LOCUS       sequence-484676-       10158 bp    DNA     circular SYN 26-AUG-2025
DEFINITION  Dual sgRNA targeting TIMM23 expressing dAPEX-BFP targeting to ER.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10158)
  AUTHORS   Thayer JA, Petersen JD, Huang X, Hawrot J, Ramos DM, Sekine S, Li Y,
            Ward ME, Narendra DP
  TITLE     Novel reporter of the PINK1-Parkin mitophagy pathway identifies its 
            damage sensor in the import gate.
  JOURNAL   bioRxiv [Preprint]. 2025 Feb 20:2025.02.19.639160. doi: 
            10.1101/2025.02.19.639160.
  PUBMED    40027798
REFERENCE   2  (bases 1 to 10158)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 26, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2025 Feb 20:2025.02.19.639160. doi: 
            10.1101/2025.02.19.639160."
COMMENT     Sequence Label: pLenti_DualsgRNA_TIMM23_ER-dAPEX-BFP2
FEATURES             Location/Qualifiers
     source          1..10158
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(207..227)
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     protein_bind    231..264
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    517..634
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(932..976)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1161..1394
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1891..2016
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             2063..2243
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(2261..2459)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2286..2306)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2461..2840
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     3012..3031
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        3106..3210
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3211..3279,3280..4071)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3429..3448)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4242..4830
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4731..4750
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             4892..5072
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     5581..5597
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     5582..5598
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    5601..6189
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5707..5718
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6116..6136
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    6245..6278
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(6313..7023)
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             complement(7078..7824)
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQKAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPEVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     sig_peptide     complement(7825..7887)
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTGD"
     regulatory      7884..7893
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(7900..9078)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          7909..8847
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(7932..7952)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     complement(9950..9969)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(10120..10140)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 gcgcgccata gctgagtgta gattcgagca aaaaaagcac ccgactcggg tgccactttt
       61 tcaagttgta aacggactag ccttatttca acttgctatg cactcttgtg cttagctctg
      121 aaacctgggc cgttactcct caaccaacaa ggtggttctc caagggatac ttatagtctc
      181 aaaacacaca attactttac agttagggtg agtttccttt tgtgctgttt ataacttcgt
      241 ataatgtatg ctatacgaag ttatagaaat ccaagcctat catgtaaaat gtagctagta
      301 ttaaaaagaa cagattatct gtcttttatc gcacattaag cctctatagt tactaggaaa
      361 tattatatgc aaattaaccg gggcagggga gtagccgagc ttctcccaca agtccgtgcg
      421 agggggccgg cgcgggccta gagatggcgc gtcggatctc tagagcgggc ccggtactaa
