LOCUS       sequence-483960-        1330 bp    DNA     linear   UNA 20-AUG-2025
DEFINITION  Expression of human PHF13 with aa 24-40 and 272-280 deletion fused 
            to mEYFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Rossi F, Magalhaes AP, Buschow R, Schubert T, Glaser L, Fontana A, 
            Mai J, Staege H, Grimme A, Will H, Schriener S, Hnisz D, Vingron M, 
            Chiariello AM, Kinkley S
  TITLE     Differential oligomerization regulates PHF13 chromatin affinity and 
            function.
  JOURNAL   Nucleic Acids Res. 2025 Jun 20;53(12):gkaf572. doi: 
            10.1093/nar/gkaf572.
  PUBMED    40598901
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 20, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2025-06-20"; volume: "53(12):gkaf572. doi"; 
            pages: " 10.1093/nar/gkaf572"
COMMENT     Sequence Label: pEYFP-C1-PHF13 ∆24-40∆272-280
FEATURES             Location/Qualifiers
     source          1..1330
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             177..428
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     365..386
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 caggggcgcc ctttttttca aggggagcgg aaattcaaaa cccggccgag ggaaatttga
       61 ggcggacccc ctgggaaccg catcgagctg aagggcattg acttcaagga ggacggcaac
      121 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catgccgaca
      181 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
      241 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
      301 ccgacaacca ctacctgagc taccagtcca agctgagcaa agaccccaac gagaagcgcg
      361 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
      421 tgtacaagtc cggactcaga tctcgagctc aagctttcat ggactctgac tcttgcgccg
      481 ccgccttcca cccggaggaa tactccccca gttgcaagag gcgcaggtac atcccttatc
      541 cgaaggagga actcccttta aggagcagcc ccagccctgc taacagcact gctggtacca
      601 ttgacagcga cggctgggac gcgggtttct cagacatcgc gtcctcagtg cccttgccag
      661 tctctgaccg ctgctttagc cacctgcagc ctactctctt gcagcgagcc aagcccagta
      721 acttcctgct ggacagaaag aaaacggaca agctgaagaa gaagaagaag aggaagcgca
      781 gggacagtga tgcgcctggg aaagaggggt acaggggggg cttgctgaag ctggaagccg
      841 ctgaccccta cgtggagacc cccacgagtc ccaccttgca ggatatcccc caggctccca
      901 gcgacccctg ctcgggctgg gactccgata ctccctcgag tggatcttgt gccactgtgt
      961 cacctgatca ggtcaaagaa ataaaaactg aaggcaaacg gactatcgtc cggcagggaa
     1021 agcaggtggt gttccgagat gaggacagca ctggcaatga tgaggacatc atggtggact
     1081 cagatgacga ttcctgggac ctcgtgacct gcttctgcat gaagccattt gccggccgcc
     1141 ccatgatcga gtgtaatgag tgccacacct ggattcacct gtcctgtgcg aaaatccgga
     1201 aatccaatgt tccagaagtg aagtttgaca tccgccgttc caaccgctcg cggacgggct
     1261 cccggaagct gttcctggac tgaccggatc caccggatct agataactga tcatattcag
     1321 ccaaacctcc
//
