LOCUS       sequence-484640-       30006 bp    DNA     circular UNA 26-AUG-2025
DEFINITION  PVC structural operon - tail fiber (Pvc13) retargeted with Ad5 knob 
            - spike tip (Pvc10) loaded with Cas9 on CTD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 30006)
  TITLE     Feng Zhang Lab - PVC plasmids
REFERENCE   2  (bases 1 to 30006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 26, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAWP78-PVCpnf_pvc13-Ad5_pvc10-Cas9
FEATURES             Location/Qualifiers
     source          1..30006
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(339..1154)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(339..1154)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1062..1081
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1146..1165
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             complement(1458..2606)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(2878..3249)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            3282..3391
                     /note="incP origin of transfer"
     rep_origin      3544..4175
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      4484..5072
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4973..4992
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             13717..13737
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             13762..17865
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             13762..17865
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 gagatgatga taagaatggt cagtcaccag ataaaatgat ctctggtcaa ttttaggaaa
       61 gctgaaaaaa cctgaccaaa tgcgtcgtaa attctatgct tagaaagact cggtgccagt
      121 gtaataccta agatatggtt tttatttcca tatctgttcg aaaacttaga aataacaagt
      181 tcaacaataa ccagggactt taaatatcgg atccttgtcg ggaagatgcg tgatctgatc
      241 cttcaactca gcaaaagttc gatttattca acaaagccgc cgtcccgtca agtcagcgta
      301 atgctctgcc agtgttacaa ccaattaacc aattctgatt agaaaaactc atcgagcatc
      361 aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt
      421 ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat
      481 cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa
      541 ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa
      601 agcttatgca tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa
      661 tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg
      721 cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact
      781 gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct
      841 gttttcccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc
      901 ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta
      961 acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc
     1021 ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac
     1081 ccatataaat cagcatccat gttggaattt aatcgcggcc tcgagcaaga cgtttcccgt
     1141 tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt
     1201 catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga cacaacgtgg
     1261 ctttcgactt ccggcaagct atacgcgccc tagaattgtc aattttaatc ctctgtttat
     1321 cggcagttcg tagagcgcgc cgtgcgtccc gagcgatact gagcgaagca agtgcgtcga
     1381 gcagtgcccg cttgttcctg aaatgccagt aaagcgctgg ctgctgaacc cccagccgga
     1441 actgacccca caaggcccta gcgtttgcaa tgcaccaggt catcattgac ccaggcgtgt
     1501 tccaccaggc cgctgcctcg caactcttcg caggcttcgc cgacctgctc gcgccacttc
     1561 ttcacgcggg tggaatccga tccgcacatg aggcggaagg tttccagctt gagcgggtac
     1621 ggctcccggt gcgagctgaa atagtcgaac atccgtcggg ccgtcggcga cagcttgcgg
     1681 tacttctccc atatgaattt cgtgtagtgg tcgccagcaa acagcacgac gatttcctcg
     1741 tcgatcagga cctggcaacg ggacgttttc ttgccacggt ccaggacgcg gaagcggtgc
     1801 agcagcgaca ccgattccag gtgcccaacg cggtcggacg tgaagcccat cgccgtcgcc
     1861 tgtaggcgcg acaggcattc ctcggccttc gtgtaatacc ggccattgat cgaccagccc
     1921 aggtcctggc aaagctcgta gaacgtgaag gtgatcggct cgccgatagg ggtgcgcttc
     1981 gcgtactcca acacctgctg ccacaccagt tcgtcatcgt cggcccgcag ctcgacgccg
     2041 gtgtaggtga tcttcacgtc cttgttgacg tggaaaatga ccttgttttg cagcgcctcg
     2101 cgcgggattt tcttgttgcg cgtggtgaac agggcagagc gggccgtgtc gtttggcatc
     2161 gctcgcatcg tgtccggcca cggcgcaata tcgaacaagg aaagctgcat ttccttgatc
     2221 tgctgcttcg tgtgtttcag caacgcggcc tgcttggcct cgctgacctg ttttgccagg
     2281 tcctcgccgg cggtttttcg cttcttggtc gtcatagttc ctcgcgtgtc gatggtcatc
     2341 gacttcgcca aacctgccgc ctcctgttcg agacgacgcg aacgctccac ggcggccgat
     2401 ggcgcgggca gggcaggggg agccagttgc acgctgtcgc gctcgatctt ggccgtagct
     2461 tgctggacca tcgagccgac ggactggaag gtttcgcggg gcgcacgcat gacggtgcgg
     2521 cttgcgatgg tttcggcatc ctcggcggaa aaccccgcgt cgatcagttc ttgcctgtat
     2581 gccttccggt caaacgtccg attcattcac cctccttgcg ggattgcccc gactcacgcc
     2641 ggggcaatgt gcccttattc ctgatttgac ccgcctggtg ccttggtgtc cagataatcc
     2701 accttatcgg caatgaagtc ggtcccgtag accgtctggc cgtccttctc gtacttggta
