LOCUS       sequence-483996-        8677 bp    DNA     circular SYN 20-AUG-2025
DEFINITION  Express doxycycline inducible UCE-containing intron of CRNDE in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8677)
  AUTHORS   Lee JS, Dan T, Zhang H, Cheng Y, Rehfeld F, Brugarolas J, Mendell JT
  TITLE     An ultraconserved snoRNA-like element in long noncoding RNA CRNDE 
            promotes ribosome biogenesis and cell proliferation.
  JOURNAL   Mol Cell. 2025 Apr 17;85(8):1543-1560.e10. doi: 
            10.1016/j.molcel.2025.03.006. Epub 2025 Apr 3.
  PUBMED    40185099
REFERENCE   2  (bases 1 to 8677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 20, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2025.03.006"; journalName: "Mol Cell"; date: 
            "2025-04-17- 17"; volume: "85"; issue: "8"; pages: "1543-1560.e10"
COMMENT     Sequence Label: XLone-UCE intron
FEATURES             Location/Qualifiers
     source          1..8677
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        9..376
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    17..35
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    53..71
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    89..107
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    125..143
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    161..179
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    197..215
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    233..251
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             442..450
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    3639..3773
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   4049..4083
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(4094..4111)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4094..4110)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4103..4125)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4319..4338)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4438..4460
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4498..4516)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4584..4688
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4689..4757,4758..5549)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4907..4926)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5720..6308
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6209..6228
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6462..6479
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6596..6617
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6632..6637,6638..6655,6656..6662)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6670..6686
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6675..6697
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6694..6710
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6694..6710
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   6736..6798
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    6974..7055
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(7172..7570)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             complement(7571..7627)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(7637..8380)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(8381..8404)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      8404..8413
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(8433..8644)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 atcacctcga gtttactccc tatcagtgat agagaacgta tgaagagttt actccctatc
       61 agtgatagag aacgtatgca gactttactc cctatcagtg atagagaacg tataaggagt
      121 ttactcccta tcagtgatag agaacgtatg accagtttac tccctatcag tgatagagaa
      181 cgtatctaca gtttactccc tatcagtgat agagaacgta tatccagttt actccctatc
      241 agtgatagag aacgtataag ctttgcttat gtaaaccagg gcgcctataa aagagtgctg
      301 attttttgag taaacttcaa ttccacaaca cttttgtctt ataccaactt tccgtaccac
      361 ttcctaccct cgtaaaggta ccagtctgca attcataatg gagctactgt actggctatt
      421 ggaaggagga gattctgaag ataaggaggt aatattatct cttttaaaag aatactttcc
      481 tctgtaatcc tgaatcttta ttacatgtaa gaactttgtg cagtagacag caatttcttt
      541 gaatttggta tatggaaaca attttatttt cctctgctaa gtttttgagc ctgcctcttc
      601 tagtgccatg gactgcattg gtagagctga gaaatatcat ttagccatac tcagcaccct
      661 taaaatagct tctttctgag aattagatct gtgaaggtgt cctgcacagt tcttgtagat
      721 gtcattttag tttgtggttg acgtgcatgc atttagcatg ttgcttaacc gtcctcattc
      781 gcctcccagt tctttgttgc cttcatttgg ggggatgtgt tttctgctgg atgatttact
      841 gctagacatg accaaactct gagtataaga cttggtgttt ggcagctggt tccagtgctc
