LOCUS       sequence-483989-        1208 bp    DNA     linear   UNA 25-AUG-2025
DEFINITION  Expression of human PHF13 (W255A) with N terminal tet operator in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Rossi F, Magalhaes AP, Buschow R, Schubert T, Glaser L, Fontana A, 
            Mai J, Staege H, Grimme A, Will H, Schriener S, Hnisz D, Vingron M, 
            Chiariello AM, Kinkley S
  TITLE     Differential oligomerization regulates PHF13 chromatin affinity and 
            function.
  JOURNAL   Nucleic Acids Res. 2025 Jun 20;53(12):gkaf572. doi: 
            10.1093/nar/gkaf572.
  PUBMED    40598901
REFERENCE   2  (bases 1 to 1208)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 25, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2025-06-20"; volume: "53(12):gkaf572. doi"; 
            pages: " 10.1093/nar/gkaf572"
COMMENT     Sequence Label: pCDNA-4TO-PHF13W255A
FEATURES             Location/Qualifiers
     source          1..1208
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    20..38
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    41..59
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     69..93
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ggagctttag cagaagctct ccctatcagt gatagagatc tccctatcag tgatagagat
       61 cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      121 gacctccata gaagacaccg ggaccgatcc agcctccgga ctctagcgtt taaacttaag
      181 cttggtaccg agctcggatc catggactct gactcttgcg ccgccgcctt ccacccggag
      241 gaatactccc ccagttgcaa gaggcgcagg accgtggaag acttcaacaa attctgcacc
      301 tttgtcttgg cctatgctgg ctacatccct tatccgaagg aggaactccc tttaaggagc
      361 agccccagcc ctgctaacag cactgctggt accattgaca gcgacggctg ggacgcgggt
      421 ttctcagaca tcgcgtcctc agtgcccttg ccagtctctg accgctgctt tagccacctg
      481 cagcctactc tcttgcagcg agccaagccc agtaacttcc tgctggacag aaagaaaacg
      541 gacaagctga agaagaagaa gaagaggaag cgcagggaca gtgatgcgcc tgggaaagag
      601 gggtacaggg ggggcttgct gaagctggaa gccgctgacc cctacgtgga gacccccacg
      661 agtcccacct tgcaggatat cccccaggct cccagcgacc cctgctcggg ctgggactcc
      721 gatactccct cgagtggatc ttgtgccact gtgtcacctg atcaggtcaa agaaataaaa
      781 actgaaggca aacggactat cgtccggcag ggaaagcagg tggtgttccg agatgaggac
      841 agcactggca atgatgagga catcatggtg gactcagatg acgattcctg ggacctcgtg
      901 acctgcttct gcatgaagcc atttgccggc cgccccatga tcgagtgtaa tgagtgccac
      961 accgcgattc acctgtcctg tgcgaaaatc cggaaatcca atgttccaga agtgtttgtc
     1021 tgccaaaagt gccgggactc caagtttgac atccgccgtt ccaaccgctc gcggacgggc
     1081 tcccggaagc tgttcctgga ctgaccgaat tctgcagata tccagcacag tggcggccgc
     1141 tcgagtctag agggcccgtt taaacccgct gatcagcctc gacggtgcct tctagttgcc
     1201 agccatct
//