     2761 ttccgaatct tgccctgcac gaataccagc tccgcgaagt cgctcttctt gatggagcgc
     2821 atggggacgt gcttggcaat cacgcgcacc ccccggccgt tttagcggct aaaaaagtca
     2881 tggctctgcc ctcgggcgga ccacgcccat catgaccttg ccaagctcgt cctgcttctc
     2941 ttcgatcttc gccagcaggg cgaggatcgt ggcatcaccg aaccgcgccg tgcgcgggtc
     3001 gtcggtgagc cagagtttca gcaggccgcc caggcggccc aggtcgccat tgatgcgggc
     3061 cagctcgcgg acgtgctcat agtccacgac gcccgtgatt ttgtagccct ggccgacggc
     3121 cagcaggtag gcctacaggc tcatgccggc cgccgccgcc ttttcctcaa tcgctcttcg
     3181 ttcgtctgga aggcagtaca ccttgatagg tgggctgccc ttcctggttg gcttggtttc
     3241 atcagccatc cgcttgccct catctgttac gccggcggta gccggccagc ctcgcagagc
     3301 aggattcccg ttgagcaccg ccaggtgcga ataagggaca gtgaagaagg aacacccgct
     3361 cgcgggtggg cctacttcac ctatcctgcc cggctgacgc cgttggatac accaaggaaa
     3421 gtctacacga accctttggc aaaatcctgt atatcgtgcg aaaaaggatg gatataccga
     3481 aaaaatcgct ataatgaccc cgaagcaggg ttatgcagcg gaaaagatcc gtcgaccctt
     3541 tccgacgctc accgggctgg ttgccctcgc cgctgggctg gcggccgtct atggccctgc
     3601 aaacgcgcca gaaacgccgt cgaagccgtg tgcgagacac cgcggccgcc ggcgttgtgg
     3661 atacctcgcg gaaaacttgg ccctcactga cagatgaggg gcggacgttg acacttgagg
     3721 ggccgactca cccggcgcgg cgttgacaga tgaggggcag gctcgatttc ggccggcgac
     3781 gtggagctgg ccagcctcgc aaatcggcga aaacgcctga ttttacgcga gtttcccaca
     3841 gacgatgtgg acaagcctgg ggataagtgc cctgcggtat tgacacttga ggggcgcgac
     3901 tactgacaga tgaggggcgc gatccttgac acttgagggg cagagtgctg acagatgagg
     3961 ggcgcaccta ttgacatttg aggggctgtc cacaggcaga aaatccagca tttgcaaggg
     4021 tttccgcccg tttttcggcc accgctaacc tgtcttttaa cctgctttta aaccaatatt
     4081 tataaacctt gtttttaacc agggctgcgc cctgtgcgcg tgaccgcgca cgccgaaggg
     4141 gggtgccccc ccttctcgaa ccctcccggc ccgctaacgc gggcctccca tccccccagg
     4201 ggctgcgccc ctcggccgcg aacggcctca ccccaaaaat ggcagccaag ctgacccgct
     4261 agggacgtga agtcgattcc ttcgatggtt agcaatcaaa gactcagagt gctgtctgga
     4321 gcgtgaatct aacggtacgt atctcgattg ctcggtcgct attcgcactc tgcgaaagtt
     4381 cgtaccgctc attcactagg ttgcgaatca tgaccaaaat cccttaacgt gagttttcgt
     4441 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     4501 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     4561 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     4621 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     4681 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     4741 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     4801 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     4861 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     4921 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     4981 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     5041 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     5101 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     5161 tggataaccg tattaccgcc tttgagtgag ctgtcgcgaa tgcatctaga tataattaca
     5221 tcttcatcat tcggtatttt aaatgacata tatcctcctt ccgaatagtt ttaatggtga
     5281 tgttcgattg gacagtgttt tccattatta aaattttaag tatcacatca tgtttaatga
     5341 aggattgatg tttattagaa tattcataat agctttgtct ttgcctctat gtaaatgaaa
     5401 ttgcataaaa agcaaattgc attattggca atgtacttat tctggatttt tcggcacgat
     5461 aaattattgg tgtaagtttt atatcatgag taaaaattcg gaatgatatt cctgtcattt
     5521 tgtggcggta gcgttactca agatattatc ctacatcata tgttgattat tgtaatcaat
     5581 cgagctattt tttgtctaaa aaggggcttt tatgtctaca agtacatctc aaattgcggt
     5641 tgaatatcct attcctgtct atcgctttat tgtttctgtc ggagatgaga aaattccatt
     5701 taatagtgtt tcaggattag atattagtta tgacaccatt gaataccgag atggtgttgg
     5761 taattggttc aaaatgccgg gtcagagtca gagcactaat atcaccttgc gtaaaggcgt
     5821 tttcccgggg aaaacagaac tgtttgattg gattaactct attcagctta atcaggtaga
     5881 gaaaaaggat attaccatca gtttaactaa tgatgcaggt accgaattat taatgacctg
     5941 gaatgtttct aatgcttttc ccacttcatt gacttcacct tcatttgatg ccaccagtaa
     6001 tgatattgca gtacaggaaa ttacgctgat ggcagatcgg gtgattatgc aggctgtttg
     6061 aagcattgat atttaatcat ctcatataag ggaactttta tgacaaccgt taccagttat
     6121 cctggcgttt atattgaaga attaaatagc ctggccttgt cagtttcaaa tagcgccaca
     6181 gcggttcctg tttttgctgt ggacgaacaa aaccaatata ttagtgaaga taatgcaatc
     6241 cgtattaatt cgtggatgga ttatcttaat ctgattggca attttaataa tgaagacaaa
     6301 ttagatgttt ctgtgcgtgc ttattttgcc aatggaggtg gatattgtta tctcgtcaaa
     6361 acaacgagtt tagaaaaaat tattccaacc ttggatgatg taaccttatt ggttgctgcg
     6421 ggcgaagata ttaaaacgac agtagatgtt ttatgtcagc caggaaaagg gttattcgca
     6481 gtctttgatg gccctgaaac agagttgact atcaacggtg cggaagaggc aaaacaagcc
     6541 tataccgcca caccattcgc tgcggtttat tatccttggt tgaaagcgga ttgggctaac
     6601 atagatattc cacccagtgc agtgatggcg ggagtttatg catcggtgga tttatcccgt
     6661 ggtgtatgga aagcgcctgc caatgttgcg ttgaaagggg gcctggaacc taaattttta
