LOCUS       sequence-483972-        1235 bp    DNA     linear   UNA 25-AUG-2025
DEFINITION  Expression of human PHF13 (100-200) fused to ECFP in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Rossi F, Magalhaes AP, Buschow R, Schubert T, Glaser L, Fontana A, 
            Mai J, Staege H, Grimme A, Will H, Schriener S, Hnisz D, Vingron M, 
            Chiariello AM, Kinkley S
  TITLE     Differential oligomerization regulates PHF13 chromatin affinity and 
            function.
  JOURNAL   Nucleic Acids Res. 2025 Jun 20;53(12):gkaf572. doi: 
            10.1093/nar/gkaf572.
  PUBMED    40598901
REFERENCE   2  (bases 1 to 1235)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 25, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2025-06-20"; volume: "53(12):gkaf572. doi"; 
            pages: " 10.1093/nar/gkaf572"
COMMENT     Sequence Label: pECFP-C1-PHF13 (100-200)
FEATURES             Location/Qualifiers
     source          1..1235
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        61..112
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     63..83
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      151..160
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(157..159,160..162,163..873)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(202..223)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(463..482)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     810..831
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gagtttgttt ggcaccaaat caacgggact ttccaaaatt tgtaacaatt ccgccccatt
       61 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt
      121 gaaccgtcag atccgctagc gctaccggtc gccaccatgg tgagcaaggg cgaggagctg
      181 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
      241 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
      301 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctggggc
      361 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
      421 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
      481 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
      541 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacatcagc
      601 cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc
      661 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
      721 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
      781 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
      841 gggatcactc tcggcatgga cgagctgtac aagtccggac tcagatctcg agctcaagct
      901 tcgaattcga tggccaagcc cagtaacttc ctgctggaca gaaagaaaac ggacaagctg
      961 aagaagaaga agaagaggaa gcgcagggac agtgatgcgc ctgggaaaga ggggtacagg
     1021 gggggcttgc tgaagctgga agccgctgac ccctacgtgg agacccccac gagtcccacc
     1081 ttgcaggata tcccccaggc tcccagcgac ccctgctcgg gctgggactc cgatactccc
     1141 tcgagtggat cttgtgccac tgtgtcacct gatcaggtca aagaaataaa aactgaaggc
     1201 aaacggacta tctgaggatc caccggatct gatac
//
