LOCUS       sequence-484000-       10511 bp    DNA     circular SYN 20-AUG-2025
DEFINITION  Express doxycycline inducible 5 kb UCE-containing CRNDE in mammalian
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10511)
  AUTHORS   Lee JS, Dan T, Zhang H, Cheng Y, Rehfeld F, Brugarolas J, Mendell JT
  TITLE     An ultraconserved snoRNA-like element in long noncoding RNA CRNDE 
            promotes ribosome biogenesis and cell proliferation.
  JOURNAL   Mol Cell. 2025 Apr 17;85(8):1543-1560.e10. doi: 
            10.1016/j.molcel.2025.03.006. Epub 2025 Apr 3.
  PUBMED    40185099
REFERENCE   2  (bases 1 to 10511)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 20, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2025.03.006"; journalName: "Mol Cell"; date: 
            "2025-04-17- 17"; volume: "85"; issue: "8"; pages: "1543-1560.e10"
COMMENT     Sequence Label: XLone-CRNDEUCE (5 kb)
FEATURES             Location/Qualifiers
     source          1..10511
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        9..376
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    17..35
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    53..71
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    89..107
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    125..143
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    161..179
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    197..215
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    233..251
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             621..629
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    5473..5607
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   5883..5917
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5928..5945)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5928..5944)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5937..5959)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(6153..6172)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6272..6294
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6332..6350)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6418..6522
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6523..6591,6592..7383)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6741..6760)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7554..8142
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8043..8062
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8296..8313
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8430..8451
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8466..8471,8472..8489,8490..8496)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8504..8520
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8509..8531
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8528..8544
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8528..8544
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   8570..8632
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    8808..8889
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(9006..9404)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             complement(9405..9461)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(9471..10214)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(10215..10238)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      10238..10247
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(10267..10478)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 atcacctcga gtttactccc tatcagtgat agagaacgta tgaagagttt actccctatc
       61 agtgatagag aacgtatgca gactttactc cctatcagtg atagagaacg tataaggagt
      121 ttactcccta tcagtgatag agaacgtatg accagtttac tccctatcag tgatagagaa
      181 cgtatctaca gtttactccc tatcagtgat agagaacgta tatccagttt actccctatc
      241 agtgatagag aacgtataag ctttgcttat gtaaaccagg gcgcctataa aagagtgctg
      301 attttttgag taaacttcaa ttccacaaca cttttgtctt ataccaactt tccgtaccac
      361 ttcctaccct cgtaaaggta ccgccgcagc cgcagcccct ggcgctaacg gtcggtaacg
