LOCUS       sequence-484639-       29440 bp    DNA     circular UNA 26-AUG-2025
DEFINITION  PVC structural operon - untargeted (truncated) tail fiber (Pvc13) - 
            spike tip (Pvc10) loaded with Cas9 on CTD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 29440)
  TITLE     Feng Zhang Lab - PVC plasmids
REFERENCE   2  (bases 1 to 29440)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 26, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAWP78-PVCpnf_pvc13-Truncated_pvc10-Cas9
FEATURES             Location/Qualifiers
     source          1..29440
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(339..1154)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(339..1154)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1062..1081
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1146..1165
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             complement(1458..2606)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(2878..3249)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            3282..3391
                     /note="incP origin of transfer"
     rep_origin      3544..4174
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      4483..5071
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4972..4991
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             13740..13760
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             13785..17888
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             13785..17888
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 gagatgatga taagaatggt cagtcaccag ataaaatgat ctctggtcaa ttttaggaaa
       61 gctgaaaaaa cctgaccaaa tgcgtcgtaa attctatgct tagaaagact cggtgccagt
      121 gtaataccta agatatggtt tttatttcca tatctgttcg aaaacttaga aataacaagt
      181 tcaacaataa ccagggactt taaatatcgg atccttgtcg ggaagatgcg tgatctgatc
      241 cttcaactca gcaaaagttc gatttattca acaaagccgc cgtcccgtca agtcagcgta
      301 atgctctgcc agtgttacaa ccaattaacc aattctgatt agaaaaactc atcgagcatc
      361 aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt
      421 ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat
      481 cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa
      541 ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa
      601 agcttatgca tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa
      661 tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg
      721 cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact
      781 gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct
      841 gttttcccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc
      901 ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta
      961 acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc
     1021 ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac
     1081 ccatataaat cagcatccat gttggaattt aatcgcggcc tcgagcaaga cgtttcccgt
     1141 tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt
     1201 catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga cacaacgtgg
     1261 ctttcgactt ccggcaagct atacgcgccc tagaattgtc aattttaatc ctctgtttat
     1321 cggcagttcg tagagcgcgc cgtgcgtccc gagcgatact gagcgaagca agtgcgtcga
     1381 gcagtgcccg cttgttcctg aaatgccagt aaagcgctgg ctgctgaacc cccagccgga
     1441 actgacccca caaggcccta gcgtttgcaa tgcaccaggt catcattgac ccaggcgtgt
     1501 tccaccaggc cgctgcctcg caactcttcg caggcttcgc cgacctgctc gcgccacttc
     1561 ttcacgcggg tggaatccga tccgcacatg aggcggaagg tttccagctt gagcgggtac
     1621 ggctcccggt gcgagctgaa atagtcgaac atccgtcggg ccgtcggcga cagcttgcgg
     1681 tacttctccc atatgaattt cgtgtagtgg tcgccagcaa acagcacgac gatttcctcg
     1741 tcgatcagga cctggcaacg ggacgttttc ttgccacggt ccaggacgcg gaagcggtgc
     1801 agcagcgaca ccgattccag gtgcccaacg cggtcggacg tgaagcccat cgccgtcgcc
     1861 tgtaggcgcg acaggcattc ctcggccttc gtgtaatacc ggccattgat cgaccagccc
     1921 aggtcctggc aaagctcgta gaacgtgaag gtgatcggct cgccgatagg ggtgcgcttc
     1981 gcgtactcca acacctgctg ccacaccagt tcgtcatcgt cggcccgcag ctcgacgccg
     2041 gtgtaggtga tcttcacgtc cttgttgacg tggaaaatga ccttgttttg cagcgcctcg
     2101 cgcgggattt tcttgttgcg cgtggtgaac agggcagagc gggccgtgtc gtttggcatc
     2161 gctcgcatcg tgtccggcca cggcgcaata tcgaacaagg aaagctgcat ttccttgatc
     2221 tgctgcttcg tgtgtttcag caacgcggcc tgcttggcct cgctgacctg ttttgccagg
     2281 tcctcgccgg cggtttttcg cttcttggtc gtcatagttc ctcgcgtgtc gatggtcatc
     2341 gacttcgcca aacctgccgc ctcctgttcg agacgacgcg aacgctccac ggcggccgat
     2401 ggcgcgggca gggcaggggg agccagttgc acgctgtcgc gctcgatctt ggccgtagct
     2461 tgctggacca tcgagccgac ggactggaag gtttcgcggg gcgcacgcat gacggtgcgg
     2521 cttgcgatgg tttcggcatc ctcggcggaa aaccccgcgt cgatcagttc ttgcctgtat
     2581 gccttccggt caaacgtccg attcattcac cctccttgcg ggattgcccc gactcacgcc
