LOCUS       sequence-484490-       21235 bp    DNA     circular SYN 25-AUG-2025
DEFINITION  UBQ10 driven expression of STARBURST, a poly-cistronic fungal 
            bioluminescense pathway, in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 21235)
  AUTHORS   Dvir G, Xing Z, Beldman I, Rivera A, Wheeldon I, Cutler SR
  TITLE     Synthesis of large single-transcript pathways from oligonucleotide 
            pools: Design of STARBURST, an autobioluminescent reporter.
  JOURNAL   Proc Natl Acad Sci U S A. 2025 Aug 5;122(31):e2508109122. doi: 
            10.1073/pnas.2508109122. Epub 2025 Jul 29.
  PUBMED    40729380
REFERENCE   2  (bases 1 to 21235)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 25, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2025-08-5"; volume: "122(31):e2508109122. 
            doi"; pages: " 10.1073/pnas.2508109122. Epub 2025 Jul 29"
COMMENT     Sequence Label: pPlantPOP_STARBURST
FEATURES             Location/Qualifiers
     source          1..21235
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    188..212
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(258..510)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(550..1344)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(661..680)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1271..1290
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(1378..1557)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             complement(10390..10401)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(10564..10575)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        13171..13515
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     13459..13479
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             13589..14266
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(13769..13789)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     14181..14204
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     terminator      14290..14542
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(14577..14595)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    14784..14808
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             16109..16738
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             17167..18240
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      18306..18500
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(18736..18758)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    18844..18984
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     18899..18918
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(18999..19016)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(19170..19758)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(19250..19269)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(19846..20637)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cgcgagatca tccgtgtttc aaacccggca gcttagttgc cgttcttccg aatagcatcg
       61 gtaacatgag caaagtctgc cgccttacaa cggctctccc gctgacgccg tcccggactg
      121 atgggctgcc tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg agctgttggc
      181 tggctggtgg caggatatat tgtggtgtaa acataacgga tccggtctca ggagagcgat
      241 cagcttgcat gccggtcgat ctagtaacat agatgacacc gcgcgcgata atttatccta
      301 gtttgcgcgc tatattttgt tttctatcgc gtattaaatg tataattgcg ggactctaat
      361 cataaaaacc catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac
      421 gtaattcaac agaaattata tgataatcat ggcaagaccg gcaacaggat tcaatcttaa
      481 gaaactttat tgccaaatgt ttgaacgatc tgcttgactc tagctagagt ccgaacccca
      541 gagtcccgct cagaagaact cgtcaagaag gcgatagaag gctatgcgct gcgaatcggg