      421 gcccgcgcgc ccgcgcggcg gaggagaggt gttaagtgtg atgcttccat aatacatttg
      481 gatgctgtca gctaagttca cttctgaact aaggggttcc tccaaatgtt ggctgaaatt
      541 catcccaagg ctggtctgca aagtctgcaa ttcataatgg agctactgta ctggctattg
      601 gaaggaggag attctgaaga taaggaggta atattatctc ttttaaaaga atactttcct
      661 ctgtaatcct gaatctttat tacatgtaag aactttgtgc agtagacagc aatttctttg
      721 aatttggtat atggaaacaa ttttattttc ctctgctaag tttttgagcc tgcctcttct
      781 agtgccatgg actgcattgg tagagctgag aaatatcatt tagccatact cagcaccctt
      841 aaaatagctt ctttctgaga attagatctg tgaaggtgtc ctgcacagtt cttgtagatg
      901 tcattttagt ttgtggttga cgtgcatgca tttagcatgt tgcttaaccg tcctcattcg
      961 cctcccagtt ctttgttgcc ttcatttggg gggatgtgtt ttctgctgga tgatttactg
     1021 ctagacatga ccaaactctg agtataagac ttggtgtttg gcagctggtt ccagtgctct
     1081 gctgagaagt agttgggccc agcctggggc attgtagtgt cctggaggcc gggagctcct
     1141 gcacaggggt tcttttcctg gttcagagcc ttaggtgcct ttccactttt cacgtaatct
     1201 tttcctaggt tggcttgctg cttactttgc agctgttgca gggattcaca tggaatggag
     1261 ggctcccttt tattgtggat tatttctttg ataatttacc catgtgcttt ttcattttca
     1321 aaaacccagt ggtgtttaag aatgaaagat tcttgaagaa caaaaattgg gttaaatttg
     1381 tatctatcag aaaagattct atcctctgat gctatgtagg cacattgaat atgcaggcta
     1441 aattaaaaac agagtaaaac ctttttataa tatgccaatt actatatcta agaatgttta
     1501 tacaggctga aattttgtaa tggtatttat atctgttttc ctttttaagg aaaataatat
     1561 acttttctag gcatcaaaaa tatctgcccc tttcactaat gctgatgtac caccttcccc
     1621 caacccccaa cgctaaattt gactggctta aaaacatctg ccccctgaac tatatctggg
     1681 gaggaatatt aataaaacaa tgaaggactt catggtgtct agttatataa ttaggaaatt
     1741 ggttagaaac gtggctaaaa ccagtttctt tcattaaaag aactagtgta catttaaaaa
     1801 caaaaacctt caggaaaaag actctttgat ctttaagtca aatagtattt ttggttaaca
     1861 cagcaggaag gggaaatata ccaatttcag attcttttat ttatgcctaa gtagaggttg
     1921 taagcagcta aggaagtgat taaaatgtag tctagtaaaa aatggtgctg attacttgga
     1981 aagcagttta catttgcaaa aaaattcagt attatgacct tcaccaactt ttacacatca
     2041 tatacttggg ttattactta tggtatagct tgtgtatgga atgcaagggt attttcaaat
     2101 tgactaggtc ttgctggtca tcttaaaata tgttacaata ttacaaaatt gaaatgtaat
     2161 attttttaat agaaggaaaa tataaattta atatctgggc aattgagacc tttaaactta
     2221 ctttaaaagt atgatcttga tgtatatgat actgttttgt ctttgctata ttaacagaat
     2281 tagaggggtg ttctgcaatt caaatacctt atatattcca aattttattc tctataatgg
     2341 acttttaaaa taaaaggtat atgtgcttca agagggcaaa atttgaatca tgagctaatt
     2401 tgctaagcat cagattatag aaaagcatcc ttgattaatt tggaactgtg aaagggggcg
     2461 ggtaaaactg ttttctgcag aaatttacta gtgcagcaac catttaaatt aaatgtttgt
     2521 taacataata gtgatggcat tttctcctcc ccctccttgt ggttttgtcc aactagatgt
     2581 tacagtggca gttgcactga ctgttaagtg tttaaatgat gacaccatta tgtgaagtga
     2641 ttttgaaatg agagattcca gccaagaatt acatctgctc ccatctcctt caaatcatac
     2701 tctctggcag tacagattat gattgatttg tttgtgacag attgcaggaa acagtcattg
     2761 atttttcaat attttacctt aaaattattt acagttgtaa ccatggggag gtattttcat
     2821 gggctgtcag cccctgaaag actaggataa tattccctgc tctctgacaa gacaaattac
     2881 ctgtaatgag tgcagtagct gaagggtata cttttatttt aaaatatgtc aataacccca
     2941 gtgactaaac gaatattgat ttagcataat gaagcctgag taacgtgaaa atgagctttt
     3001 tcaaggggca tggtaaagtc tttcttttta gctggttgta agaagctttt gattcttttc
     3061 agccagctgg taggaatata gaattttata agcaaaccat caggaatgat agtgttgttt
     3121 ctgataagca acatccaaat attttgaccc tgcttttagt ggtttttttc aaatcttatt
     3181 ttgagtctta cttttagtca tagaatagct actgatttga tgcggtcttt aactgactta
     3241 atatttttac aatttcaata tattttgcat tggaatctcc agtaatgaat attaaaatat
     3301 atgtacaatc atttgtagat gatatcaatt atattaagac atttcagatg ggctattgta
     3361 gtatttaatg tgccgtattt tatggtagaa taattctcag tctctggaca tcaagattgc
     3421 tttcagtggg aatgaagatt aatttacttc agtcctgatt ttttaggcat caatgcatgt
     3481 tttcattttt gtcagacttt taccctcttt taatgtaatt ctcaacttct tatggattta
     3541 cttcccaata cataaaatcc ttcaaaacaa gaatgataat aatttttata ctttttataa
     3601 aaataaattt atttttagtc catcaaggtg tctgaagatt ttatgcctag gtatctccat