     6721 gtcacggatg aattgcaggg tgaatataac actggccgcg ctatcaatat gattcgtaat
     6781 ttcagtaaca caggtactac ggtttggggt gcaagaaccc tggaagataa agacaattgg
     6841 cgttatgttc cagtgcgacg cttgtttaat tctgtggagc gggatatcaa gcgtgccatg
     6901 agctttgcta tgttcgagcc taataatcag cctacttggg agcgggtacg ggcggcgatt
     6961 agcaactacc tttatagcct gtggcaacag gggggattag ctggcagcaa agaagaagac
     7021 gcttattttg tgcaaattgg taaaggtata acgatgacac aggagcagat tgatgcaggg
     7081 caaatgattg ttaaagtcgg tttggctgct gtacggcctg cggaatttat cattctccag
     7141 tttacgcaag atgtagaaca gcgttaatca tatgattatg aggagttatc atgtctgcta
     7201 ttctgaaagc gcctggcgtt tatattgaag aagacgcttc cctagcgttg tctgtcagta
     7261 acagcgcgac tgccgtgcct gtttttatcg gaaaatttac tccgacagtg gttgattcaa
     7321 tccaagtctg tacccgtatc agcaactggc ttgaattcac ttcctctttt tccctagctc
     7381 caacagttga gattgttgtc caatctaaca ctgaatctga atctgaatct gaaacttacc
     7441 actatattga gacaatcaat ctatctccag ctgtggaagc attgcgactc tattttcaaa
     7501 atggcggagg agcttgctat atctacccat taaatgatgc tgaagatgaa ttggttctgg
     7561 cggccatacc agaagtcatt gaacagaaag gtgatattac tctgttggtt tgcccggaac
     7621 tcgatctgga ttacaaaact aagatctatg gcgcagtgag ctcactgttg aatgataaca
     7681 aagtgggcta tttcctgatt gcggatagca atgatggaga atctgtgtca ggagtatgga
     7741 atagtgctaa ggccgccgcc tattatcccc agttggaaac taacctaaaa ttttccacgt
     7801 tgcctgggga taaggacatt cgtatcagcg gttatcagga tgatgatgaa acacataaac
     7861 cgaaaaactt ggatgagctc aggacaatca acgaggcgtt ggcacaggat attgatgcaa
     7921 gattgctcga ggagaaacaa cgtgctgtca tcattccgcc aagtgctgcc attgcgggca
     7981 tttattgcca aacggataat cgtcgcggtg tttggaaagc gccagccaac gttgcgctca
     8041 cagggatcgg gagtttgctt gataaggtag acgatgaacg gcagggagag atgaatgaca
     8101 agggaatcaa tgtcatccgt tcatttaccg accgtggttt tatggtctgg ggagcccgta
     8161 cttgtgtgga cgctgccaac atcagctggc gttatattcc tgttcgtcgc ctgttcaatt
     8221 ccgttgaacg agatatccgc caggcgctac gcgctgtgtt gtttgaaact aatagtcagc
     8281 ctacctgggt acgtgctaag gctgccgttg atcaatatct ttataccctt tggcagaaaa
     8341 atgcattgat gggtgctcgc ccggaagaag cttattttgt gcaaattggt caggatatca
     8401 ccatgtccga ggctgatatt aaacagggta agatgatcat gactgttggt ttggcagcag
     8461 tgcggccagc tgagttcatc attctgcaat ttacgcagga tgttgttcag taatctccat
     8521 gactaaacgc caggcactgt attgacagtg cctactctaa ccatcttgga ggaggtgatg
     8581 atgatggaga gactccaacc gggtgtgact ttaacagaaa gtataatcac gatgggtcag
     8641 caagagatac ccagtgctgt gccggtgttt attggttaca ccgttcgtta tccggaacaa
     8701 tcggaagcat cagtccgtat cgacagtttg gccgagtata ccagcctgtt tggtgacgac
     8761 catgtgatga tgtttgctgt caggcactat tttgataatg gcgggcaaca ggcatttgtt
     8821 ttacccctga aggacaatat gccatcagtg gagatgacca cagctgaagc ggaaaatctg
     8881 atagccgcat tgcgctctgc tacggttagc gaagccattg gtgggcatag tcagattaca
     8941 ctgattttgg taccggatat ggctcggctt aatgacagtg atattgatga ctcctcaacc
     9001 caggtaagcc tgtggtccca aggctgggag gcgctgctgc aattgagtca ggttaggccc
     9061 aacctctttg tgctgttaga tgcgccggat aatgttgaac aggcgcagaa gtgtatgaca
     9121 acgctatcgt cagattatcg tcaatggggg gcagcatatt ggcctcgtct ggaaactacc
     9181 tatcagaaag aaatatctgg caaggacaat gaatctcagg gaattttcca ggggactgtt
     9241 ctgtcaccca cagccgcggt cgcagcggta attcaacgca cggataacga cgcgggtgtt
     9301 tggaaagcac cggccaatat tgccttatcc caggttattc gacctgttaa atcttatctt
     9361 cagggaagtg tactgtttaa cagcagcggc acttcgctca atgtgatccg cagtttccca
     9421 ggtaagggca tacgggtatg gggatgccgc actctggaaa acacggataa tacgcagtgg
     9481 cgctatctgc aaacacgtcg gctggtttcc tatgtaacag cgcatttgac ccaattggct
     9541 cgcatgtatg tcttcgagcc aaataatgaa cttacctgga tgaagttaaa aggacaaagt
     9601 tacaactggt tacggcaatt atggttgcag ggtggcttgt atggttcaca ggaggatgag
     9661 gcatttaaca ttctgttagg cgtaaacgag acgatgactg aggatgatgt tcgtgcagga
     9721 aaaatgatca tgaaagttga gttggctgtg ttgtttcctg ccgaatttat tgagatcagt
     9781 ttggtgttta atacccaaac agaggcgctg tcttaagaag gaaaaagtac gatgaacgat
     9841 tattacacac ccgtggtatc ccatcgtttt atggcgagtt ttatttttaa ccgcattccc
     9901 gatccgctgg atattcgttt tcagcgtatc tctggcctta gtcgggaact acaggtgact
     9961 cagtacagtg agggaggaga aaatgcccgt aataactatt tagctgagaa aatccaacac
    10021 ggtacgttga ctttggaacg gggcgtgatg acagtctcgc cattgacctg gatgtttgat
    10081 cgggtattga gtggtgaaaa aatcgcttat gccgatgtgg tggtgatgct actgaatgaa
    10141 aattcactgc cattgtccag ttggacgttg agcaatgcgc tgccggtacg ctggcaaacc
    10201 agcgactttg acgctaacag caatgccata ttggtgaata cccttgaatt gcgttaccag
    10261 gatatgcgct ggcttggagt caaaatatga cagtagaaat cagagagtta cttatccagg
    10321 caaaggtagt gccatcaaca cgaccgactg aatcagaacg gcaaaaccat tctttgatac
    10381 aggaaagtct ggatgaggcg acttgggtgg aaacgataaa acgcgaagtg ttggccgcat
    10441 tacgcgatga ggaagggtgg cgtccatgag tctgattgaa cgtggtttag ctaagctgac
    10501 aattaatgct tataaggata gggaagggaa gatacgggca ggaacgttgc aggccatgta
    10561 taaccctgac tccttgcaac tggattacca aacggattat cagcaatccc aagcgattaa
