LOCUS       sequence-484648-        6609 bp    DNA     circular SYN 26-AUG-2025
DEFINITION  Ralstonia pseudosolanacearum filamentous phage RSCq-based cloning 
            vector carrying kanamycin resistance gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6609)
  TITLE     A filamentous phage-based versatile toolkit for molecular studies in
            Ralstonia pseudosolanacearum
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 26, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pRSCq1
FEATURES             Location/Qualifiers
     source          1..6609
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    500..518
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     primer_bind     complement(1406..1425)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1417..2232
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             1417..2232
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1490..1509)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 gtttacccct cgcccgggtt aaccggctat atttacgcca gaatcacggg cgtaattcat
       61 cacggacata atattaatta catccttcat gccgtcaagc aaagaggtgc ttagatgaga
      121 attgagaaat acttagatca ggcgatcgaa cgccacggcc tgaagaacga cagcaagctg
      181 gcagagatgc taggtgtggt gcaaagcgcg gtcagccact accgcaccgg ccgccgcacg
      241 gcggacaacg aagtgtgcct ccgcctggcg cagctgctcg agatggagaa cccgctgccg
      301 atcatcatgg cggccgacat ggaccgcgcc gaacgtgctg gccagcactc tctctgggaa
      361 gttttttcga cgaggatggc agccagtaac gcgacagccg ccctcctcct ggtactggtc
      421 gcgagcgcaa caaattttgt tgcgccctct cccgccaaag ccgcgccgtt gagccattcg
      481 acagctcaac gattattgtc tgtctcttat acacatctca accatcatcg atgaattgct
      541 tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc
      601 ggatgccatt tcattacctc tttctccgca cccgacatag atccgaagat cagcagttca
      661 acctgttgat agtacgtact aagctctcat gtttcacgta ctaagctctc atgtttaacg
      721 tactaagctc tcatgtttaa cgaactaaac cctcatggct aacgtactaa gctctcatgg
      781 ctaacgtact aagctctcat gtttcacgta ctaagctctc atgtttgaac aataaaatta
      841 atataaatca gcaacttaaa tagcctctaa ggttttaagt tttataagaa aaaaaagaat
      901 atataaggct tttaaagctt ttaaggttta acggttgtgg acaacaagcc agggatctgc
      961 catttcatta cctctttctc cgcacccgac atagatccgg aacataatgg tgcagggcgc
     1021 tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc
     1081 tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc
     1141 attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac
     1201 gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct
     1261 ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagggtg
     1321 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa
     1381 ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg
     1441 tcttgctcga ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg
     1501 gctcgcgata atgtcgggca atcaggtgcg acaatctatc gattgtatgg gaagcccgat
     1561 gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag
     1621 atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc
     1681 cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggaaaaac agcattccag
