-06-20"; volume: "53(12):gkaf572. doi"; 
            pages: " 10.1093/nar/gkaf572"
COMMENT     Sequence Label: pEYFP-C1-PHF13_NTD (21-70)
FEATURES             Location/Qualifiers
     source          1..1239
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    34..99
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    385..506
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(422..441)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     476..495
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(513..968)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(650..671)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     862..881
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        995..1099
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1124..1144)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 cgggtcctct cggcatggga cgagctgtac aagtccggac tcagatctcg agctcaagct
       61 tcgaattctg cagtcgacgg taccgcgggc ccgggatcca tgaggcgcag gaccgtggaa
      121 gacttcaaca aattctgcac ctttgtcttg gcctatgctg gctacatccc ttatccgaag
      181 gaggaactcc ctttaaggag cagccccagc cctgctaaca gcactgctgg taccattgac
      241 agcgacggct ggtgaggatc caccggatct agataactga tcataatcag ccataccaca
      301 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
      361 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
      421 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
      481 ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta agcgttaata ttttgttaaa
      541 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
      601 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
      661 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
      721 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
      781 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
      841 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
      901 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaagg cgccgctaca
      961 gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     1021 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     1081 atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta
     1141 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     1201 tagtcagcaa ccaggtgtgg aaagtcccca ggctcccca
//