    10621 tagcgaaaag caaagtagca tttatgtaca ggccaagccc gcagggttat cacttgaatt
    10681 aatttttgat gccacgatgc cgggtaacaa aacccccatt gaagagcagc tcatgcagct
    10741 caagcaactg tgcagtgtgg atgcaaccag taacgagacg cgattcctgc aagttaaatg
    10801 gggcaaaatg cgttgggaaa gtcggggtta ctttgctggc agggccaaga gtttgtctgt
    10861 gaattacact ttgtttgatc gtgatgcgac tcccttgagg gtacgggtaa tattggcatt
    10921 agtggctgat gaaagtctgg tgttgcagga gactgaacaa aatctgcaat ctccggcaaa
    10981 aatcgcatta cgcatacagg atggggtatc tctggctctg atggcagcca gtacggcatc
    11041 aacattgtca ggcggtgtgg attatctgac gctggcctgg caaaacggtc tggataatct
    11101 caatgggttc gttccgggtg aaatattgca ggccaccagg ggagacgaat catgagccac
    11161 caactgaaaa ttattgcaga tggtaaggca ctgtcacttt tggccgcggt agatgtggac
    11221 acctgttatc gggttaacag tataccttct gcgacattga aactgagcgt accggatagg
    11281 ccactctctt ctttcagtca gacggatgtt cagacagaac tggcccactg tcaggtaggg
    11341 aaaaccctgc gtctggaatt gattgatggt agcaaaaaat gggtgctgtt taatggtctt
    11401 attacccgta aggctctgag aattaagaat aagcaattat tgctcactct ggttgtcaag
    11461 catcggttgc aactgatggt ggatacccag cattcacagc tgtttaaaga caaaagcgaa
    11521 aaagcgatct taagcacgct attgaatcag accggaatca atgctcgctt cggaaagata
    11581 gcggcgttag atcaaaagca tgaacagatg gtgcaatttc gttgttcaga ctggcatttt
    11641 ctgttgtgcc gactgtcggc aaccggtgca tggttgttac ctgccataga agacgttcag
    11701 tttgttcaac ctgatgctct gaaatcaaac tcagcctata ccttgaagag caggggggat
    11761 gagaacaaag acatcgttgt caaggatgct tactggcagt ttgacaatca aatcaacccc
    11821 gctttgctgg aagtcagtgg ctgggatatc agtaagcagc aggtacaatc aggcggtcgc
    11881 tacggaaaaa tcgcgttggg taaggcggca ctctctcctg atggattggc atcccttaat
    11941 aaaacgggtt gggacatttg ttatagcagt ccgttaacaa cccaggaaag cggttatctg
    12001 gcacagggat tattgcttaa ccagcgcatt tctggggtga caggagaatt tttgctcaaa
    12061 ggagatgggc gttaccagtt gggagacaac attcagctga ctggatttgg ttcacagtta
    12121 gatggtacgg caagcattac tgaggttcgc caccgtctta atcggcgaat tgattgggaa
    12181 accacggtga gcattggttt acaacatgaa tatttgccga tattacctga tgctcccgaa
    12241 ctacatattg cgacagtagc gaaatatcag caggacagtg cggtgttaaa ccgtatcccc
    12301 attattctgc cggtactgaa tcgtcccaat gaatttttgt gggccagatt ggggaaacct
    12361 tatgctagcc atgaaagcgg tttctgtttt tacccagagc caggtgacga agttattatt
    12421 ggtttttttg aaaatgatcc gcgttatcca gttattttag gtgctatgca taatccgaaa
    12481 aataaggccc cttttgaacc aacccaagat aatagggaaa aagtattgat cgttaaaaaa
    12541 ggtgaagcgc aacaacaatt agtcattgat ggcaaagaga aaatgatccg aattaatgcg
    12601 ggtgaaaatc aaataatgct tcagcaagat aaagacattt ctctgtcaac gaaaaaagaa
    12661 ttaacactga aagcgcagac aatgaatgcc acgatggata aatcattggc aatgtccggg
    12721 aaaaacagtg ttgaaatcaa aggcgcaaaa attaatctta cccaatgaaa ggtgacgatg
    12781 aatggaaaat caaatactga cacaactcta tggtcgtggt tgggcttttc ctccggtctt
    12841 ttcccttgaa aagggggtag agatggctga aggggcggaa gatgtgagac aaagtttgca
    12901 gattctgttt agtactgagc cgggggaacg tcttatgcgt gaaaattatg gctgcggatt
    12961 aaatgatttt atgtttgaaa atatccgcaa tgaacttatt gctgaaattg aatcccatat
    13021 ccatgacaac gtattacgat atgaaccccg ggctgatatg actgatattc aggttcgtca
    13081 atcccctggc atggggaata ctttgcaagt gcaggtcatg tatcgcctga gagggagtga
    13141 tatcaatcaa caaatccagg gagtacttgc actgagtgaa ggccgggtga cggaggtagt
    13201 atgagtgaag cgattgtggt ggatggtgac gtgttacagt ttgatcccaa ctttggcaat
    13261 cggcaggtga cggttcccag cccaggaaaa attagcggca caggacatgc gcaggtaagt
    13321 ggaaaaaaag tgtgtattct gggggatgag aaacaggtca gggtttctgc aacctatatt
    13381 acaacaacac atactacgcc gggaacagga accattacta tcagtgctct ggatgctggc
    13441 cagcaggccc ttcagtgtac cagtggggcg gctttaatta tcaaggggca gcaatttacg
    13501 gcgatgttta cgcctgaatt gccagccatg aataatacag tgactccgcc acaaccggat
    13561 gttacgacac cttcatcagg aaaaggacgt tttatcactc aacaaaattt tgctaccgta
    13621 aatggtggtt caggtggtgg tggtagcggt ggtggcggtt ctggcggtgg cggtagtggc
    13681 ggaggtggaa gcggaggcgg tggaagtggc ggtgcaccga aaaagaaacg caaagttggt
    13741 attcatggtg ttccggcagc aatggataag aaatactcaa taggcttaga tatcggcaca
    13801 aatagcgtcg gatgggcggt gatcactgat gaatataagg ttccgtctaa aaagttcaag
    13861 gttctgggaa atacagaccg ccacagtatc aaaaaaaatc ttataggggc tcttttattt
    13921 gacagtggag agacagcgga agcgactcgt ctcaaacgga cagctcgtag aaggtataca
    13981 cgtcggaaga atcgtatttg ttatctacag gagatttttt caaatgagat ggcgaaagta
    14041 gatgatagtt tctttcatcg acttgaagag tcttttttgg tggaagaaga caagaagcat
    14101 gaacgtcatc ctatttttgg aaatatagta gatgaagttg cttatcatga gaaatatcca
    14161 actatctatc atctgcgaaa aaaattggta gattctactg ataaagcgga tttgcgctta
    14221 atctatttgg ccttagcgca tatgattaag tttcgtggtc attttttgat tgagggagat
    14281 ttaaatcctg ataatagtga tgtggacaaa ctatttatcc agttggtaca aacctacaat
    14341 caattatttg aagaaaaccc tattaacgca agtggagtag atgctaaagc gattctttct
    14401 gcacgattga gtaaatcaag acgattagaa aatctcattg ctcagctccc cggtgagaag
    14461 aaaaatggct tatttgggaa tctcattgct ttgtcattgg gtttgacccc taattttaaa
