LOCUS       sequence-483952-        1214 bp    DNA     linear   UNA 20-AUG-2025
DEFINITION  Expression of human PHF13 with aa 24-40 deletion fused to mEYFP in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Rossi F, Magalhaes AP, Buschow R, Schubert T, Glaser L, Fontana A, 
            Mai J, Staege H, Grimme A, Will H, Schriener S, Hnisz D, Vingron M, 
            Chiariello AM, Kinkley S
  TITLE     Differential oligomerization regulates PHF13 chromatin affinity and 
            function.
  JOURNAL   Nucleic Acids Res. 2025 Jun 20;53(12):gkaf572. doi: 
            10.1093/nar/gkaf572.
  PUBMED    40598901
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 20, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2025-06-20"; volume: "53(12):gkaf572. doi"; 
            pages: " 10.1093/nar/gkaf572"
COMMENT     Sequence Label: pEYFP-C1-PHF13∆24-40
FEATURES             Location/Qualifiers
     source          1..1214
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      54..63
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(60..62,63..65,66..776)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(105..126)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(366..385)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     713..734
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gctttaagca gagctggttt agtgaaccgt cagatccgct agcgctaccg gtcgccacca
       61 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
      121 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
      181 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
      241 tcgtgaccac cttcggctac ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc
      301 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
      361 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
      421 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
      481 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
      541 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
      601 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
      661 acctgagcta ccagtccaag ctgagcaaag accccaacga gaagcgcgat cacatggtcc
      721 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtccg
      781 gactcagatc tcgagctcaa gcttcgaatt ccatggactc tgactcttgc gccgccgcct
      841 tccacccgga ggaatactcc cccagttgca agaggcgcag gtacatccct tatccgaagg
      901 aggaactccc tttaaggagc agccccagcc ctgctaacag cactgctggt accattgaca
      961 gcgacggctg ggacgcgggt ttctcagaca tcgcgtcctc agtgcccttg ccagtctctg
     1021 accgctgctt tagccacctg cagcctactc tcttgcagcg agccaagccc agtaacttcc
     1081 tgctggacag aaagaaaacg gacaagctga agaagaagag aagaagaagc gcagggacag
     1141 tgatgcgcct gggaaagagg gtacaggggg ggcttgctga actggaaccg ctgaccctac
     1201 gggagacccc acga
//