     1741 gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg
     1801 cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt
     1861 ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac
     1921 gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc
     1981 tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag
     2041 gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat
     2101 cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt
     2161 caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat
     2221 gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact
     2281 tgacgggacg gcggctttgt tgaataaatc gaacttttgc tgagttgaag gatcagatca
     2341 cgcatcttcc cgacaacgca gaccgttccg tggcaaagca aaagttcaaa atcaccaact
     2401 ggtccaccta caacaaagct ctcatcaacc gtggcgggga tcctctagag tcgacctgca
     2461 ggcatgcaag cttcagcgac gaattcagta ctcatatgga gctcggtacc gacgacgaga
     2521 ccgacgacgg tctcaacgcg ctccctccaa taaccggatc attttagacg tttcgcgtta
     2581 cgccttgcgc gtaacgactt tgcgatcacc ataaattgcc tcgccagccg cttgcatctg
     2641 cgcctcactc acggccacag aacggacaaa cgaagcgcgc cacaactcgg actcccgcac
     2701 cttctggcgc tgttcgaacg tattcgcctt cgcagccaac gcgagcaagt ccagcattgc
     2761 gtcgaacgaa acgggatttc cgttactcgg cgtaacggat ttttgctgcg actcaaacat
     2821 cgcaaccttc tcttccaaga cgccaacata acgcaacgct tcctgacgcc cgtcctcgca
     2881 cgtcataagc tgccggcgca acgcctcaat ctccgcaaga tttccgttac tcagcgtaac
     2941 ggatttggcg gcatcgctga tctcttccct cagccgccta ttctcagcaa acgccttatt
     3001 gcgttcagcc tccgccagct ccacctggcg acgcatgctc accagatcag cgcgaaactt
     3061 ctcgacctcc aggcggcatt catcgtaagc atccacgtcg gaaagcattt ccgttacggt
     3121 catacgctta gtaacggatt tagcctcaac aggctgctga gccttacggc gtgctcgata
     3181 tgcggcttga cgctccgcgt tggtcatagc gtgcgcctta cgcggacgac cgcggccacg
     3241 cctttgctcc atccccggca gatcgaccgt cacattatcc gttacgtcac gcatattcgg
     3301 ctcccgattt gatgattcca ttttacgtta ctggtaacgg caattcaatt atcgttacaa
     3361 tttagttcgt aacggaaatt tcgcgagcag ccccggcgct agacgccctc agcgggcttt
     3421 ggagccgttt tttctgactg ggcggtcgca ggggtcagct gcgccgaaat tggctcctgc
     3481 gccgcttgtg gctcgcggta ggggtcaaac ggcggcctcc tgatccactc ccgacactgc
     3541 atgtcatcga ggccggcatc cgtgccttga gccgtgtagc acgtgcacct ggtcgacgtg
     3601 caggcaccgc cgatcaccgt cggcatcgag cgaatctggc gcaactgcgc ataggccggc
     3661 gccgtctcag gccggccaga gacagcaggg acgaacgccg cgaggatctc cgccccgtcc
     3721 gtcgccgcgg cggacggacc cgccttgacc gctaccgcta gagctgaagc cacaccgccc
     3781 ctgccctgct cggccgcaac aggcttagcc ggagtcgccg tcgtcaactg gccggtacgg
     3841 taatagaccc ggtacgccaa gaacgccgcg atcaacacgc aggcgatgaa cagcatcagc
     3901 accggcggta cggtgtactt gcgcttgatg tgcagactgg aggacttgta caggccgaaa
     3961 ctggacttcg gcaggctcca cttcttcttg atcggcgcgg tgttgaacgt ctccgggttc
     4021 gcgcactccg gccattcgta gtaccagcgt ccgagcaagc cagcgtcccg caggtggaca
     4081 tgctgcccca ccagcttgcg gatatgactg tccaagaacg tcgggttctg cgtgatcaga
     4141 acaaacgtca cgccggtatg ccgcaccgtc tcaaacgccg ccacgtggtc aggcaccttg
