LOCUS       sequence-483981-        1193 bp    DNA     linear   UNA 25-AUG-2025
DEFINITION  Expression of human PHF13 with N terminal tet operator in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Rossi F, Magalhaes AP, Buschow R, Schubert T, Glaser L, Fontana A, 
            Mai J, Staege H, Grimme A, Will H, Schriener S, Hnisz D, Vingron M, 
            Chiariello AM, Kinkley S
  TITLE     Differential oligomerization regulates PHF13 chromatin affinity and 
            function.
  JOURNAL   Nucleic Acids Res. 2025 Jun 20;53(12):gkaf572. doi: 
            10.1093/nar/gkaf572.
  PUBMED    40598901
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 25, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2025-06-20"; volume: "53(12):gkaf572. doi"; 
            pages: " 10.1093/nar/gkaf572"
COMMENT     Sequence Label: pCDNA-4TO-PHF13
FEATURES             Location/Qualifiers
     source          1..1193
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     302..324
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             351..368
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 atacgggttt cgagaattgc atatagtaaa gactttgaaa ctttcaaagt tgatttctta
       61 gcaagctacc tgaaatgctg aaaatgttcg aagatcgttt atgtcataaa acatatttaa
      121 atggtgatca tgtaacccat cctgacttca tgttgtatga cgctcttgat gttgttttat
      181 acatggaccc aatgtgcctg gatgcgttcc caaaattagt ttgttttaaa aaacgtattg
      241 aagctatccc acaaattgat aagtacttga aatccagcaa gtatatagca tggcctttgc
      301 agggctggca agccacgttt ggtggtggcg accatcctcc aaaatcggat ctggttccgc
      361 gtggatccat ggactctgac tcttgcgccg ccgccttcca cccggaggaa tactccccca
      421 gttgcgagag gcgcaggacc gtggaagact tcaacaaatt ctgcaccttt gtcttggcct
      481 atgctggcta catcccttat ccgaaggagg aactcccttt aaggagcagc cccagccctg
      541 ctaacagcac tgctggtacc attgacagcg acggctggga cgcgggtttc tcagacatcg
      601 cgtcctcagt gcccttgcca gtctctgacc gctgctttag ccacctgcag cctactctct
      661 tgcagcgagc caagcccagt aacttcctgc tggacagaaa gaaaacggac aagctgaaga
      721 agaagaagaa gaggaagcgc agggacagtg atgcgcctgg gaaagagggg tacagggggg
      781 gcttgctgaa gctggaagcc gctgacccct acgtggagac ccccacgagt cccaccttgc
      841 aggatatccc ccaggctccc agcgacccct gctcgggctg ggactccgat actccctcga
      901 gtggatcttg tgccactgtg tcacctgatc aggtcaaaga aataaaaact gaaggcaaac
      961 ggactatcgt ccggcaggga aagcaggtgg tgttccgaga tgaggacagc actggcaatg
     1021 atgaggacat catggtggac tcagatgacg attcctggga caagtttgac atccgccgtt
     1081 ccaaccgctc gcggacgggc tcccggaagc tgttcctgga ctgaccgaat tcccgggtcg
     1141 actcgagcgg ccgcatcgtg actgactgac gatctgcctc gcgcgttcgg aag
//