     2641 ggggcaatgt gcccttattc ctgatttgac ccgcctggtg ccttggtgtc cagataatcc
     2701 accttatcgg caatgaagtc ggtcccgtag accgtctggc cgtccttctc gtacttggta
     2761 ttccgaatct tgccctgcac gaataccagc tccgcgaagt cgctcttctt gatggagcgc
     2821 atggggacgt gcttggcaat cacgcgcacc ccccggccgt tttagcggct aaaaaagtca
     2881 tggctctgcc ctcgggcgga ccacgcccat catgaccttg ccaagctcgt cctgcttctc
     2941 ttcgatcttc gccagcaggg cgaggatcgt ggcatcaccg aaccgcgccg tgcgcgggtc
     3001 gtcggtgagc cagagtttca gcaggccgcc caggcggccc aggtcgccat tgatgcgggc
     3061 cagctcgcgg acgtgctcat agtccacgac gcccgtgatt ttgtagccct ggccgacggc
     3121 cagcaggtag gcctacaggc tcatgccggc cgccgccgcc ttttcctcaa tcgctcttcg
     3181 ttcgtctgga aggcagtaca ccttgatagg tgggctgccc ttcctggttg gcttggtttc
     3241 atcagccatc cgcttgccct catctgttac gccggcggta gccggccagc ctcgcagagc
     3301 aggattcccg ttgagcaccg ccaggtgcga ataagggaca gtgaagaagg aacacccgct
     3361 cgcgggtggg cctacttcac ctatcctgcc cggctgacgc cgttggatac accaaggaaa
     3421 gtctacacga accctttggc aaaatcctgt atatcgtgcg aaaaaggatg gatataccga
     3481 aaaaatcgct ataatgaccc cgaagcaggg ttatgcagcg gaaaagatcc gtcgaccctt
     3541 tccgacgctc accgggctgg ttgccctcgc cgctgggctg gcggccgtct atggccctgc
     3601 aaacgcgcca gaaacgccgt cgaagccgtg tgcgagacac cgcggccgcc ggcgttgtgg
     3661 atacctcgcg gaaaacttgg ccctcactga cagatgaggg gcggacgttg acacttgagg
     3721 ggccgactca cccggcgcgg cgttgacaga tgaggggcag gctcgatttc ggccggcgac
     3781 gtggagctgg ccagcctcgc aaatcggcga aaacgcctga ttttacgcga gttttccaca
     3841 gatgatgtgg acaagcctgg ggataagtgc cctgcggtat tgacacttga ggggcgcgac
     3901 tactgacaga tgaggggcgc gatccttgac acttgagggg cagagtgctg acagatgagg
     3961 ggcgcaccta ttgacatttg aggggctgtc cacaggcaga aaatccagca tttgcaaggg
     4021 tttccgcccg tttttcggcc accgctaacc tgtcttttaa cctgctttta aaccaatatt
     4081 tataaacctt gttttaacca gggctgcgcc ctgtgcgcgt gaccgcgcac gccgaagggg
     4141 ggtgcccccc cttctcgaac cctcccggcc cgctaacgcg ggcctcccat ccccccaggg
     4201 gctgcgcccc tcggccgcga acggcctcac cccaaaaatg gcagccaagc tgacccgcta
     4261 gggacgtgaa gtcgattcct tcgatggtta gcaatcaaag actcagagtg ctgtctggag
     4321 cgtgaatcta acggtacgta tctcgattgc tcggtcgcta ttcgcactct gcgaaagttc
     4381 gtaccgctca ttcactaggt tgcgaatcat gaccaaaatc ccttaacgtg agttttcgtt
     4441 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     4501 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     4561 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     4621 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     4681 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     4741 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     4801 aacggggggt tcgtgcacac agcccagctt gtagcgaacg acctacaccg aactgagata
     4861 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     4921 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     4981 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     5041 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     5101 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     5161 ggataaccgt attaccgcct ttgagtgagc tgtcgcgaat gcatctagat ataattacat
     5221 cttcatcatt cggtatttta aatgacatat atcctccttc cgaatagttt taatggtgat
     5281 gttcgattgg acagtgtttt ccattattaa aattttaagt atcacatcat gtttaatgaa
     5341 ggattgatgt ttattagaat attcataata gctttgtctt tgcctctatg taaatgaaat
     5401 tgcataaaaa gcaaattgca ttattggcaa tgtacttatt ctggattttt cggcacgata
     5461 aattattggt gtaagtttta tatcatgagt aaaaattcgg aatgatattc ctgtcatttt
     5521 gtggcggtag cgttactcaa gatattatcc tacatcatat gttgattatt gtaatcaatc
     5581 gagctatttt ttgtctaaaa aggggctttt atgtctacaa gtacatctca aattgcggtt
     5641 gaatatccta ttcctgtcta tcgctttatt gtttctgtcg gagatgagaa aattccattt
     5701 aatagtgttt caggattaga tattagttat gacaccattg aataccgaga tggtgttggt
     5761 aattggttca aaatgccggg tcagagtcag agcactaata tcaccttgcg taaaggcgtt
     5821 ttcccgggga aaacagaact gtttgattgg attaactcta ttcagcttaa tcaggtagag
     5881 aaaaaggata ttaccatcag tttaactaat gatgcaggta ccgaattatt aatgacctgg
     5941 aatgtttcta atgcttttcc cacttcattg acttcacctt catttgatgc caccagtaat
     6001 gatattgcag tacaggaaat tacgctgatg gcagatcggg tgattatgca ggctgtttga
     6061 agcattgata tttaatcatc tcatataagg gaacttttat gacaaccgtt accagttatc
     6121 ctggcgttta tattgaagaa ttaaatagcc tggccttgtc agtttcaaat agcgccacag
     6181 cggttcctgt ttttgctgtg gacgaacaaa accaatatat tagtgaagat aatgcaatcc
     6241 gtattaattc gtggatggat tatcttaatc tgattggcaa ttttaataat gaagacaaat
     6301 tagatgtttc tgtgcgtgct tattttgcca atggaggtgg atattgttat ctcgtcaaaa
     6361 caacgagttt agaaaaaatt attccaacct tggatgatgt aaccttattg gttgctgcgg
     6421 gcgaagatat taaaacgaca gtagatgttt tatgtcagcc aggaaaaggg ttattcgcag
     6481 tctttgatgg ccctgaaaca gagttgacta tcaacggtgc ggaagaggca aaacaagcct
     6541 ataccgccac accattcgct gcggtttatt atccttggtt gaaagcggat tgggctaaca
