LOCUS       sequence-484649-        6791 bp    DNA     circular SYN 26-AUG-2025
DEFINITION  Ralstonia pseudosolanacearum filamentous phage RSCq-based cloning 
            vector carrying ampicillin resistance gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6791)
  TITLE     A filamentous phage-based versatile toolkit for molecular studies in
            Ralstonia pseudosolanacearum
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 26, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pRSCq3
FEATURES             Location/Qualifiers
     source          1..6791
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    501..519
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     CDS             complement(join(1418..2209,2210..2278))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2041..2060
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2279..2383)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgtttacccc tcgcccgggt taaccggcta tatttacgcc agaatcacgg gcgtaattca
       61 tcacggacat aatattaatt acatccttca tgccgccaag caaagaggtg cttagatgag
      121 aattgagaaa tacttagatc aggcgatcga acgccacggc ctgaagaacg acagcaagct
      181 ggcagagatg ctaggtgtgg tgcaaagcgc ggtcagccac taccgcaccg gccgccgcac
      241 ggcggacaac gaagtgtgcc tccgcctggc gcagctgctc gagatggaga acccgctgcc
      301 gatcatcatg gcggccgaca tggaccgcgc cgaacgtgct ggccagcact ctctctggga
      361 agttttttcg acgaggatgg cagccagtaa cgcgacagcc gccctcctcc tggtactggt
      421 cgcgagcgca acaaattttg ttgcgccctc tcccgccaaa gccgcgccgt tgagccattc
      481 gacagctcaa cgattattgt ctgtctctta tacacatctc aaccatcatc gatgaattgc
      541 ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc
      601 cggatgccat ttcattacct ctttctccgc acccgacata gatccgaaga tcagcagttc
      661 aacctgttga tagtacgtac taagctctca tgtttcacgt actaagctct catgtttaac
      721 gtactaagct ctcatgttta acgaactaaa ccctcatggc taacgtacta agctctcatg
      781 gctaacgtac taagctctca tgtttcacgt actaagctct catgtttgaa caataaaatt
      841 aatataaatc agcaacttaa atagcctcta aggttttaag ttttataaga aaaaaaagaa
      901 tatataaggc ttttaaagct tttaaggttt aacggttgtg gacaacaagc cagggatctg
      961 ccatttcatt acctctttct ccgcacccga catagatccg gaacataatg gtgcagggcg
     1021 ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg
     1081 ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt
     1141 cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca
     1201 cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc
     1261 tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagggt
     1321 gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa
     1381 actgtctgct tacataaaca gtaatacaag gggtgtttta ccaatgctta atcagtgagg
     1441 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     1501 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgtg
