LOCUS       sequence-483971-        1159 bp    DNA     linear   UNA 25-AUG-2025
DEFINITION  Expression of human PHF13 (100-200) fused to ECFP in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Rossi F, Magalhaes AP, Buschow R, Schubert T, Glaser L, Fontana A, 
            Mai J, Staege H, Grimme A, Will H, Schriener S, Hnisz D, Vingron M, 
            Chiariello AM, Kinkley S
  TITLE     Differential oligomerization regulates PHF13 chromatin affinity and 
            function.
  JOURNAL   Nucleic Acids Res. 2025 Jun 20;53(12):gkaf572. doi: 
            10.1093/nar/gkaf572.
  PUBMED    40598901
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 25, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2025-06-20"; volume: "53(12):gkaf572. doi"; 
            pages: " 10.1093/nar/gkaf572"
COMMENT     Sequence Label: pECFP-C1-PHF13 (100-200)
FEATURES             Location/Qualifiers
     source          1..1159
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      53..62
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(59..61,62..64,65..775)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(104..125)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(365..384)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     712..733
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg tcgccaccat
       61 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
      121 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
      181 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
      241 cgtgaccacc ctgacctggg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
      301 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
      361 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
      421 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
      481 gctggagtac aactacatca gccacaacgt ctatatcacc gccgacaagc agaagaacgg
      541 catcaaggcc aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
      601 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
      661 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
      721 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtccgg
      781 actcagatct cgagctcaag cttcgaattc gatggccaag cccagtaact tcctgctgga
      841 cagaaagaaa acggacaagc tgaagaagaa gaagaagagg aagcgcaggg acagtgatgc
      901 gcctgggaaa gaggggtaca gggggggctt gctgaagctg gaagccgctg acccctacgt
      961 ggagaccccc acgagtccca ccttgcagga tatcccccag gctcccagcg acccctgctc
     1021 gggctgggac tccgatactc cctcgagtgg atcttgtgcc actgtgtcac ctgatcaggt
     1081 caaagaaata aaaactgaag gcaaacggac tatctgagga tccaccggat ctagataact
     1141 gatcataatc agccatacc
//
