LOCUS       sequence-484564-        3538 bp    DNA     circular SYN 25-AUG-2025
DEFINITION  pAAV (non-flex) for expressing OCaMP, an orange calcium indicator, 
            in mammalian cells and in vivo. Contains CMV promoter and RSET 
            leader sequence peptide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Aggarwal A, Baker HA, Durst CD, Chen I-W, de Chambrier P, Gonzales 
            JM, Marvin JS, Vandal M, Lundberg T, Sakoi K, Patel RH, Wang C-Y, 
            Visser F, Fouad Y, Sunil S, Wiens M, Terai T, Takahashi-Yamashiro K,
            Thompson RJ, Brown TA, Nasu Y, Nguyen MD, Gordon GRJ, McFarlane S, 
            Podgorski K, Holtmaat A, Campbell RE, Lohman AW
  TITLE     A sensitive orange fluorescent calcium ion indicator for imaging 
            neural activity
  JOURNAL   bioRxiv 2025.07.28.667269; doi: 
            https://doi.org/10.1101/2025.07.28.667269
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 25, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.07.28.667269; doi: https://doi.org/10.1101/2025.07.28.667269"
COMMENT     Sequence Label: pAAV-CMV-OCaMP-WPRE
FEATURES             Location/Qualifiers
     source          1..3538
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        201..303
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             304..963
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(370..389)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     tRNA            complement(2208..2284)
                     /gene="argU"
                     /product="E. coli tRNA-Arg recognizing AGA and AGG"
                     /label=argU
     promoter        complement(join(2528..2533,2534..2550,2551..2556))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      2668..3213
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     3114..3133
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3330..3347
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gacagctgat agaaacagaa gccactggag cacctcaaaa acaccatcat acactaaatc
       61 agtaagttgg cagcatcacc cgacgcactt tgcgccgaat aaatacctgt gacggaagat
      121 cacttcgcag aataaataaa tcctggtgtc cctgttgata ccgggaagcc ctgggccaac
      181 ttttggcgaa aatgagacgt tgatcggcac gtaagaggtt ccaactttca ccataatgaa
      241 ataagatcac taccgggcgt attttttgag ttatcgagat tttcaggagc taaggaagct
      301 aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa
      361 gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg
      421 gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt
      481 attcacattc ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac
      541 ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact
      601 gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata
      661 tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt
      721 gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac
      781 gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa
      841 ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc
      901 catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg
      961 taattttttt aaggcagtta ttggtgccct taaacgcctg gttgctacgc ctgaataagt
     1021 gataataagc ggatgaatgg cagaaattcg aaagcaaatt cgacccggtc gtcggttcag
     1081 ggcagggtcg ttaaatagcc gcttatgtct attgctggtt taccggttta ttgactaccg
     1141 gaagcagtgt gaccgtgtgc ttctcaaatg cctgaggcca gtttgctcag gctctccccg
     1201 tggaggtaat aattgacgat atgatcattt attctgcctc ccagagcctg ataaaaacgg
     1261 ttagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat
     1321 gcagatccgg aacataatgg tgcagggcgc ttgtttcggc gtgggtatgg tggcaggccc
     1381 cgtggccggg ggactgttgg gcgctgccgg cacctgtcct acgagttgca tgataaagaa
     1441 gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctaccggc
     1501 agcggtgcgg actgttgtaa ctcagaataa gaaatgaggc cgctcatggc gttgactctc
     1561 agtcatagta tcgtggtatc accggttggt tccactctct gttgcgggca acttcagcag
     1621 cacgtagggg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat
     1681 gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc
     1741 ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac
     1801 aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgactc gagggtgtgg
     1861 tctggacgtt ctgataacag aaaataaaca ggctgtgatg tgtcacggtc tgtttatcga
     1921 attaattgca gatataaaaa aaccaaccgt aagggttggt tttttcttgg gatttttggt
     1981 cggcacgaga ggatttgaac ctccgacccc cgacacccca tgacggtgcg ctaccaggct
     2041 gcgctacgtg ccgactcgtg gctgctaata ctaccgtttt ccacaccgat tgcaagtaag
     2101 atatttcgct aactgattta taattaatca gttagcgata aaacgcttct cgtctgtcag
     2161 cacccatggt ggaggatata aagaaggcgt aactgattga attgtaatgg cgcgccctgc
     2221 aggattcgaa cctgcggccc acgacttaga aggtcgttgc tctatccaac tgagctaagg
     2281 gcgcgttgat accgcaatgc ggtgtaatcg cgtgaattat acggtcaacc cttgctgagt
     2341 caatggcttg tgatctggtt gctgaacaag tgaacgaccg cgtctgatta ctagtttgcc
     2401 cgggcccttg tcgtcgtcaa ttgtacagca tccagggtga cggtgccgag gatgacgatg
     2461 agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa
     2521 ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattaca acttatatcg
     2581 tatggggctg acttcaggtg ctacatttga agagataaat tgcactgaaa tctagaaata
     2641 ttttatctga ttaataagat gatcttcttg agatcgtttt ggtctgcgcg taatctcttg
     2701 ctctgaaaac gaaaaaaccg ccttgcaggg cggtttttcg aaggttctct gagctaccaa
     2761 ctctttgaac cgaggtaact ggcttggagg agcgcagtca ccaaaacttg tcctttcagt
     2821 ttagccttaa ccggcgcatg acttcaagac taactcctct aaatcaatta ccagtggctg
     2881 ctgccagtgg tgcttttgca tgtctttccg ggttggactc aagacgatag ttaccggata
     2941 aggcgcagcg gtcggactga acggggggtt cgtgcataca gtccagcttg gagcgaactg
     3001 cctacccgga actgagtgtc aggcgtggaa tgagacaaac gcggccataa cagcggaatg
     3061 acaccggtaa accgaaaggc aggaacagga gagcgcacga gggagccgcc agggggaaac
     3121 gcctggtatc tttatagtcc tgtcgggttt cgccaccact gatttgagcg tcagatttcg
     3181 tgatgcttgt caggggggcg gagcctatgg aaaaacggct ttgccgcggc cctctcactt
     3241 ccctgttaag tatcttcctg gcatcttcca ggaaatctcc gccccgttcg taagccattt
     3301 ccgctcgccg cagtcgaacg accgagcgta gcgagtcagt gagcgaggaa gcggaatata
     3361 tcctgtatca catattctgc tgacgcaccg gtgcagcctt ttttctcctg ccacatgaag
     3421 cacttcactg acaccctcat cagtgccaac atagtaagcc agtatacact ccgctagcgc
     3481 tgatgtccgg cggtgctttt gccgttacgc accaccccgt cagtagctga acaggagg
//
