LOCUS       sequence-484493-        9130 bp    DNA     circular SYN 25-AUG-2025
DEFINITION  Stable genomic integration of Opto-IRE1 
            (HsIRE1dLD-mCherry-CRY2clust) using the Flp-in system..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9130)
  AUTHORS   Smith JW, Wilburn DB, Belyy V
  TITLE     Optogenetic Clustering of Human IRE1 Reveals Differential Regulation
            of Transcription and mRNA Splice Isoform Abundance by the UPR.
  JOURNAL   bioRxiv [Preprint]. 2025 Jul 21:2025.07.16.665212. doi: 
            10.1101/2025.07.16.665212.
  PUBMED    40777275
REFERENCE   2  (bases 1 to 9130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 25, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2025 Jul 21:2025.07.16.665212. doi: 
            10.1101/2025.07.16.665212."
COMMENT     Sequence Label: pVBL0001
FEATURES             Location/Qualifiers
     source          1..9130
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     996..1015
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        996..1014
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1044..1068
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3498..4991
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     protein_bind    complement(5023..5047)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5082..5099)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5088..5312
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5445..5464)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5596..5643
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5651..6673
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5651..6673
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6803..6924
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6840..6859)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6894..6913
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6973..6989)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6973..6989)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6986..7008)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6997..7013
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7021..7027,7028..7045,7046..7051))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7066..7087
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7204..7221)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7375..7963)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7455..7474)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8134..8925,8926..8994))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8757..8776
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8995..9099)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttgcttatc gaaattaata cgactcacta tagggagacc
     1021 caagctggct agttaagcta tcaacaagtt tgtacaaaaa agcaggcttc accatgccgg
     1081 cccggcggct gctgctgctg ctgacgctgc tgctgcccgg cctcgggatt tttggaagta
     1141 tgcttaagga catggctacc atcatcctga gcaccttcct gctgattggc tgggtggcct
     1201 tcatcatcac ctatcccctg agcatgcatc agcagcagca gctccagcac cagcagttcc
     1261 agaaggaact ggagaagatc cagctcctgc agcagcagca gcagcagctg cccttccacc
     1321 cacctggaga cacggctcag gacggcgagc tcctggacac gtctggcccg tactcagaga
     1381 gctcgggcac cagcagcccc agcacgtccc ccagggcctc caaccactcg ctctgctccg
     1441 gcagctctgc ctccaaggct ggcagcagcc cctccctgga acaagacgat ggagatgagg
     1501 aaaccagcgt ggtgatagtt gggaaaattt ccttctgtcc caaggatgtc ctgggccatg
     1561 gagctgaggg cacaattgtg taccggggca tgtttgacaa ccgcgacgtg gccgtgaaga
     1621 ggatcctccc cgagtgtttt agcttcgcag accgtgaggt ccagctgttg cgagaatcgg
     1681 atgagcaccc gaacgtgatc cgctacttct gcacggagaa ggaccggcaa ttccagtaca
     1741 ttgccatcga gctgtgtgca gccaccctgc aagagtatgt ggagcagaag gactttgcgc
     1801 atctcggcct ggagcccatc accttgctgc agcagaccac ctcgggcctg gcccacctcc
     1861 actccctcaa catcgttcac agagacctaa agccacacaa catcctcata tccatgccca
     1921 atgcacacgg caagatcaag gccatgatct ccgactttgg cctctgcaag aagctggcag
     1981 tgggcagaca cagtttcagc cgccgatctg gggtgcctgg cacagaaggc tggatcgctc
     2041 cagagatgct gagcgaagac tgtaaggaga accctaccta cacggtggac atcttttctg
     2101 caggctgcgt cttttactac gtaatctctg agggcagcca cccttttggc aagtccctgc
     2161 agcggcaggc caacatcctc ctgggtgcct gcagccttga ctgcttgcac ccagagaagc
     2221 acgaagacgt cattgcacgt gaattgatag agaagatgat tgcgatggat cctcagaaac
     2281 gcccctcagc gaagcacgtg ctcaaacacc cgttcttctg gagcctagag aagcagctcc
     2341 agttcttcca ggacgtgagc gacagaatag aaaaggaatc cctggatggc ccgatcgtga
     2401 agcagttaga gagaggcggg agagccgtgg tgaagatgga ctggcgggag aacatcactg
     2461 tccccctcca gacagacctg cgtaaattca ggacctataa aggtggttct gtcagagatc
     2521 tcctccgagc catgagaaat aagaagcacc actaccggga gctgcctgca gaggtgcggg
     2581 agacgctggg gtccctcccc gacgacttcg tgtgctactt cacgtctcgc ttcccccacc
     2641 tcctcgcaca cacctaccgg gccatggagc tgtgcagcca cgagagactc ttccagccct
     2701 actacttcca cgagccccca gagccccagc ccccagtgac tccagacgcc ctcggaggat
     2761 ctggaggcgg cgggtccggc ggaatggtgt ctaaaggcga ggaggataac atggctataa
     2821 tcaaggaatt tatgaggttc aaggtgcata tggagggatc tgtgaacggt cacgagttcg
     2881 aaatcgaggg cgaaggcgag gggcgaccct atgaagggac acagacggcc aaactgaagg
     2941 tgaccaaggg tggccccctg cctttcgcct gggacatcct gtcaccccag tttatgtacg
     3001 gctctaaggc ttacgttaag caccctgccg atattccgga ctacttgaaa ctgagcttcc
     3061 ctgaagggtt caagtgggaa cgggtaatga attttgagga tggcggtgta gtcacagtta
     3121 cccaggacag ctctcttcag gacggagaat ttatctataa ggttaaactt cggggcacta
     3181 acttcccatc agacggcccc gtgatgcaga agaaaactat gggctgggaa gccagctcag
     3241 aacgcatgta tcccgaggac ggagcactga agggagaaat caagcagcga ctcaagctga
     3301 aggatggggg acattatgat gctgaggtca aaaccaccta taaggccaag aaacccgttc
     3361 agcttcctgg ggcatacaac gtgaatatca aactggatat tacatcccat aacgaagact
     3421 ataccatcgt ggaacagtac gagcgggccg agggcagaca tagcacaggg ggcatggatg
     3481 aattgtacaa ggcggccatg aagatggaca aaaagactat agtttggttt agaagagacc
     3541 taaggattga ggataatcct gcattagcag cagctgctca cgaaggatct gtttttcctg
     3601 tcttcatttg gtgtcctgaa gaagaaggac agttttatcc tggaagagct tcaagatggt
     3661 ggatgaaaca atcacttgct cacttatctc aatccttgaa ggctcttgga tctgacctca
