LOCUS       sequence-484643-       12500 bp    DNA     circular SYN 26-AUG-2025
DEFINITION  Insert coding for mouse RBP2 (accession ID NP_001074857.1) with an 
            N-terminal mKATE2 with a p2A cleavage site cloned inframe into a 
            f(syn)w-mKATE2-p2A. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12500)
  AUTHORS   Kapoor R, Schwenzer N, Dresbach T, Lehnart SE, Moser T
  TITLE     Establishing synthetic ribbon-type active zones in a heterologous 
            expression system
  JOURNAL   eLife13:RP98254
REFERENCE   2  (bases 1 to 12500)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 26, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "eLife13:RP98254"
COMMENT     Sequence Label: f(syn)w-CMV-mKate2-p2A-RimBP2
FEATURES             Location/Qualifiers
     source          1..12500
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(24..43)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        215..594
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        596..794
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     749..769
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             812..992
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1039..1164
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1657..1890
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2075..2119
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2417..2534
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2603..3050
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      3066..3075
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3072..3767
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     CDS             3777..3833
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7077..7106
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    7169..7757
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7222..7242)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7640..7651)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7760..7776)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7761..7777)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8282..8462
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8488..8505)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8494..8718
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8764..9192
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8851..8870)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9061..9082
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9201..9221)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9206..9535
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9386..9521
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9448..9467
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9583..9630
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9649..10023
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10153..10274
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10190..10209)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10244..10263
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10323..10339)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10323..10339)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10336..10358)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10347..10363
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10371..10377,10378..10395,10396..10401))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10416..10437
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10554..10571)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10725..11313)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10805..10824)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11484..12275,12276..12344))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12107..12126
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12345..12449)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
       61 ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca
      121 acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg
      181 ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa
      241 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
      301 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
      361 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
      421 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
      481 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
      541 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
      601 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
      661 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
      721 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
      781 gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc tggttagacc agatctgagc
      841 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
      901 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
      961 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     1021 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     1081 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     1141 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     1201 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     1261 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     1321 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     1381 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     1441 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     1501 gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     1561 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     1621 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     1681 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     1741 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     1801 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     1861 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     1921 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     1981 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     2041 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     2101 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     2161 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     2221 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     2281 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