    14521 tcaaattttg atttggcaga agatgctaaa ttacagcttt caaaagatac ttacgatgat
    14581 gatttagata atttattggc gcaaattgga gatcaatatg ctgatttgtt tttggcagct
    14641 aagaatttat cagatgctat tttactttca gatatcctaa gagtaaatac tgaaataact
    14701 aaggctcccc tatcagcttc aatgattaaa cgctacgatg aacatcatca agacttgact
    14761 cttttaaaag ctttagttcg acaacaactt ccagaaaagt ataaagaaat cttttttgat
    14821 caatcaaaaa acggatatgc aggttatatt gatgggggag ctagccaaga agaattttat
    14881 aaatttatca aaccaatttt agaaaaaatg gatggtactg aggaattatt ggtgaaacta
    14941 aatcgtgaag atttgctgcg caagcaacgg acctttgaca acggctctat tccccatcaa
    15001 attcacttgg gtgagctgca tgctattttg agaagacaag aagactttta tccattttta
    15061 aaagacaatc gtgagaagat tgaaaaaatc ttgacttttc gaattcctta ttatgttggt
    15121 ccattggcgc gtggcaatag tcgttttgca tggatgactc ggaagtctga agaaacaatt
    15181 accccatgga attttgaaga agttgtcgat aaaggtgctt cagctcaatc atttattgaa
    15241 cgcatgacaa actttgataa aaatcttcca aatgaaaaag tactaccaaa acatagtttg
    15301 ctttatgagt attttacggt ttataacgaa ttgacaaagg tcaaatatgt tactgaagga
    15361 atgcgaaaac cagcatttct ttcaggtgaa cagaagaaag ccattgttga tttactcttc
    15421 aaaacaaatc gaaaagtaac cgttaagcaa ttaaaagaag attatttcaa aaaaatagaa
    15481 tgttttgata gtgttgaaat ttcaggagtt gaagatagat ttaatgcttc attaggtacc
    15541 taccatgatt tgctaaaaat tattaaagat aaagattttt tggataatga agaaaatgaa
    15601 gatatcttag aggatattgt tttaacattg accttatttg aagataggga gatgattgag
    15661 gaaagactta aaacatatgc tcacctcttt gatgataagg tgatgaaaca gcttaaacgt
    15721 cgccgttata ctggttgggg acgtttgtct cgaaaattga ttaatggtat tagggataag
    15781 caatctggca aaacaatatt agattttttg aaatcagatg gttttgccaa tcgcaatttt
    15841 atgcagctga tccatgatga tagtttgaca tttaaagaag acattcaaaa agcacaagtg
    15901 tctggacaag gcgatagttt acatgaacat attgcaaatt tagctggtag ccctgctatt
    15961 aaaaaaggta ttttacagac tgtaaaagtt gttgatgaat tggtcaaagt aatggggcgg
    16021 cataagccag aaaatatcgt tattgaaatg gcacgtgaaa atcagacaac tcaaaagggc
    16081 cagaaaaatt cgcgagagcg tatgaaacga atcgaagaag gtatcaaaga attaggaagt
    16141 cagattctta aagagcatcc tgttgaaaat actcaattgc aaaatgaaaa gctctatctc
    16201 tattatctcc aaaatggaag agacatgtat gtggaccaag aattagatat taatcgttta
    16261 agtgattatg atgtcgatca cattgttcca caaagtttcc ttaaagacga ttcaatagac
    16321 aataaggtct taacgcgttc tgataaaaat cgtggtaaat cggataacgt tccaagtgaa
    16381 gaagtagtca aaaagatgaa aaactattgg agacaacttc taaacgccaa gttaatcact
    16441 caacgtaagt ttgataattt aacgaaagct gaacgtggag gtttgagtga acttgataaa
    16501 gctggtttta tcaaacgcca attggttgaa actcgccaaa tcactaagca tgtggcacaa
    16561 attttggata gtcgcatgaa tactaaatac gatgaaaatg ataaacttat tcgagaggtt
    16621 aaagtgatta ccttaaaatc taaattagtt tctgacttcc gaaaagattt ccaattctat
    16681 aaagtacgtg agattaacaa ttaccatcat gcccatgatg cgtatctaaa tgccgtcgtt
    16741 ggaactgctt tgattaagaa atatccaaaa cttgaatcgg agtttgtcta tggtgattat
    16801 aaagtttatg atgttcgtaa aatgattgct aagtctgagc aagaaatagg caaagcaacc
    16861 gcaaaatatt tcttttactc taatatcatg aacttcttca aaacagaaat tacacttgca
    16921 aatggagaga ttcgcaaacg ccctctaatc gaaactaatg gggaaactgg agaaattgtc
    16981 tgggataaag ggcgagattt tgccacagtg cgcaaagtat tgtccatgcc ccaagtcaat
    17041 attgtcaaga aaacagaagt acagacaggc ggattctcca aggagtcaat tttaccaaaa
    17101 agaaattcgg acaagcttat tgctcgtaaa aaagactggg atccaaaaaa atatggtggt
    17161 tttgatagtc caacggtagc ttattcagtc ctagtggttg ctaaggtgga aaaagggaaa
    17221 tcgaagaagt taaaatccgt taaagagtta ctagggatca caattatgga aagaagttcc
    17281 tttgaaaaaa atccgattga ctttttagaa gctaaaggat ataaggaagt taaaaaagac
    17341 ttaatcatta aactacctaa atatagtctt tttgagttag aaaacggtcg taaacggatg
    17401 ctggctagtg ccggagaatt acaaaaagga aatgagctgg ctctgccaag caaatatgtg
    17461 aattttttat atttagctag tcattatgaa aagttgaagg gtagtccaga agataacgaa
    17521 caaaaacaat tgtttgtgga gcagcataag cattatttag atgagattat tgagcaaatc
    17581 agtgaatttt ctaagcgtgt tattttagca gatgccaatt tagataaagt tcttagtgca
    17641 tataacaaac atagagacaa accaatacgt gaacaagcag aaaatattat tcatttattt
    17701 acgttgacga atcttggagc tcccgctgct tttaaatatt ttgatacaac aattgatcgt
    17761 aaacgatata cgtctacaaa agaagtttta gatgccactc ttatccatca atccatcact
    17821 ggtctttatg aaacacgcat tgatttgagt cagctaggag gtgacaaacg tccggcagca
    17881 accaaaaagg caggtcaggc caaaaagaaa tagagtattg actgaattaa atagaattaa
    17941 cgaaggtgta aataattatt tatttgctga cgaatcgctg tgacaaataa acacaggtga
    18001 tgttatggaa ttaaatgagt taactaacaa attgtcaaat ttggtgccaa tgaccgattt
    18061 taaattagat aatcgagcca gtttgcaatt gcttaaatat attgaagcgt atacgaagat
    18121 aatacccttt aattctggcg ataaatattg gaatgacttt ttctttatgt caggaaatac
    18181 gccagagaaa cttgcaaaat tatatcagaa agaaatagaa cccaatgggg agttattacc
    18241 tcagcaggct tttttgttgg cggttttgcg tttattggaa acaccaatat ccttattaaa
    18301 tgtattacct gctgctcatc gtgagctcta ttatcgggag cttttaggct tgtcttccca
