LOCUS       sequence-484651-        4655 bp    DNA     circular SYN 26-AUG-2025
DEFINITION  Ralstonia pseudosolanacearum filamentous phage RSCq-based cloning 
            vector carrying spectinomycin resistance gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4655)
  TITLE     A filamentous phage-based versatile toolkit for molecular studies in
            Ralstonia pseudosolanacearum
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 26, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pRSCq2
FEATURES             Location/Qualifiers
     source          1..4655
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    501..519
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     CDS             1636..2427
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cgtttacccc tcgcccgggt taaccggcta tatttacgcc agaatcacgg gcgtaattca
       61 tcacggacat aatattaatt acatccttca tgccgtcaag caaagaggtg cttagatgag
      121 aattgagaaa tacttagatc aggcgatcga acgccacggc ctgaagaacg acagcaagct
      181 ggcagagatg ctaggtgtgg tgcaaagcgc ggtcagccac taccgcaccg gccgccgcac
      241 ggcggacaac gaagtgtgcc tccgcctggc gcagctgctc gagatggaga acccgctgcc
      301 gatcatcatg gcggccgaca tggaccgcgc cgaacgtgct ggccagcact ctctctggga
      361 agttttttcg acgaggatgg cagccagtaa cgcgacagcc gccctcctcc tggtactggt
      421 cgcgagcgca acaaattttg ttgcgccctc tcccgccaaa gccgcgccgt tgagccattc
      481 gacagctcaa cgattattgt ctgtctctta tacacatctc aaccatcatc gatgaattgc
      541 ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc
      601 cggatgccat ttcattacct ctttctccgc acccgacata gatccgaaga tcagcagttc
      661 aacctgttga tagtacgtac taagctctca tgtttcacgt actaagctct catgtttaac
      721 gtactaagct ctcatgttta acgaactaaa ccctcatggc taacgtacta agctctcatg
      781 gctaacgtac taagctctca tgtttcacgt actaagctct catgtttgaa caataaaatt
      841 aatataaatc agcaacttaa atagcctcta aggttttaag ttttataaga aaaaaaagaa
      901 tatataaggc ttttaaagct tttaaggttt aacggttgtg gacaacaagc cagggatctg
      961 ccatttcatt acctctttct ccgcacccga catagatccg gaacataatg gtgcagggcg
     1021 ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg
     1081 ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt
     1141 cattctgcta accagtaagg caccccgcca gcctagccgg gtcctcaacg acaggagcac
     1201 gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct
     1261 ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagggtg
     1321 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa
     1381 ctgtctgctt acataaacag taatacaagg ggtgttatgc gctcacgcaa ctggtccaga
     1441 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1501 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     1561 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     1621 acaaagttaa acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     1681 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     1741 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     1801 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     1861 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     1921 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     1981 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2041 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2101 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2161 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2221 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2281 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2341 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2401 gagatcacca aggtagtcgg caaataatca gaattggtta attggttgta acactggcag
     2461 agcattacgc tgacttgacg ggacggcggc tttgttgaat aaatcgaact tttgctgagt
     2521 tgaaggatca gatcacgcat cttcccgaca acgcagaccg ttccgtggca aagcaaaagt
     2581 tcaaaatcac caactggtcc acctacaaca aagctctcat caaccgtggc ggggatcctc
     2641 tagagtcgac ctgcaggcat gcaagcttca gcgacgaatt cagtactcat atggagctcg
     2701 gtaccgacga cgagaccgac gacggtctca acgcgctccc tccaataacc ggatcatttt
     2761 agacgtttcg cgttacgcct tgcgcgtaac gactttgcga tcaccataaa ttgcctcgcc
     2821 agccgcttgc atctgcgcct cactcacggc cacagaacgg acaaacgaag cgcgccacaa
     2881 ctcggactcc cgcaccttct ggcgctgttc gaacgtattc gccttcgcag ccaacgcgag
     2941 caagtccagc attgcgtcga acgaaacggg atttccgtta ctcggcgtaa cggatttttg
     3001 ctgcgactca aacatcgcaa ccttctcttc caagacgcca acataacgca acgcttcctg
     3061 acgcccgtcc tcgcacgtca taagctgccg gcgcaacgcc tcaatctccg caagatttcc
     3121 gttactcagc gtaacggatt tggcggcatc gctgatctct tccctcagcc gcctattctc
     3181 agcaaacgcc ttattgcgtt cagcctccgc cagctccacc tggcgacgca tgctcaccag
     3241 atcagcgcga aacttctcga cctccaggcg gcattcatcg taagcatcca cgtcggaaag
     3301 catttccgtt acggtcatac gcttagtaac ggatttagcc tcaacaggct gctgagcctt
     3361 acggcgtgct cgatatgcgg cttgacgctc cgcgttggtc atagcgtgcg ccttacgcgg
     3421 acgaccgcgg ccacgccttt gctccatccc cggcagatcg accgtcacat tatccgttac
     3481 gtcacgcata ttcggctccc gatttgatga ttccatttta cgttactggt aacggcaatt
     3541 caattatcgt tacaatttag ttcgtaacgg aaatttcgcg agcagccccg gcgctagacg
     3601 ccctcagcgg gctttggagc cgttttttct gactgggcgg tcgcaggggt cagctgcgcc
     3661 gaaattggct cctgcgccgc ttgtggctcg cggtaggggt caaacggcgg cctcctgatc
     3721 cactcccgac actgcatgtc atcgaggccg gcatccgtgc cttgagccgt gtagcacgtg
     3781 cacctggtcg acgtgcaggc accgccgatc accgtcggca tcgagcgaat ctggcgcaac
     3841 tgcgcatagg ccggcgccgt ctcaggccga gtacagctct tccatctgcg cgatgctcac
     3901 ctcgccctgg cagaagtcca tcgccaggtc gcaccgcgtg atccgcgcgt cgaggtcctg
     3961 caccatcgcg tagacggctt gccagtcgcc aatcgcggtg cagccctgcc ccggccagtc
     4021 caccaggatc gtgccgccaa cgtgctcgcc gccgcaggca acgatcccga gcttcatcgt
     4081 ctcgccgttg atgaacgcca gcaggtcgta gctgaactca taccggcgga accctttgcc
     4141 cgcaggcttc atcgtcaccg gcaccgagaa caccagctgg aagtacctgc gcagctgctc
     4201 cagggcgtcg ctgatgctgc cgtcgggcag gaacgtgaac ttgaaccagt ccacgatcgc
     4261 acctgccttg cgttctggac tttccccggg ttttaccgga cccggggaac ggcctgtcgg
     4321 ccgcccagcc tcgctccgct cggcccggtc ggccgccatg ccgttctcgc gtgcgagcgc
     4381 cggcccatga actccatacg caatgcctgt gggatcaaga tgacgagagg gggtgggacg
     4441 gacgcgcatc agcgtgcgcc ctcgcagcga taccagttcg ccagatcggc ctggcgcgag
     4501 ttcttcagga ggtcgtcaat gcgatgcatc gcaggacgcg attgcaggca gcaggcgccg
     4561 cgcagaacgg cagccatcac gatggccagc tgcgcaccgt gcgggtgaag cacgtcgcca
     4621 atcaggtcca gctgcctatg atctctctgc tgcat
//
