LOCUS       sequence-484260-        3600 bp    DNA     circular SYN 21-AUG-2025
DEFINITION  Retroviral vector containing human BAP1 and Thy1.1 selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Balasubramani A, Larjo A, Bassein JA, Chang X, Hastie RB, Togher SM,
            Lahdesmaki H, Rao A
  TITLE     Cancer-associated ASXL1 mutations may act as gain-of-function 
            mutations of the ASXL1-BAP1 complex.
  JOURNAL   Nat Commun. 2015 Jun 22;6:7307. doi: 10.1038/ncomms8307.
  PUBMED    26095772
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 21, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-06-22"; volume: "6:7307. doi"; pages: " 
            10.1038/ncomms8307"
COMMENT     Sequence Label: BAP1
FEATURES             Location/Qualifiers
     source          1..3600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(588..607)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(735..757)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(951..970)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1070..1092
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1130..1148)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1216..1320
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1321..1389,1390..2181)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1539..1558)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2352..2940
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2841..2860
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3094..3111
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3228..3249
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3264..3269,3270..3287,3288..3294)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3302..3318
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 atcggaggac tggcgcgccg agtgaggggt tgtgggctct tttattgagc tcggggagca
       61 gaagcgcgcg aacagaagcg agaagcgaac tgattggtta gttcaaataa ggcacagggt
      121 catttcaggt ccttggggca ccctggaaac atctgatggt tctctagaaa ctgctgaggg
      181 cgggaccgca tctggggacc atctgttctt ggccctgagc cggggcagga actgcttacc
      241 acagatatcc tgtttggccc atattctgct gtctctctgt tcctaacctt gatctgaact
      301 tctctattct cagttatgta ttttccatgc cttgcaaaat ggcgttactt aagctagctt
      361 gccaaaccta caggtggggt ctttcattcc cccctttttc tggagactaa ataaaatctt
      421 ttattttatc tatggctcgt actctatagg cttcagctgg tgatattgtt gagtcaaaac
      481 tagagcctgg accactgata tcctgtcttt aacaaattgg actaatcgcc tctacgccgg
      541 acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga
      601 catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt
      661 gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc
      721 attccttgcg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
      781 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
      841 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt
      901 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc
      961 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     1021 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     1081 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     1141 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     1201 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     1261 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     1321 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     1381 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     1441 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     1501 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     1561 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     1621 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     1681 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     1741 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     1801 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     1861 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     1921 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     1981 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     2041 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     2101 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     2161 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     2221 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     2281 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     2341 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     2401 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     2461 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     2521 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     2581 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     2641 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     2701 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     2761 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     2821 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     2881 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     2941 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     3001 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     3061 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     3121 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     3181 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     3241 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     3301 attgtgagcg gataacaatt tcacaattct ccccagctct ttctgcgctg tccataatga
     3361 actatttcag gagtattgga gtatgtggat tcaaaacagt ggctaattga gtcaggatta
     3421 cttaatattc gactcctacc ctcagaatgt tgttctccaa cttcaagaaa ctgttaccca
     3481 tgaaagaccc ccgctgacgg gtagtcaatc actcagagga gaccctccca aggaacagcg
     3541 agaccacaag tcggatgcaa ctgcaagagg gtttattgga tacacgggta cccgggcgac
//