     3661 atctaacttg ataaggaaaa taggataaac aatgctggta atagcaggaa agtaagtatt
     3721 tgaataagat gtcaaactga tatttcatgt gaacctaact cattttatgg taactaataa
     3781 ttatcttatt taaatcaata ggtaaaacct gtttagaaat taaaaatgag ttacgattta
     3841 aagaaaattc agatgactca ttgtgagtgc tagttctctt gtaggatgcc actggaaatg
     3901 ttgaaatgaa aaatgtaagt atatcttttg gtggaaaaaa ggatagtctc taggacacaa
     3961 aattactgtt ttattttttt ctcaggagtt tgcctaaggg tgtgacagat gatctctgtc
     4021 acttgtctta gttgtgtcct gcaataaact ggatgcttta taaaatacta gacctgtgat
     4081 ttcgtatgct gtaatatttc atttctccat cacccctcca aattatttct tagtttggag
     4141 taaaataata aatgtattat agtcaacatc tcttgacccc tctttagttt cagctaaact
     4201 aagcatgtgt gtttgtgtgt tcattttata gttcatgtgt agaactatgt gaattaaatt
     4261 taagaaacat gtaaagtaga ggaaatagtt ttctggagaa atttttcctt tttggatatt
     4321 atgccctttt ccattgcttt tctctgcttg aaagcaaaaa aaagtaccct acccctgttc
     4381 tcctttaggg aaaaactatt cctataaagt atttttaaat cgtgcaagtc attgcctagg
     4441 gttagctaaa acatttcttt ttaaaaagga gaaaatgccc tggctttaac attttcttgt
     4501 atttgtatct attaagataa acagtttact ttgatacagt acataccaat ctacttaatt
     4561 ttttttccag gattcctttt actatgtttg gtctgacctt ttatgataac ttaatatggg
     4621 aacaaattag catataattc tattttccat gtgacctcaa ccagttgcag aattgtacca
     4681 ctactttagg gggggcaatt tgacagttta tgtagactat agcattaatt gttcccaaat
     4741 gttcagtgca tcctggctaa tgtgttattg aaggtgtttt cacgtaagca gttagaggaa
     4801 gcacttcacc cctattacta agttattaaa atgcctccta aaggtagcat tttaaattag
     4861 tatacataat tgattagtaa tttgtcttct cccaagcata aaacagcata gcagagttaa
     4921 gtgtgaccag tgaagtataa gatattaggg attgatggtg acaatgatca tagcaactaa
     4981 atggattttt tttttctttt agattcagcc gttggtcttt gaaatttcct gtgatgtgtt
     5041 tcaatctaga tgcaaagaac atggaaaaat caaagtgctc gagtggttta aatatgtttt
     5101 gggtattcct gtttatagac tataatactt ttccaattaa aatcctcagt tgtcacgcag
     5161 aagaaggtta agctgtattt gattgccagt tttactgaaa atgcttagta ttttacagta
     5221 tcaccaaata tattttgttt agccaaggta taggaaaaat aaaataaatt gtataggttg
     5281 acttttttct aaactagtag accacctccc ctgcgagcta agctggacag ccaatgacgg
     5341 gtaagagagt gacatttttc actaacctaa gacaggaggg ccgtcagagc tactgcctaa
     5401 tccaaagacg ggtaaaagtg ataaaaatgt atcactccaa cctaagacag gcgcagcttc
     5461 cgagggattt gagatccaga catgataaga tacattgatg agtttggaca aaccaaaact
     5521 agaatgcagt gaaaaaaatg ccttatttgt gaaatttgtg atgctattgc cttatttgta
     5581 accattataa gctgcaataa acaagtttga tatctataac aagaaaatat atatataata
     5641 agttatcacg taagtagaac atgaaataac aatataatta tcgtatgagt taaatcttaa
     5701 aagtcacgta aaagataatc atgcgtcatt ttgactcacg cggtcgttat agttcaaaat
     5761 cagtgacact taccgcattg acaagcacgc ctcacgggag ctccaagcgg cgactgagat
     5821 gtcctaaatg cacagcgacg gattcgcgct atttagaaag agagagcaat atttcaagaa
     5881 tgcatgcgtc aattttacgc agactatctt tctagggtta agaattcact ggccgtcgtt
     5941 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     6001 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     6061 ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc
     6121 ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     6181 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     6241 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     6301 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
     6361 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     6421 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     6481 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     6541 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     6601 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     6661 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     6721 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     6781 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     6841 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     6901 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     6961 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     7021 