     1561 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     1621 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     1681 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     1741 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     1801 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     1861 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     1921 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     1981 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     2041 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     2101 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     2161 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     2221 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     2281 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     2341 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgtcagaat tggttaattg
     2401 gttgtaacac tggcagagca ttacgctgac ttgacgggac ggcggctttg ttgaataaat
     2461 cgaacttttg ctgagttgaa ggatcagatc acgcatcttc ccgacaacgc agaccgttcc
     2521 gtggcaaagc aaaagttcaa aatcaccaac tggtccacct acaacaaagc tctcatcaac
     2581 cgtggcgggg atcctctaga gtcgacctgc aggcatgcaa gcttcagcga cgaattcagt
     2641 actcatatgg agctcggtac cgacgacgag accgacgacg gtctcaacgc gctccctcca
     2701 ataaccggat cattttagac gtttcgcgtt acgccttgcg cgtaacgact ttgcgatcac
     2761 cataaattgc ctcgccagcc gcttgcatct gcgcctcact cacggccaca gaacggacaa
     2821 acgaagcgcg ccacaactcg gactcccgca ccttctggcg ctgttcgaac gtattcgcct
     2881 tcgcagccaa cgcgagcaag tccagcattg cgtcgaacga aacgggattt ccgttactcg
     2941 gcgtaacgga tttttgctgc gactcaaaca tcgcaacctt ctcttccaag acgccaacat
     3001 aacgcaacgc ttcctgacgc ccgtcctcgc acgtcataag ctgccggcgc aacgcctcaa
     3061 tctccgcaag atttccgtta ctcagcgtaa cggatttggc ggcatcgctg atctcttccc
     3121 tcagccgcct attctcagca aacgccttat tgcgttcagc ctccgccagc tccacctggc
     3181 gacgcatgct caccagatca gcgcgaaact tctcgacctc caggcggcat tcatcgtaag
     3241 catccacgtc ggaaagcatt tccgttacgg tcatacgctt agtaacggat ttagcctcaa
     3301 caggctgctg agccttacgg cgtgctcgat atgcggcttg acgctccgcg ttggtcatag
     3361 cgtgcgcctt acgcggacga ccgcggccac gcctttgctc catccccggc agatcgaccg
     3421 tcacattatc cgttacgtca cgcatattcg gctcccgatt tgatgattcc attttacgtt
     3481 actggtaacg gcaattcaat tatcgttaca atttagttcg taacggaaat ttcgcgagca
     3541 gccccggcgc tagacgccct cagcgggctt tggagccgtt ttttctgact gggcggtcgc
     3601 aggggtcagc tgcgccgaaa ttggctcctg cgccgcttgt ggctcgcggt aggggtcaaa
     3661 cggcggcctc ctgatccact cccgacactg catgtcatcg aggccggcat ccgtgccttg
     3721 agccgtgtag cacgtgcacc tggtcgacgt gcaggcaccg ccgatcaccg tcggcatcga
     3781 gcgaatctgg cgcaactgcg cataggccgg cgccgtctca ggccggccag agacagcagg
     3841 gacgaacgcc gcgaggatct ccgccccgtc cgtcgccgcg gcggacggac ccgccttgac
     3901 cgctaccgct agagctgaag ccacaccgcc cctgccctgc tcggccgcaa caggcttagc
     3961 cggagtcgcc gtcgtcaact ggccggtacg gtaatagacc cggtacgcca agaacgccgc
     4021 gatcaacacg caggcgatga acagcatcag caccggcggt acggtgtact tgcgcttgat
     4081 gtgcagactg gaggacttgt acaggccgaa actggacttc ggcaggctcc acttcttctt
     4141 gatcggcgcg gtgttgaacg tctccgggtt cgcgcactcc ggccattcgt agtaccagcg
     4201 tccgagcaag ccagcgtccc gcaggtggac atgctgcccc accagcttgc ggatatgact
     4261 gtccaagaac gtcgggttct gcgtgatcag aacaaacgtc acgccggtat gccgcaccgt
     4321 ctcaaacgcc gccacgtggt caggcacctt ggacccggcc gtgcggacgc gaaacacccg
