LOCUS       sequence-483953-        1175 bp    DNA     linear   UNA 20-AUG-2025
DEFINITION  Expression of human PHF13 with aa 24-40 deletion fused to mEYFP in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Rossi F, Magalhaes AP, Buschow R, Schubert T, Glaser L, Fontana A, 
            Mai J, Staege H, Grimme A, Will H, Schriener S, Hnisz D, Vingron M, 
            Chiariello AM, Kinkley S
  TITLE     Differential oligomerization regulates PHF13 chromatin affinity and 
            function.
  JOURNAL   Nucleic Acids Res. 2025 Jun 20;53(12):gkaf572. doi: 
            10.1093/nar/gkaf572.
  PUBMED    40598901
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 20, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2025-06-20"; volume: "53(12):gkaf572. doi"; 
            pages: " 10.1093/nar/gkaf572"
COMMENT     Sequence Label: pEYFP-C1-PHF13∆24-40
FEATURES             Location/Qualifiers
     source          1..1175
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             15..266
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     203..224
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtctatttca tgccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
       61 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
      121 acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccaag ctgagcaaag
      181 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
      241 ctctcggcat ggacgagctg tacaagtccg gactcagatc tcgagctcaa gcttcgaatt
      301 ccatggactc tgactcttgc gccgccgcct tccacccgga ggaatactcc cccagttgca
      361 agaggcgcag gtacatccct tatccgaagg aggaactccc tttaaggagc agccccagcc
      421 ctgctaacag cactgctggt accattgaca gcgacggctg ggacgcgggt ttctcagaca
      481 tcgcgtcctc agtgcccttg ccagtctctg accgctgctt tagccacctg cagcctactc
      541 tcttgcagcg agccaagccc agtaacttcc tgctggacag aaagaaaacg gacaagctga
      601 agaagaagaa gaagaggaag cgcagggaca gtgatgcgcc tgggaaagag gggtacaggg
      661 ggggcttgct gaagctggaa gccgctgacc cctacgtgga gacccccacg agtcccacct
      721 tgcaggatat cccccaggct cccagcgacc cctgctcggg ctgggactcc gatactccct
      781 cgagtggatc ttgtgccact gtgtcacctg atcaggtcaa agaaataaaa actgaaggca
      841 aacggactat cgtccggcag ggaaagcagg tggtgttccg agatgaggac agcactggca
      901 atgatgagga catcatggtg gactcagatg acgattcctg ggacctcgtg acctgcttct
      961 gcatgaagcc atttgccggc cgccccatga tcgagtgtaa tgagtgccac acctggattc
     1021 acctgtcctg tgcgaaaatc cggaaatcca atgttccaga agtgtttgtc tgccaaaagt
     1081 gccgggactc caagtttgac atccgccgtt ccaaccgctc gcggacgggc tcccggaagc
     1141 tgttcctgga ctgaccggat ccaccggatc tgaaa
//