     2341 cagatccatt cgattagtga acggatcggc actgcgtgcg ccaattctgc agacaaatgg
     2401 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
     2461 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
     2521 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg ttaatcgcat
     2581 gctagagagg gccctgcgta tgagtgcaag tgggttttag gaccaggatg aggcggggtg
     2641 ggggtgccta cctgacgacc gaccccgacc cactggacaa gcacccaacc cccattcccc
     2701 aaattgcgca tcccctatca gagaggggga ggggaaacag gatgcggcga ggcgcgtgcg
     2761 cactgccagc ttcagcaccg cggacagtgc cttcgccccc gcctggcggc gcgcgccacc
     2821 gccgcctcag cactgaaggc gcgctgacgt cactcgccgg tcccccgcaa actccccttc
     2881 ccggccacct tggtcgcgtc cgcgccgccg ccggcccagc cggaccgcac cacgcgaggc
     2941 gcgagatagg ggggcacggg cgcgaccatc tgcgctgcgg cgccggcgac tcagcgctgc
     3001 ctcagtctgc ggtgggcagc ggaggagtcg tgtcgtgcct gagagcgcag tcggatccac
     3061 cggtcgccac catggtgagc gagctgatta aggagaacat gcacatgaag ctgtacatgg
     3121 agggcaccgt gaacaaccac cacttcaagt gcacatccga gggcgaaggc aagccctacg
     3181 agggcaccca gaccatgaga atcaaggcgg tcgagggcgg ccctctcccc ttcgccttcg
     3241 acatcctggc taccagcttc atgtacggca gcaaaacctt catcaaccac acccagggca
     3301 tccccgactt ctttaagcag tccttccccg agggcttcac atgggagaga gtcaccacat
     3361 acgaagacgg gggcgtgctg accgctaccc aggacaccag cctccaggac ggctgcctca
     3421 tctacaacgt caagatcaga ggggtgaact tcccatccaa cggccctgtg atgcagaaga
     3481 aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc cgctgacggc ggcctggaag
     3541 gcagagccga catggccctg aagctcgtgg gcgggggcca cctgatctgc aacttgaaga
     3601 ccacatacag atccaagaaa cccgctaaga acctcaagat gcccggcgtc tactatgtgg
     3661 acagaagact ggaaagaatc aaggaggccg acaaagagac ctacgtcgag cagcacgagg
     3721 tggctgtggc cagatactgc gacctcccta gcaaactggg gcacagagga tccggagcta
     3781 ctaacttcag cctgctgaag caagcaggag atgttgaaga aaaccccggg cctgctagca
     3841 tgcgagaggc tgctgagcgg cggcaacagc tggagttgga gcatgagcaa gccctggcct
     3901 tcctcaatgc caagcagcag gagatccagc tactgcagca ggctcaggtt gaagctaaga
     3961 aagagcatga aggtgctgtg cagctgttag agaacaccct ggactgcatg cagtccaagg
     4021 tccgagagct ggaagagaaa tgccgtgtgc agagtgagca gtttaacttg ttgtcccggg
     4081 acctggaaaa gttccggcaa cacactggga gtattgacct gctgggcagc agctcagtgg
     4141 ccctgctgga tgttcccttg gcccctggca agcctttccc tcagtacatg aatggactag
     4201 ccacctccat ccacaaaggt cacgagggcc ccactggaca ctactctgtg attggtgact
     4261 atattccgct gtctggggac aagctggagt ctccgtgtgt gaagccctcc ttcctcttgc
     4321 gatccagcag cccaagatgc agatttgagt ccgagatgga caatgaccgg aactctaaca
     4381 actccaagca aagcagctcg gggaaggtgc acctgtgtgt ggcccgctac agttacaacc
     4441 ccttcgatgg gcccaatgag aacccagaag ctgagctgcc tctcacggcg ggaaagtacc
     4501 tctatgtcta tggggacatg gatgaagatg ggttctatga aggagagctt ctggatgggc
     4561 aaaggggctt ggtgccctcc aactttgtgg attttatcca ggacaatgag tcgcggctgg
     4621 ctggtactct ggggagtgag caggaccaga actttctcaa ccactctggc atcagtctgg
     4681 agcgcgacag catccttcac cttcactccc caactcaagt ggactcgggg atcaccgaca
     4741 atggtggggg gaccctggac gtgaacatcg acgacatcgg agaagacacc gtgccttacc
     4801 ctaggaaaat cacccttatc aaacagcttg ccaaaagtgt catcgtgggc tgggagcccc
     4861 ctgctgtgcc tcccggctgg ggcaccgtga gcagttataa tgtgctggtg gacaaagaga
     4921 cacgcatgag ccttgccctg ggtaggagaa ctaaggcgct gatcgagaaa cttaacacag
     4981 ctgcctgcac ctaccgcatc tccgtgcagt gcgtcacgag ccggggaaac tcagacgagc
     5041 tgcagtgcac tctgctggtg ggcaaggatg tggtggtggc accgtcccag ctgcgtgtgg
     5101 acaacatcac acagatctct gcccagctct cgtggctgcc gaccaacagt aactacagcc
     5161 atatcatctt cctcaacgaa gaagaactgg acatcgtgaa ggcagccagg tacaagtacc
     5221 aattcttcaa cctcaggcct aatatggcct acaaggtgaa ggtcttggcc cagccacacc
     5281 agatgccctg gcagctgccg ctggagcaga gagaaaagaa ggaggcctgt gtggagttct
     5341 ccacgctgcc tgcaggacct ccagccccac cacaagatgt cactgtccac gctggggcca
     5401 cagccgcctc tgttcaggtc tcctggaagc cccctgcact gactcccact gggttgtcca
     5461 atggagcaaa tgtcacagga tacggcgtgt acgccaaagg gcagagggtg gctgaggtca
     5521 tcgcccccac ggcagatggc acggcagtgg agctgatccg gctgcgaagc ctagaggcca
     5581 aggccgtgag tgtgcgtacc ctgtctgtac agggagagtc catggattct gccctcgctg
     5641 ccatcccccc tgacctcctg gtgcctccag ccccccaccc gaggactgct cccccaccaa
     5701 agccattagc aagtgacatg gataccaaag accaacacct ggggccccac gtcaaagtgg
     5761 atgagtcctg ggagcagagc cggtcaccgg gccctgcaca tggccacatg ttggaaccac
     5821 ctgacatgca cagcgctggc ccaggcagaa ggtcaccctc gcccagccgg atccttcctc
     5881 agccacaagg agcccccgtg tctaccactg tcgccaaggc catggcccgt gaagctgcac
     5941 agagggtggc tgagagcaac aggttagaga aaaggagcct ctttctagag caaagcagtg
     6001 cggggcaata taccaactcg gacgaggagg acggctacgc ctcccccgag gtcaagagga
     6061 gaggcacctc agtggatgac ttcctcaaag ggtcagagct gggcaagcag ccccactgtt
     6121 gccatggaga tgagtaccac acagagagca gccgggggtc agacctgtcg gacatcatgg
     6181 aggaggatga ggaggagcta tactcagaga tgcagctgga ggatgggggc cgccgtcggc
     6241 ccagcggtac ctctcacaac gccctcaaga ttttaggaaa ctccacgttg atgggacgag
     6301 cagaccggat ggaacacgtg agccgaaggt attcacacag tggcggaggg tctcataggc
     6361 accgcccagc gatggctcca tccattgatg aatacaccgg gcgagaccat ctttctccag
     6421 acttctatga tgagtccgaa actgaccctg gtgctgagga gctcccagcc cgtatctttg
     6481 tggctctgtt tgactatgac ccactgacca tgtccccaaa cccagacgct gctgaagagg
     6541 agcttccctt caaagaagga cagatcatca aggtatatgg agacaaagac gcagatggct
     6601 tctaccgtgg ggagacctgt gccaggctcg gcctcattcc ctgtaacatg gtctctgaga
     6661 tccatgcgga tgatgaggag atgatggatc agctgctgag gcaaggcttc ctccctctga
     6721 acacgcctgt ggagaaaata gagagaagta gaagaagcgg ccggggtcac tctgtaccca
     6781 cacgaagaat ggtggctctc tacgactatg atcctaggga aagctctcct aacgtggatg
     6841 ttgaggctga acttccattt tgcacaggag acattattac tgtttttggt gaaatcgatg