     6601 tagatattcc acccagtgca gtgatggcgg gagtttatgc atcggtggat ttatcccgtg
     6661 gtgtatggaa agcgcctgcc aatgttgcgt tgaaaggggg cctggaacct aaatttttag
     6721 tcacggatga attgcagggt gaatataaca ctggccgcgc tatcaatatg attcgtaatt
     6781 tcagtaacac aggtactacg gtttggggtg caagaaccct ggaagataaa gacaattggc
     6841 gttatgttcc agtgcgacgc ttgtttaatt ctgtggagcg ggatatcaag cgtgccatga
     6901 gctttgctat gttcgagcct aataatcagc ccacttggga gcgggtacgg gcggcgatta
     6961 gcaactacct ttatagcctg tggcaacagg ggggattagc tggcagcaaa gaagaagacg
     7021 cttattttgt gcaaattggt aaaggtataa cgatgacaca ggagcagatt gatgcagggc
     7081 aaataattgt taaagtcggt ttggctgctg tacggcctgc ggaatttatc attctccagt
     7141 ttacgcaaga tgtagaacag cgttaatcat atgattatga ggagttatca tgtctgctat
     7201 tctgaaagcg cctggcgttt atattgaaga agacgcttcc ctagcgttgt ctgtcagtaa
     7261 cagcgcgact gccgtgcctg tttttatcgg aaaatttact ccgacagtgg ttgattcaat
     7321 ccaagtctgt acccgtatca gcaactggct tgaattcact tcctcttttt ccctagctcc
     7381 aacagttgag attgttgtcc aatctaacac tgaatctgaa tctgaatctg aaacttacca
     7441 ctatattgag acaatcaatt tatctccagc tgtggaagca ttgcgactct attttcaaaa
     7501 tggcggagga gcttgctata tctacccatt aaatgatgct gaagatgaat tggttctggc
     7561 ggccatacca gaagtcattg aacagaaagg tgatattact ctgttggttt gcccggaact
     7621 cgatctggat tacaaaacta agatctatgg cgcagtgagc tcactgttga atgataacaa
     7681 agtgggctat ttcctgattg cggatagcaa tgatggagaa tctgtgtcag gagtatggaa
     7741 tagtgctaag gccgccgcct attatcccca gttggaaact aacctaaaat tttccacgtt
     7801 gcctggggat aaggacattc gtatcagcgg ttatcaggat gatgatgaaa cacataaacc
     7861 gaaaaacttg gatgagctca ggacaatcaa cgaggcgttg gcacaggata ttgatgcaag
     7921 attgctcgag gagaaacaac gtgctgtcat cattccgcca agtgctgcca ttgcgggcat
     7981 ttattgccaa acggataatc gtcgcggtgt ttggaaagcg ccagccaacg ttgcgctcac
     8041 agggatcggg agtttgcttg ataaggtaga cgatgaacgg cagggagaga tgaatgacaa
     8101 gggaatcaat gtcatccgtt catttaccga ccgtggtttt atggtctggg gagcccgtac
     8161 ttgtgtggac gctgccaaca tcagctggcg ttatattcct gttcgtcgcc tgttcaattc
     8221 cgttgaacga gatatccgcc aggcgctgcg cgctgtgttg tttgaaacta atagtcagcc
     8281 tacctgggta cgtgctaagg ctgccgttga tcaatatctt tatacccttt ggcagaaaaa
     8341 tgcattgatg ggtgctcgcc cggaagaagc ttattttgtg caaattggtc aggatatcac
     8401 catgtccgag gctgatatta aacagggtaa gatgatcatg actgttggtt tggcagcagt
     8461 gcggccagct gagttcatca ttctgcaatt tacgcaggat gttgttcagt aatctccatg
     8521 actaaacgcc aggcactgta ttgacagtgc ctactctaac catcttggag gaggtgatga
     8581 tgatggagag actccaaccg ggtgtgactt taacagaaag tataatcacg atgggtcagc
     8641 aagagatacc cagtgctgtg ccggtgttta ttggttacac cgttcgttat ccggaacaat
     8701 cggaagcatc agtccgtatc gacagtttgg ccgagtatac cagcctgttt ggtgacgacc
     8761 atgtgatgat gtttgctgtc aggcactatt ttgataatgg cgggcaacag gcatttgttt
     8821 tacccctgaa ggacaatatg ccatcagtgg agatgaccac agctgaagcg gaaaatctga
     8881 tagccgcatt gcgctctgct acggttagcg aagccattgg tgggcatagt cagattacac
     8941 tgattttggt accggatatg gctcggctta atgacagtga tattgatgac tcctcaaccc
     9001 aggtaagcct gtggtcccaa ggctgggagg cgctgctgca attgagtcag gttaggccca
     9061 acctctttgt gctgttagat gcgccggata atgttgaaca ggcgcagaag tgtatgacaa
     9121 cgctatcgtc agattatcgt caatgggggg cagcatattg gcctcgtctg gaaactacct
     9181 atcagaaaga aatatctggc aaggacaatg aatctcaggg aattttccag gggactgttc
     9241 tgtcacccac agccgcggtc gcagcggtaa ttcaacgcac ggataacgac gcgggtgttt
     9301 ggaaagcacc ggccaatatt gccttatccc aggttattcg acctgttaaa tcttatcttc
     9361 agggaagtgt actgtttaac agcagcggca cttcgctcaa tgtgatccgc agtttcccag
     9421 gtaagggcat acgggtatgg ggatgccgca ctctggaaaa cacggataat acgcagtggc
     9481 gctatctgca aacacgtcgg ctggtttcct atgtaacagc gcatttgacc caattggctc
     9541 gcatgtatgt cttcgagcca aataatgaac ttacctggat gaagttaaaa ggacaaagtt
     9601 acaactggtt acggcaatta tggttgcagg gtggcttgta tggttcacag gaggatgagg
     9661 catttaacat tctgttaggc gtaaacgaga cgatgactga ggatgatgtt cgtgcaggaa
     9721 aaatgatcat gaaagttgag ttggctgtgt tgtttcctgc cgaatttatt gagatcagtt
     9781 tggtgtttaa tacccaaaca gaggcgctgt cttaagaagg aaaaagtacg atgaacgatt
     9841 attacacacc cgtggtatcc catcgtttta tggcgagttt tatttttaac cgcattcccg
     9901 atccgctgga tattcgtttt cagcgtatct ctggccttag tcgggaacta caggtgactc
     9961 agtacagtga gggaggagaa aatgcccgta ataactattt agctgagaaa atccaacacg
    10021 gtacgttgac tttggaacgg ggcgtgatga cagtctcgcc attgacctgg atgtttgatc
    10081 gggtattgag tggtgaaaaa atcgcttatg ccgatgtggt ggtgatgcta ctgaatgaaa
    10141 attcactgcc attgtccagt tggacgttga gcaatgcgct gccggtacgc tggcaaacca
    10201 gcgactttga cgctaacagc aatgccatat tggtgaatac ccttgaattg cgttaccagg
    10261 atatgcgctg gcttggagtc aaaatatgac agtagaaatc agagagttac ttatccaggc
    10321 aaaggtagtg ccatcaacac gaccgactga atcagaacgg caaaaccatt ctttgataca