      601 agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc
      661 aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca
      721 gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcgtcgcc
      781 gtgggtcacg acgagatcct cgccgtcggg catccgcgcc ttgagcctgg cgaacagttc
      841 ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc
      901 catccgagta cgtgctcgct cgatgcggtg tttcgcttgg tggtcgaatg ggcaggtagc
      961 cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg
     1021 agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct
     1081 tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca
     1141 cgatagccgc gctgcctcgt cttggagttc attcagggca ccggacaggt cggtcttgac
     1201 aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat
     1261 tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc
     1321 gtgcaatcca tcttgttcaa tcatgcctcg atcgagttga gagtgaatat gagactctaa
     1381 ttggataccg aggggaattt atggaacgtc agtggagcat ttttgacaag aaatatttgc
     1441 tagctgatag tgaccttagg cgacttttga acgcgcaata atggtttctg acgtatgtgc
     1501 ttagctcatt aaactccaga aacccgcggc tcagtggctc cttcaacgtt gcggttctgt
     1561 cagttccaaa cgtaaaacgg cttgtcccgc gtcatcggcg ggggtcatac cgtgactccc
     1621 ttaattctca tgtatctccg tcaggagtct caatggtaac tttactcttt atttaaccat
     1681 acattttttt ttattttttt cactttgttc ttcatccact attgttcttt gttcatcttg
     1741 aacaaaagct ccctccttct ttgttcttca tccaccattg ttcttcatca atcatttcgc
     1801 tgtcaggagg tcgacgagtc agtaataaac ggcgtcaaag tggttgcagc cggcacacac
     1861 gagtcgtgtt tatcaactca aagcacaaat acttttcctc aacctaaaaa taaggcaatt
     1921 agccaaaaac aactttgcgt gtaaacaacg ctcaatacac gtgtcatttt attattagct
     1981 attgcttcac cgccttagct ttctcgtgac ctagtcgtcc tcgtcttttc ttcttcttct
     2041 tctataaaac aatacccaaa gagctcttct tcttcacaat tcagatttca atttctcaaa
     2101 atcttaaaaa ctttctctca attctctcta ccgtgatcaa ggtaaatttc tgtgttcctt
     2161 attctctcaa aatcttcgat tttgttttcg ttcgatccca atttcgtata tgttctttgg
     2221 tttagattct gttaatctta gatcgaagac gattttctgg gtttgatcgt tagatatcat
     2281 cttaattctc gattagggtt tcatagatat catccgattt gttcaaataa tttgagtttt
     2341 gtcgaataat tactcttcga tttgtgattt ctatctagat ctggtgttag tttctagttt
     2401 gtgcgatcga atttgtcgat taatctgagt ttttctgatt aacagaatgc ggtctctaat
     2461 gtctgcttca tcatcttatt ctgaattgga atcgccaaca tccctgttgg atgtgttcgt
     2521 ccatgctgca cgggatcctc atacagctag tcggagggta ttggaatgtg gctcagatac
     2581 gtggacgtac gcagctctgg atgcagtttc tgatggtata gctcgcgaat tggctccgtt
     2641 tggtctggca cctaaggtgg ctgtagttag cgaaaaccat cccttcgtct ttgccctgct
     2701 ctttgccgtg tggaaactgg gtggaacttt catcccaatc gacgcacacg taccgttcgc
     2761 aatgctcaca gggatggtga acatcgtgaa gcctacctgc ttgtacttgc cagcctctgc
     2821 aacatcgaac atatcgctgg tcaaggcctt cgacattcgg acagtggtct ttggccacaa
     2881 ggagaactcc atgcaggctc tcttcgacaa atacagcctg catgctgcac cattgcatgc
     2941 agcacccttg cattatgcgc caccatcagc tgatcacgct tgcctttacc tcttcacatc
     3001 ctccgcctca tctacgaaga acctcaaggc cgtgccattg acccatacac ttgtacttcg
     3061 gggatgtcaa tctaagctgg cttggtggcg cagggtacaa cctggcaaaa acttggatgc
     3121 gatcaggatt cttggttggg ctccttggtc ccatgtcctt gcctacatgc aggatatagg
     3181 gacagccaca ttgctgaacg cgggttgcta cgtgttcgca acaatcccat cgtcatatcc
     3241 tcagcttcct acgaccacgc ccgttgatct gaccactagt cttatcgatg ctcttgtaac
     3301 tcggcgggta gctgccttcg cctgtgttcc tttcgtcctt gcaaatctta aggcggcttg
     3361 tcagtcgaac catccggctc gctcacaact tcttgaggcc ttgcaaaaga ccatcatgct
     3421 ggagtgtgga ggggctgtct tggatgatgc tacagtcgat tgggctgaac gcaatggcat
     3481 tcgcatcttc accggcatcg ggatgacgga aactggtgga gctgtgtttg ttggactggc
     3541 aagcgagtct aggcgagggt ttttgcctga agggcttttg ggagatgcgt cattcagcat
