LOCUS       sequence-484267-       14191 bp    DNA     circular SYN 21-AUG-2025
DEFINITION  Mammalian expression of mCherry-Talin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14191)
  AUTHORS   Lee HS, Anekal P, Lim CJ, Liu CC, Ginsberg MH
  TITLE     Two modes of integrin activation form a binary molecular switch in 
            adhesion maturation.
  JOURNAL   Mol Biol Cell. 2013 May;24(9):1354-62. doi: 10.1091/mbc.E12-09-0695.
            Epub 2013 Mar 6.
  PUBMED    23468527
REFERENCE   2  (bases 1 to 14191)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 21, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E12-09-0695"; journalName: "Mol Biol Cell"; date: 
            "2013-05"; volume: "24"; issue: "9"; pages: "1354-62"
COMMENT     Sequence Label: pmCherry-Talin
FEATURES             Location/Qualifiers
     source          1..14191
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             689..1396
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(834..852)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1102..1121
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     regulatory      1412..1421
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(9489..9505)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9489..9505)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(9548..9565)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9554..9778
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9824..10252
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9911..9930)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10121..10142
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10261..10281)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10266..10595
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10446..10581
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10508..10527
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             10662..11456
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10716..10735)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11326..11345
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    11630..11751
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11667..11686)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11721..11740
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11800..11816)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11800..11816)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11813..11835)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11824..11840
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11848..11854,11855..11872,11873..11878))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11893..11914
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12031..12048)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12202..12790)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12282..12301)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12961..13752,13753..13821))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13584..13603
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13822..13926)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(14001..14020)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacttaagc ttggtaccat ggtgagcaag ggcgaggagg ataacatggc
      721 catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga
      781 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
      841 gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat
      901 gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc
      961 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac
     1021 cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg
     1081 caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
     1141 ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa
     1201 gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc
     1261 cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga
     1321 ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat
     1381 ggacgagctg tacaaggaat tcgcagaagc tgccaccatg gttgcgcttt cgctgaagat
     1441 tagcattggg aatgtggtga agacgatgca atttgagcca tctaccatgg tgtacgacgc
     1501 ctgccgcatg attcgtgagc ggatcccaga ggccctggct ggccctccca acgactttgg
     1561 gctctttctg tcagatgatg accccaaaaa aggcatctgg ctagaggctg ggaaagcttt
     1621 ggactactac atgctccgaa atggggacac catggagtac agaaaaaagc agagacccct
     1681 gaagatccgg atgttagacg gaacggtgaa gactatcatg gtggatgact ccaagaccgt
     1741 cacagacatg ctcatgacca tttgtgcccg aattggtatc accaaccatg atgagtattc
     1801 actggttcga gagctgatgg aagaaaagaa agatgagggg acagggaccc tgagaaaaga
     1861 caagacccta ctgcgagatg aaaagaagat ggagaagcta aagcagaagt tgcacacaga
     1921 cgatgagttg aactggctgg accatgggcg gacactgagg gaacagggag tggaagagca
     1981 tgagacgttg ctgctgcgga gaaagttctt ctactcagac cagaatgtgg attcccgaga