     6901 aagatggatt ttattatgga gagctgaatg ggcaaaaagg cctcgtgcct tccaatttcc
     6961 tggaagaagt gcctgatgat gtggaggtcc acctttctga tgctccgccc cactactccc
     7021 acgacccgcc catgcgctcc aaggccaaaa ggaagaagag tgttcatttc acacccgaac
     7081 aaaaacttat ttctgaagaa gatctgtaag gcgcgccttc gaattaatta aggccgcgac
     7141 tctagaattc gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag
     7201 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     7261 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     7321 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     7381 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     7441 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     7501 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     7561 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     7621 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     7681 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     7741 ttgggccgcc tccccgcatc gataccgtcg acctcgagac ctagaaaaac atggagcaat
     7801 cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga
     7861 ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc
     7921 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     7981 ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
     8041 ttggcagaac tacacaccag ggccagggat cagatatcca ctgacctttg gatggtgcta
     8101 caagctagta ccagttgagc aagagaaggt agaagaagcc aatgaaggag agaacacccg
     8161 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tattagagtg
     8221 gaggtttgac agccgcctag catttcatca catggcccga gagctgcatc cggactgtac
     8281 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     8341 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     8401 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     8461 cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     8521 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     8581 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     8641 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     8701 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
     8761 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     8821 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     8881 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     8941 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     9001 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     9061 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     9121 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     9181 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     9241 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     9301 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     9361 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     9421 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     9481 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     9541 cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg
     9601 gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg
     9661 accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc
     9721 gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac
     9781 gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc
     9841 tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg
     9901 aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg
     9961 gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac
    10021 tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
    10081 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
    10141 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
    10201 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
    10261 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
    10321 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
    10381 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
    10441 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
    10501 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
    10561 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
    10621 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
    10681 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
    10741 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
    10801 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
    10861 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
    10921 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
    10981 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
    11041 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
    11101 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
    11161 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
    11221 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
    11281 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
    11341 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
    11401 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
    11461 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
    11521 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
    11581 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
    11641 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
    11701 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
    11761 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
    11821 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    11881 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    11941 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    12001 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    12061 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    12121 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    12181 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    12241 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    12301 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    12361 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    12421 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
    12481 gacggatcgg gagatctccc
//