     3601 ctcgtctgac accgacgcat tggatgaagg ggagcttgtc gtcaaatcca agctaatagc
     3661 gccgggatac gtcggctatg atgatggagc tcactcggtg gattgtgatg gctgggttac
     3721 gtttcggaca ggagataggt atcgccaaac tcaaccggac ggtcggttta cctggcttgg
     3781 aaggatcacg gatttcatcc agatggtttc tggggagtac cttgaccctc gacctctgga
     3841 agaatctctc agggcttcac cattgatagc gaacgcgtgt atagtaggcg acgccttcct
     3901 aagttcggct tcaaccagca ttctggccat catcgaactc gctactcctg acctggctca
     3961 tactgcttca attcgggctc aacttgcaag ggtcctagcc cctctaaatc gggatcttcc
     4021 tccaccattg cgcatagccg ctagcagtat tctggttctg gatggtatgc ggaagatccc
     4081 caagacgaag aagggggaca tcttccgcaa aaagctggag gacacgtttg gtgcggagtt
     4141 tgagcagatg ttgaggaccg agaaagtcgg tcttggagat cttgctgacg ttgatgccgg
     4201 cataactcgg atcatcggca atctgcttgg catcagcgac gatgagttgc tttcgacgat
     4261 gtcatttgcc gagctgggaa tgacctcgct cctggcagtg aagattgcct cagagctgaa
     4321 caagttcctt gatggtcgcg ctgtgttgcc caccaacatc tgctacatcc acttcgacgt
     4381 tccttcgttg acatccagtg tgagggaacg gctatcgagt gcgccaagtt ctatcacttc
     4441 tgctgctagt gaacctgcgg cttcttctcc cggaacacct agggccgatg aaatcgtgat
     4501 agtcggcaag gcttttcggc ttcctgacgg agttaatgac gacgccgctc tttgggatgt
     4561 gttgactggg gagtccgcta gcatcatcaa ggacattcct gcggatcgct gggatcatgc
     4621 gtctttctac cccaaggaca tccattttgg acgtgcaggt ttggtggatg tcgctcgctt
     4681 cgactatggg ttcttcggaa tgacggcaag tgaagcctac tcattgagtc ctaccatgcg
     4741 gctggcattg gaggtagcct atgaggctct cgaagatgcc aatattccat tccgcgccgt
     4801 taagggttcc aggatgggtg tgttcgttgc tgtgaaagac gatgggtttg agactctgct
     4861 ccacgctgga cagggctatg acgcttatac ccggttttat ggaacaggaa gggctccatc
     4921 tactgcaagc gggcggatca attacctcct tgaccttcat ggaccatcaa tcaccgtgga
     4981 cacagcttgc tcaggtggaa tcgtgtgcat cgatcaagct gtgacgtact tgcagtctgg
     5041 tgcagctgaa acagcgatcg tgtgctcgag taacacccat tgttggcctg gatcgttcat
     5101 gttcctgaca gctcagggta tggcctctcc taatggtcgg tgtgcatcgt ttacttctga
     5161 cgccgacgga tatgctccta gtgagggtgc tgttggattc gtcctaaaga caaggagtgc
     5221 tgcagtgcgg gatggtgatc ggatattggc tacgattagg gctaccgaga ttggtcacaa
     5281 cgggaggtca caaggacttg ctgctccgaa tgttagatca caagctgctg cacatcgggc
     5341 agtacttagg cgcgctcgtc ttgatccttc tgaaatcgac ttcatcgaag cgcatgggac
     5401 aggaacgaca ctgggggatc tttgtgaagt gcagggaatc aatgacagct tcgtgtcccc
     5461 caaaaaacgc gccaaccctc tggttgtatc tgcgtcgaag tccacaattg gacacaccga
     5521 accatcagca ggcttggtcg gaatcttgtc ggctctcatg agtttcgaga agcggattgt
     5581 gccacgcttg gcttacctca cagagtctaa cgtgaaccct gcacttgatg cctcagtcgt
     5641 tccactacac ttcccaacga agcacatcga acttcgcgcg gatgtgccat acaaggcagt
     5701 agtgatgtcc tatggatttg cagggacgct tgccgacatc gtccttgagt ctgaggttcc
     5761 tcaaccaact ccagcagttg ctcaggatac tgctgggcaa cagccaatgc ttttcgtgct
     5821 gagtgcaaag acaccacgtg cattggctgc gtacatcgag ctttacctcg ggtttcttcg
     5881 tcatgctgat cctggcttgt tcgcacggat ctgttacact gcatgcgttg cacgggaaca
     5941 ttacaagcat cgggttgcct gtgtggcaac agatctggtg gacttgattg cccagctgga
     6001 aactaggttg gtgcaaactg cttatgctgg aggtggaggt gctagggctg ctaggactgg
     6061 tcctcttgtg tttgctttta gtggacaggg cacacagttt ccagctatgg ctgcgccact
     6121 tgctcgcaga tatgcacgtt ttggggagat tgtaggtgga tgtgctagga tggcaaggga
     6181 actcagcggt ttcccagtag atggcatcct acttggcgat gacgttaccc ccgttaaaga
     6241 caattcggct gcagctgagg ttcactccga agtggaccag atctgcatct tcgtgtacca
     6301 gtacgctatg tgtcgctggc taggggagct tggtgttgag cctaaagctg cgataggaca
     6361 ctctttgggg gaaattacgg cggctgttat tgcaggggcg cttccatttg aagcggctct
     6421 tgatctggtt gtcactcgtg cgaggctcct taaaccatgt gcagaacaac cctcaggaat
     6481 ggcagcattg gcctgtacac ctgacgttgc ctccaaactc actcttggag cttcagtgtc
