LOCUS       sequence-484591-       11288 bp    DNA     linear   UNA 26-AUG-2025
DEFINITION  Expresses the non-responsive yEPAC-R279L sensor in budding yeast..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11288)
  AUTHORS   Botman D, O'Toole TG, Goedhart J, Bruggeman FJ, van Heerden JH, 
            Teusink B
  TITLE     A yeast FRET biosensor enlightens cAMP signaling.
  JOURNAL   Mol Biol Cell. 2021 Jun 15;32(13):1229-1240. doi: 
            10.1091/mbc.E20-05-0319. Epub 2021 Apr 21.
  PUBMED    33881352
REFERENCE   2  (bases 1 to 11288)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 26, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E20-05-0319"; journalName: "Mol Biol Cell"; date: 
            "2021-06-15- 15"; volume: "32"; issue: "13"; pages: "1229-1240"
COMMENT     Sequence Label: yEPAC-R279L pDRF1-GW
FEATURES             Location/Qualifiers
     source          1..11288
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    800..824
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(835..837,838..840,841..1353)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(880..901)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1141..1160)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1488..1509
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3739..5169
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     protein_bind    complement(5178..5202)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5264..5283)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5265..5283)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      5431..5618
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(5486..5510)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(5630..5647)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5630..5646)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5639..5661)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6082..7428
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(7539..8342)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(8343..8558)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(8583..8601)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8669..8773
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8774..8842,8843..9634)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8992..9011)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9805..10393
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10294..10313
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10547..10564
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10681..10702
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10717..10722,10723..10740,10741..10747)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10755..10771
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10760..10782
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10779..10795
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10779..10795
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(10825..11280)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10962..10983)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11174..11193
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 caagcttcct gaaacggaga aacataaaca ggcattgctg ggatcaccca tacatcactc
       61 tgttttgcct gaccttttcc ggtaatttga aaacaaaccc ggtctcgaag cggagatccg
      121 gcgataatta ccgcagaaat aaacccatac acgagacgta gaaccagccg cacatggccg
      181 gagaaactcc tgcgagaatt tcgtaaactc gcgcgcattg catctgtatt tcctaatgcg
      241 gcacttccag gcctcgatcg agaccgttta tccattgctt ttttgttgtc tttttccctc
      301 gttcacagaa agtctgaaga agctatagta gaactatgag ctttttttgt ttctgttttc
      361 cttttttttt tttttacctc tgtggaaatt gttactctca cactctttag ttcgtttgtt
      421 tgttttgttt attccaatta tgaccggtga cgaaacgtgg tcgatggtgg gtaccgctta
      481 tgctcccctc cattagtttc gattatataa aaaggccaaa tattgtatta ttttcaaatg
      541 tcctatcatt atcgtctaac atctaatttc tcttaaattt tttctctttc tttcctataa
      601 caccaatagt gaaaatcttt ttttcttcta tatctacaaa aacttttttt ttctatcaac
      661 ctcgttgata aattttttct ttaacaatcg ttaataatta attaattgga aaataaccat
      721 tttttctctc ttttatacac acattcaaaa gaaagaaaaa aaatataccc cagcctcgac