      901 tgctgagaag tagttgggcc cagcctgggg cattgtagtg tcctggaggc cgggagctcc
      961 tgcacagggg ttcttttcct ggttcagagc cttaggtgcc tttccacttt tcacgtaatc
     1021 ttttcctagg ttggcttgct gcttactttg cagctgttgc agggattcac atggaatgga
     1081 gggctccctt ttattgtgga ttatttcttt gataatttac ccatgtgctt tttcattttc
     1141 aaaaacccag tggtgtttaa gaatgaaaga ttcttgaaga acaaaaattg ggttaaattt
     1201 gtatctatca gaaaagattc tatcctctga tgctatgtag gcacattgaa tatgcaggct
     1261 aaattaaaaa cagagtaaaa cctttttata atatgccaat tactatatct aagaatgttt
     1321 atacaggctg aaattttgta atggtattta tatctgtttt cctttttaag gaaaataata
     1381 tacttttcta ggcatcaaaa atatctgccc ctttcactaa tgctgatgta ccaccttccc
     1441 ccaaccccca acgctaaatt tgactggctt aaaaacatct gccccctgaa ctatatctgg
     1501 ggaggaatat taataaaaca atgaaggact tcatggtgtc tagttatata attaggaaat
     1561 tggttagaaa cgtggctaaa accagtttct ttcattaaaa gaactagtgt acatttaaaa
     1621 acaaaaacct tcaggaaaaa gactctttga tctttaagtc aaatagtatt tttggttaac
     1681 acagcaggaa ggggaaatat accaatttca gattctttta tttatgccta agtagaggtt
     1741 gtaagcagct aaggaagtga ttaaaatgta gtctagtaaa aaatggtgct gattacttgg
     1801 aaagcagttt acatttgcaa aaaaattcag tattatgacc ttcaccaact tttacacatc
     1861 atatacttgg gttattactt atggtatagc ttgtgtatgg aatgcaaggg tattttcaaa
     1921 ttgactaggt cttgctggtc atcttaaaat atgttacaat attacaaaat tgaaatgtaa
     1981 tattttttaa tagaaggaaa atataaattt aatatctggg caattgagac ctttaaactt
     2041 actttaaaag tatgatcttg atgtatatga tactgttttg tctttgctat attaacagaa
     2101 ttagaggggt gttctgcaat tcaaatacct tatatattcc aaattttatt ctctataatg
     2161 gacttttaaa ataaaaggta tatgtgcttc aagagggcaa aatttgaatc atgagctaat
     2221 ttgctaagca tcagattata gaaaagcatc cttgattaat ttggaactgt gaaagggggc
     2281 gggtaaaact gttttctgca gaaatttact agtgcagcaa ccatttaaat taaatgtttg
     2341 ttaacataat agtgatggca ttttctcctc cccctccttg tggttttgtc caactagatg
     2401 ttacagtggc agttgcactg actgttaagt gtttaaatga tgacaccatt atgtgaagtg
     2461 attttgaaat gagagattcc agccaagaat tacatctgct cccatctcct tcaaatcata
     2521 ctctctggca gtacagatta tgattgattt gtttgtgaca gattgcagga aacagtcatt
     2581 gatttttcaa tattttacct taaaattatt tacagttgta accatgggga ggtattttca
     2641 tgggctgtca gcccctgaaa gactaggata atattccctg ctctctgaca agacaaatta
     2701 cctgtaatga gtgcagtagc tgaagggtat acttttattt taaaatatgt caataacccc
     2761 agtgactaaa cgaatattga tttagcataa tgaagcctga gtaacgtgaa aatgagcttt
     2821 ttcaaggggc atggtaaagt ctttcttttt agctggttgt aagaagcttt tgattctttt
     2881 cagccagctg gtaggaatat agaattttat aagcaaacca tcaggaatga tagtgttgtt
     2941 tctgataagc aacatccaaa tattttgacc ctgcttttag tggttttttt caaatcttat
     3001 tttgagtctt acttttagtc atagaatagc tactgatttg atgcggtctt taactgactt
     3061 aatattttta caatttcaat atattttgca ttggaatctc cagtaatgaa tattaaaata
     3121 tatgtacaat catttgtaga tgatatcaat tatattaaga catttcagat gggctattgt
     3181 agtatttaat gtgccgtatt ttatggtaga ataattctca gtctctggac atcaagattg
     3241 ctttcagtgg gaatgaagat taatttactt cagtcctgat tttttaggca tcaatgcatg
     3301 ttttcatttt tgtcagactt ttaccctctt ttaatgtaat tctcaacttc ttatggattt
     3361 acttcccaat acataaaatc cttcaaaaca agaatgataa taatttttat actttttata
     3421 aaaataaatt tatttttagt ccatcaaggt gtctgaagac tagtagacca cctcccctgc
     3481 gagctaagct ggacagccaa tgacgggtaa gagagtgaca tttttcacta acctaagaca
     3541 ggagggccgt cagagctact gcctaatcca aagacgggta aaagtgataa aaatgtatca
     3601 ctccaaccta agacaggcgc agcttccgag ggatttgaga tccagacatg ataagataca
     3661 ttgatgagtt tggacaaacc aaaactagaa tgcagtgaaa aaaatgcctt atttgtgaaa
     3721 tttgtgatgc tattgcctta tttgtaacca ttataagctg caataaacaa gtttgatatc
     3781 tataacaaga aaatatatat ataataagtt atcacgtaag tagaacatga aataacaata
     3841 taattatcgt atgagttaaa tcttaaaagt cacgtaaaag ataatcatgc gtcattttga
     3901 ctcacgcggt cgttatagtt caaaatcagt gacacttacc gcattgacaa gcacgcctca
     3961 cgggagctcc aagcggcgac tgagatgtcc taaatgcaca gcgacggatt cgcgctattt
     4021 agaaagagag agcaatattt caagaatgca tgcgtcaatt ttacgcagac tatctttcta
     4081 gggttaagaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     4141 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     4201 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc
     4261 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
     4321 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     4381 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     4441 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