      481 ccaaactgga tctctgctgt ccctgtaata aacccgaaaa ttttgaattt ttgtaatttg
      541 tttttgtaat tctttagttt gtatgtctgt tgctattatg tctactattc tttcccctgc
      601 actgtacccc ccaatccccc cttttctttt aaaattgtgg atgaatactg ccatttgtct
      661 gcagaattgg cgcacgcagt gccgatccgt tcactaatcg aatggatctg tctctgtctc
      721 tctctccacc ttcttcttct attccttcgg gcctgtcggg tcccctcggg gttgggaggt
      781 gggtctgaaa cgataatggt gaatatccct gcctaactct attcactata gaaagtacag
      841 caaaaactat tcttaaacct accaagcctc ctactatcat tatgaataat tttatatacc
      901 acagccaatt tgttatgtta aaccaattcc acaaacttgc ccatttatct aattccaata
      961 attcttgttc attcttttct tgctggtttt gcgattcttc aattaaggag tgtattaagc
     1021 ttgtgtaatt gttaatttct ctgtcccact ccatccaggt cgtgtgattc caaatctgtt
     1081 ccagagattt attactccaa ctagcattcc aaggcacagc agtggtgcaa atgagttttc
     1141 cagagcaacc ccaaatcccc aggagctgtt gatcctttag gtatctttcc acagccagga
     1201 ttcttgcctg gagctgcttg atgccccaga ctgtgagttg caacagatgc tgttgcgcct
     1261 caatagccct cagcaaattg ttctgctgct gcactatacc agacaataat tgtctggcct
     1321 gtaccgtcag cgtcattgac gctgcgccca tagtgcttcc tgctgctccc aagaacccaa
     1381 ggaacaaagc tcctattccc actgctcttt tttctctctg caccactctt ctctttgcct
     1441 tggtgggtgc tactcctaat ggttcaattt ttactacttt atatttatat aattcacttc
     1501 tccaattgtc cctcatatct cctcctccag gtctgaagat cagcggccgg ccgcttgctg
     1561 tgcggtggtc ttacttttgt tttgctcttc ctctatcttg tctaaagctt ccttggtgtc
     1621 ttttatctct atcctttgat gcacacaata gagggttgct actgtattat ataatgatct
     1681 aagttcttct gatcctgtct gaagggatgg ttgtagctgt cccagtattt gtctacagcc
     1741 ttctgatgtt tctaacaggc caggattaac tgcgaatcgt tctagctccc tgcttgccca
     1801 tactatatgt tttaatttat attttttctt tccccctggc cttaaccgaa ttttttccca
     1861 tcgcgatcta attctccccc gcttaatact gacgctctcg cacccatctc tctccttcta
     1921 gcctccgcta gtcaaaattt ttggcgtact caccagtcgc cgcccctcgc ctcttgccgt
     1981 gcgcgcttca gcaagccgag tcctgcgtcg agagagctcc tctggtttcc ctttcgcttt
     2041 caagtccctg ttcgggcgcc actgctagag attttccaca ctgactaaaa gggtctgagg
     2101 gatctctagt taccagagtc acacaacaga cgggcacaca ctacttgaag cactcaaggc
     2161 aagctttatt gaggcttaag cagtgggttc cctagttagc cagagagctc ccaggctcag
     2221 atctggtcta accagagaga cccagtacag gcaaaacgcg ctgcttatat agacctccca
     2281 ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag ttgttacgac attttggaaa
     2341 gtcccgttga ttttggtgcc aaaacaaact cccattgacg tcaatggggt ggagacttgg
     2401 aaatccccgt gagtcaaacc gctatccacg cccattgatg tactgccaaa accgcatcac
     2461 catggtaata gcgatgacta atacgtagat gtactgccaa gtaggaaagt cccataaggt
     2521 catgtactgg gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt
     2581 acttggcata tgatacactt gatgtactgc caagtgggca gtttaccgta aatactccac
     2641 ccattgacgt caatggaaag tccctattgg cgttactatg ggaacatacg tcattattga
     2701 cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg ccatttaccg taagttatgt
     2761 aacgcggaac tccatatatg ggctatgaac taatgacccc gtaattgatt actattaata
     2821 actagtcaat aatcaatgtc aacgcgtata tctggcccgt acatcgcgaa gcagcgcaaa
     2881 acgcctaacc ctaagcagat tcttcatgca attgtcggtc aagccttgcc ttgttgtagc
     2941 ttaaattttg ctcgcgcact actcagcgac ctccaacaca caagcaggga gcagatactg
     3001 gcttaactat gcggcatcag agcagattgt actgagagtg caccataggg gatcgggaga
     3061 tctcccgatc cgtcgacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     3121 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     3181 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     3241 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     3301 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     3361 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     3421 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     3481 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     3541 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     3601 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     3661 