     4201 gacccggccg tgcggacgcg aaacacccgc tgcgcctcat ccaacacgat cagcgagttc
     4261 ggcgggaagg tgaagtacgg cagcatcatc cctgggtttt cagggtcttc gcgcagctcc
     4321 gtccagtccg aaaccgccgg cgtcgggata tacggcagct tcagctccgg gatgcccatg
     4381 acgaagagcg gacgcccctg gtcgacggct gccttcatca tctggaccgc caacgctgtc
     4441 ttcccgccac caggcgtggc cgtgatcagc gtgatcggtt gcgttgcgcg aggaacctaa
     4501 tccgttacgt cacgctaggc agaaccggca gcaggaatcc ggcccagagc cagaaatcga
     4561 tcgcgagcat cacagcccct ttttcatcac caccagcgcc caggccgcca ccatcgcggc
     4621 aacgacgccc caccccatcg tcatgccatc cttgaagctc tcttgcgggt cacatgcagg
     4681 aaacgacagc gacggcaacg acgcatcggt caacgtgccg acgctcccat cactgcccac
     4741 ctgataccgc cgcagcaccc acccgcccgt ggtcttgacg aactcagaca gataggtgac
     4801 tgccccaggt gtctgcgacg gagcgacggc gctgtaatag gcatccgtgg ccatgcccac
     4861 atcggcaaag caccgtgcgc ccaccaacgc gccgtcagca gccaatcggc aaagcaccgt
     4921 gcgcggtttt ctccttgaga tggaggttga gaaaagctcc gggccgttca actcccagag
     4981 ccagggaaca tcacgccttc ttgggctcag cctcagcacg ctgcagcggc ttgatgctcg
     5041 taacgacctt ctgaccgccc ttatccttgc cgttgctcgt ctcgaccatc gagacttccg
     5101 cgatgaacgg gaacgggttg tggatgatcg ccttcacaac cgcagacgac tcgcacttca
     5161 gctcctgcgt gcaggtgccc ttcgagtctt cgccacgcag ctccacatcc gtgtagatct
     5221 tcccggtgtc cagctgcttg ccatccatgt tgccaaccca cgtcttggcg ccccggatgg
     5281 tcacgcgtgc aatcatttcc atggtttcac tcctcaggtt tcggcactgg atcgtgcgtg
     5341 ccgtacacgt gcctcgccag ggcggatttg tgcagctgag ccggtacgcc ctgacggcgt
     5401 atcgcgacaa ccagcgcagc gatgtcttcc tctgtgcagc gcagctcgta atcgaccgta
     5461 gggccgaact gcgtctggat gtgcttgagc ttgcgttcgc ggatggtttc gtcctgaagc
     5521 tccaaggcct tgacctggtc agtaggaacg cgctgcggat ccgcagccat gaaggcctcc
     5581 agggccttgt acgcaccagc gaaatactgg tcgcgcttga taaggatttc gtgcggaatc
     5641 acgcgatcct tggcgccgaa ctcgatctcg agacgcaccc actcgctatc ctgattgccg
     5701 agctggcggc ccttctcgta agcccgcagc atcttgccgt tcgcccgacg gccaatctcg
     5761 aacgtcgtac cgcgacagcc cttgctgccc gccacgccgc tctcgatctt gcgatacgtc
     5821 gggatacgcc cgcccgcgtt gaagtcgccg gcgtagtaca gctcttccat ctgcgcgatg
     5881 ctcacctcgc cctggcagaa gtccatcgcc aggtcgcacc gcgtgatccg cgcgtcgagg
     5941 tcctgcacca tcgcgtagac ggcttgccag tcgccaatcg cggtgcagcc ctgccccggc
     6001 cagtccacca ggatcgtgcc gccaacgtgc tcgccgccgc aggcaacgat cccgagcttc
     6061 atcgtctcgc cgttgatgaa cgccagcagg tcgtagctga actcataccg gcggaaccct
     6121 ttgcccgcag gcttcatcgt caccggcacc gagaacacca gctggaagta cctgcgcagc
     6181 tgctccaggg cgtcgctgat gctgccgtcg ggcaggaacg tgaacttgaa ccagtccacg
     6241 atcgcacctg ccttgcgttc tggactttcc ccgggtttta ccggacccgg ggaacggcct
     6301 gtcggccgcc atgccgttct cgcgtgcgag cgccggccca tgaactccat acgcaatgcc
     6361 tgtgggatca agatgacgag agggggtggg acggacgcgc atcagcgtgc gccctcgcag
     6421 cgataccagt tcgccagatc ggcctggcgc gagttcttca ggaggtcgtc aatgcgatgc
     6481 atcgcaggac gcgattgcag gcagcaggcg ccgcgcagaa cggcagccat cacgatggcc
     6541 agctgcgcac cgtgcgggtg aagcacgtcg ccaatcaggt ccagctgcct atgatctctc
     6601 tgctgcatc
//
