LOCUS       sequence-483988-        1166 bp    DNA     linear   UNA 25-AUG-2025
DEFINITION  Expression of human PHF13 (M246A) with N terminal tet operator in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Rossi F, Magalhaes AP, Buschow R, Schubert T, Glaser L, Fontana A, 
            Mai J, Staege H, Grimme A, Will H, Schriener S, Hnisz D, Vingron M, 
            Chiariello AM, Kinkley S
  TITLE     Differential oligomerization regulates PHF13 chromatin affinity and 
            function.
  JOURNAL   Nucleic Acids Res. 2025 Jun 20;53(12):gkaf572. doi: 
            10.1093/nar/gkaf572.
  PUBMED    40598901
REFERENCE   2  (bases 1 to 1166)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 25, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2025-06-20"; volume: "53(12):gkaf572. doi"; 
            pages: " 10.1093/nar/gkaf572"
COMMENT     Sequence Label: pCDNA-4TO-PHF13M246A
FEATURES             Location/Qualifiers
     source          1..1166
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    15..33
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    36..54
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     64..88
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 tttaagcaga gctctcccta tcagtgatag agatctccct atcagtgata gagatcgtcg
       61 acgagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
      121 ccatagaaga caccgggacc gatccagcct ccggactcta gcgtttaaac ttaagcttgg
      181 taccgagctc ggatccatgg actctgactc ttgcgccgcc gccttccacc cggaggaata
      241 ctcccccagt tgcaagaggc gcaggaccgt ggaagacttc aacaaattct gcacctttgt
      301 cttggcctat gctggctaca tcccttatcc gaaggaggaa ctccctttaa ggagcagccc
      361 cagccctgct aacagcactg ctggtaccat tgacagcgac ggctgggacg cgggtttctc
      421 agacatcgcg tcctcagtgc ccttgccagt ctctgaccgc tgctttagcc acctgcagcc
      481 tactctcttg cagcgagcca agcccagtaa cttcctgctg gacagaaaga aaacggacaa
      541 gctgaagaag aagaagaaga ggaagcgcag ggacagtgat gcgcctggga aagaggggta
      601 cagggggggc ttgctgaagc tggaagccgc tgacccctac gtggagaccc ccacgagtcc
      661 caccttgcag gatatccccc aggctcccag cgacccctgc tcgggctggg actccgatac
      721 tccctcgagt ggatcttgtg ccactgtgtc acctgatcag gtcaaagaaa taaaaactga
      781 aggcaaacgg actatcgtcc ggcagggaaa gcaggtggtg ttccgagatg aggacagcac
      841 tggcaatgat gaggacatca tggtggactc agatgacgat tcctgggacc tcgtgacctg
      901 cttctgcatg aagccatttg ccggccgccc cgcgatcgag tgtaatgagt gccacacctg
      961 gattcacctg tcctgtgcga aaatccggaa atccaatgtt ccagaagtgt ttgtctgcca
     1021 aaagtgccgg gactccaagt ttgacatccg ccgttccaac cgctcgcgga cgggctcccg
     1081 gaagctgttc ctggactgac cgaattctgc agatatccag cacagtggcg gccgctcgag
     1141 tctagagggc ccgttaaccc ctccct
//