    10381 ggaaagtctg gatgaggcga cttgggtgga aacgataaaa cgcgaagtgt tggccgcatt
    10441 acgcgatgag gaagggtggc gtccatgagt ctgattgaac gtggtttagc taagctgaca
    10501 attaatgctt ataaggatag ggaagggaag atacgggcag gaacgttgca ggccatgtat
    10561 aaccctgact ccttgcaact ggattaccaa acggattatc agcaatccca agcgattaat
    10621 agcgaaaagc aaagtagcat ttatgtacag gccaagcccg cagggttatc acttgaatta
    10681 atttttgatg ccacgatgcc gggtaacaaa acccccattg aagagcagct catgcagctc
    10741 aagcaactgt gcagtgtgga tgcaaccagt aacgagacgc gattcctgca agttaaatgg
    10801 ggcaaaatgc gttgggaaag tcggggttac tttgctggca gggccaagag tttgtctgtg
    10861 aattacactt tgtttgatcg tgatgcgact cccttgaggg tacgggtaat attggcatta
    10921 gtggctgatg aaagtctggt gttgcaggag actgaacaaa atctgcaatc tccggcaaaa
    10981 atcgcattac gcatacagga tggggtatct ctggctctga tggcagccag tacggcatca
    11041 acattgtcag gcggtgtgga ttatctgacg ctggcctggc aaaacggtct ggataatctc
    11101 aatgggttcg ttccgggtga aatattgcag gccaccaggg gagacgaatc atgagccacc
    11161 aactgaaaat tattgcagat ggtaaggcac tgtcactttt ggccgcggta gatgtggaca
    11221 cctgttatcg ggttaacagt ataccttctg cgacattgaa actgagcgta ccggataggc
    11281 cactctcttc tttcagtcag acggatgttc agacagaact ggcccactgt caggtaggga
    11341 aaaccctgcg tctggaattg attgatggta gcaaaaaatg ggtgctgttt aatggtctta
    11401 ttacccgtaa ggctctgaga attaagaata agcaattatt gctcactctg gttgtcaagc
    11461 atcggttgca actgatggtg gatacccagc attcacagct gtttaaagac aaaagcgaaa
    11521 aagcgatctt aagcacgcta ttgaatcaga ccggaatcaa tgctcgcttc ggaaagatag
    11581 cggcgttaga tcaaaagcat gaacagatgg tgcaatttcg ttgttcagac tggcattttc
    11641 tgttgtgccg actgtcggca accggtgcat ggttgttacc tgccatagaa gacgttcagt
    11701 ttgttcaacc tgatgctctg aaatcaaact cagcctatac cttgaagagc aggggggatg
    11761 agaacaaaga catcgttgtc aaggatgctt actggcagtt tgacaatcaa atcaaccccg
    11821 ctttgctgga agtcagtggc tgggatatca gtaagcagca ggtacaatca ggcggtcgct
    11881 acggaaaaat cgcgttgggt aaggcggcac tctctcctga tggattggca tcccttaata
    11941 aaacgggttg ggacatttgt tatagcagtc cgttaacaac ccaggaaagc ggttatctgg
    12001 cacagggatt attgcttaac cagcgcattt ctggggtgac aggagaattt ttgctcaaag
    12061 gagatgggcg ttaccagttg ggagacaaca ttcagctgac tggatttggt tcacagttag
    12121 atggtacggc aagcattact gaggttcgcc accgtcttaa tcggcgaatt gattgggaaa
    12181 ccacggtgag cattggttta caacatgaat atttgccgat attacctgat gctcccgaac
    12241 tacatattgc gacagtagcg aaatatcagc aggacagtgc ggtgttaaac cgtatcccca
    12301 ttattctgcc ggtactgaat cgtcccaatg aatttttgtg ggccagattg gggaaacctt
    12361 atgctagcca tgaaagcggt ttctgttttt acccagagcc aggtgacgaa gttattattg
    12421 gtttttttga aaatgatccg cgttatccag ttattttagg tgctatgcat aatccgaaaa
    12481 ataaggcccc ttttgaacca acccaagata atagggaaaa agtattgatc gttaaaaaag
    12541 gtgaagcgca acaacaatta gtcattgatg gcaaagagaa aatgatccga attaatgcgg
    12601 gtgaaaatca aataatgctt cagcaagata aagacatttc tctgtcaacg aaaaaagaat
    12661 taacactgaa agcgcagaca atgaatgcca cgatggataa atcattggca atgtccggga
    12721 aaaacagtgt tgaaatcaaa ggcgcaaaaa ttaatcttac ccaatgaaag gtgacgatga
    12781 atggaaaatc aaatactgac acaactctat ggtcgtggtt gggcttttcc tccggtcttt
    12841 tcccttgaaa agggggtaga gatggctgaa ggggcggaag atgtgagaca aagtttgcag
    12901 attctgttta gtactgagcc gggggaacgt cttatgcgtg aaaattatgg ctgcggatta
    12961 aatgatttta tgtttgaaaa tatccgcaat gaacttattg ctgaaattga atcccatatc
    13021 catgacaacg tattacgata tgaaccccgg gctgatatga ctgatattca ggttcgtcaa
    13081 tcccctggca tggggaatac tttgcaagtg caggtcatgt atcgcctgag agggagtgat
    13141 atcaatcaac aaatccaggg agtacttgca ctgagtgaag gccgggtgac ggaggtagta
    13201 tgagtgaagc gattgtggtg gatggtgacg tgttacagtt tgatcccaac tttggcaatc
    13261 ggcaggtgac ggttcccagc ccaggaaaaa ttagcggcac aggacatgcg caggtaagtg
    13321 gaaaaaaagt gtgtattctg ggggatgaga aacaggtcag ggtttctgca acctatatta
    13381 caacaacaca tactacgccg ggaacaggaa ccattactat cagtgctctg gatgctggcc
    13441 agcaggccct tcagtgtacc agtggggcgg ctttaattat caaggggcag caatttacgg
    13501 cgatgtttac gcctgaattg ccagccatga ataatacagt gactccgcca caaccggatg
    13561 ttacgacacc ttcatcagga aaaggacgtt ttatcactca acaaaatttt gctaccgtaa
    13621 atggtggttc aggtggtggt ggtagcggtg gtggcggttc tggcggtggc ggtagtggcg
    13681 gttctggcgg tggcggtagt ggcggaggtg gaagcggagg cggtggaagt ggcggtgcac
    13741 cgaaaaagaa acgcaaagtt ggtattcatg gtgttccggc agcaatggat aagaaatact
    13801 caataggctt agatatcggc acaaatagcg tcggatgggc ggtgatcact gatgaatata
    13861 aggttccgtc taaaaagttc aaggttctgg gaaatacaga ccgccacagt atcaaaaaaa
    13921 atcttatagg ggctctttta tttgacagtg gagagacagc ggaagcgact cgtctcaaac
    13981 ggacagctcg tagaaggtat acacgtcgga agaatcgtat ttgttatcta caggagattt
    14041 tttcaaatga gatggcgaaa gtagatgata gtttctttca tcgacttgaa gagtcttttt
    14101 tggtggaaga agacaagaag catgaacgtc atcctatttt tggaaatata gtagatgaag
    14161 ttgcttatca tgagaaatat ccaactatct atcatctgcg aaaaaaattg gtagattcta
