LOCUS       sequence-483986-        1193 bp    DNA     linear   UNA 20-AUG-2025
DEFINITION  Expression of human PHF13 with PEST2 deletion and N terminal tet 
            operator in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Rossi F, Magalhaes AP, Buschow R, Schubert T, Glaser L, Fontana A, 
            Mai J, Staege H, Grimme A, Will H, Schriener S, Hnisz D, Vingron M, 
            Chiariello AM, Kinkley S
  TITLE     Differential oligomerization regulates PHF13 chromatin affinity and 
            function.
  JOURNAL   Nucleic Acids Res. 2025 Jun 20;53(12):gkaf572. doi: 
            10.1093/nar/gkaf572.
  PUBMED    40598901
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 20, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2025-06-20"; volume: "53(12):gkaf572. doi"; 
            pages: " 10.1093/nar/gkaf572"
COMMENT     Sequence Label: pCDNA-4TO-PHF13∆PEST2 (del141-190)
FEATURES             Location/Qualifiers
     source          1..1193
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    9..27
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     37..61
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(999..1016)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1005..1116
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ggtaagtctc cctatcagtg atagagatcg tcgacgagct cgtttagtga accgtcagat
       61 cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg accgatccag
      121 cctccggact ctagcgttta aacttaagct tggtaccgag ctcggatcca tggactctga
      181 ctcttgcgcc gccgccttcc acccggagga atactccccc agttgcaaga ggcgcaggac
      241 cgtggaagac ttcaacaaat tctgcacctt tgtcttggcc tatgctggct acatccctta
      301 tccgaaggag gaactccctt taaggagcag ccccagccct gctaacagca ctgctggtac
      361 cattgacagc gacggctggg acgcgggttt ctcagacatc gcgtcctcag tgcccttgcc
      421 agtctctgac cgctgcttta gccacctgca gcctactctc ttgcagcgag ccaagcccag
      481 taacttcctg ctggacagaa agaaaacgga caagctgaag aagaagaaga agaggaagcg
      541 cagggacagt gatgcgcctg ggaaagaggg gtacaggggg ggcttgctga aagaaataaa
      601 aactgaaggc aaacggacta tcgtccggca gggaaagcag gtggtgttcc gagatgagga
      661 cagcactggc aatgatgagg acatcatggt ggactcagat gacgattcct gggacctcgt
      721 gacctgcttc tgcatgaagc catttgccgg ccgccccatg atcgagtgta atgagtgcca
      781 cacctggatt cacctgtcct gtgcgaaaat ccggaaatcc aatgttccag aagtgtttgt
      841 ctgccaaaag tgccgggact ccaagtttga catccgccgt tccaaccgct cgcggacggg
      901 ctcccggaag ctgttcctgg actgaccgga attctgcaga tatccagcac agtggcggcc
      961 gctcgagtct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
     1021 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     1081 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcatct
     1141 attctggggg gtgggggggg gcaagacagc aggggggagg attgggaaga cta
//
