LOCUS       sequence-483982-        1187 bp    DNA     linear   UNA 20-AUG-2025
DEFINITION  Expression of human PHF13 with N terminal domain deletion and N 
            terminal tet operator in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Rossi F, Magalhaes AP, Buschow R, Schubert T, Glaser L, Fontana A, 
            Mai J, Staege H, Grimme A, Will H, Schriener S, Hnisz D, Vingron M, 
            Chiariello AM, Kinkley S
  TITLE     Differential oligomerization regulates PHF13 chromatin affinity and 
            function.
  JOURNAL   Nucleic Acids Res. 2025 Jun 20;53(12):gkaf572. doi: 
            10.1093/nar/gkaf572.
  PUBMED    40598901
REFERENCE   2  (bases 1 to 1187)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 20, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2025-06-20"; volume: "53(12):gkaf572. doi"; 
            pages: " 10.1093/nar/gkaf572"
COMMENT     Sequence Label: pCDNA-4TO-PHF13∆NTD (del21-70)
FEATURES             Location/Qualifiers
     source          1..1187
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    22..40
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    43..61
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     71..95
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(1052..1069)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1058..1168
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ttggggcaat aagcagagct ctccctatca gtgatagaga tctccctatc agtgatagag
       61 atcgtcgacg agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt
      121 ttgacctcca tagaagacac cgggaccgat ccagcctccg gactctagcg tttaaactta
      181 agcttggtac cgagctcgga tccatggact ctgactcttg cgccgccgcc ttccacccgg
      241 aggaatactc ccccagttgc gaggacgcgg gtttctcaga catcgcgtcc tcagtgccct
      301 tgccagtctc tgaccgctgc tttagccacc tgcagcctac tctcttgcag cgagccaagc
      361 ccagtaactt cctgctggac agaaagaaaa cggacaagct gaagaagaag aagaagagga
      421 agcgcaggga cagtgatgcg cctgggaaag aggggtacag ggggggcttg ctgaagctgg
      481 aagccgctga cccctacgtg gagaccccca cgagtcccac cttgcaggat atcccccagg
      541 ctcccagcga cccctgctcg ggctgggact ccgatactcc ctcgagtgga tcttgtgcca
      601 ctgtgtcacc tgatcaggtc aaagaaataa aaactgaagg caaacggact atcgtccggc
      661 agggaaagca ggtggtgttc cgagatgagg acagcactgg caatgatgag gacatcatgg
      721 tggactcaga tgacgattcc tgggacctcg tgacctgctt ctgcatgaag ccatttgccg
      781 gccgccccat gatcgagtgt aatgagtgcc acacctggat tcacctgtcc tgtgcgaaaa
      841 tccggaaatc caatgttcca gaagtgtttg tctgccaaaa gtgccgggac tccaagtttg
      901 acatccgccg ttccaaccgc tcgcggacgg gctcccggaa gctgttcctg gactgaccgg
      961 atccactagt ccagtgtggt ggaattctgc agatatccag cacagtggcg gccgctcgag
     1021 tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
     1081 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     1141 ctttcctata aaatgaggaa attgcatcgc attgtctgaa taggtgt
//