     6541 tgtgtcggtc tacaacgggc ctcagtccat ttgcctttct ggtgcttctg ctgaactgga
     6601 tgatgcagtg agggccgcta aagcgcgcaa tatcaaagcg acaaggcttc aagtcgacca
     6661 agggtttcac tcgccatgcg ttgatgctgc tgttccagga ctacaagctt ggtgtgcagc
     6721 acatcgtgct tcagctgctc ctcttaagat gccactctac tcgactgttc gcggtgatgt
     6781 tgttcctaaa ggagccgccc ttgatccaga acattgggtt gcccatgcaa ggaatcccgt
     6841 actcttcgct caaacggctc aggcgcttaa agagtctctt ccccatggga ttacactcga
     6901 tgtcggtcct caagccgttg cttggtcact cctattgctg aacggtctgt ctgcaactcg
     6961 gacagttgct gcaggagcta aaaagggtgc tgatcaagag agggcccttc ttggtgctct
     7021 tggagcgctt tttgagcagc acaaagtcac tcccgatttt ggccgcctgt atgctccttt
     7081 ggagaaaact cggatcccta cctacccttt tgagcgcgct aggtgctatc caacctttat
     7141 cccatcacgc tttgctcatg gtgcagccgc tgctgcaaaa actgatggag aagtcctttc
     7201 agcggaagag gagaatgtgg cacccgtggg acttctcacg aaagaagatc ttcgcgcagc
     7261 tttggtagct tgcttgaggg ccacactaga actaaggcct gacgaagaat tggacgaggc
     7321 tgaacccttg accgttcacg gtgtcgattc catagggttc gcaaagctta ggaagcacgt
     7381 ggaagatcgc tggggtttgg acattccggt cgtttattgg tctgatgcct tcaccgtggg
     7441 tgagatgttg ggtaacctgg ttggtcagta cgacgttgtt tcaacagctg cacggggaag
     7501 tggatcaaat gcggcggatg aagttgccac acgtcagttg cttaacttcg acctcctcaa
     7561 gctggctggt gacgtggaat caaatccggg acctatggtc caagatacgt cctctgcatc
     7621 caccagtccg atcttgactc ggtggtacat agatactcgc cctcttactg catctaccgc
     7681 tgctctccca ctattggaaa ccctccaacc tgctgaccag atctcagtcc agaagtacta
     7741 ccacctgaag gacaagcaca tgtccctcgc gtccaatctg ctcaagtacc tcttcgtaca
     7801 ccggaactgt aggataccct ggtcgtccat cgtaattagt cggactcccg atcctcatag
     7861 gcgtccgtgt tacattcccc caagtggttc acaagaggat tcgttcaagg acggctacac
     7921 ggggatcaac gtcgagttca acgtgtctca ccaagcatcg atggtcgcaa ttgcagggac
     7981 tgccttcaca cccaattcag gtggagactc caagctcaag ccggaggttg gcatagacat
     8041 aacctgcgta aatgaacggc aaggacggaa tggggaggaa cgctcgctag agtcacttcg
     8101 gcagtacata gacatcttct ctgaggtgtt ctcgacggcg gagatggcca atatacgccg
     8161 attggatggt gtctcatcct catctctgtc tgccgatcgg ctggtagact acggttaccg
     8221 gctgttctac acgtactggg cacttaagga agcctacatc aagatgacgg gagaggcttt
     8281 gctggcacca tggttgcgag agcttgagtt ctctaatgtc gttgcacctg ctgccgtagc
     8341 tgaaagtgga gacagtgctg gagattttgg cgaaccatac accggagtcc gaaccaccct
     8401 ttacaagaac ctggttgagg acgttcgcat cgaagttgca gctctgggtg gtgattacct
     8461 ttttgcgact gctgcacgag gtggtggaat aggagcaagt agtcgacctg gaggaggtcc
     8521 tgatggaagt ggtatacgga gtcaagatcc gtggaggcct ttcaagaagc tcgacatcga
     8581 acgcgacatt cagccttgcg ctaccggagt atgtaattgt ctgtctcgcg gaagtggaag
     8641 caatgcagct gacgaggtag ctacccggca actcctcaac tttgatctcc tgaagctagc
     8701 aggggatgtg gagagcaacc caggaccaat ggcgtccttt gaaaacagcc tgagcgtcct
     8761 gatagttgga gctggattgg gaggactagc agcagctatt gctttgcgac gacaagggca
     8821 cgtcgtcaag atctatgagt ccagctcctt caaagcggaa ctaggagcag gtttggcagt
     8881 cccaccaaat acactacgtt ccttgcagca gctcggctgc aacacggaga atctgaatgg
     8941 tgtcgacaat ttgtgcttca cggcgatggg ctacgatggc tcagtgggca tgatgaacaa
     9001 catgacggat tatcgtgagg cgtatggcac ctcctggatc atggtccacc gggttgacct
     9061 ccataatgaa ttgatgcgtg ttgctttgga tccaggggga cttgggccac cagcaactct
     9121 gcatcttaat caccgggtca ctttttgcga cgtcgacgca tgcacggtga cctttacgaa
     9181 cggtacaact cagtctgccg atcttatcgt tggggctgac gggatacgtt ccaccattcg
     9241 tcggtttgtc ttggaggagg atgtgaccat accagcaagc ggaattgtgg gctttcgatg
     9301 gctggttcaa gcagatgcgt tggatcctta cccagagctc gactggattg tgaagaagcc
     9361 tcctcttgga gctcgcttga ttagcacccc acaaaacccg caatctggag ttggacttgc
