LOCUS       sequence-484592-        1231 bp    DNA     linear   UNA 26-AUG-2025
DEFINITION  Expresses mTq2 in budding yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1231)
  TITLE     mTurquoise2 pDRF1-GW
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1231)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 26, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: mTurquoise2 pDRF1-GW
FEATURES             Location/Qualifiers
     source          1..1231
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    89..113
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      130..139
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(136..138,139..141,142..855)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(181..202)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(442..461)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     789..810
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(864..888)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(950..969)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(951..969)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gttagcattt ttttctctct tttatacaca cattcaaaag aaagaaaaaa aatatacccc
       61 agcctcgact agtgaattcg cggccatcac aagtttgtac aaaaaagcag gctgctagcg
      121 ctaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
      181 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
      241 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
      301 gtgccctggc ccaccctcgt gaccaccctg tcctggggcg tgcagtgctt cgcccgctac
      361 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
      421 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
      481 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
      541 aacatcctgg ggcacaagct ggagtacaac tactttagcg acaacgtcta tatcaccgcc
      601 gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat cgaggacggc
      661 ggcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
      721 ctgcccgaca accactacct gagcacccag tccaagctga gcaaagaccc caacgagaag
      781 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
      841 gagctgtaca agtaagcggc cgcacccagc tttcttgtac aaagtggtgt agttaattca
      901 tgatggccgc tgcaggtcga cctcgagggg gggcccggta cccaattcgc cctatagtga
      961 gtcgtattac gcgcggatcc agctttggac ttcttcgcag aggtttggtc agtctcatca
     1021 gtgtcggctg tctacctgca gaatttacga aagatggaag gtcaatcgtg tagatacgtg
     1081 tgacacttct aataagcgat tctatgatta tgattttata ttaaataagt taaaaaaaaa
     1141 tagcgttata caaagttagg tgaactctaa gctttaacga attctatctg gaacgttctg
     1201 agtcaggtct ctccggtagc ctgagggccc c
//
