LOCUS       sequence-483969-        1181 bp    DNA     linear   UNA 25-AUG-2025
DEFINITION  Expression of human PHF13 (150-300) fused to ECFP in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Rossi F, Magalhaes AP, Buschow R, Schubert T, Glaser L, Fontana A, 
            Mai J, Staege H, Grimme A, Will H, Schriener S, Hnisz D, Vingron M, 
            Chiariello AM, Kinkley S
  TITLE     Differential oligomerization regulates PHF13 chromatin affinity and 
            function.
  JOURNAL   Nucleic Acids Res. 2025 Jun 20;53(12):gkaf572. doi: 
            10.1093/nar/gkaf572.
  PUBMED    40598901
REFERENCE   2  (bases 1 to 1181)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 25, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2025-06-20"; volume: "53(12):gkaf572. doi"; 
            pages: " 10.1093/nar/gkaf572"
COMMENT     Sequence Label: pECFP-C1-PHF13 (150-300)
FEATURES             Location/Qualifiers
     source          1..1181
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      52..61
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(58..60,61..63,64..774)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(103..124)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(364..383)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     711..732
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tataagcaga gctggtttag tgaaccgtca gatccgctag cgctaccggt cgccaccatg
       61 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
      121 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
      181 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
      241 gtgaccaccc tgacctgggg cgtgcagtgc ttcagccgct accccgacca catgaagcag
      301 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
      361 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
      421 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
      481 ctggagtaca actacatcag ccacaacgtc tatatcaccg ccgacaagca gaagaacggc
      541 atcaaggcca acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
      601 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
      661 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
      721 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtccgga
      781 ctcagatctc gagctcaagc ttcgaattcg atgaccccca cgagtcccac cttgcaggat
      841 atcccccagg ctcccagcga cccctgctcg ggctgggact ccgatactcc ctcgagtgga
      901 tcttgtgcca ctgtgtcacc tgatcaggtc aaagaaataa aaactgaagg caaacggact
      961 atcgtccggc agggaaagca ggtggtgttc cgagatgagg acagcactgg caatgatgag
     1021 gacatcatgg tggactcaga tgacgattcc tgggacctcg tgacctgctt ctgcatgaag
     1081 ccatttgccg gccgccccat gatcgagtgt aatgagtgcc acacctggat tcacctgtcc
     1141 tgtgcgaaaa tccggaaatc caatgttcca gaagggtttg t
//