     9421 tgatcgtcgc accatcatca tctatgcatg tcgtgggggt acgatggtca acgtactcgc
     9481 agtccatgac gatgagaggg atcaaaatac cgctgactgg tcggttccgg ctagtaagga
     9541 tgacctcttc cgagtgttcc atgactacca tccccgtttt cggcgccttt tggaacttgc
     9601 tcaggacatc aacctctggc agatgcgagt agtgcctgtg ctgaagaagt gggtgaacaa
     9661 gcgagtgtgt ctgttgggag atgctgctca cgctatgcta cctactttgg gacagggctt
     9721 tggaatgggt cttgaggacg ctgtagctct tggaactctc cttccgaaag ggacgacagc
     9781 atctcagatc gaaacacggc tcgcggttta tgaacagcta cggaaggatc gagcagagtt
     9841 tgtagcggct gagagctacg aggagcagta tgttcctgaa aaacgcggtc tgtacctgcg
     9901 ttccaaagaa ctgcgcgatc gggttatggg ttacgacatc aaggtcgagt ccgagaaggt
     9961 gcttgaaacg ctccttcgga gctctaattc agctggatct ggctcaaacg ctgctgatga
    10021 agtagccact cgccagctgc tcaactttga cctcctaaaa ctggcagggg acgtagaatc
    10081 caatccaggt ccgatgaggt caactatctc gctgtcgagc ttgcttgaac ggctgagcaa
    10141 gttgtcatcg cgaagtatcg cgataacgtg tggtgtcgtc ctagcaagtg cgatagcatt
    10201 cccgatcatt cgacgggatt accagacctt cctggaagtc ggacctagct atgcaccgca
    10261 aaactttcgg ggatacatca cggtctgtgt cctctcgcta ttccgacagg aacagaaggg
    10321 gttggccatc tatgatcgcc ttccagagaa acgtcgatgg ttggctgatc tgccgtttcg
    10381 tgaagggcca cgtccttcaa tcactagtca catcatacaa cgccaacgga cgcagctggt
    10441 agaccaggag ttcgcaactc gagaactcat agacaaagtg attccgcggg tccaagcacg
    10501 gcatacggac aagactttcc tgtcaacctc caagtttgag ttccacgcca aggccatctt
    10561 cctcctccct tctatcccca ttaacgaccc gttgaacatc ccttctcacg acaccgttcg
    10621 tcgcacaaag cgcgagatag ctcacatgca tgattaccac gactgctctc tgcatctcgc
    10681 tctagcacct caggacggga aggaagttct caaaaagggt tggggacaac gtcatccact
    10741 tgctggacct ggagtacctg gtcctccaac tgaatggacg ttcctatatg ctcccaggaa
    10801 cgaagaggaa gcacgggttg tcgagatgat agtggaagcc tccatcgggt acatgacgaa
    10861 cgatcccgct ggcaagattg tcgaaaatgc aaagggaagc ggcagtaacg cagcagatga
    10921 ggttgcgact cggcagctac taaacttcga cctgctcaag ctagctggtg atgtggaaag
    10981 caatccggga cccatggcac ctatatcatc cacatggagt aggctcatcc gtttcgtagc
    11041 cgtggagact tccctcgttc atatcggtga accgatcgat gcgacaatgg acgttgggct
    11101 agctcgtcgt gaaggcaaga caatccaagc atacgagatc ataggctcgg gttcagctct
    11161 cgacctcagc gcacaagtta gcaagaatgt cctcactgta cgtgagctgc taatgccgct
    11221 aagccgggaa gagatcaaga ccgttcgatg tctaggcttg aactacccgg ttcacgcgac
    11281 tgaagctaac gttgcagtcc cgaagtttcc caacctgttc tacaagcctg tgacgagctt
    11341 gataggaccg ggaggactga tcacgatacc tagcgtagtt cagccaccta aagagcacca
    11401 gtctgactac gaggccgaac tggtcatcgt gataggcaaa gcagctaaga acgtgtccga
    11461 ggatgaagcc ctggactacg ttctcggtta cactgctgcg aatgacatca gtttccgcaa
    11521 gcaccaactc gccgtcagtc agtggagctt ctccaagggg tttgacggaa caaatccttt
    11581 gggtccttgt ctggtttcgg caagctctat cccggacccg caagacatac ctatccaatg
    11641 caagttgaac ggtggggttg tccagaatgg caacacccgg gaccagatct tcaacgtgaa
    11701 gaaaacgatc tcgttcctca gccaagggac tacgctggag cccggctcta ttatccttac
    11761 cggtactcct gatggtgtcg gattcgttcg caatcccccg ctgtacctga aagatggaga
    11821 tgaagtgatg acatggatag gcagcgggat agggacgctc gccaacactg ttcgagaaga
    11881 acaaacatgt tttgcatcag gaggtcatga atgagcttag agaccgctta gctttcgttc
    11941 gtatcatcgg tttcgacaac gttcgtcaag ttcaatgcat cagtttcatt gcgcacacac
    12001 cagaatccta ctgagtttga gtattatggc attgggaaaa ctgtttttct tgtaccattt
    12061 gttgtgcttg taatttactg tgttttttat tcggttttcg ctatcgaact gtgaaatgga
    12121 aatggatgga gaagagttaa tgaatgatat ggtccttttg ttcattctca aattaatatt
    12181 atttgttttt tctcttattt gttgtgtgtt gaatttgaaa ttataagaga tatgcaaaca
    12241 ttttgttttg agtaaaaatg tgtcaaatcg tggcctctaa tgaccgaagt taatatgagg
    12301 agtaaaacac ttgtagttgt accattatgc ttattcacta ggcaacaaat atattttcag
