LOCUS       sequence-484549-        2958 bp    DNA     circular SYN 25-AUG-2025
DEFINITION  Cloning gene libraries from oligonucleotide pools.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Dvir G, Xing Z, Beldman I, Rivera A, Wheeldon I, Cutler SR
  TITLE     Synthesis of large single-transcript pathways from oligonucleotide 
            pools: Design of STARBURST, an autobioluminescent reporter.
  JOURNAL   Proc Natl Acad Sci U S A. 2025 Aug 5;122(31):e2508109122. doi: 
            10.1073/pnas.2508109122. Epub 2025 Jul 29.
  PUBMED    40729380
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 25, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2025-08-5"; volume: "122(31):e2508109122. 
            doi"; pages: " 10.1073/pnas.2508109122. Epub 2025 Jul 29"
COMMENT     Sequence Label: pPOP-BsmBI
FEATURES             Location/Qualifiers
     source          1..2958
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     42..60
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(64..107)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    110..131
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        151..185
                     /label=BBa_J23106
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.47"
     RBS             194..205
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      885..956
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      972..999
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      1041..1098
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1122..1139)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1293..1881)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1373..1392)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2064..2158)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2179..2838)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2753..2772
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
       61 agaatttcag ataaaaaaaa tccttagctt tcgctaagga tgatttctgg aattcgcggc
      121 cgcttctaga gcgatgagta atgagagacg tttacggcta gctcagtcct aggtatagtg
      181 ctagctacta gagaaagagg agaaatacta aatgagtgtg atcgctaaac aaatgaccta
      241 caaggtttat atgtcaggca cggtcaatgg acactacttt gaggtcgaag gcgatggaaa
      301 aggtaagccc tacgaggggg agcagacggt aaagctcact gtcaccaagg gcggacctct
      361 gccatttgct tgggatattt tatcaccaca gtgtcagtac ggaagcatac cattcaccaa
      421 gtaccctgag gacatccctg actatgtaaa gcagtcattc ccggagggct atacatggga
      481 gaggatcatg aactttgaag atggtgcagt gtgtactgtc agcaatgatt ccagcatcca
      541 aggcaactgt ttcatctacc atgtcaagtt ctctggtttg aactttcctc ccaatggacc
      601 tgtcatgcag aagaaaacac agggctggga acccaacact gagcgtctgt ttgcacgaga
      661 tggaatgctg ctaggaaaca actttatggc tctgaagtta gaaggaggcg gtcactattt
      721 gtgtgaattt aaaactactt acaaggcaaa gaagcctgtg aagatgccag ggtatcacta
      781 tgttgaccgc aaactggatg taaccaatca caacaaggat tacacttcgg ttgagcagtg
      841 tgaaatttcc attgcacgca aacctgtggt cgcctaataa gcttcaaata aaacgaaagg
      901 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
      961 agagtcacac tggctcacct tcgggtgggc ctttctgcgc gtctctgctt actcatcgcg
     1021 actagtagcg gccgctgcag tccggcaaaa aagggcaagg tgtcaccacc ctgccctttt
     1081 tctttaaaac cgaaaagatt acttcgcgtt atgcaggctt cctcgctcac tgactcgctg
     1141 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     1201 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     1261 aggaaccgta aaaaggccgc gttgctggcg tttttccaya ggctccgccc ccctgacgag
     1321 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     1381 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     1441 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     1501 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     1561 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     1621 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     1681 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     1741 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     1801 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     1861 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     1921 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     1981 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     2041 tggtctgaca gctcgaggct tggattctca ccaataaaaa acgcccggcg gcaaccgagc
     2101 gttctgaaca aatccagatg gagttctgag gtcattactg gatctatcaa caggagtcca
     2161 agcgagctcg atatcaaatt acgccccgcc ctgccactcg tcgcagtact gttgtaattc
     2221 attaagcatt ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag
     2281 cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa
     2341 gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc
     2401 tgakacgaaa aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta
     2461 acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact
     2521 ccagagygat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact
     2581 atcccatatc accagctcac cgtctttcat tgccatacgr aattccggat gagcattcat
     2641 caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt
     2701 ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga
     2761 ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc
     2821 agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa
     2881 tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgcccgat
     2941 caactcgagt gccacctg
//