    14221 ctgataaagc ggatttgcgc ttaatctatt tggccttagc gcatatgatt aagtttcgtg
    14281 gtcatttttt gattgaggga gatttaaatc ctgataatag tgatgtggac aaactattta
    14341 tccagttggt acaaacctac aatcaattat ttgaagaaaa ccctattaac gcaagtggag
    14401 tagatgctaa agcgattctt tctgcacgat tgagtaaatc aagacgatta gaaaatctca
    14461 ttgctcagct ccccggtgag aagaaaaatg gcttatttgg gaatctcatt gctttgtcat
    14521 tgggtttgac ccctaatttt aaatcaaatt ttgatttggc agaagatgct aaattacagc
    14581 tttcaaaaga tacttacgat gatgatttag ataatttatt ggcgcaaatt ggagatcaat
    14641 atgctgattt gtttttggca gctaagaatt tatcagatgc tattttactt tcagatatcc
    14701 taagagtaaa tactgaaata actaaggctc ccctatcagc ttcaatgatt aaacgctacg
    14761 atgaacatca tcaagacttg actcttttaa aagctttagt tcgacaacaa cttccagaaa
    14821 agtataaaga aatctttttt gatcaatcaa aaaacggata tgcaggttat attgatgggg
    14881 gagctagcca agaagaattt tataaattta tcaaaccaat tttagaaaaa atggatggta
    14941 ctgaggaatt attggtgaaa ctaaatcgtg aagatttgct gcgcaagcaa cggacctttg
    15001 acaacggctc tattccccat caaattcact tgggtgagct gcatgctatt ttgagaagac
    15061 aagaagactt ttatccattt ttaaaagaca atcgtgagaa gattgaaaaa atcttgactt
    15121 ttcgaattcc ttattatgtt ggtccattgg cgcgtggcaa tagtcgtttt gcatggatga
    15181 ctcggaagtc tgaagaaaca attaccccat ggaattttga agaagttgtc gataaaggtg
    15241 cttcagctca atcatttatt gaacgcatga caaactttga taaaaatctt ccaaatgaaa
    15301 aagtactacc aaaacatagt ttgctttatg agtattttac ggtttataac gaattgacaa
    15361 aggtcaaata tgttactgaa ggaatgcgaa aaccagcatt tctttcaggt gaacagaaga
    15421 aagccattgt tgatttactc ttcaaaacaa atcgaaaagt aaccgttaag caattaaaag
    15481 aagattattt caaaaaaata gaatgttttg atagtgttga aatttcagga gttgaagata
    15541 gatttaatgc ttcattaggt acctaccatg atttgctaaa aattattaaa gataaagatt
    15601 ttttggataa tgaagaaaat gaagatatct tagaggatat tgttttaaca ttgaccttat
    15661 ttgaagatag ggagatgatt gaggaaagac ttaaaacata tgctcacctc tttgatgata
    15721 aggtgatgaa acagcttaaa cgtcgccgtt atactggttg gggacgtttg tctcgaaaat
    15781 tgattaatgg tattagggat aagcaatctg gcaaaacaat attagatttt ttgaaatcag
    15841 atggttttgc caatcgcaat tttatgcagc tgatccatga tgatagtttg acatttaaag
    15901 aagacattca aaaagcacaa gtgtctggac aaggcgatag tttacatgaa catattgcaa
    15961 atttagctgg tagccctgct attaaaaaag gtattttaca gactgtaaaa gttgttgatg
    16021 aattggtcaa agtaatgggg cggcataagc cagaaaatat cgttattgaa atggcacgtg
    16081 aaaatcagac aactcaaaag ggccagaaaa attcgcgaga gcgtatgaaa cgaatcgaag
    16141 aaggtatcaa agaattagga agtcagattc ttaaagagca tcctgttgaa aatactcaat
    16201 tgcaaaatga aaagctctat ctctattatc tccaaaatgg aagagacatg tatgtggacc
    16261 aagaattaga tattaatcgt ttaagtgatt atgatgtcga tcacattgtt ccacaaagtt
    16321 tccttaaaga cgattcaata gacaataagg tcttaacgcg ttctgataaa aatcgtggta
    16381 aatcggataa cgttccaagt gaagaagtag tcaaaaagat gaaaaactat tggagacaac
    16441 ttctaaacgc caagttaatc actcaacgta agtttgataa tttaacgaaa gctgaacgtg
    16501 gaggtttgag tgaacttgat aaagctggtt ttatcaaacg ccaattggtt gaaactcgcc
    16561 aaatcactaa gcatgtggca caaattttgg atagtcgcat gaatactaaa tacgatgaaa
    16621 atgataaact tattcgagag gttaaagtga ttaccttaaa atctaaatta gtttctgact
    16681 tccgaaaaga tttccaattc tataaagtac gtgagattaa caattaccat catgcccatg
    16741 atgcgtatct aaatgccgtc gttggaactg ctttgattaa gaaatatcca aaacttgaat
    16801 cggagtttgt ctatggtgat tataaagttt atgatgttcg taaaatgatt gctaagtctg
    16861 agcaagaaat aggcaaagca accgcaaaat atttctttta ctctaatatc atgaacttct
    16921 tcaaaacaga aattacactt gcaaatggag agattcgcaa acgccctcta atcgaaacta
    16981 atggggaaac tggagaaatt gtctgggata aagggcgaga ttttgccaca gtgcgcaaag
    17041 tattgtccat gccccaagtc aatattgtca agaaaacaga agtacagaca ggcggattct
    17101 ccaaggagtc aattttacca aaaagaaatt cggacaagct tattgctcgt aaaaaagact
    17161 gggatccaaa aaaatatggt ggttttgata gtccaacggt agcttattca gtcctagtgg
    17221 ttgctaaggt ggaaaaaggg aaatcgaaga agttaaaatc cgttaaagag ttactaggga
    17281 tcacaattat ggaaagaagt tcctttgaaa aaaatccgat tgacttttta gaagctaaag
    17341 gatataagga agttaaaaaa gacttaatca ttaaactacc taaatatagt ctttttgagt
    17401 tagaaaacgg tcgtaaacgg atgctggcta gtgccggaga attacaaaaa ggaaatgagc
    17461 tggctctgcc aagcaaatat gtgaattttt tatatttagc tagtcattat gaaaagttga
    17521 agggtagtcc agaagataac gaacaaaaac aattgtttgt ggagcagcat aagcattatt
    17581 tagatgagat tattgagcaa atcagtgaat tttctaagcg tgttatttta gcagatgcca
    17641 atttagataa agttcttagt gcatataaca aacatagaga caaaccaata cgtgaacaag
    17701 cagaaaatat tattcattta tttacgttga cgaatcttgg agctcccgct gcttttaaat
    17761 attttgatac aacaattgat cgtaaacgat atacgtctac aaaagaagtt ttagatgcca
    17821 ctcttatcca tcaatccatc actggtcttt atgaaacacg cattgatttg agtcagctag
    17881 gaggtgacaa acgtccggca gcaaccaaaa aggcaggtca ggccaaaaag aaatagagta
    17941 ttgactgaat taaatagaat aacgaaggtg taaataatta tttatttgct gacgaatcgc