      781 tagtgaattc gcggccatca caagtttgta caaaaaagca ggctgctagc cgcaatggtg
      841 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
      901 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
      961 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     1021 accaccctgt cctggggcgt gcagtgcttc gcccgctacc ccgaccacat gaagcagcac
     1081 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     1141 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     1201 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     1261 gagtacaact actttagcga caacgtctat atcaccgccg acaagcagaa gaacggcatc
     1321 aaggccaact tcaagatccg ccacaacatc gaggacggcg gcgtgcagct cgccgaccac
     1381 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     1441 agcacccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     1501 gagttcgtga ccgccgcccg atatcagccc gtgggaactc atgagatgga ggaggagttg
     1561 gccgaagctg tggccctgct ctcccagcgg gggcctgacg ccctgctcac tgtggcactt
     1621 cgaaagcccc caggtcagcg cacggatgaa gagctggacc tcatctttga ggagctgctg
     1681 cacatcaagg ctgtggccca cctctccaac tcggtgaagc gagaattagc ggctgttctg
     1741 ctctttgaac cacacagcaa ggcagggacc gtgttgttca gccaggggga caagggcact
     1801 tcgtggtaca ttatctggaa gggatctgtc aacgtggtga cccatggcaa ggggctggtg
     1861 accaccctgc atgagggaga tgattttgga cagctggctc tggtgaatga tgcacccctg
     1921 gcagccacca tcatcctgcg agaagacaac tgtcatttcc tgcgtgtgga caagcaggac
     1981 ttcaaccgta tcatcaagga tgtggaggca aagaccatgc ggctggaaga acatggcaaa
     2041 gtggtgctgg tgctggagag agcctctcag ggcgccggcc cttcccgacc cccaacccca
     2101 ggcaggaacc ggtatacagt gatgtctggc accccagaga agatcctaga gcttctgttg
     2161 gaggccatgg gaccagattc cagtgctcat gacccaacag agacattcct cagcgacttc
     2221 ctcctgaccc acagggtctt catgcccagc gcccaactct gcgctgccct tctgcaccac
     2281 ttccatgtgg agcctgcggg tggcagcgag caggagcgca gcacctacgt ctgcaacaag
     2341 aggcagcaga tcttgcggct ggtcagccag tgggtggccc tgtatggctc catgctccac
     2401 actgaccctg tggccaccag cttcctccag aaactctcag acctggtggg cagggacacc
     2461 cgactcagca acctgctgag ggagcagtgg ccagagaggc ggcgatgcca caggttggag
     2521 aatggctgtg ggaatgcatc tcctcagatg aaggcccgga acttgcctgt ttggctcccc
     2581 aaccaggacg agccccttcc tggcagcagc tgtgccatcc aagttgggga taaagtcccc
     2641 tatgacatct gccggccaga ccactcagtg ttgaccctgc agctgcctgt gacagcctcc
     2701 gtgagagagg tgatggcagc gttggcccag gaggatggct ggaccaaggg gcaggtgctg
     2761 gtgaaggtca attctgcagg tgatgccatt ggcctgcagc cagatgcccg tggtgtggcc
     2821 acatctctgg ggctcaatga gcgtctcttt gttgtcaacc cacaggaagc gcatgagctg
     2881 atcccacacc ctgaccagct ggggcccact gtgggctctg ctgaggggct ggacctggtg
     2941 agtgccaagg acctggcagg ccagctgacg gaccacgact ggagcctctt caacagtatc
     3001 caccaggtgg agctgatcca ctatgtgctg ggcccccagc atctgcggga tgtcaccacc
     3061 gccaacctgg agcgcttcat gcgccgcttc aatgagctgc agtactgggt ggccaccgag
     3121 ctgtgtctct gccccgtgcc cggcccccgg gcccagctgc tcaggaagtt cattaagctg
     3181 gcggcccacc tcaaggagca gaagaatctc aattccttct ttgccgtcat gtttggcctc
     3241 agcaactcgg ccatcagccg cctagcccac acctgggagc ggctgcccca caaagtccgg
     3301 aagctgtact ccgccctcga gaggctgctg gatccctcat ggaaccaccg ggtataccga
     3361 ctggccctcg ccaagctctc ccctcctgtc atccccttca tgccccttct tctcaaagac
     3421 atggccgcca ttcatgaggg aaaccacaca ctagtggaga atctcatcaa ctttgagaag
     3481 atgagaatga tggccagagc cgcgcggatg ctgcaccact gccgaagcca caaccctgtg
     3541 cctctctcac cactcagaag ccgagtttcc cacctccacg aggacagcca ggtggcgagg
     3601 atttccacat gctcggagca gtccctgagc acccggagtc cagccagcac ctgggcttat
     3661 gtccagcagc tgaaggtcat tgacaaccag cgggaactct cccgcctctc ccgagagctg
     3721 gagccagcaa gcgagctcat ggtgagcaag ggcgaggagg tcatcaaaga gttcatgcgc
     3781 ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc
     3841 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc
     3901 ctgcccttcg cctgggacat cctgtccccc cagttcatgt acggctccaa ggcgtacgtg
     3961 aagcaccccg ccgacatccc cgattacaag aagctgtcct tccccgaggg cttcaagtgg
     4021 gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg tgacccagga ctcctccctg
     4081 caggacggca cgctgatcta caaggtgaag atgcgcggca ccaacttccc ccccgacggc
     4141 cccgtaatgc agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc