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     3721 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     3781 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     3841 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     3901 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     3961 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     4021 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     4081 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     4141 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     4201 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     4261 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     4321 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     4381 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     4441 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     4501 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     4561 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     4621 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     4681 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     4741 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     4801 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     4861 ctggcctttt gctggccttt tgctcacatg ctgctagaga ttttccacac tgactaaaag
     4921 ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc
     4981 actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc
     5041 caggctcaga tctggtctaa ccagagagac ccagtacagt ccggatgcag ctctcgggcc
     5101 atgtgatgaa atgctaggcg gctgtcaaac ctccactcta atacttctct ctccgggtca
     5161 tccatcccat gcaggctcac agggtgtaac aagcgggtgt tctctccttc attggcttct
     5221 tctaccttct cttgctcaac tggtactagc ttgtagcacc atccaaaggt cagtggatat
     5281 ctgatccctg gccctggtgt gtagttctgc caatcaggga agtagccttg tgtgtggtag
     5341 atccacagat caaggatatc ttgtcttcgt tgggagtgaa ttagcccttc cagtcccccc
     5401 ttttctttta aaaagtggct aagatctaca gctgccttgt aagtcattgg tcttaaaggt
     5461 acctgaggtg tgactggaaa acccacctcc tcctcctctt gtgcttctag ccaggcacaa
     5521 tcagcattgg tagctgctgt attgctactt gtgattgctc catgtttttc taggtctcga
     5581 tcgaggtcga cggtatcgat gcggggaggc ggcccaaagg gagatccgac tcgtctgagg
     5641 gcgaaggcga agacgcggaa gaggccgcag agccggcagc aggccgcggg aaggaaggtc
     5701 cgctggattg agggccgaag ggacgtagca gaaggacgtc ccgcgcagaa tccaggtggc
     5761 aacacaggcg agcagccaag gaaaggacga tgatttcccc gacaacacca cggaattgtc
     5821 agtgcccaac agccgagccc ctgtccagca gcgggcaagg caggcggcga tgagttccgc
     5881 cgtggcaata gggaggggga aagcgaaagt cccggaaagg agctgacagg tggtggcaat
     5941 gccccaacca gtgggggttg cgtcagcaaa cacagtgcac accacgccac gttgcctgac
     6001 aacgggccac aactcctcat aaagagacag caaccaggat ttatacaagg aggagaaaat
     6061 gaaagccata cgggaagcaa tagcatgata caaaggcatt aaagcagcgt atccacatag
     6121 cgtaaaagga gcaacatagt taagaatacc agtcaatctt tcacaaattt tgtaatccag
     6181 aggttgatta tcgataagct tgatatcgaa ttgtacctag tggaaccgga acccttaaac
     6241 atgtataact tcgtataatg tatgctatac gaagttatta ggtccctcga cgaattctta
     6301 cagctcgtcc ttattaagct tgtgccccag tttgctaggg aggtcgcagt atctggccac
     6361 tgccacctcg tgctgctcga cgtaggtctc gttgttggcc tccttgattc tttccagtct
     6421 gtagtccaca tagtagacgc caggcatctt gaggttctta gcgggtttct tggatctata
     6481 tgtggtcttg gcgtttgcga tcagatggct cccgcccacg agcttcaggg ccatgtcgtt
     6541 tctgccttcc aggccgccgt cagcggggta cagcgtctcg gtgaaggcct cccagccgag
     6601 tgttttcttc tgcatcacag ggccgttgga tgtgaagttc acccctctga tcttgacgtt
     6661 gtagatgagg cagccgtcct ggaggctggt gtcctgggta gcggtcagca cgcccccgtc
     6721 ttcgtatgtg gtgactctct cccatgtgaa gccctcaggg aaggactgct tgaagaagtc
     6781 ggggatgccc tgggtgtggt tgatgaaggt cttgctgccg tagaggaagc tagtagccag
     6841 gatgtcgaag gcgaagggga gagggccgcc ctcgaccacc ttgattctca tggtctgggt
     6901 gccctcgtag ggcttgcctt cgccctcgga tgtgcacttg aagtgatggt tgtccacggt
     6961 gccctccatg tacagcttca tgtgcatgtt ctccttaatc agctcttcgc ccttagacac