     2041 ccctgtacag ctgaaccttc tctatgtgca ggcacgagat gacatcctga atggctccca
     2101 tcctgtctcc tttgacaagg cctgtgaatt tgcaggcttc cagtgccaga tccagtttgg
     2161 acctcacaat gaacagaagc acaaggctgg cttccttgac ctgaaggact tcctgcccaa
     2221 ggagtatgtg aagcagaagg gagagcgtaa gatctttcag gcacacaaga attgtgggca
     2281 gatgagtgaa attgaggcca aggtacgcta cgtgaagctg gcccgttccc tcaagactta
     2341 cggtgtctcc ttcttcctag tcaaggaaaa gatgaagggg aagaataaac tggtgcccag
     2401 gctgttgggc atcactaagg agtgtgtgat gcgtgtggat gagaagacca aggaggtgat
     2461 tcaggaatgg agcctcacca acatcaaacg ctgggctgcc tctcccaaga gctttactct
     2521 ggactttgga gactatcagg atggctacta ctcagtacag acgacagaag gcgagcagat
     2581 cgcacagctc attgctggct acatagatat catccttaag aagaaaaaaa gcaaggacca
     2641 ttttgggctg gagggagatg aagagtctac tatgttggag gactcagtgt ctcccaaaaa
     2701 gtcaacagtc cttcagcagc agtacaaccg agtggggaaa gtggagcacg gctctgtggc
     2761 tctgccagcc atcatgcgct ctggagcctc tggtcctgag aatttccagg tggggagcat
     2821 gccacctgcc cagcagcaga tcaccagtgg ccagatgcac cgaggacaca tgccacctct
     2881 gacttcagcc cagcaggcgc tcactggaac cattaactcc agcatgcagg ctgtgcaggc
     2941 tgcccaggcc actctggatg actttgagac tctaccccct cttggccagg atgctgcctc
     3001 caaagcttgg cgtaagaaca agatggatga atcaaagcat gagatccact cccaggtaga
     3061 tgccatcaca gctggcactg cctctgtggt aaacctgaca gcaggagatc ctgcagagac
     3121 agactataca gcagtgggct gtgcagtcac caccatctct tccaacctga cggagatgtc
     3181 ccgtggggtg aagctattgg ctgccctgct ggaggatgaa ggcggcaatg gccggcccct
     3241 tctgcaagca gcaaagggcc ttgcaggggc tgtgtcggaa ctgcttcgca gtgctcagcc
     3301 cgccagcgct gagcctcgtc agaacctgct gcaggcagcc gggaacgtgg gccaggccag
     3361 tggggagctg ttgcagcaaa ttggggaaag tgacactgac ccccacttcc aggatgttct
     3421 aatgcagcta gcaaacgcag tggcaagtgc tgcagccgcc ctggtcctca aggccaagag
     3481 tgtggcccag cgcacagagg attccgggct tcagacccaa gtgattgctg cagctacgca
     3541 atgtgctctg tccacttccc agctggtggc ctgtaccaag gtggtagcac ctacaatcag
     3601 ctcacctgtg tgccaagaac agctagtcga ggctgggcga ctggtggcca aagctgtgga
     3661 gggctgtgtg tctgcctcgc aagcagctac agaagatgga cagctgcttc gaggggtagg
     3721 agcagcagcc acggctgtca cccaggccct caatgagctg ctgcagcacg tgaaggccca
     3781 cgccaccgga gctgggcctg ccggtcggta tgaccaagcc actgatacca tcctcaccgt
     3841 cactgaaaac atcttcagct ccatgggtga tgctggggag atggtgcgcc aggcccgcat
     3901 cctggcccaa gccacctcag acctggtcaa tgctatcaag gccgatgctg agggggagag
     3961 tgatctggag aactctagaa agctcctgag tgctgccaag atcctcgctg atgccaccgc
     4021 caagatggtg gaggcggcca agggagcagc cgcccaccct gacagtgagg aacagcagca
     4081 gcgactgcgt gaagcagctg aggggcttcg catggccacc aatgcagctg cgcagaacgc
     4141 catcaagaag aagttggtgc agcgcctgga gcatgcagcc aagcaagctg cagcctctgc
     4201 aacacagacc attgctgcag cccaacatgc agcctctgcc cccaaggcct ctgcgggccc
     4261 ccagccccta ctggtgcaga gctgtaaggc cgtggcagag cagattccgt tgctggtgca
     4321 gggtgtccga ggaagtcaag ctcagcctga cagccctagt gctcagctcg ccctcattgc
     4381 tgccagccag agcttcctgc agccaggtgg gaagatggtg gcagcggcaa aagcctcagt
     4441 gccaacaatt caggaccagg cttcagcgat gcagctgagt cagtgcgcaa agaacctagg
     4501 cactgccctg gccgagctgc gcactgctgc tcagaaggct caggaggcat gtggaccttt
     4561 ggagatggat tctgcactga gtgttgtaca aaatctagag aaagacctac aggaaataaa
     4621 agcagcagct cgagatggca agcttaagcc cttacctggg gaaacaatgg agaagtgtac
     4681 ccaagatctt ggcaacagca ccaaagcggt gagctctgcc atcgccaagc tgctggggga
     4741 gattgcccag ggcaatgaga actatgcagg tattgcagct cgggatgtag ctggtggact
     4801 aaggtcacta gcccaggctg cgcggggtgt ggctgcgctg acatcagatc ctgcagtgca
     4861 ggccattgtg cttgacacag ccagcgacgt tctggacaag gccagcagcc tcattgaaga
     4921 ggcaaaaaaa gcatctggac acccagggga cccagaaagc cagcaaaggc ttgctcaggt
     4981 agctaaagca gtgacccaag ccctgaaccg ctgtgtcagc tgcctgcctg gccaaagaga
     5041 cgtggataat gccctacgag ccgttggaga tgccagcaag cgactcctga gtgacttgct
     5101 tcctccaagc acggggacat ttcaagaagc acagagccga ttgaatgaag ctgcagctgg
     5161 gttaaatcag gccgccacag aactggtgca agcctcccga ggaactcctc aggacctggc
     5221 tcgggcctcc ggccgatttg gacaggactt cagcaccttc ctggaagctg gtgtggagat
     5281 ggccggacag gccccgagcc aggaggaccg agcccaggtg gtgtccaacc tgaagggcat
     5341 atccatgtct tcaagcaaac ttctccttgc cgctaaggcc ttgtccacag accctgcttc
     5401 tcccaacctc aagagtcagc tggctgcggc tgcccgggca gtgacggaca gcatcaacca
     5461 gctcatcacc atgtgcaccc agcaggcacc tggccagaag gagtgtgaca atgcacttcg
     5521 gcagctagag acagtccgag aactcctgga gaatccagtc cagcccatca acgacatgtc
     5581 ctacttcggt tgcttggaca gtgtcatgga gaactccaag gtcctaggtg aggccatgac
     5641 tggcatctcc caaaatgcca agaatggaaa tctgccggag tttggagatg ccattgccac
     5701 agcctccaag gctctctgtg gcttcacgga ggcagccgca caggcagcat atctagttgg
     5761 tgtctctgac cccaacagcc aagctggaca gcaaggactg gtggaaccca cacagtttgc