     4201 gacggcgtgc tgaagggcga gatccaccag gccctgaagc tgaaggacgg cggccactac
     4261 ctggtggagt tcaagaccat ctacatggcc aagaagcccg tgcaactgcc cggctactac
     4321 tacgtggaca ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag
     4381 tacgagcgct ccgagggccg ccaccacctg ttcctggggc atggcaccgg cagcaccggc
     4441 agcggcagct ccggcaccgc ctcctccgag gacaacaaca tggccgtcat caaagagttc
     4501 atgcgcttca aggtgcgcat ggagggctcc atgaacggcc acgagttcga gatcgagggc
     4561 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc
     4621 ggccccctgc ccttcgcctg ggacatcctg tccccccagt tcatgtacgg ctccaaggcg
     4681 tacgtgaagc accccgccga catccccgat tacaagaagc tgtccttccc cgagggcttc
     4741 aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc
     4801 tccctgcagg acggcacgct gatctacaag gtgaagatgc gcggcaccaa cttccccccc
     4861 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga gcgcctgtac
     4921 ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa ggacggcggc
     4981 cactacctgg tggagttcaa gaccatctac atggccaaga agcccgtgca actgcccggc
     5041 tactactacg tggacaccaa gctggacatc acctcccaca acgaggacta caccatcgtg
     5101 gaacagtacg agcgctccga gggccgccac cacctgttcc tgtacggcat ggacgagctg
     5161 tacaagtaag cggccgcacc cagctttctt gtacaaagtg gtgtagttaa ttcatgatgg
     5221 ccgctgcagg tcgacctcga gggggggccc ggtacccaat tcgccctata gtgagtcgta
     5281 ttacgcgcgg atccagcttt ggacttcttc gccagaggtt tggtcaagtc tccaatcaag
     5341 gttgtcggct tgtctacctt gccagaaatt tacgaaaaga tggaaaaggg tcaaatcgtt
     5401 ggtagatacg ttgttgacac ttctaaataa gcgaatttct tatgatttat gatttttatt
     5461 attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta
     5521 aaacganaat tcttattctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta
     5581 tagcatgagg tcgctcttat tgaccacacc tctaccggca tgccaattca ctggccgtcg
     5641 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     5701 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     5761 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
     5821 gcggtatttc acaccgcata atcggatcgt acttgttacc catcattgaa ttttgaacat
     5881 ccgaacctgg gagttttccc tgaaacagat agtatatttg aacctgtata ataatatata
     5941 gtctagcgct ttacggaaga caatgtatgt atttcggttc ctggagaaac tattgcatct
     6001 attgcatagg taatcttgca cgtcgcatcc ccggttcatt ttctgcgttt ccatcttgca
     6061 cttcaatagc atatctttgt taacgaagca tctgtgcttc attttgtaga acaaaaatgc
     6121 aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa
     6181 tgcaacgcga aagcgctatt ttaccaacga agaatctgtg cttcattttt gtaaaacaaa
     6241 aatgcaacgc gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac
     6301 agaaatgcaa cgcgagagcg ctattttacc aacaaagaat ctatacttct tttttgttct
     6361 acaaaaatgc atcccgagag cgctattttt ctaacaaagc atcttagatt actttttttc
     6421 tcctttgtgc gctctataat gcagtctctt gataactttt tgcactgtag gtccgttaag
     6481 gttagaagaa ggctactttg gtgtctattt tctcttccat aaaaaaagcc tgactccact
     6541 tcccgcgttt actgattact agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc
     6601 ccgattatat tctataccga tgtggattgc gcatactttg tgaacagaaa gtgatagcgt
     6661 tgatgattct tcattggtca gaaaattatg aacggtttct tctattttgt ctctatatac
     6721 tacgtatagg aaatgtttac attttcgtat tgttttcgat tcactctatg aatagttctt
     6781 actacaattt ttttgtctaa agagtaatac tagagataaa cataaaaaat gtagaggtcg
     6841 agtttagatg caagttcaag gagcgaaagg tggatgggta ggttatatag ggatatagca
     6901 cagagatata tagcaaagag atacttttga gcaatgtttg tggaagcggt attcgcaata
     6961 ttttagtagc tcgttacagt ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc
     7021 gcttttggtt ttcaaaagcg ctctgaagtt cctatacttt ctagctagag aataggaact
     7081 tcggaatagg aacttcaaag cgtttccgaa aacgagcgct tccgaaaatg caacgcgagc
     7141 tgcgcacata cagctcactg ttcacgtcgc acctatatct gcgtgttgcc tgtatatata
     7201 tatacatgag aagaacggca tagtgcgtgt ttatgcttaa atgcgtactt atatgcgtct
     7261 atttatgtag gatgaaaggt agtctagtac ctcctgtgat attatcccat tccatgcggg
     7321 gtatcgtatg cttccttcag cactaccctt tagctgttct atatgctgcc actcctcaat
     7381 tggattagtc tcatccttca atgctatcat ttcctttgat attggatcga tccgatgata
     7441 agctgtcaaa catgagaatt gggtaataac tgatataatt aaattgaagc tctaatttgt
     7501 gagtttagta tacatgcatt tacttataat acagtttttt agttttgctg gccgcatctt