    18361 tgcggcacag cctgatcagg ttgctttatc tatggaactg aattcgacag tgatggaaca
    18421 gctgctccct gaaggaaccc tgtttgaggc tggtcaggat gaacaaggca atgcattgca
    18481 atatgccctg gatgccagtt tgctggctaa tcgtggatat atcagtgact tgcgctggtt
    18541 acggaatgac ggggaaaagc aatgggttac ttctgctcca tgggatttac aggcacaggt
    18601 gtcactgccg tctgatggga tacgattatt tggtaagaca aatagtgatc agcaggtatt
    18661 tggtggggtg ttgataacgt catcacttct ggcgatggaa gcggggataa ggaagatcat
    18721 tgttactttt gagcaggaga tgaacaccca agaactggtg gcacaggtca gcagtggaaa
    18781 tcaatggcta acattgacgt ctgaggtaaa taagaaagag gtcacactga cactgtcaga
    18841 caaagaaccg gcaatcagtg cgccagagga tctggataat ctctttttca cgcaaccggt
    18901 actcaggcta cagggaaagg atagtcaggc actgccggag gtgacgggta tcagcgtttc
    18961 ggaaaaggat gatactaagg atacctcttt tgagatgtat cacttaacac catttggtta
    19021 tagcagtgat atagagccat tggaggaaaa tccagcgtta tatttaggct ttactgatgt
    19081 aaagccaggg caaacactgg cgctgtattg gaaattaaaa tccccgcagc aaccaaccgt
    19141 ttcctggtat tacctggatc aacataatca atgggctgaa ttggattcat gggtcagtga
    19201 tggaacccag aatctgtatc aggatggtac ttggcacgtt gagttgcctg tggatgcatc
    19261 caatcaggca gagcagatgc cagttggacg ctattggttg cgggcagtgg tggaggtacc
    19321 cgctcatgag ggggcgttgg ggaaggctcc ttggctatat ggtctaatct ataacgccat
    19381 gacggcaacc ttggttaatg tagatagcat cagtgacagc catttcttaa cccctttgcc
    19441 tgccagcagc atacagcggc ccgttgaacc catcattgtg ttggcatcgg tcaaccagcc
    19501 ttgggcatca tggggtggac gtatacctga atcctacagt gccttttttg aacggatagc
    19561 tcaaaacctg tctcatcgaa accggtcctt aacctgggga aatatggtga cattactcaa
    19621 agagcgttat gtcagcatct ttgatgttaa gtatccaggt aatgatgaac tcaccagagt
    19681 gccagcattg gagcagcagc aactaacagt gattccagca aaccggtaca acgatagcga
    19741 tgattctctg cgtccggtac tgaatcctgc tcgtctgcaa gagatggctg attggttgca
    19801 gcagaaagac tctccctggg cctctattga ggtcaggaat ccagaatact tggatgtgaa
    19861 aatccattac gaggtgattt ttaaacctga tgtgaacgaa gattttggct atcgccagct
    19921 acagcagcaa ctgtgtgagg tgtatatgcc ttggagcata gatgagcagc ggcccgttgt
    19981 attgaataac agcattaatt atttccagtt gttagccact attcaacagc aaccgctggt
    20041 tgagcgagtc actcgtctga cactacatcg ggctgattct tctgatgaga gtgatggtac
    20101 agcatctgtg gaagccaaag ataatgaagt gcttatttta gtctgggaag aggacgataa
    20161 tctgcaatac cgaggaaatg actatgagta atcaggatgc actgtttcat agcgttaaag
    20221 acgatattca ctttgatacc ttgctggaac aagctcatca ggtgattgaa aaacaggctg
    20281 aaaaactgtg gagtgatacg gcagagcatg atccgggtat cacatttttg cagggaatca
    20341 gttacggtgt gtcagatttg gcttaccgac atacattacc cctgaaagat ttactgactc
    20401 cggcgccgga tgagcagcag caagagggaa tttttcctgc cgaatttggc ccgcataata
    20461 cactgacttg tgggccggtg acagcggatg attatcgcaa ggcattgtta gatctacaca
    20521 gcagcgacag cctggatggt actcagcagg atgaggggga ttttctgttc cggagtgtgc
    20581 aactggtgcg tgaaccggaa aaacagcgtt atacctattg gtatgatgca accaagaggg
    20641 aatatagctt tgtcaacagt gaaggggcta aagagtttac cttgcggggg aattactggt
    20701 tgtatctgga accaacccgt tggactcagg gtaatattgc cgctgctacc agacaactga
    20761 cagaattttt gactaaaaat cgcaatattg gtgaatctgt cagcaacatt atctggctac
    20821 aaccggttga tctgccactg ttgctggatg ttgaactgga tgatgatgta ggtgcacagg
    20881 atgtccccgg tatttttgcg gcggtgtata gcaccgcaga gcagtatctg atgcctggag
    20941 cacagcgtta ccgtacggaa gtactgcaaa atgctgggat gagcaatgat caaatcttcg
    21001 aaggtccatt attggaacat ggctggatac cagagctgcc ggcagcccgt gattatactc
    21061 aaaggctcac tctcaatctt agccggttgg taaatagtct gcttgagatt gagggcatta
    21121 aacatgtgaa tcgtcttcgt ctggatgata gcttcgataa aactgctatt gaacccgtta
    21181 agggggatac ctggtcgtgg tcgatcaaag agggctatta tccacgtctt tggggagaag
    21241 acccacttaa ccaattggcg caacaaaatg gcccgcttag ggtgatagcc aaaggaggga
    21301 ttagcgtcag tgtgagtaaa gagcaaatcc aggccagttt acccagtcaa tcactgattc
    21361 aaaatgagcc ggtaatattg gcttacggcc agcaccgtga cgttggcagc tattatcccg
    21421 tcagtgatac tttgccgcct tgctatggac tacaacattc tttgtctgaa agtgaacact
    21481 tattgccact tcatcaattt atgttgccat ttgaacaatt attggcctgt ggttgtcaac
    21541 agatagccat gctcccgcgg ttactggctt ttcagcgcga aggttatgag gtttggggtg
    21601 atcagtggcc ctttaagtca ggctcagtga atgatgacgc ccatcaagat tatgcccctg
    21661 cattaaagga tttgttagga cagattgcgc tggatagtga tcatgaattg gatattatta
    21721 attacttgct gggttacttt ggcacacagc gggcaccgcg tacctttacg acacaactcg
    21781 atgattttcg tgcggtccaa cagggttatc tggcccagca accgacattg acttaccacc
    21841 gctccaatat tcgtatcgat caggtatcgt cgctacaaaa acgtattgct gctcgcatgg
    21901 ggctgggcgg tgagttgttt aaacctcaac cggatctgag ccaactgcct ttttatttga
    21961 ttgaacatcg agcgttgctg ccagtcaaac ccaatagtca gtttgataag gaacagaaac
    22021 cagcctcggt gacagaggag gggggcagcc aaacaggtca acattatgtg gtcattgaac
    22081 agaagggcat tgatggcaag ctgacacagg ggcaagtgat caatttaatt ctgtatgaag
    22141 gagagcaggg agaaacccaa tttacgatac gcggtcagat ggtattcaaa accgaggggg
    22201 ataagttttg gttggatgtg aataatagtg cgcaactgga atataatctg gcgcgggtaa
