LOCUS       sequence-484335-         504 bp    DNA     linear   UNA 26-AUG-2025
DEFINITION  203-bp DIAL Reporter Plasmid with minCMV2 expressing mGreenLantern 
            in the presence of ZFa and editable by Cre recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 504)
  TITLE     Programmable promoter editing for precise control of transgene 
            expression
REFERENCE   2  (bases 1 to 504)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 26, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: (203-bp-DIAL-minCMV2)-mGL-BGH_pKG3680
FEATURES             Location/Qualifiers
     source          1..504
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(123..156)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     293..312
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(360..393)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        417..454
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     451..475
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 agatctgaat tggagtgagg acgtgtagag tgaggacgtg cggagtgagg acgtgtagag
       61 tgaggacgtg cggagtgagg acgtgtagag tgaggacgtg ccctcctctg gatcaaatcc
      121 gaataacttc gtatagcata cattatacga agttatctcg agtcgctcgg tacgatttaa
      181 attgaattct gcagtcgacg gtaccgcggg cccgggactt tgtacaaaaa agcaggctcc
      241 accatgggaa ccaattcagt cgactggatc cggtacctcc tacagctcct gggcaacgtg
      301 ctggttattg tgctgtctca tcattttggc aaagaattga ttaattcgag cgaacgcgta
      361 taacttcgta tagcatacat tatacgaagt tatcggattt gatctgatca agcaacgtag
      421 gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcctagt
      481 ctactaccag agctcatcgc tagc
//