     7561 ctcaaatatg cttcccagcc tgcttttctg taacgttcac cctctacctt agcatccctt
     7621 ccctttgcaa atagtcctct tccaacaata ataatgtcag atcctgtaga gaccacatca
     7681 tccacggttc tatactgttg acccaatgcg tctcccttgt catctaaacc cacaccgggt
     7741 gtcataatca accaatcgta accttcatct cttccaccca tgtctctttg agcaataaag
     7801 ccgataacaa aatctttgtc gctcttcgca atgtcaacag tacccttagt atattctcca
     7861 gtagataggg agcccttgca tgacaattct gctaacatca aaaggcctct aggttccttt
     7921 gttacttctt ctgccgcctg cttcaaaccg ctaacaatac ctgggcccac cacaccgtgt
     7981 gcattcgtaa tgtctgccca ttctgctatt ctgtatacac ccgcagagta ctgcaatttg
     8041 actgtattac caatgtcagc aaattttctg tcttcgaaga gtaaaaaatt gtacttggcg
     8101 gataatgcct ttagcggctt aactgtgccc tccatggaaa aatcagtcaa gatatccaca
     8161 tgtgttttta gtaaacaaat tttgggacct aatgcttcaa ctaactccag taattccttg
     8221 gtggtacgaa catccaatga agcacacaag tttgtttgct tttcgtgcat gatattaaat
     8281 agcttggcag caacaggact aggatgagta gcagcacgtt ccttatatgt agctttcgac
     8341 atgatttatc ttcgtttcct gcatgttttt gttctgtgca gttgggttaa gaatactggg
     8401 caatttcatg tttcttcaac actacatatg cgtatatata ccaatctaag tctgtgctcc
     8461 ttccttcgtt cttccttctg ttcggagatt accgaatcaa aaaaatttca aggaaaccga
     8521 aatcaaaaaa aagaataaaa aaaaaatgat gaattgaaaa gctaattctt gaagacgaaa
     8581 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     8641 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     8701 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     8761 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     8821 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     8881 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     8941 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     9001 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     9061 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     9121 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     9181 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     9241 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     9301 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     9361 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     9421 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     9481 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     9541 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     9601 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     9661 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     9721 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     9781 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     9841 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     9901 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     9961 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
    10021 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
    10081 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
    10141 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
    10201 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
    10261 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
    10321 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
    10381 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
    10441 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
    10501 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
    10561 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
    10621 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
    10681 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
    10741 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
    10801 ttacgccaag cttaccgcat caggaaattg taagcgttaa tattttgtta aaattcgcgt
    10861 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
    10921 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
    10981 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
    11041 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
    11101 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
    11161 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
    11221 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
    11281 ccattcgc
//