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     7081 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     7141 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     7201 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     7261 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     7321 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     7381 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     7441 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     7501 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     7561 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     7621 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     7681 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     7741 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     7801 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     7861 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     7921 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     7981 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     8041 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     8101 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     8161 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     8221 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     8281 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     8341 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     8401 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     8461 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     8521 tttcacacag gaaacagcta tgaccatgat tacgccaagg tcgacttaac cctagaaaga
     8581 taatcatatt gtgacgtacg ttaaagataa tcatgcgtaa aattgacgca tgtgttttat
     8641 cggtctgtat atcgaggttt atttattaat ttgaatagat attaagtttt attatattta
     8701 cacttacata ctaataataa attcaacaaa caatttattt atgtttattt atttattaaa
     8761 aaaaaacaaa aactcaaaat ttcttctata aagtaacaaa acttttagca gtgaaaaaaa
     8821 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     8881 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
     8941 gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta tgatcctctg
     9001 gagatttagc cctcccacac ataaccagag ggcagcaatt cacgaatccc aactgccgtc
     9061 ggctgtccat cactgtcctt cactatggct ttgatcccag gatgcagatc gagaagcacc
     9121 tgtcggcacc gtccgcaggg gctcaagatg cccctgttct catttccgat cgcgacgata
     9181 caagtcaggt tgccagctgc cgcagcagca gcagtgccca gcaccacgag ttctgcacaa
     9241 ggtcccccag taaaatgata tacattgaca ccagtgaaga tgcggccgtc gctagagaga
     9301 gctgcgctgg cgacgctgta gtcttcagag atggggatgc tgttgattgt agccgttgct
     9361 ctttcaatga gggtggattc ttcttgagac aaaggcttgg ccatggggcc ggggttctcc
     9421 tccacgtcgc cggcctgctt cagcaggctg aagttggtgg cgccgctgcc cccggggagc
     9481 atgtcaaggt caaaatcgtc aagagcgtca gcaggcagca tatcaaggtc aaagtcgtca
     9541 agggcatcgg ctgggagcat gtctaagtca aaatcgtcaa gggcgtcggt cggcccgccg
     9601 ctttcgcact ttagctgttt ctccaggcca catatgatta gttccaggcc gaaaaggaag
     9661 gcaggttcgg ctccctgccg gtcgaacagc tcaattgctt gtttcagaag tgggggcata
     9721 gaatcggtgg taggtgtctc tctttcctct tttgctactt gatgctcctg ttcctccaat
     9781 acgcagccca gtgtaaagtg gcccacggcg gacagagcgt acagtgcgtt ctccagggag
     9841 aagccttgct gacacaggaa cgcgagctga ttttccaggg tttcgtactg tttctctgtt
     9901 gggcgggtgc cgagatgcac tttagccccg tcgcgatgtg agaggagagc acagcggtat
     9961 gacttggcgt tgttccgcag aaagtcttgc catgactcgc cttccagggg gcaggagtgg
    10021 gtatgatgcc tgtccagcat ctcgattggc agggcatcga gcagggcccg cttgttcttc
    10081 acgtgccagt acagggtagg ctgctcaact cccagctttt gagcgagttt ccttgtcgtc
    10141 aggccttcga taccgactcc attgagtaat tccagagcag agtttatgac tttgctcttg
    10201 tccagtctag acatcttatc gtcatcgtct ttgtaatcca tggtggcgga tcccgcgtca
    10261 cgacacctgt gttctggcgg caaacccgtt gcgaaaaaga acgttcacgg cgactactgc
    10321 acttatatac ggttctcccc caccctcggg aaaaaggcgg agccagtaca cgacatcact
    10381 ttcccagttt accccgcgcc accttctcta ggcaccggtt caattgccga cccctccccc
    10441 caacttctcg gggactgtgg gcgatgtgcg ctctgcccac tgacgggcac cggagccact
    10501 cgagtggaat t
//