     4501 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     4561 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     4621 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     4681 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     4741 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     4801 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     4861 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     4921 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     4981 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     5041 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     5101 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     5161 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     5221 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     5281 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     5341 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     5401 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     5461 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     5521 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     5581 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     5641 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     5701 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     5761 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     5821 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     5881 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     5941 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     6001 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     6061 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     6121 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     6181 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     6241 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     6301 tatggaaaaa cgccagcaac gcggcctttt tacggtttct ggccttttgc tggccttttg
     6361 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     6421 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     6481 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     6541 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     6601 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     6661 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     6721 ccaaggtcga cttaacccta gaaagataat catattgtga cgtacgttaa agataatcat
     6781 gcgtaaaatt gacgcatgtg ttttatcggt ctgtatatcg aggtttattt attaatttga
     6841 atagatatta agttttatta tatttacact tacatactaa taataaattc aacaaacaat
     6901 ttatttatgt ttatttattt attaaaaaaa aacaaaaact caaaatttct tctataaagt
     6961 aacaaaactt ttagcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     7021 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
     7081 tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat
     7141 gtggtatggc tgattatgat cctctggaga tttagccctc ccacacataa ccagagggca
     7201 gcaattcacg aatcccaact gccgtcggct gtccatcact gtccttcact atggctttga
     7261 tcccaggatg cagatcgaga agcacctgtc ggcaccgtcc gcaggggctc aagatgcccc
     7321 tgttctcatt tccgatcgcg acgatacaag tcaggttgcc agctgccgca gcagcagcag
     7381 tgcccagcac cacgagttct gcacaaggtc ccccagtaaa atgatataca ttgacaccag
     7441 tgaagatgcg gccgtcgcta gagagagctg cgctggcgac gctgtagtct tcagagatgg
     7501 ggatgctgtt gattgtagcc gttgctcttt caatgagggt ggattcttct tgagacaaag
     7561 gcttggccat ggggccgggg ttctcctcca cgtcgccggc ctgcttcagc aggctgaagt
     7621 tggtggcgcc gctgcccccg gggagcatgt caaggtcaaa atcgtcaaga gcgtcagcag
     7681 gcagcatatc aaggtcaaag tcgtcaaggg catcggctgg gagcatgtct aagtcaaaat
     7741 cgtcaagggc gtcggtcggc ccgccgcttt cgcactttag ctgtttctcc aggccacata
     7801 tgattagttc caggccgaaa aggaaggcag gttcggctcc ctgccggtcg aacagctcaa
     7861 ttgcttgttt cagaagtggg ggcatagaat cggtggtagg tgtctctctt tcctcttttg
     7921 ctacttgatg ctcctgttcc tccaatacgc agcccagtgt aaagtggccc acggcggaca
     7981 gagcgtacag tgcgttctcc agggagaagc cttgctgaca caggaacgcg agctgatttt
     8041 ccagggtttc gtactgtttc tctgttgggc gggtgccgag atgcacttta gccccgtcgc
     8101 gatgtgagag gagagcacag cggtatgact tggcgttgtt ccgcagaaag tcttgccatg
     8161 actcgccttc cagggggcag gagtgggtat gatgcctgtc cagcatctcg attggcaggg
     8221 catcgagcag ggcccgcttg ttcttcacgt gccagtacag ggtaggctgc tcaactccca
     8281 gcttttgagc gagtttcctt gtcgtcaggc cttcgatacc gactccattg agtaattcca
     8341 gagcagagtt tatgactttg ctcttgtcca gtctagacat cttatcgtca tcgtctttgt
     8401 aatccatggt ggcggatccc gcgtcacgac acctgtgttc tggcggcaaa cccgttgcga
     8461 aaaagaacgt tcacggcgac tactgcactt atatacggtt ctcccccacc ctcgggaaaa
     8521 aggcggagcc agtacacgac atcactttcc cagtttaccc cgcgccacct tctctaggca
     8581 ccggttcaat tgccgacccc tccccccaac ttctcgggga ctgtgggcga tgtgcgctct
     8641 gcccactgac gggcaccgga gccactcgag tggaatt
//