    12361 acctagaaaa gctgcaaatg ttactgaata caagtatgtc ctcttgtgtt ttagacattt
    12421 atgaactttc ctttatgtaa ttttccagaa tccttgtcag attctaatca ttgctttata
    12481 attatagtta tactcatgga tttgtagttg agtatgaaaa tattttttaa tgcattttat
    12541 gacttgccgc tgtcaggaga ctagagccaa gctgatctcc tttgccccgg agatcaccat
    12601 ggacgacttt ctctatctct acgatctagg aagaaagttc gacggagaag gtgacgatac
    12661 catgttcacc accgataatg agaagattag cctcttcaat ttcagaaaga atgctgaccc
    12721 acagatggtt agagaggcct acgcggcagg tctgatcaag acgatctacc cgagtaataa
    12781 tctccaggag atcaaatacc ttcccaagaa ggttaaagat gcagtcaaaa gattcaggac
    12841 taactgcatc aagaacacag agaaagatat atttctcaag atcagaagta ctattccagt
    12901 atggacgatt caaggcttgc ttcataaacc aaggcaagta atagagattg gagtctctaa
    12961 gaaagtagtt cctactgaat caaaggccat ggagtcaaaa attcagatcg aggatctaac
    13021 agaactcgcc gtgaagactg gcgaacagtt catacagagt cttttacgac tcaatgacaa
    13081 gaagaaaatc ttcgtcaaca tggtggagca cgacactctc gtctactcca agaatatcaa
    13141 agatacagtc tcagaagacc aaagggctat tgagactttt caacaaaggg taatatcggg
    13201 aaacctcctc ggattccatt gcccagctat ctgtcacttc atcaaaagga cagtagaaaa
    13261 ggaaggtggc acctacaaat gccatcattg cgataaagga aaggctatcg ttcaagatgc
    13321 ccctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga
    13381 agacgttcca accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag
    13441 ggatgacgca caatcccact atccttcgca agacccttcc tctatataag gaagttcatt
    13501 tcatttggag aggactccgg tatttttaca acaattacca caacaaaaca aacaacaaac
    13561 aacattacaa tttactattc tagtcgaaat ggcctcctcc gagaacgtca tcaccgagtt
    13621 catgcgcttc aaggtgcgca tggagggcac cgtgaacggc cacgagttcg agatcgaggg
    13681 cgagggcgag ggccgcccct acgagggcca caacaccgtg aagctgaagg tgaccaaggg
    13741 cggccccctg cccttcgcct gggacatcct gtccccccag ttccagtacg gctccaaggt
    13801 gtacgtgaag caccccgccg acatccccga ctacaagaag ctgtccttcc ccgagggctt
    13861 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gcgaccgtga cccaggactc
    13921 ctccctgcag gacggctgct tcatctacaa ggtgaagttc atcggcgtga acttcccctc
    13981 cgacggcccc gtgatgcaga agaagacgat gggctgggag gcctccaccg agcgcctgta
    14041 cccccgcgac ggcgtgctga agggcgagac acacaaggcc ctgaagctga aggacggcgg
    14101 ccactacctg gtggagttca agtccatcta catggccaag aagcccgtgc agctgcccgg
    14161 ctactactac gtggacgcca agctggacat cacctcccac aacgaggact acaccatcgt
    14221 ggagcagtac gagcgcaccg agggccgcca ccacctgttc ctgtaggctt ggaatggatc
    14281 ttcgatcccg atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc
    14341 ggtcttgcga cgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac
    14401 atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac
    14461 atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg
    14521 gtgtcatcta tgttactaga tcgggaattg ccaagctaat tcttgaagac gaaagggcct
    14581 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
    14641 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
    14701 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat gggaccgact
    14761 cgcgctgtca tgagaccgga tcctgacagg atatattggc gggtaaacct aagagaaaag
    14821 agcgtttatt agaataatcg gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca
    14881 tttgtatgtg catgccaacc acagggttcc cctcgggatc aaagtacttt gatccaaccc
    14941 ctccgctgct atagtgcagt cggcttctga cgttcagtgc agccgtcatc tgaaaacgac
    15001 atgtcgcaca agtcctaagt tacgcgacag gctgccgccc tgcccttttc ctggcgtttt
    15061 cttgtcgcgt gttttagtcg cataaagtag aatacttgcg actagaaccg gagacattac
    15121 gccatgaaca agagcgccgc cgctggcctg ctgggctatg cccgcgtcag caccgacgac
    15181 caggacttga ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa gctgttttcc
