LOCUS       sequence-483965-        1260 bp    DNA     linear   UNA 20-AUG-2025
DEFINITION  Expression of human PHF13 with PEST2 deletion fused to ECFP in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Rossi F, Magalhaes AP, Buschow R, Schubert T, Glaser L, Fontana A, 
            Mai J, Staege H, Grimme A, Will H, Schriener S, Hnisz D, Vingron M, 
            Chiariello AM, Kinkley S
  TITLE     Differential oligomerization regulates PHF13 chromatin affinity and 
            function.
  JOURNAL   Nucleic Acids Res. 2025 Jun 20;53(12):gkaf572. doi: 
            10.1093/nar/gkaf572.
  PUBMED    40598901
REFERENCE   2  (bases 1 to 1260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 20, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2025-06-20"; volume: "53(12):gkaf572. doi"; 
            pages: " 10.1093/nar/gkaf572"
COMMENT     Sequence Label: pECFP-C1-PHF13∆PEST2 (del141-190)
FEATURES             Location/Qualifiers
     source          1..1260
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      54..63
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(60..62,63..65,66..776)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(105..126)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(366..385)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     713..734
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggctatagca gagctggttt agtgaaccgt cagatccgct agcgctaccg gtcgccacca
       61 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
      121 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
      181 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
      241 tcgtgaccac cctgacctgg ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
      301 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
      361 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
      421 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
      481 agctggagta caactacatc agccacaacg tctatatcac cgccgacaag cagaagaacg
      541 gcatcaaggc caacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
      601 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
      661 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
      721 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtccg
      781 gactcagatc catggactct gactcttgcg ccgccgcctt ccacccggag gaatactccc
      841 ccagttgcaa gaggcgcagg accgtggaag acttcaacaa attctgcacc tttgtcttgg
      901 cctatgctgg ctacatccct tatccgaagg aggaactccc tttaaggagc agccccagcc
      961 ctgctaacag cactgctggt accattgaca gcgacggctg ggacgcgggt ttctcagaca
     1021 tcgcgtcctc agtgcccttg ccagtctctg accgctgctt tagccacctg cagcctactc
     1081 tcttgcagcg agccaagccc agtaacttcc tgctggacag aaagaaaacg gacaagctga
     1141 agaagaaaaa aaaaagaaac gcagggacag tgatgcgcct gggaaaaaag ggtacagggg
     1201 gggcttgctg aaagaaataa aactgagggc aacggactat cgtccggcag gaaagcaggg
//