    18001 tgtgacaaat aaacacaggt gatgttatgg aattaaatga gttaactaac aaattgtcaa
    18061 atttggtgcc aatgaccgat tttaaattag ataatcgagc cagtttgcaa ttgcttaaat
    18121 atattgaagc gtatacgaag ataataccct ttaattctgg cgataaatat tggaatgact
    18181 ttttctttat gtcaggaaat acgccagaga aacttgcaaa attatatcag aaagaaatag
    18241 aacccaatgg ggagttatta cctcagcagg cttttttgtt ggcggttttg cgtttattgg
    18301 aaacaccaat atccttatta aatgtattac ctgctgctca tcgtgagctc tattatcggg
    18361 agcttttagg cttgtcttcc catgcggcac agcctgatca ggttgcttta tctatggaac
    18421 tgaattcgac agtgatggaa cagctgctcc ctgaaggaac cctgtttgag gctggtcagg
    18481 atgaacaagg caatgcattg caatatgccc tggatgccag tttgctggct aatcgtggat
    18541 atatcagtga cttgcgctgg ttacggaatg acggggaaaa gcaatgggtt acttctgctc
    18601 catgggattt acaggcacag gtgtcactgc cgtctgatgg gatacgatta tttggtaaga
    18661 caaatagtga tcagcaggta tttggtgggg tgttgataac gtcatcactt ctggcgatgg
    18721 aagcggggat aaggaagatc attgttactt ttgagcagga gatgaacacc caagaactgg
    18781 tggcacaggt cagcagtgga aatcaatggc taacattgac gtctgaggta aataagaaag
    18841 aggtcacact gacactgtca gacaaagaac cggcaatcag tgcgccagag gatctggata
    18901 atctcttttt cacgcaaccg gtactcaggc tacagggaaa ggatagtcag gcactgccgg
    18961 aggtgacggg tatcagcgtt tcggaaaagg atgatactaa ggatacctct tttgagatgt
    19021 atcacttaac accatttggt tatagcagtg atatagagcc attggaggaa aatccagcgt
    19081 tatatttagg ctttactgat gtaaagccag ggcaaacact ggcgctgtat tggaaattaa
    19141 aatccccgca gcaaccaacc gtttcctggt attacctgga tcaacataat caatgggctg
    19201 aattggattc atgggtcagt gatggaaccc agaatctgta tcaggatggt acttggcacg
    19261 ttgagttgcc tgtggatgca tccaatcagg cagagcagat gccagttgga cgctattggt
    19321 tgcgggcagt ggtggaggta cccgctcatg agggggcgtt ggggaaggct ccttggctat
    19381 atggtctaat ctataacgcc atgacggcaa ccttggttaa tgtagatagc atcagtgaca
    19441 gccatttctt aacccctttg cctgccagca gcatacagcg gcccgttgaa cccatcattg
    19501 tgttggcatc ggtcaaccag ccttgggcat catggggtgg acgtatacct gaatcctaca
    19561 gtgccttttt tgaacggata gctcaaaacc tgtctcatcg aaaccggtcc ttaacctggg
    19621 gaaatatggt gacattactc aaagagcgtt atgtcagcat ctttgatgtt aagtatccag
    19681 gtaatgatga actcaccaga gtgccagcat tggagcagca gcaactaaca gtgattccag
    19741 caaaccggta caacgatagc gatgattctc tgcgtccggt actgaatcct gctcgtctgc
    19801 aagagatggc tgattggttg cagcagaaag actctccctg ggcctctatt gaggtcagga
    19861 atccagaata cttggatgtg aaaatccatt acgaggtgat ttttaaacct gatgtgaacg
    19921 aagattttgg ctatcgccag ctacagcagc aactgtgtga ggtgtatatg ccttggagca
    19981 tagatgagca gcggcccgtt gtattgaata acagcattaa ttatttccag ttgttagcca
    20041 ctattcaaca gcaaccgctg gttgagcgag tcactcgtct gacactacat cgggctgatt
    20101 cttctgatga gagtgatggt acagcatctg tggaagccaa agataatgaa gtgcttattt
    20161 tagtctggga agaggacgat aatctgcaat accgaggaaa tgactatgag taatcaggat
    20221 gcactgtttc atagcgttaa agacgatatt cactttgata ccttgctgga acaagctcat
    20281 caggtgattg aaaaacaggc tgaaaaactg tggagtgata cggcagagca tgatccgggt
    20341 atcacatttt tgcagggaat cagttacggt gtgtcagatt tggcttaccg acatacatta
    20401 cccctgaaag atttactgac tccggcgccg gatgagcagc agcaagaggg aatttttcct
    20461 gccgaatttg gcccgcataa tacactgact tgtgggccgg tgacagcgga tgattatcgc
    20521 aaggcattgt tagatctaca cagcagcgac agcctggatg gtactcagca ggatgagggg
    20581 gattttctgt tccggagtgt gcaactggtg cgtgaaccgg aaaaacagcg ttatacctat
    20641 tggtatgatg caaccaagag ggaatatagc tttgtcaaca gtgaaggggc taaagagttt
    20701 accttgcggg ggaattactg gttgtatctg gaaccaaccc gttggactca gggtaatatt
    20761 gccgctgcta ccagacaact gacagaattt ttgactaaaa atcgcaatat tggtgaatct
    20821 gtcagcaaca ttatctggct acaaccggtt gatctgccac tgttgctgga tgttgaactg
    20881 gatgatgatg taggtgcaca ggatgtcccc ggtatttttg cggcggtgta tagcaccgca
    20941 gagcagtatc tgatgcctgg agcacagcgt taccgtacgg aagtactgca aaatgctggg
    21001 atgagcaatg atcaaatctt cgaaggtcca ttattggaac atggctggat accagagctg
    21061 ccggcagccc gtgattatac tcaaaggctc actctcaatc ttagccggtt ggtaaatagt
    21121 ctgcttgaga ttgagggcat taaacatgtg aatcgtcttc gtctggatga tagcttcgat
    21181 aaaactgcta ttgaacccgt taagggggat acctggtcgt ggtcgatcaa agagggctat
    21241 tatccacgtc tttggggaga agacccactt aaccaattgg cgcaacaaaa tggcccgctt
    21301 agggtgatag ccaaaggagg gattagcgtc agtgtgagta aagagcaaat ccaggccagt
    21361 ttacccagtc aatcactgat tcaaaatgag ccggtaatat tggcttacgg ccagcaccgt
    21421 gacgttggca gctattatcc cgtcagtgat actttgccgc cttgctatgg actacaacat
    21481 tctttgtctg aaagtgaaca cttattgcca cttcatcaat ttatgttgcc atttgaacaa
    21541 ttattggcct gtggttgtca acagatagcc atgctcccgc ggttactggc ttttcagcgc
    21601 gaaggttatg aggtttgggg tgatcagtgg ccctttaagt caggctcagt gaatgatgac
    21661 gcccatcaag attatgcccc tgcattaaag gatttgttag gacagattgc gctggatagt
    21721 gatcatgaat tggatattat taattacttg ctgggttact ttggcacaca gcgggcaccg
    21781 cgtaccttta cgacacaact cgatgatttt cgtgcggtcc aacagggtta tctggcccag