    15241 gagaagatca ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta
    15301 cgccctggcg acgttgtgac agtgaccagg ctagaccgcc tggcccgcag cacccgcgac
    15361 ctactggaca ttgccgagcg catccaggag gccggcgcgg gcctgcgtag cctggcagag
    15421 ccgtgggccg acaccaccac gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt
    15481 gccgagttcg agcgttccct aatcatcgac cgcacccgga gcgggcgcga ggccgccaag
    15541 gcccgaggcg tgaagtttgg cccccgccct accctcaccc cggcacagat cgcgcacgcc
    15601 cgcgagctga tcgaccagga aggccgcacc gtgaaagagg cggctgcact gcttggcgtg
    15661 catcgctcga ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc
    15721 aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc
    15781 gagaatgaac gccaagagga acaagcatga aaccgcacca ggacggccag gacgaaccgt
    15841 ttttcattac cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc
    15901 ccgcgcacct ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct gatgccaagc
    15961 tggcggcctg gccggccagc ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt
    16021 gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag
    16081 taaataaaca aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg
    16141 cgggtcaggc aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc
    16201 cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg
    16261 ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa
    16321 ggccatcggc cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc
    16381 tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga
    16441 catatgggcc accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg
    16501 aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga
    16561 ggttgccgag gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg
    16621 cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg
    16681 cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt
    16741 taatgaggta aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc
    16801 gcacgcagca gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg
    16861 gtcaactttc agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa
    16921 ggcaagacca ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc
    16981 aaatgaataa atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga
    17041 acaaccaggc accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg
    17101 cgtaagcggc tgggttgcct gccggccctg caatggcact ggaaccccca agcccgagga
    17161 atcggcgtga gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc gctgggtgat
    17221 gacctggtgg agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa
    17281 gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa
    17341 ccgccggcag ccggtgcgcc gtcgattagg aagccgccca agggcgacga gcaaccagat
    17401 tttttcgttc cgatgctcta tgacgtgggc acccgcgata gtcgcagcat catggacgtg
    17461 gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg ctacgagctt
    17521 ccagacgggc acgtagaggt ttccgcaggg ccggccggca tggccagtgt gtgggattac
    17581 gacctggtac tgatggcggt ttcccatcta accgaatcca tgaaccgata ccgggaaggg
    17641 aagggagaca agcccggccg cgtgttccgt ccacacgttg cggacgtact caagttctgc
    17701 cggcgagccg atggcggaaa gcagaaagac gacctggtag aaacctgcat tcggttaaac
    17761 accacgcacg ttgccatgca gcgtacgaag aaggccaaga acggccgcct ggtgacggta
    17821 tccgagggtg aagccttgat tagccgctac aagatcgtaa agagcgaaac cgggcggccg
    17881 gagtacatcg agatcgagct agctgattgg atgtaccgcg agatcacaga aggcaagaac
    17941 ccggacgtgc tgacggttca ccccgattac tttttgatcg atcccggcat cggccgtttt
    18001 ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag ccagatggtt gttcaagacg
    18061 atctacgaac gcagtggcag cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag
    18121 ctgatcgggt caaatgacct gccggagtac gatttgaagg aggaggcggg gcaggctggc
