LOCUS       sequence-483976-        1200 bp    DNA     linear   UNA 25-AUG-2025
DEFINITION  Expression of human PHF13 N terminal domain fused to ECFP in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Rossi F, Magalhaes AP, Buschow R, Schubert T, Glaser L, Fontana A, 
            Mai J, Staege H, Grimme A, Will H, Schriener S, Hnisz D, Vingron M, 
            Chiariello AM, Kinkley S
  TITLE     Differential oligomerization regulates PHF13 chromatin affinity and 
            function.
  JOURNAL   Nucleic Acids Res. 2025 Jun 20;53(12):gkaf572. doi: 
            10.1093/nar/gkaf572.
  PUBMED    40598901
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 25, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2025-06-20"; volume: "53(12):gkaf572. doi"; 
            pages: " 10.1093/nar/gkaf572"
COMMENT     Sequence Label: pECFP-C1-PHF13_NTD (21-70)
FEATURES             Location/Qualifiers
     source          1..1200
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        102..201
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     152..172
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      240..249
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(246..248,249..251,252..962)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(291..312)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(552..571)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     899..920
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    963..1028
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 atgcggtttt ggcagtacat caatggggcg ggatagcggt ttgactcacg gggatttcca
       61 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
      121 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
      181 gaggtctata taagcagagc tggtttagtg aaccgtcaga tccgctagcg ctaccggtcg
      241 ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
      301 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
      361 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
      421 ccaccctcgt gaccaccctg acctggggcg tgcagtgctt cagccgctac cccgaccaca
      481 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
      541 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
      601 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
      661 ggcacaagct ggagtacaac tacatcagcc acaacgtcta tatcaccgcc gacaagcaga
      721 agaacggcat caaggccaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
      781 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
      841 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
      901 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
      961 agtccggact cagatctcga gctcaagctt cgaattctgc agtcgacggt accgcgggcc
     1021 cgggatccat gaggcgcagg accgtggaag acttcaacaa attctgcacc tttgtcttgg
     1081 cctatgctgg ctacatccct tatccgaagg aggaactccc tttaaggagc agccccagcc
     1141 ctgctaacag cactgctggt accattgaca gcgacggctg gtgaggatcc accggatcag
//
