LOCUS       sequence-483961-        1180 bp    DNA     linear   UNA 25-AUG-2025
DEFINITION  Expression of human PHF13 fused to ECFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Rossi F, Magalhaes AP, Buschow R, Schubert T, Glaser L, Fontana A, 
            Mai J, Staege H, Grimme A, Will H, Schriener S, Hnisz D, Vingron M, 
            Chiariello AM, Kinkley S
  TITLE     Differential oligomerization regulates PHF13 chromatin affinity and 
            function.
  JOURNAL   Nucleic Acids Res. 2025 Jun 20;53(12):gkaf572. doi: 
            10.1093/nar/gkaf572.
  PUBMED    40598901
REFERENCE   2  (bases 1 to 1180)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 25, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2025-06-20"; volume: "53(12):gkaf572. doi"; 
            pages: " 10.1093/nar/gkaf572"
COMMENT     Sequence Label: pECFP-C1-PHF13
FEATURES             Location/Qualifiers
     source          1..1180
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      51..60
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(57..59,60..62,63..773)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(102..123)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(363..382)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     710..731
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atagcagaag ctggtttagt gaaccgtcag atccgctagc gctaccggtc gccaccatgg
       61 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
      121 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
      181 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
      241 tgaccaccct gacctggggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
      301 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
      361 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
      421 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
      481 tggagtacaa ctacatcagc cacaacgtct atatcaccgc cgacaagcag aagaacggca
      541 tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
      601 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
      661 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
      721 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtccggac
      781 tcagatctcg agctcaagct tcgaattcca tggactctga ctcttgcgcc gccgccttcc
      841 acccggagga atactccccc agttgcaaga ggcgcaggac cgtggaagac ttcaacaaat
      901 tctgcacctt tgtcttggcc tatgctggct acatccctta tccgaaggag gaactccctt
      961 taaggagcag ccccagccct gctaacagca ctgctggtac cattgacagc gacggctggg
     1021 acgcgggttt ctcagacatc gcgtcctcag tgcccttgcc agtctctgac cgctgcttta
     1081 gccacctgca gcctactctc ttgcagcgag ccaagcccag taacttcctg ctggacagaa
     1141 agaaaaacgg acaagctgaa aaaaaaaaaa aaaaaagaaa
//