     5821 ccgtgcaaac caggcaattc agatggcctg tcagagtctg ggggagcctg gctgtaccca
     5881 ggcccaggtg ttatctgcag ccactattgt agccaaacac acatctgcat tgtgtaacag
     5941 ctgtcgcctg gcttccgcta ggactgccaa tcccactgcc aagcgccagt ttgtacagtc
     6001 agccaaggag gtggccaaca gtacagccaa tcttgtcaag accatcaagg cactagatgg
     6061 ggacttcaca gaagagaacc gtgcccagtg ccgagcagcc acagcccctc tgctggaagc
     6121 tgtggataac ctgagtgcct ttgcctccaa ccctgagttc tccagcgtcc ctgcccagat
     6181 cagccctgag ggcagagcag ccatggagcc cattgtaatc tctgctaaga caatgttgga
     6241 gagtgctgga gggctcatcc agacagctcg ggcgttagca gtcaatcccc gagacccccc
     6301 acgttggtct gtgctagctg gccactctcg gactgtctca gattccatca agaaacttat
     6361 tacaagcatg agggacaaag ccccagggca gctggagtgt gagacagcca ttgcggctct
     6421 gaacagctgc ttgcgggacc tagatcaggc ttcgcttgcc gctgtcagcc agcagcttgc
     6481 tccccgtgaa ggaatctctc aagaggcttt gcacacccag atgctcactg cagtgcaaga
     6541 aatttctcat ctcattgagc cgctggccag cgctgctcgg gctgaagcct cccagctggg
     6601 acacaaggtg tcccaaatgg cccagtactt tgaaccactc accctggctg cagtgggtgc
     6661 tgcgtctaag accctgagcc acccgcagca gatggcactt ctggaccaga ctaaaacgtt
     6721 ggcagagtct gccttgcagt tgctatatac tgccaaggag gctggtggta accccaagca
     6781 agcagcacac acccaggaag ccctggagga ggctgtgcag atgatgacag aggccgtaga
     6841 agacctgacg acgaccctca acgaagcagc cagtgctgca ggagtcgttg gcggcatggt
     6901 ggactctatc actcaggcca tcaaccagct agatgaagga cctatgggtg acccagaagg
     6961 ctcattcgta gattaccaga caaccatggt gaggacagcc aaggccattg ctgtcactgt
     7021 tcaggagatg gtaaccaagt caaacaccag ccctgaagag ctaggccctc ttgccaacca
     7081 gctgaccagt gactatggcc gactggcctc acaagccaag cctgcagctg tggctgctga
     7141 aaatgaagag ataggcgctc acatcaaaca ccgagtacag gagctgggcc atggctgctc
     7201 tgctctggtc accaaggcag gtgccttgca gtgtagcccc agtgatgtct acaccaagaa
     7261 ggagctcata gagtgtgccc gcagagtgtc agagaaggtc tcccatgttc tggctgcact
     7321 ccaggctggg aatcgtggta cccaggcctg cattacagca gccagtgctg tgtctggtat
     7381 cattgctgac ctcgacacca ccatcatgtt cgctactgct ggcacactta accgtgaggg
     7441 tgctgaaact tttgctgacc accgggaggg tatcttaaag acagcaaagg ttcttgtgga
     7501 ggacaccaag gtcctagtgc agaatgcagc tgggagccag gagaagttgg cacaagccgc
     7561 ccagtcctcc gtggccacca ttacccgcct cgctgatgtg gtcaagctcg gtgcagccag
     7621 cctaggagcc gaagaccctg aaactcaggt ggtgctgatc aatgcagtaa aggacgtagc
     7681 caaggccctg ggtgacctca tcagcgctac gaaggctgca gcgggcaaag ttggggatga
     7741 ccctgcagtg tggcagctca agaactctgc caaggtgatg gtgaccaatg tgacatcatt
     7801 gctcaagaca gtgaaggctg tggaagatga ggccaccaaa ggcacacggg ccctagaggc
     7861 aaccacagag cacatacgtc aggaactggc ggtcttctgt tccccagagc cacctgccaa
     7921 gacctctacc cctgaagact tcatccgaat gaccaagggt attactatgg caacagccaa
     7981 agccgttgct gctggcaatt cctgtcgaca ggaagatgtc attgccacag ccaatctgag
     8041 ccgacgggct attgcggaca tgcttcgggc ttgcaaggaa gcagctttcc acccagaagt
     8101 ggcgcctgat gtacggctcc gagccctgca ctatggccgg gagtgtgcca atggttacct
     8161 ggaactgctg gaccacgtgc tgctgaccct tcagaagcca aacccagacc tgaagcagca
     8221 gctaactgga cactcaaagc gggttgctgg ctctgtgact gagctcatcc aggctgctga
     8281 agccatgaaa ggaacagagt gggtggaccc agaggaccct actgtcattg ctgagaatga
     8341 gctcttggga gccgcagccg ccatcgaggc tgcagccaag aagctggagc agctgaagcc
     8401 cagggccaaa cccaaggagg cggatgagtc cttgaacttt gaggaacaaa tcctagaagc
     8461 tgccaagtcc atcgctgcag ccaccagtgc actggtaaag gctgcgtcag cagcccagag
     8521 ggagctggtg gctcaaggaa aggtgggcgc cattccagcc aatgcactgg atgatgggca
     8581 gtggtcgcag ggcctcattt ccgctgcccg tatggtggct gcggccacca acaatctgtg
     8641 tgaagcagct aacgcagctg tccagggcca tgctagccag gagaaactca tttcctcagc
     8701 caagcaagta gctgcctcca cagctcagct cctggtagct tgcaaggtca aagcagatca
     8761 ggactctgag gcaatgaaac ggctccaggc tgctggcaat gcagtgaaga gggcctcaga
     8821 taacctggta aaggcggccc agaaagctgc agccttcgaa gaccaggaga atgagacggt
     8881 ggtggtgaag gagaaaatgg ttgggggcat tgcccagatt atcgcagcac aggaagagat
     8941 gcttcggaag gaacgagagc tggaagaggc tcggaaaaag ctcgcccaga tccggcagca
     9001 gcagtacaag ttcttgcctt cagagcttcg agacgagcac taaaaaagcc ctgtgtattt
     9061 aatgcagacc cagcccagag actgtgcctg ccactaccaa agccttctgg gctgctcagg
     9121 acccaagctg cccaacccca gcactccccc aaagtgcctg ccaaaccctg ggcctggccc
     9181 tgcccagtcc cactgcaagc cgtgtcctct ccctgactcc caagtgcctt tgcaccctag
     9241 ggcccctaag tgcctgcccc cctccagagt attaacgctc caagagtatt attaatgctg
     9301 ccgtacctca gtttgaacct gccagggccc cagctgctcc agcctgccag cagcttccag
     9361 ccagtcccca cggccacgtc agctcaactc atcccttttt gatactatat cccctaccca
     9421 gctacctatg gggcttgagg gttgtaaacc caaacaggtc agactcccgg aattcctgca
     9481 gcccggggga tccactagtt ctagagcggc cgcgactcta gagggcccgt ttaaacccgc
     9541 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     9601 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     9661 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     9721 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     9781 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
     9841 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     9901 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     9961 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
    10021 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
    10081 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
    10141 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
    10201 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
    10261 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
    10321 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
    10381 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
    10441 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
    10501 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
    10561 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca
    10621 ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat
    10681 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
    10741 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
    10801 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
    10861 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
    10921 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
    10981 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
    11041 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
    11101 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
    11161 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga
    11221 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
    11281 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
    11341 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
    11401 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
    11461 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
    11521 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
    11581 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
    11641 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
    11701 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
    11761 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
    11821 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
    11881 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
    11941 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
    12001 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
    12061 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
    12121 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
    12181 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
    12241 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
    12301 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
    12361 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
    12421 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
    12481 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
    12541 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
    12601 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
    12661 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
    12721 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
    12781 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
    12841 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
    12901 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
    12961 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
    13021 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
    13081 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
    13141 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
    13201 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
    13261 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
    13321 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
    13381 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
    13441 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
    13501 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
    13561 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
    13621 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
    13681 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    13741 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    13801 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    13861 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    13921 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat
    13981 cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg
    14041 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
    14101 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg
    14161 cttcgcgatg tacgggccag atatacgcgt t
//