    21841 caaccgacat tgacttacca ccgctccaat attcgtatcg atcaggtatc gtcgctacaa
    21901 aaacgtattg ctgctcgcat ggggctgggc ggtgagttgt ttaaacctca accggatctg
    21961 agccaactgc ctttttattt gattgaacat cgagcgttgc tgccagtcaa acccaatagt
    22021 cagtttgata aggaacagaa accagcctcg gtgacagagg aggggggcag ccaaacaggt
    22081 caacattatg tggtcattga acagaagggc attgatggca agctgacaca ggggcaagtg
    22141 atcaatttaa ttctgtatga aggagagcag ggagaaaccc aatttacgat acgcggtcag
    22201 atggtattca aaaccgaggg ggataagttt tggttggatg tgaataatag tgcgcaactg
    22261 gaatataatc tggcgcgggt aatgacagca gccaaggcga gtaaactctt ttggcaaaac
    22321 agcccggtat ggatggagga tatgggctat cgtctggcct atgctagtga ccaatcctca
    22381 ttgcctgtga atcaacggcg cttgacccgc acagtgcaaa ctccattccc gccgatggtt
    22441 gttgtaggta gcgaaatcac cctgttaaag caggtgggga tagtcaattt aaaaaaagcg
    22501 gagtcagaaa aactttatgc aaaagttgtt agctttgatc gcattgaagg gaccttgatt
    22561 attgagcgtt tgggtaattc cactctggct tttcctacct cggaagaggc gtggcggtat
    22621 agttggtatt tttcggggga gaaatatgaa aggactgacc gcttttcatt tgtgattagc
    22681 gtagtagtga acagtgactt aattaaattg cccggtgttg atccctataa attggaagaa
    22741 tgggtgaaag aaacgattct taccgaattt ccagctcata tttctatgat tatccattgg
    22801 atggatcggg aagccttttt aaatttcgcc aatacctatc agcgttggca aaataatggt
    22861 acgccactgg gggatgcggc ttattccatt ctagaaagtt tgacacttgg taaattgcca
    22921 tctgccttaa aaggtgttgg cacaatgcgt attgccacat ctagtcaaag agaagaagtg
    22981 gtgggtagta atggtgatca atggaataca gatggaataa cccagaatga attattctat
    23041 gttcctaaag agagctagga aaaataaata tctgccacta atgatgttga attaaatatg
    23101 ttttctggag ttaatcatga acgaaactcg ttataatgca actgtacaag aacaacaaac
    23161 attatctaat ccaaaagctg ttggacctga catcgataaa ttaaaggata aatttaaaga
    23221 gggcagtatt cccctgcaaa ccgatttcaa tgagttaatt gatattgccg atattggacg
    23281 taaagcctgt ggtcaagcgc cacaacaaaa tggcccagga gaaggattga aattggctga
    23341 tgacggtacg cttaatttaa aaataggcac tttttccaat aaagactttt ctccattaat
    23401 attaaaagat gatgttttat ctgtagatct tggtagtggt ctgactaatg aaaccaatgg
    23461 aatctgtgtc ggtcagggcg atggtattac agttaacact agcaatgtag ctgtaaaaca
    23521 aggtaacgga attagcgtta ctagtagtgg tggtgttgcc gttaaagtta gtgctaataa
    23581 gggacttagc gttgatagta gtggtgttgc agttaaagtt aatactgata agggaattag
    23641 cgttgatggt aatggtgttg cagttaaagt taatactagt aaaggaatta gcgttgataa
    23701 tacaggtgtt gcagttatag ctaatgctag taagggaatt agcgttgatg gtagtggtgt
    23761 tgcagttata gctaatacta gtaaaggaat tagcgttgat ggtagtggtg ttgcagttat
    23821 agctaatact agtaaaggaa ttagcgttga taatacaggt gttgcagtta tagctaatgc
    23881 tagtaaggga attagcgttg atggtagtgg tgttgcagtt atagctaata ctagtaaagg
    23941 aattagcgtt gatggtagtg gtgttgcagt tatagctaat actagtaaag gaattagcgt
    24001 tgatagtagt ggtgttgcag ttaaagttaa agctaatggc ggaattaaag tagatgctaa
    24061 tggtgttgca attgatccta ataatgtact ccccaaggga gtgattgtaa tgttctctgg
    24121 cagtactgca ccaactggtt gggcgttatg tgatggcaat aatggtacac caaatttaat
    24181 cgatcgattt attttaggtg ggaaagggac tgatattaat ggagtgagta ctaatacagc
    24241 ttcaggtact aaaaatagta agttattcga tttcagttct gatgaagcta cattaactat
    24301 tgatggtaaa acactgggga gaggtggctc tggtggtggc ggttctggcg gccacgatca
    24361 tgatattaag ataacgggca caggaaaaca ttctcacaaa aacaaagtaa cagtccctta
    24421 ttatattctg gctttcatca taaagcttta atatatatga aaaattgaaa atataaatta
    24481 tccattaata ataaagagga tattagcatg acttcggagc caaatctgtt aaaccggatt
    24541 acaattacta ttgaagctaa taatcaacaa gtagctagaa aagtattgca tggctccttg
    24601 cttaatcaag ctaatataaa taaattattt aattcatact ttaatgaata tgaaattaat
    24661 aggggtgttt atttagaaac attaatcctg aatcttggta cgataaattt ccatgatttt
    24721 aattcattgt ttcctactct cctaaaagct gcattgaata aagaattcag tcaatatcag
    24781 ataaacaacc atagggaaga aatgctattt aatgagacaa tatcaaatca agctactgat
    24841 aagtcttaca tatttggcga taacaaatta attgatgcag agaatttcat tcacttttta
    24901 tatcaaaagc attccacatt aaatctagta gaagcaatgg gaaataatgg tattgaaaaa
    24961 ttaacaaatc agttaacaca aatagaaaat aaatttgcgt tattattggc aaaaagttgt
    25021 ttgtctgagg aaggcttaaa acgactcttg gctatcaaac aacccgattt attaatcgct
    25081 atcaatcgca gattatctga aagaataaat agaccacaat atcaggagaa gcttgtttcc
    25141 tgcggacaac tgatatttag tgctctggga tatatacaac agtacaatat acaggaaatt
    25201 cctaaaccgg atgaaaaagt tattgcacgc ataacaactg aacttaataa taatggtttg
    25261 cttaatacaa tacctattat tacactattt cgtcagagtg ggattaacga ttcatcacta
    25321 aatgattggc taaagaaaat ctggcaggtg agatcaattt cacagttatg cagaaagtat
    25381 ctttctgcta aggaatacca atatctgtca gaacattttg tttcaaagag cgtcgataaa
    25441 aatagatatg atgaagagcc cgtaaatcag agcatattat caaggttgaa taataattcc
    25501 attaaagaag gaaataatca cagtcaactc tgtactctca gtagactata ttctgaaccc
    25561 gttgtattac ctgaacaaac cattctacgt caggttagta atacagtaga tcagagcata