    22261 tgacagcagc caaggcgagt aaactctttt ggcaaaacag cccggtatgg atggaggata
    22321 tgggctatcg tctggcctat gctagtgacc aatcctcatt gcctgtgaat caacggcgct
    22381 tgacccgcac agtgcaaact ccattcccgc cgatggttgt tgtaggtagc gaaatcaccc
    22441 tgttaaagca ggtggggata gtcaatttaa aaaaagcgga gtcagaaaaa ctttatgcaa
    22501 aagttgttag ctttgatcgc attgaaggga ccttgattat tgagcgtttg ggtaattcca
    22561 ctctggcttt tcctacctcg gaagaggcgt ggcggtatag ttggtatttt tcgggggaga
    22621 aatatgaaag gactgaccgc ttttcatttg tgattagcgt agtagtgaac agtgacttaa
    22681 ttaaattgcc cggtgttgat ccctataaat tggaagaatg ggtgaaagaa acgattctta
    22741 ccgaatttcc agctcatatt tctatgatta tccattggat ggatcgggaa gcctttttaa
    22801 atttcgccaa tacctatcag cgttggcaaa ataatggtac gccactgggg gatgcggctt
    22861 attccattct agaaagtttg acacttggta aattgccatc tgccttaaaa ggtgttggca
    22921 caatgcgtat tgccacatct agtcaaagag aagaagtggt gggtagtaat ggtgatcaat
    22981 ggaatacaga tggaataacc cagaatgaat tattctatgt tcctaaagag agctaggaaa
    23041 aataaatatc tgccactaat gatgttgaat taaatatgtt ttctggagtt aatcatgaac
    23101 gaaactcgtt ataatgcaac tgtacaagaa caacaaacat tatctaatcc aaaagctgtt
    23161 ggacctgaca tcgataaatt aaaggataaa tttaaagagg gcagtattcc cctgcaaacc
    23221 gatttcaatg agttaattga tattgccgat attggacgta aagcctgtgg tcaagcgcca
    23281 caacaaaatg gcccaggaga aggattgaaa ttggctgatg acggtacgct taatttaaaa
    23341 ataggcactt tttccaataa agacttttct ccattaatat taaaagatga tgttttatct
    23401 gtagatcttg gtagtggtct gactaatgaa accaatggaa tctgtgtcgg tcagggcgat
    23461 ggtattacag ttaacactag caatgtagct gtaaaacaag gtaacggaat tagcgttact
    23521 agtagtggtg gtgttgccgt taaagttagt gctaataagg gacttagcgt tgatagtagt
    23581 ggtgttgcag ttaaagttaa tactgataag ggaattagcg ttgatggtaa tggtgttgca
    23641 gttaaagtta atactagtaa aggaattagc gttgataata caggtgttgc agttatagct
    23701 aatgctagta agggaattag cgttgatggt agtggtgttg cagttatagc taatactagt
    23761 aaaggaatta gcgttgatgg tagtggtgtt gcagttatag ctaatactag taaaggaatt
    23821 agcgttgata atacaggtgt tgcagttata gctaatgcta gtaagggaat tagcgttgat
    23881 ggtagtggtg ttgcagttat agctaatact agtaaaggaa ttagcgttga tggtagtggt
    23941 gttgcagtta tagctaatac tagtaaagga attagcgttg atagtagtgg tgttgcagtt
    24001 aaagttaaag ctaatggcgg aattaaagta gatgctaatg gtgttgcaat tgatcctaat
    24061 aatgtactcc ccaagggagt gattgtaatg ttctctggca gtactgcacc aactggttgg
    24121 gcgttatgtg atggcaataa tggtacacca aatttaatcg atcgatttat tttaggtggg
    24181 aaagggactg atattaatgg agtgagtact aatacagctt caggtactaa aaatagtaag
    24241 ttattcgatt tcagttctga tgaagctaca ttaactattg atggtaaaac actggggaga
    24301 ggcggttctg gcggcggtgg ttctggcggt aacgataaac tgaccctgtg gaccacaccg
    24361 gcaccgagtc cgaattgtcg tctgaatgca gaaaaagatg caaaactgac actggttctg
    24421 accaaatgtg gtagccagat tctggcaacc gttagcgttc tggcagttaa aggtagcctg
    24481 gcaccgatta gcggcaccgt tcagagcgca catctgatta ttcgttttga tgaaaatggc
    24541 gtgctgctga ataatagctt tctggatccg gaatattgga attttcgtaa tggtgatctg
    24601 accgaaggca ccgcatatac caatgcagtt ggttttatgc cgaatctgag cgcatatccg
    24661 aaaagccatg gtaaaaccgc aaaaagcaat attgttagcc aggtgtatct gaacggcgat
    24721 aaaacaaaac cggttacact gaccattaca ctgaatggca cccaagaaac cggtgatacc
    24781 acaccgagcg cctatagcat gagctttagc tgggattgga gcggtcataa ctatatcaat
    24841 gaaatttttg cgaccagcag ctacaccttt agctatattg cacaagaagg tggctctggt
    24901 ggtggtggtt caggtggtca cgatcatgat attaagataa cgggcacagg aaaacattct
    24961 cacaaaaaca aagtaacagt cccttattat attctggctt tcatcataaa gctttaatat
    25021 atatgaaaaa ttgaaaatat aaattatcca ttaataataa agaggatatt agcatgactt
    25081 cggagccaaa tctgttaaac cggattacaa ttactattga agctaataat caacaagtag
    25141 ctagaaaagt attgcatggc tccttgctta atcaagctaa tataaataaa ttatttaatt
    25201 catactttaa tgaatatgaa attaataggg gtgtttattt agaaacatta atcctgaatc
    25261 ttggtacgat aaatttccat gattttaatt cattgtttcc tactctccta aaagctgcat
    25321 tgaataaaga attcagtcaa tatcagataa acaaccatag ggaagaaatg ctatttaatg
    25381 agacaatatc aaatcaagct actgataagt cttacatatt tggcgataac aaattaattg
    25441 atgcagagaa tttcattcac tttttatatc aaaagcattc cacattaaat ctagtagaag
    25501 caatgggaaa taatggtatt gaaaaattaa caaatcagtt aacacaaata gaaaataaat
    25561 ttgcgttatt attggcaaaa agttgtttgt ctgaggaagg cttaaaacga ctcttggcta
    25621 tcaaacaacc cgatttatta atcgctatca atcgcagatt atctgaaaga ataaatagac
    25681 cacaatatca ggagaagctt gtttcctgcg gacaactgat atttagtgct ctgggatata
    25741 tacaacagta caatatacag gaaattccta aaccggatga aaaagttatt gcacgcataa
    25801 caactgaact taataataat ggtttgctta atacaatacc tattattaca ctatttcgtc
    25861 agagtgggat taacgattca tcactaaatg attggctaaa gaaaatctgg caggtgagat
    25921 caatttcaca gttatgcaga aagtatcttt ctgctaagga ataccaatat ctgtcagaac
    25981 attttgtttc aaagagcgtc gataaaaata gatatgatga agagcccgta aatcagagca
    26041 tattatcaag gttgaataat aattccatta aagaaggaaa taatcacagt caactctgta
    26101 ctctcagtag actatattct gaacccgttg tattacctga acaaaccatt ctacgtcagg
