LOCUS       sequence-484329-         466 bp    DNA     linear   UNA 22-AUG-2025
DEFINITION  nested DIAL Reporter Plasmid with YB_TATA expressing mGreenLantern 
            in the presence of ZFa and editable by Cre and VCre recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 466)
  TITLE     Programmable promoter editing for precise control of transgene 
            expression
REFERENCE   2  (bases 1 to 466)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 22, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: (nested-DIAL-YB_TATA)-mGL-BGH_pKG2876
FEATURES             Location/Qualifiers
     source          1..466
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(123..156)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    212..236
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(356..389)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 agatctgaat tggagtgagg acgtgtagag tgaggacgtg cggagtgagg acgtgtagag
       61 tgaggacgtg cggagtgagg acgtgtagag tgaggacgtg ccctcctctg gatcaaatcc
      121 gaataacttc gtatagcata cattatacga agttatctcg agtcgctcgg tacgattcaa
      181 tttctgagaa ctgtcattct cggaaattga gccaactttg tacaaaaaag caggctccac
      241 catgggaacc aattcagtcg actggatccg gtacctccta cagctcctgg gcaacgtgct
      301 gtcaatttct gagaactgtc attctcggaa attgaattaa ttcgagcgaa cgcgtataac
      361 ttcgtatagc atacattata cgaagttatc ggatttgatc tgatcaagca actctagagg
      421 gtatataatg ggggccacta gtctactacc agagctcatc gctagc
//