    25621 ttatcaaggt tgaataatgc ctccattaaa gaaggaaata accaaagtca acttcgcact
    25681 ctcagtagac tatattctga gcccgttgca ttacctgaac aaaccattcc acgtcaggtt
    25741 agtaatacag gtatattaat tctatggcca atgctaccta cactatttaa ccagcttggt
    25801 ctacttgaga aaaagaaatt tatccatcgt caggcccagt ttaatgccgt tgattttctt
    25861 gattacctga tttggggaac cgaagatgtg aaagtggaac gaaaggtttt gaataatgtt
    25921 ctatgtgggt taatggctga tgaaattact gaaccaatgc ctattgaacc agaaaaacaa
    25981 tggataataa ttcaatggct ggacgctatt atctcccaac tttctggctg gaaaaagtta
    26041 agtcgtaatg acgtccgtca attatttcta caacgaccag gagaattact gatcaatgaa
    26101 caggaaatta aaatcacaat acagcaacaa ccatttgatg ctctgttaac tgattggccg
    26161 tggccaatga atatggcttg ttttagctgg ttgagtcaac cattaaccat tacgtggtta
    26221 taaccattga ccacaatgac ttagtctgag taaaaaatat gaatatatcg cctgtttttt
    26281 atgattcatt gaatcaggat aacgaccgtg atctatcgtt tttatttagc gaactggaac
    26341 gaatagatct cgctcttcaa caccattttt attgtgtaga aagtcagcga agtgagctcc
    26401 tggatgagtt tctgctcact gaggcggaag tggtgaccag gctggataag ccacttggta
    26461 aacctcattg gataaatgat gattatctgg cgatatcgca aaagggcaat gtaagcctaa
    26521 tggcagcgtc cagattaatg gatctgatcg aacgctttga actgactgat tttgagcgcg
    26581 atgttttact attaggctta ttgccccatt ttgatagccg ctattatcga ctgttttcgc
    26641 tgattcaagg gggacaacag ggtcgattac cttcttttgc gctggcattg gaactgtttt
    26701 gccactcggc gctggagaaa caggtacagc aagcgagttt tctgcaccgg gcacctttga
    26761 tgggttgcca gctattatcc atcgatacta gtcaaaaaac gctggcctgg ctccagactc
    26821 cctttattac tgacagcggg gtatatcact ttttactggg gcatcactac attatgccgg
    26881 ctttagaaca ttgtgctgag tggttaacac cgacagggat tggctgttat cctgaaggat
    26941 taaaacaagt actgggtaac gtattgttat ctgacaacga taatattaga ccgattgtct
    27001 tattacgggg aatggccggc agtgccagag cttataccat tactaatatg atggcttcag
    27061 aagggaagca aacactgctg gtagatatat ccaaacttgc tgatagcgat gaaaaaaaca
    27121 ttattcttca gataaagcat attttgcggg aaacccgcat gcatggagca tgtttattat
    27181 tacggaattt ttgcttgtta gtggaacaga ataaacaact attggactcc ctgtcagagt
    27241 tattgaatca acctgaatta agaattgttt gcctgattga gccttattcc ccattggtat
    27301 ggctgaaaaa gataccggta ttactgattg agatgccact tttaacgcct gcggaaaaag
    27361 ccagattgtt aattgccagc ttaccggata attgttccga ggatattgat acgataactt
    27421 taagccagcg ttacactttt aacccagaaa ccctgccatt gattttgcaa gaggcccagc
    27481 tttatcaaca gcagcgagat ccgctggata tcttgcagca atgcgatata cgccaggcat
    27541 taaatttgcg tgctcaacaa aatttcggtc aattggcaca gcggattatt cctaagcgct
    27601 cattaaagga tttattggta tccgatgaga ttgctcagca gttacgggaa atactcatag
    27661 caattaagta tcgggaacag gttctggcgg gagggtttaa agataaaatt gcctatggca
    27721 ctggtatcag cgccctgttt tatggtgatt caggcactgg aaaaaccatg gcagcagaag
    27781 tgattgctga ccacattggc gttgacttaa taaaagtgga tttatctaca gtagtgaata
    27841 aatacatcgg tgaaacagaa aaaaacttat cccgtatttt cgatttggcg gaacaggatg
    27901 caggggtatt attctttgat gaagctgacg cactgtttgg taaacgcagt gaaactaaag
    27961 attcccagga cagacatgcc aatattgaag tttcttactt attacagcgc ctggagaatt
    28021 acccgggtct ggtcatttta tccaccaata atcgtggtca tttagacagt gcttttaatc
    28081 gtcgttttac tttcattacc cgttttactt acccggatga aaaaatccgt aaaaaaatgt
    28141 ggcaggaaat ttggcctaga aatataaaaa tatcggaaga tatcgatttt aacgaattag
    28201 ctcaacgaac aagcgtgact ggcgcgaata tccgcaatat tgctttattg tcttcattct
    28261 ttgcttcaga gcaggggaat gatgaagtca gtaatgaaaa tattgaaatt gcattgaagc
    28321 gtgaattagc taaagtcgga cgattaacat tttaaaagtt atcacaatga aagtattgaa
    28381 atattaaata aatttattac caaaaagtta tcacgatata atttaagaga ggttttttat
    28441 gttaaacacg caaactatta ttgatgtcaa taaggcaatg gatgccatgc tgcgcgcata
    28501 tctgaatcaa gatattgcca ttcgttttga tctacctgaa ttggatacta tgcaatctga
    28561 tgcgatggta agtatctttc tttatgacat tcatgaagat ttacagcttc gctcggcaga
    28621 atcaagaggg tttgatgttt atgccgggag gttattgcct ggttgggtaa atattaaatg
    28681 taactatctg attacctatt gggaagcttc taagccagcg actgatgcca gcagtccgga
    28741 tagccaacct gataaccagg caatacaagt gatgtcacaa gtattaaatg ccttgattaa
    28801 taatcgtcaa ttggcaggta ttcctggtgc ttatactcag gttgtaccgc ctaaagagag
    28861 tttaaatagc ctggggaatt tctggcaatc actgggtaat cgcccacggc tttctctcaa
    28921 ttattcagtg acagtacctg ttagcctaaa cgatggtcag gatagcgcga ctccggttac
    28981 cgcggtttct tctacagtgg aacaaacggc atcgctcagt caagaagtgg ttagtcatgc
    29041 tttacgcgaa ttactcatta cggaattagg aggaggagag gataaccggt tggtactgag
    29101 taaagttgaa ttatccgcag tgaaagagac gatgactcaa gacagtccgg ctcagatgat
    29161 tatattgttg tctgtttcag gcattacacg acaggaatat ttgaaggaaa ttgataatat
    29221 ctttgatcgt tgggtaaata atgctgaagt tattaccact attgatgatt gtgggattag
    29281 aattgaaagt ataacgaaag ataatcttgt aggaatttaa atttttttaa ttaaataaag
    29341 gaattaatat tgatttgata gagcatgtaa tcaataactt ctatgaaaat gttgaaaaac
    29401 aatacacgga aacacttaac ttacaattaa attgttaaag
//