     3721 ctttaatcaa aacccacaac acgatttcag cgatcttgga ttgtatccgc gttaccggtg
     3781 ctacaaaagt cgtctttaac cacctctatg atcctgtttc gttagttcgg gaccataccg
     3841 taaaggagaa gctggtggaa cgtgggatct ctgtgcaaag ctacaatgga gatctattgt
     3901 atgaaccgtg ggagatatac tgcgaaaagg gcaaaccttt tacgagtttc aattcttact
     3961 ggaagaaatg cttagatatg tcgattgaat ccgttatgct tcctcctcct tggcggttga
     4021 tgccaataac tgcagcggct gaagcgattt gggcgtgttc gattgaagaa ctagggctgg
     4081 agaatgaggc cgagaaaccg agcaatgcgt tgttaactag agcttggtct ccaggatgga
     4141 gcaatgctga taagttacta aatgagttca tcgagaagca gttgatagat tatgcaaaga
     4201 acagcaagaa agttgttggg aattctactt cactactttc tccgtatctc catttcgggg
     4261 aaataagcgt cagacacgtt ttccagtgtg cccggatgaa acaaattata tgggcaagag
     4321 ataagaacag tgaaggagaa gaaagtgcag atctttttct taggggaatc ggtttaagag
     4381 agtattctcg gtatatatgt ttcaacttcc cgtttactca cgagcaatcg ttgttgagtc
     4441 atcttcggtt tttcccttgg gatgctgatg ttgataagtt caaggcctgg agacaaggca
     4501 ggaccggtta tccgttggtg gatgccggaa tgagagagct ttgggctacc ggatggatgc
     4561 ataacagaat aagagtgatt gtttcaagct ttgctgtgaa gtttcttctc cttccatgga
     4621 aatggggaat gaagtatttc tgggatacac ttttggatgc tgatttggaa tgtgacatcc
     4681 ttggctggca gtatatctct gggagtatcc ccgatggcca cgagcttgat cgcttggaca
     4741 atcccgcgtt acaaggcgcc aaatatgacc cagaaggtga gtacataagg caatggcttc
     4801 ccgagcttgc gagattgcca actgaatgga tccatcatcc atgggacgct cctttaaccg
     4861 tactcaaagc ttctggtgtg gaactcggaa caaactatgc gaaacccatt gtagacatcg
     4921 acacagctcg tgagctacta gctaaagcta tttcaagaac ccgtgaagca cagatcatga
     4981 tcggagcagc agcccgggat ccaccggatc tggataactg acacccagct ttcttgtaca
     5041 aagtggttga tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag
     5101 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     5161 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     5221 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     5281 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     5341 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     5401 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     5461 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     5521 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     5581 tggttcacgt acctagaagt tcctattccg aagttcctat tctctagaaa gtataggaac
     5641 ttccttggcc aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa
     5701 gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag
     5761 cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta
     5821 caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct
     5881 tgacattggg gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt
     5941 cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc
     6001 catggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc
     6061 gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca
     6121 tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct
     6181 cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga
     6241 tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag
     6301 cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg
     6361 gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg
     6421 atcgccgcgg ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt
     6481 ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg
     6541 atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac
     6601 cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag
     6661 ggcaaaggaa tagcacgtac tacgagattt cgattccacc gccgccttct atgaaaggtt
     6721 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     6781 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     6841 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     6901 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     6961 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     7021 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     7081 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     7141 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     7201 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     7261 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     7321 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     7381 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     7441 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     7501 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     7561 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     7621 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     7681 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     7741 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     7801 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     7861 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     7921 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     7981 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     8041 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     8101 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     8161 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     8221 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     8281 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     8341 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     8401 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     8461 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     8521 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     8581 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     8641 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     8701 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     8761 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     8821 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     8881 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     8941 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     9001 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     9061 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     9121 acctgacgtc
//
