LOCUS       sequence-484527-        9895 bp    DNA     circular SYN 25-AUG-2025
DEFINITION  To test the cleavage of ACE2 by a non-autocleavable form of TMPRSS2 
            (Less active form).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9895)
  TITLE     Zaid Abassi Lab Plasmids 2025
REFERENCE   2  (bases 1 to 9895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 25, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: TMPRSS2-Mutant-R255A
FEATURES             Location/Qualifiers
     source          1..9895
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(719..743)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             751..792
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    843..1431
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(896..916)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1314..1325)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1503..1736
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1814..1935
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1851..1870)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1905..1924
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1975..2110
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2130..2149)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2131..2149)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2159..2176)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2159..2175)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2168..2190)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2317..2772
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2404..2423)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2614..2635
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2798..2902
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2903..2971,2972..3763)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3121..3140)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3934..4522
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4423..4442
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4676..4693
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4810..4831
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4846..4851,4852..4869,4870..4876)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4884..4900
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4889..4911
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4908..4924
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4908..4924
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4943..4963
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4945..4963
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4991..5217
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5218..5398
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5445..5570
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6063..6296
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6481..6525
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        6756..6966
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        6778..6974
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6825..6960
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7006..7608
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(7006..7025)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7502..7522
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7651..7768
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7906..9084
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          8137..9075
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     9032..9052
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    9104..9128
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gctattgtgg gcggcgagag cgcgctcccg ggggcctggc cctggcaggt cagcctgcac
       61 gtccagaacg tccacgtgtg cggaggctcc atcatcaccc ccgagtggat cgtgacagcc
      121 gcccactgcg tggaaaaacc tcttaacaat ccatggcatt ggacggcatt tgcggggatt
      181 ttgagacaat ctttcatgtt ctatggagcc ggataccaag tagaaaaagt gatttctcat
      241 ccaaattatg actccaagac caagaacaat gacattgcgc tgatgaagct gcagaagcct
      301 ctgactttca acgacctagt gaaaccagtg tgtctgccca acccaggcat gatgctgcag
      361 ccagaacagc tctgctggat ttccgggtgg ggggccaccg aggagaaagg gaagacctca
      421 gaagtgctga acgctgccaa ggtgcttctc attgagacac agagatgcaa cagcagatat
      481 gtctatgaca acctgatcac accagccatg atctgtgccg gcttcctgca ggggaacgtc
      541 gattcttgcc agggtgacag tggagggcct ctggtcactt cgaagaacaa tatctggtgg
      601 ctgatagggg atacaagctg gggttctggc tgtgccaaag cttacagacc aggagtgtac
      661 gggaatgtga tggtattcac ggactggatt tatcgacaaa tgagggcaga cggctaaaac
      721 ccagctttct tgtacaaagt ggttgatatc ggtaagccta tccctaaccc tctcctcggt
      781 ctcgattcta cgtagtaatg aactagtacc ggttaagtcg acaatcaacg cgttaagtcg
      841 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
      901 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
      961 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     1021 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     1081 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     1141 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     1201 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     1261 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     1321 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     1381 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cgtcgacttt
     1441 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     1501 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     1561 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     1621 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     1681 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtac
     1741 gtatagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     1801 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     1861 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     1921 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg
     1981 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     2041 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     2101 tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac
     2161 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     2221 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     2281 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa
     2341 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     2401 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     2461 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     2521 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     2581 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     2641 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     2701 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     2761 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     2821 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     2881 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     2941 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     3001 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     3061 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     3121 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     3181 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     3241 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     3301 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     3361 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     3421 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     3481 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     3541 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     3601 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     3661 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     3721 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     3781 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     3841 gatccttttt gataatctca tgaccaaaat cccttaacgt gatttttcgt tccactgagc
     3901 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     3961 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     4021 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     4081 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     4141 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     4201 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     4261 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     4321 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     4381 cggcagggtc gtaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     4441 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     4501 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     4561 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     4621 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     4681 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     4741 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     4801 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
     4861 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
     4921 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct
     4981 gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt
     5041 tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg
     5101 tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc
     5161 actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg
     5221 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     5281 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     5341 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     5401 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     5461 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     5521 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     5581 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     5641 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     5701 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     5761 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     5821 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     5881 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     5941 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     6001 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     6061 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     6121 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     6181 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     6241 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     6301 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     6361 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     6421 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     6481 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     6541 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     6601 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     6661 ttatcgtttc agacccacct cccaaccccg aggggaccca tgcattgcat ctcaattagt
     6721 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     6781 gtctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     6841 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     6901 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     6961 taggcttttg caaaaagctt tctagaggta ccaccggagc ttaccatgac cgagtacaag
     7021 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     7081 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     7141 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     7201 gtcgcggacg acggcgccgc cgtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     7261 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     7321 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     7381 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     7441 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     7501 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     7561 gaaggaccgc gcacctggtg catgacccgc aagcccggtg ccagttaacg aattctagat
     7621 cttgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
     7681 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     7741 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact
     7801 ttggcgccgg ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat
     7861 ctttgcagct aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt
     7921 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     7981 tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     8041 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     8101 gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc
     8161 gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg
     8221 cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct
     8281 tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc
     8341 cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta
     8401 gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta
     8461 aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg
     8521 gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga
     8581 atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg
     8641 tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa
     8701 agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga
     8761 gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct
     8821 tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt
     8881 tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact
     8941 gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt
     9001 gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt
     9061 tttcttccat ttcaggtgtc gtgaggctag catcgattga tcaacaagtt tgtacaaaaa
     9121 agcaggctac aaaatggctt tgaactcagg gtcaccacca gctattggac cttactatga
     9181 aaaccatgga taccaaccgg aaaaccccta tcccgcacag cccactgtgg tccccactgt
     9241 ctacgaggtg catccggctc agtactaccc gtcccccgtg ccccagtacg ccccgagggt
     9301 cctgacgcag gcttccaacc ccgtcgtctg cacgcagccc aaatccccat ccgggacagt
     9361 gtgcacctca aagactaaga aagcactgtg catcaccttg accctgggga ccttcctcgt
     9421 gggagctgcg ctggccgctg gcctactctg gaagttcatg ggcagcaagt gctccaactc
     9481 tgggatagag tgcgactcct caggtacctg catcaacccc tctaactggt gtgatggcgt
     9541 gtcacactgc cccggcgggg aggacgagaa tcggtgtgtt cgcctctacg gaccaaactt
     9601 catccttcag gtgtactcat ctcagaggaa gtcctggcac cctgtgtgcc aagacgactg
     9661 gaacgagaac tacgggcggg cggcctgcag ggacatgggc tataagaata atttttactc
     9721 tagccaagga atagtggatg acagcggatc caccagcttt atgaaactga acacaagtgc
     9781 cggcaatgtc gatatctata aaaaactgta ccacagtgat gcctgttctt caaaagcagt
     9841 ggtttcttta cgctgtatag cctgcggggt caacttgaac tcaagccgcc agagc
//