    18181 ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc cggttcctaa
    18241 tgtacggagc agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg aaaaggactc
    18301 tttcctgtgg atagcacgta cattgggaac ccaaagccgt acattgggaa ccggaacccg
    18361 tacattggga acccaaagcc gtacattggg aaccggtcac acatgtaagt gactgatata
    18421 aaagagaaaa aaggcgattt ttccgcctaa aactctttaa aacttattaa aactcttaaa
    18481 acccgcctgg cctgtgcata actgtctggc cagcgcacag ccgaagagct gcaaaaagcg
    18541 cctacccttc ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc
    18601 gctggccgct caaaaatggc tggcctacgg ccaggcaatc taccagggcg cggacaagcc
    18661 gcgccgtcgc cactcgaccg ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg
    18721 tgatgacggt gaaaacctct gacacatgca gctcccggtg acggtcacag cttgtctgta
    18781 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
    18841 gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg
    18901 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
    18961 gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc
    19021 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
    19081 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
    19141 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
    19201 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
    19261 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
    19321 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
    19381 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
    19441 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
    19501 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
    19561 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
    19621 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
    19681 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
    19741 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
    19801 aacgaaaact cacgttaagg gattttggtc atgcattcta ggtgattatt tgccgactac
    19861 cttggtgatc tcgcctttca cgtagtggac aaattcttcc aactgatctg cgcgcgaggc
    19921 caagcgatct tcttcttgtc caagataagc ctgtctagct tcaagtatga cgggctgata
    19981 ctgggccggc aggcgctcca ttgcccagtc ggcagcgaca tccttcggcg cgattttgcc
    20041 ggttactgcg ctgtaccaaa tgcgggacaa cgtaagcact acatttcgct catcaccagc
    20101 ccagtcgggc ggcgagttcc atagcgttaa ggtttcattt agcgcctcaa atagatcctg
    20161 ttcaggaacc ggatcaaaga gttcctccgc cgctggacct accaaggcaa cgctatgttc
    20221 tcttgctttt gtcagcaaga tagccagatc aatgtcgatc gtggctggct cgaagatacc
    20281 tgcaagaatg tcattgcgct gccattctcc aaattgcagt tcgcgcttag ctggataacg
    20341 ccacggaatg atgtcgtcgt gcacaacaat ggtgacttct acagcgcgga gaatctcgct
    20401 ctctccaggg gaagccgaag tttccaaaag gtcgttgatc aaagctcgcc gcgttgtttc
    20461 atcaagcctt acggtcaccg taaccagcaa atcaatatca ctgtgtggct tcaggccgcc
    20521 atccactgcg gagccgtaca aatgtacggc cagcaacgtc ggttcgagat ggcgctcgat
    20581 gacgccaact acctctgata gttgagtcga tacttcggcg atcaccgctt ccctcataat
    20641 gtttaacttt gttttagggc gactgccctg ctgcgtaaca tcgttgctgc tccataacat
    20701 caaacatcga cccacggcgt aacgcgcttg ctgcttggat gcccgaggca tagactgtac
    20761 cccaaaaaaa cagtcataac aagccatgaa aaccgccact gcgccgttac caccgctgcg
    20821 ttcggtcaag gttctggacc agttgcgtga gcgcatacgc tacttgcatt acagcttacg
    20881 aaccgaacag gcttatgtcc actgggttcg tgccttcatc cgtttccacg gtgtgcgtca
    20941 cccggcaacc ttgggtagca gcgaagtcga ggcatttctg tcctggctgg aacagaactt
    21001 attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa
    21061 gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt
    21121 ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc
    21181 gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctc
//