     4381 ctgcgcctca tccaacacga tcagcgagtt cggcgggaag gtgaagtacg gcagcatcat
     4441 ccctgggttt tcagggtctt cgcgcagctc cgtccagtcc gaaaccgccg gcgtcgggat
     4501 atacggcagc ttcagctccg ggatgcccat gacgaagagc ggacgcccct ggtcgacggc
     4561 tgccttcatc atctggaccg ccaacgctgt cttcccgcca ccaggcgtgg ccgtgatcag
     4621 cgtgatcggt tgcgttgcgc gaggaaccta atccgttacg tcacgctagg cagaaccggc
     4681 agcaggaatc cggcccagag ccagaaatcg atcgcgagca tcacagcccc tttttcatca
     4741 ccaccagcgc ccaggccgcc accatcgcgg caacgacgcc ccaccccatc gtcatgccat
     4801 ccttgaagct ctcttgcggg tcacatgcag gaaacgacag cgacggcaac gacgcatcgg
     4861 tcaacgtgcc gacgctccca tcactgccca cctgataccg ccgcagcacc cacccgcccg
     4921 tggtcttgac gaactcagac agataggtga ctgccccagg tgtctgcgac ggagcgacgg
     4981 cgctgtaata ggcatccgtg gccatgccca catcggcaaa gcaccgtgcg cccaccaacg
     5041 cgccgtcagc agccaatcgg caaagcaccg tgcgcggttt tctccttgag atggaggttg
     5101 agaaaagctc cgggccgttc aactcccaga gccagggaac atcacgcctt cttgggctca
     5161 gcctcagcac gctgcagcgg cttgatgctc gtaacgacct tctgaccgcc cttatccttg
     5221 ccgttgctcg tctcgaccat cgagacttcc gcgatgaacg ggaacgggtt gtggatgatc
     5281 gccttcacaa ccgcagacga ctcgcacttc agctcctgcg tgcaggtgcc cttcgagtct
     5341 tcgccacgca gctccacatc cgtgtagatc ttcccggtgt ccagctgctt gccatccatg
     5401 ttgccaaccc acgtcttggc gccccggatg gtcacgcgtg caatcatttc catggtttca
     5461 ctcctcaggt ttcggcactg gatcgtgcgt gccgtacacg tgcctcgcca gggcggattt
     5521 gtgcagctga gccggtacgc cctgacggcg tatcgcgaca accagcgcag cgatgtcttc
     5581 ctctgtgcag cgcagctcgt aatcgaccgt agggccgaac tgcgtctgga tgtgcttgag
     5641 cttgcgttcg cggatggttt cgtcctgaag ctccaaggcc ttgacctggt cagtaggaac
     5701 gcgctgcgga tccgcagcca tgaaggcctc cagggccttg tacgcaccag cgaaatactg
     5761 gtcgcgcttg ataaggattt cgtgcggaat cacgcgatcc ttggcgccga actcgatctc
     5821 gagacgcacc cactcgctat cctgattgcc gagctggcgg cccttctcgt aagcccgcag
     5881 catcttgccg ttcgcccgac ggccaatctc gaacgtcgta ccgcgacagc ccttgctgcc
     5941 cgccacgccg ctctcgatct tgcgatacgt cgggatacgc ccgcccgcgt tgaagtcgcc
     6001 ggcgtagtac agctcttcca tctgcgcgat gctcacctcg ccctggcaga agtccatcgc
     6061 caggtcgcac cgcgtgatcc gcgcgtcgag gtcctgcacc atcgcgtaga cggcttgcca
     6121 gtcgccaatc gcggtgcagc cctgccccgg ccagtccacc aggatcgtgc cgccaacgtg
     6181 ctcgccgccg caggcaacga tcccgagctt catcgtctcg ccgttgatga acgccagcag
     6241 gtcgtagctg aactcatacc ggcggaaccc tttgcccgca ggcttcatcg tcaccggcac
     6301 cgagaacacc agctggaagt acctgcgcag ctgctccagg gcgtcgctga tgctgccgtc
     6361 gggcaggaac gtgaacttga accagtccac gatcgcacct gccttgcgtt ctggactttc
     6421 cccgggtttt accggacccg gggaacggcc tgtcggccgc ccagcctcgc tccgctcggc
     6481 ccggtcggcc gccatgccgt tctcgcgtgc gagcgccggc ccatgaactc catacgcaat
     6541 gcctgtggga tcaagatgac gagagggggt gggacggacg cgcatcagcg tgcgccctcg
     6601 cagcgatacc agttcgccag atcggcctgg cgcgagttct tcaggaggtc gtcaatgcga
     6661 tgcatcgcag gacgcgattg caggcagcag gcgccgcgca gaacggcagc catcacgatg
     6721 gccagctgcg caccgtgcgg gtgaagcacg tcgccaatca ggtccagctg cctatgatct
     6781 ctctgctgca t
//