     7021 catgccaccg ccaccactac caccaccacc actgctgcta ctacggctgc taccgccggc
     7081 atcagcaaac ccaagctcgg aaagcttttg gtgagcctca gcgtaatcag caaagaaggc
     7141 atcttcgtcc gctgcatatt tatcaacgag agggcggaat acagggtcag acaaaagagc
     7201 cttgtcagaa ggtagctgaa ggagaccttc cttctcacca ctcaacaact ccgtgaagta
     7261 tgagttgtcg aaaataagag gattagaggt ccagggaccc tcaaatccag aacgctcctt
     7321 gtgtgcagct ccaatagtgt gacccccaga tagagcaacg atatcttggt cagtaagccc
     7381 catagctttg ccaaacacat ctctcaaatg gtcagaaccc ttagtgggat cgggcaagcg
     7441 accctctggt ggtggctcag gcttgtcctc tcttccaggg tggaatggaa cttcaggtcc
     7501 acccgtgacc tcaacggcaa caacgccagc caactggtag aaatcggcgt agctcaaaat
     7561 agggaactcc gccttgagtg gctccaaaag cctaacagcg atgtcaagac cgttgttagc
     7621 gctgtgagcc agttcggcag ggtgcttgat ggttccgaag ggtccaccgg tcttcgtgcc
     7681 cttgtcaaag gttccagcag agtggaatgc caaacggagc attagaggag cgcatctctt
     7741 ctcagcgatg aagcctctga gcttcttctt cgccttctca acggccttct ggtaatcagc
     7801 actcacagtt gggtaagact ttccgtcacc agtggaacct ggaacccaga gcagcagtac
     7861 ccatagcagg agtgtgtctg tctccatggt ggcgctagct cacgacacct gaaatggaag
     7921 aaaaaaactt tgaaccactg tctgaggctt gagaatgaac caagatccaa actcaaaaag
     7981 ggcaaattcc aaggagaatt acatcaagtg ccaagctggc ctaacttcag tctccaccca
     8041 ctcagtgtgg ggaaactcca tcgcataaaa cccctccccc caacctaaag acgacgtact
     8101 ccaaaagcgc gagaactaat cgaggtgcct ggacggcgcc cggtactccg tggagtcaca
     8161 tgaagcgacg gctgaggacg gaaaggccct tttcctttgt gtgggtgact cacccgcccg
     8221 ctctcccgag cgccgcgtcc tccattttga gctccctgca gcagggccgg gaagcggcca
     8281 tctttccgct cacgcaactg gtgccgaccg ggccagcctt gccgcccagg gcggggcgat
     8341 acacggcggc gcgaggccag gcaccagagc aggccggcca gcttgagact acccccgtcc
     8401 gattctcggt ggccgcgctc gcaggccccg cctcgccgaa catgtgcgct gggacgcacg
     8461 ggccccgtcg ccgcccgcgg ccccaaaaac cgaaatacca gtgtgcagat cttggcccgc
     8521 atttacaaga ctatcttgcc agaaaaaaag cgtcgcagca ggtcatcaaa aattttaaat
     8581 ggctagagac ttatcgaaag cagcgagaca ggcgcgaagg tgccaccaga ttcgcacgcg
     8641 gcggccccag cgcccaggcc aggcctcaac tcaagcacga ggcgaagggg ctccttaagc
     8701 gcaaggcctc gaactctccc acccacttcc aacccgaagc tcgggatcaa gaatcacgta
     8761 ctgcagccag gtggaagtaa ttcaaggcac gcaagggcca taacccgtaa agaggccagg
     8821 cccgcgggaa ccacacacgg cacttacctg tgttctggcg gcaaacccgt tgcgaaaaag
     8881 aacgttcacg gcgactactg cacttatata cggttctccc ccaccctcgg gaaaaaggcg
     8941 gagccagtac acgacatcac tttcccagtt taccccgcgc caccttctct aggcaccggt
     9001 tcaattgccg acccctcccc ccaacttctc ggggactgtg ggcgatgtgc gctctgccca
     9061 ctgacgggca catgcatctc cgcgcctaca gctcaagcca catccgaagg gggagggagc
     9121 cgggagctgc gcgcggggcc gccgggggga ggggtggcac cgcccacgcc gggcggccac
     9181 gaagggcggg gcagcgggcg cgcgcccggc ggggggaggg gccgcgcgcc gcgcccgctg
     9241 ggaattgggg ccctaggggg agggcggagg cgccgacgac cgcggcactt accgttcgcg
     9301 gcgtggcgcc cggtggtccc caaggggagg gaagggggag gcggggcgag gacagtgacc
     9361 ggagtctcct cagcggtggc ttttctgctt ggcagcctca gcggctggcg ccaaaaccgg
     9421 actccgccca cttcctcgcc cctgcggtgc gagggtgtgg aatcctccag acgctggggg
     9481 agggggagtt gggagcttaa aaactagtac ccctttggga ccactttcag cagcgaactc
     9541 tcctgtacac caggggtcag ttccacagac gcgggccagg ggtgggtcat tgcggcgtga
     9601 acaataattt gactagaagt tgattcgggt gtttccggaa ggggccgagt caatccgccg
     9661 agttggggca cggaaaacaa aaagggaagg ctactaagat ttttctggcg ggggttatca
     9721 ttggcgtaac tgcagggacc acctcccggc ggccgcctaa tggatcctag tactcgagaa
     9781 aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tatttaaact
     9841 tgctatgctg tttccagctt agctcttaaa cacttcacac gctgaccttc ccatgtttct
     9901 ggctttccac aagatatata aagccaagaa atcgaaatac tttcaagtta cggtaagcat
     9961 atgatagtcc attttaaaac ataattttaa aactgcaaac tacccaagaa attattactt
    10021 tctacgtcac gtattttgta ctaatatctt tgtgtttaca gtcaaattaa ttccaattat
    10081 ctctctaaca gccttgtatc gtatatgcaa atatgaagga atcatgggaa ataggccctc
    10141 ttcctgcccg accttggg
//
