LOCUS       sequence-483983-        1197 bp    DNA     linear   UNA 20-AUG-2025
DEFINITION  Expression of human PHF13 with N terminal domain deletion and N 
            terminal tet operator in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Rossi F, Magalhaes AP, Buschow R, Schubert T, Glaser L, Fontana A, 
            Mai J, Staege H, Grimme A, Will H, Schriener S, Hnisz D, Vingron M, 
            Chiariello AM, Kinkley S
  TITLE     Differential oligomerization regulates PHF13 chromatin affinity and 
            function.
  JOURNAL   Nucleic Acids Res. 2025 Jun 20;53(12):gkaf572. doi: 
            10.1093/nar/gkaf572.
  PUBMED    40598901
REFERENCE   2  (bases 1 to 1197)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 20, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2025-06-20"; volume: "53(12):gkaf572. doi"; 
            pages: " 10.1093/nar/gkaf572"
COMMENT     Sequence Label: pCDNA-4TO-PHF13∆NTD (del21-70)
FEATURES             Location/Qualifiers
     source          1..1197
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        73..172
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     123..143
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    174..192
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    195..213
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     223..247
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
       61 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
      121 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctccctat
      181 cagtgataga gatctcccta tcagtgatag agatcgtcga cgagctcgtt tagtgaaccg
      241 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg
      301 atccagcctc cggactctag cgtttaaact taagcttggt accgagctcg gatccatgga
      361 ctctgactct tgcgccgccg ccttccaccc ggaggaatac tcccccagtt gcgaggacgc
      421 gggtttctca gacatcgcgt cctcagtgcc cttgccagtc tctgaccgct gctttagcca
      481 cctgcagcct actctcttgc agcgagccaa gcccagtaac ttcctgctgg acagaaagaa
      541 aacggacaag ctgaagaaga agaagaagag gaagcgcagg gacagtgatg cgcctgggaa
      601 agaggggtac agggggggct tgctgaagct ggaagccgct gacccctacg tggagacccc
      661 cacgagtccc accttgcagg atatccccca ggctcccagc gacccctgct cgggctggga
      721 ctccgatact ccctcgagtg gatcttgtgc cactgtgtca cctgatcagg tcaaagaaat
      781 aaaaactgaa ggcaaacgga ctatcgtccg gcagggaaag caggtggtgt tccgagatga
      841 ggacagcact ggcaatgatg aggacatcat ggtggactca gatgacgatt cctgggacct
      901 cgtgacctgc ttctgcatga agccatttgc cggccgcccc atgatcgagt gtaatgagtg
      961 ccacacctgg attcacctgt cctgtgcgaa aatccggaaa tccaatgttc cagaagtgtt
     1021 tgtctgccaa aagtgccggg actccaagtt tgacatccgc cgttccaacc gctcgcggac
     1081 gggctcccgg aagctgttcc tggactgacc ggatccacta gtccagtgtg gtggaattct
     1141 gcagatatcc agcacagtgg cggccgctcg agtctagagg gcccgttaac ccctctc
//
