LOCUS       sequence-483991-        1208 bp    DNA     linear   UNA 20-AUG-2025
DEFINITION  Expression of human PHF13 with N terminal deletion and N terminal 
            tet operator in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Rossi F, Magalhaes AP, Buschow R, Schubert T, Glaser L, Fontana A, 
            Mai J, Staege H, Grimme A, Will H, Schriener S, Hnisz D, Vingron M, 
            Chiariello AM, Kinkley S
  TITLE     Differential oligomerization regulates PHF13 chromatin affinity and 
            function.
  JOURNAL   Nucleic Acids Res. 2025 Jun 20;53(12):gkaf572. doi: 
            10.1093/nar/gkaf572.
  PUBMED    40598901
REFERENCE   2  (bases 1 to 1208)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 20, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2025-06-20"; volume: "53(12):gkaf572. doi"; 
            pages: " 10.1093/nar/gkaf572"
COMMENT     Sequence Label: pCDNA-4TO-GAL4-DD-PHF13∆NTD
FEATURES             Location/Qualifiers
     source          1..1208
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    14..32
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    35..53
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     63..87
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 tttagcagag ctctccctat cagtgataga gatctcccta tcagtgatag agatcgtcga
       61 cgagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
      121 catagaagac accgggaccg atccagcctc cggactctag cgtttaaact taagcttggt
      181 accatgacta gggcacatct gacagaagtg gaatcaaggc tagaaagact ggaacagcta
      241 tttctactga tttttcctcg agaagacctt gacatgattt tgaaaatgga ttctttacag
      301 gatataaaag cattgttaac aggattattt gtacaagata atgtgaataa agatgccgtc
      361 acagatagat tggcttcagt ggagactgat atgcctctaa cattgagaca gcatagaata
      421 agtgcgacat catcatcgga agagagtagt aacaaaggtc aaagacagtt gactgtatcg
      481 cggaattccc ggggatccat ggactctgac tcttgcgccg ccgccttcca cccggaggaa
      541 tactccccca gttgcgagga cgcgggtttc tcagacatcg cgtcctcagt gcccttgcca
      601 gtctctgacc gctgctttag ccacctgcag cctactctct tgcagcgagc caagcccagt
      661 aacttcctgc tggacagaaa gaaaacggac aagctgaaga agaagaagaa gaggaagcgc
      721 agggacagtg atgcgcctgg gaaagagggg tacagggggg gcttgctgaa gctggaagcc
      781 gctgacccct acgtggagac ccccacgagt cccaccttgc aggatatccc ccaggctccc
      841 agcgacccct gctcgggctg ggactccgat actccctcga gtggatcttg tgccactgtg
      901 tcacctgatc aggtcaaaga aataaaaact gaaggcaaac ggactatcgt ccggcaggga
      961 aagcaggtgg tgttccgaga tgaggacagc actggcaatg atgaggacat catggtggac
     1021 tcagatgacg attcctggga cctcgtgacc tgcttctgca tgaagccatt tgccggccgc
     1081 cccatgatcg agtgtaatga gtgcccacct ggattcacct gtcctgtgcg aaatccggaa
     1141 atccatgtcc cagaagggtt gtctgccaaa gtgccgggac tccagtttga catccgccgt
     1201 tcaaccgc
//