    26161 ttagtaatac agtagatcag agcatattat caaggttgaa taatgcctcc attaaagaag
    26221 gaaataacca aagtcaactt cgcactctca gtagactata ttctgagccc gttgcattac
    26281 ctgaacaaac cattccacgt caggttagta atacaggtat attaattcta tggccaatgc
    26341 tacctacact atttaaccag cttggtctac ttgagaaaaa gaaatttatc catcgtcagg
    26401 cccagtttaa tgccgttgat tttcttgatt acctgatttg gggaaccgaa gatgtgaaag
    26461 tggaacgaaa ggttttgaat aatgttctat gtgggttaat ggctgatgaa attactgaac
    26521 caatgcctat tgaaccagaa aaacaatgga taataattca atggctggac gctattatct
    26581 cccaactttc tggctggaaa aagttaagtc gtaatgacgt ccgtcaatta tttctacaac
    26641 gaccaggaga attactgatc aatgaacagg aaattaaaat cacaatacag caacaaccat
    26701 ttgatgctct gttaactgat tggccgtggc caatgaatat ggcttgtttt agctggttga
    26761 gtcaaccatt aaccattacg tggttataac cattgaccac aatgacttag tctgagtaaa
    26821 aaatatgaat atatcgcctg ttttttatga ttcattgaat caggataacg accgtgatct
    26881 atcgttttta tttagcgaac tggaacgaat agatctcgct cttcaacacc atttttattg
    26941 tgtagaaagt cagcgaagtg agctcctgga tgagtttctg ctcactgagg cggaagtggt
    27001 gaccaggctg gataagccac ttggtaaacc tcattggata aatgatgatt atctggcgat
    27061 atcgcaaaag ggcaatgtaa gcctaatggc agcgtccaga ttaatggatc tgatcgaacg
    27121 ctttgaactg actgattttg agcgcgatgt tttactatta ggcttattgc cccattttga
    27181 tagccgctat tatcgactgt tttcgctgat tcaaggggga caacagggtc gattaccttc
    27241 ttttgcgctg gcattggaac tgttttgcca ctcggcgctg gagaaacagg tacagcaagc
    27301 gagttttctg caccgggcac ctttgatggg ttgccagcta ttatccatcg atactagtca
    27361 aaaaacgctg gcctggctcc agactccctt tattactgac agcggggtat atcacttttt
    27421 actggggcat cactacatta tgccggcttt agaacattgt gctgagtggt taacaccgac
    27481 agggattggc tgttatcctg aaggattaaa acaagtactg ggtaacgtat tgttatctga
    27541 caacgataat attagaccga ttgtcttatt acggggaatg gccggcagtg ccagagctta
    27601 taccattact aatatgatgg cttcagaagg gaagcaaaca ctgctggtag atatatccaa
    27661 acttgctgat agcgatgaaa aaaacattat tcttcagata aagcatattt tgcgggaaac
    27721 ccgcatgcat ggagcatgtt tattattacg gaatttttgc ttgttagtgg aacagaataa
    27781 acaactattg gactccctgt cagagttatt gaatcaacct gaattaagaa ttgtttgcct
    27841 gattgagcct tattccccat tggtatggct gaaaaagata ccggtattac tgattgagat
    27901 gccactttta acgcctgcgg aaaaagccag attgttaatt gccagcttac cggataattg
    27961 ttccgaggat attgatacga taactttaag ccagcgttac acttttaacc cagaaaccct
    28021 gccattgatt ttgcaagagg cccagcttta tcaacagcag cgagatccgc tggatatctt
    28081 gcagcaatgc gatatacgcc aggcattaaa tttgcgtgct caacaaaatt tcggtcaatt
    28141 ggcacagcgg attattccta agcgctcatt aaaggattta ttggtatccg atgagattgc
    28201 tcagcagtta cgggaaatac tcatagcaat taagtatcgg gaacaggttc tggcgggagg
    28261 gtttaaagat aaaattgcct atggcactgg tatcagcgcc ctgttttatg gtgattcagg
    28321 cactggaaaa accatggcag cagaagtgat tgctgaccac attggcgttg acttaataaa
    28381 agtggattta tctacagtag tgaataaata catcggtgaa acagaaaaaa acttatcccg
    28441 tattttcgat ttggcggaac aggatgcagg ggtattattc tttgatgaag ctgacgcact
    28501 gtttggtaaa cgcagtgaaa ctaaagattc ccaggacaga catgccaata ttgaagtttc
    28561 ttacttatta cagcgcctgg agaattaccc gggtctggtc attttatcca ccaataatcg
    28621 tggtcattta gacagtgctt ttaatcgtcg ttttactttc attacccgtt ttacttaccc
    28681 ggatgaaaaa atccgtaaaa aaatgtggca ggaaatttgg cctagaaata taaaaatatc
    28741 ggaagatatc gattttaacg aattagctca acgaacaagc gtgactggcg cgaatatccg
    28801 caatattgct ttattgtctt cattctttgc ttcagagcag gggaatgatg aagtcagtaa
    28861 tgaaaatatt gaaattgcat tgaagcgtga attagctaaa gtcggacgat taacatttta
    28921 aaagttatca caatgaaagt attgaaatat taaataaatt tattaccaaa aagttatcac
    28981 gatataattt aagagaggtt ttttatgtta aacacgcaaa ctattattga tgtcaataag
    29041 gcaatggatg ccatgctgcg cgcatatctg aatcaagata ttgccattcg ttttgatcta
    29101 cctgaattgg atactatgca atctgatgcg atggtaagta tctttcttta tgacattcat
    29161 gaagatttac agcttcgctc ggcagaatca agagggtttg atgtttatgc cgggaggtta
    29221 ttgcctggtt gggtaaatat taaatgtaac tatctgatta cctattggga agcttctaag
    29281 ccagcgactg atgccagcag tccggatagc caacctgata accaggcaat acaagtgatg
    29341 tcacaagtat taaatgcctt gattaataat cgtcaattgg caggtattcc tggtgcttat
    29401 actcaggttg taccgcctaa agagagttta aatagcctgg ggaatttctg gcaatcactg
    29461 ggtaatcgcc cacggctttc tctcaattat tcagtgacag tacctgttag cctaaacgat
    29521 ggtcaggata gcgcgactcc ggttaccgcg gtttcttcta cagtggaaca aacggcatcg
    29581 ctcagtcaag aagtggttag tcatgcttta cgcgaattac tcattacgga attaggagga
    29641 ggagaggata accggttggt actgagtaaa gttgaattat ccgcagtgaa agagacgatg
    29701 actcaagaca gtccggctca gatgattata ttgttgtctg tttcaggcat tacacgacag
    29761 gaatatttga aggaaattga taatatcttt gatcgttggg taaataatgc tgaagttatt
    29821 accactattg atgattgtgg gattagaatt gaaagtataa cgaaagataa tcttgtagga
    29881 atttaaattt ttttaattaa ataaaggaat taatattgat ttgatagagc atgtaatcaa
    29941 taacttctat gaaaatgttg aaaaacaata cacggaaaca cttaacttac aattaaattg
    30001 ttaaag
//
