LOCUS       sequence-484681-        9565 bp    DNA     circular SYN 26-AUG-2025
DEFINITION  Dual sgRNA empty vector expressing BFP with Puromycin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9565)
  AUTHORS   Thayer JA, Petersen JD, Huang X, Hawrot J, Ramos DM, Sekine S, Li Y,
            Ward ME, Narendra DP
  TITLE     Novel reporter of the PINK1-Parkin mitophagy pathway identifies its 
            damage sensor in the import gate.
  JOURNAL   bioRxiv [Preprint]. 2025 Feb 20:2025.02.19.639160. doi: 
            10.1101/2025.02.19.639160.
  PUBMED    40027798
REFERENCE   2  (bases 1 to 9565)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 26, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2025 Feb 20:2025.02.19.639160. doi: 
            10.1101/2025.02.19.639160."
COMMENT     Sequence Label: pLenti_DualsgRNAEmptyVector_Puro_T2A_BFP2
FEATURES             Location/Qualifiers
     source          1..9565
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    221..338
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(636..680)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    865..1098
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1595..1720
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1767..1947
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(1965..2163)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1990..2010)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2165..2544
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     2716..2735
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        2810..2914
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2915..2983,2984..3775)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3133..3152)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3946..4534
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4435..4454
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             4596..4776
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     5285..5301
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     5286..5302
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    5305..5893
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5411..5422
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5820..5840
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    5949..5982
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(6002..6700)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     CDS             complement(6713..6766)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(6776..7372)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6856..6876)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     7353..7372
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(7384..8562)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          7393..8331
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(7416..7436)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     complement(9476..9496)
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     protein_bind    9500..9533
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 agtattaaaa agaacagatt atctgtcttt tatcgcacat taagcctcta tagttactag
       61 gaaatattat atgcaaatta accggggcag gggagtagcc gagcttctcc cacaagtccg
      121 tgcgaggggg ccggcgcggg cctagagatg gcgcgtcgga tctctagagc gggcccggta
      181 ctaaccaaac tggatctctg ctgtccctgt aataaacccg aaaattttga atttttgtaa
      241 tttgtttttg taattcttta gtttgtatgt ctgttgctat tatgtctact attctttccc
      301 ctgcactgta ccccccaatc cccccttttc ttttaaaatt gtggatgaat actgccattt
      361 gtctgcagaa ttggcgcacg cagtgccgat ccgttcacta atcgaatgga tctgtctctg
      421 tctctctctc caccttcttc ttctattcct tcgggcctgt cgggtcccct cggggttggg
      481 aggtgggtct gaaacgataa tggtgaatat ccctgcctaa ctctattcac tatagaaagt
      541 acagcaaaaa ctattcttaa acctaccaag cctcctacta tcattatgaa taattttata
      601 taccacagcc aatttgttat gttaaaccaa ttccacaaac ttgcccattt atctaattcc
      661 aataattctt gttcattctt ttcttgctgg ttttgcgatt cttcaattaa ggagtgtatt
      721 aagcttgtgt aattgttaat ttctctgtcc cactccatcc aggtcgtgtg attccaaatc
      781 tgttccagag atttattact ccaactagca ttccaaggca cagcagtggt gcaaatgagt
      841 tttccagagc aaccccaaat ccccaggagc tgttgatcct ttaggtatct ttccacagcc
      901 aggattcttg cctggagctg cttgatgccc cagactgtga gttgcaacag atgctgttgc
      961 gcctcaatag ccctcagcaa attgttctgc tgctgcacta taccagacaa taattgtctg
     1021 gcctgtaccg tcagcgtcat tgacgctgcg cccatagtgc ttcctgctgc tcccaagaac
     1081 ccaaggaaca aagctcctat tcccactgct cttttttctc tctgcaccac tcttctcttt
     1141 gccttggtgg gtgctactcc taatggttca atttttacta ctttatattt atataattca
     1201 cttctccaat tgtccctcat atctcctcct ccaggtctga agatcagcgg ccggccgctt
     1261 gctgtgcggt ggtcttactt ttgttttgct cttcctctat cttgtctaaa gcttccttgg
     1321 tgtcttttat ctctatcctt tgatgcacac aatagagggt tgctactgta ttatataatg
     1381 atctaagttc ttctgatcct gtctgaaggg atggttgtag ctgtcccagt atttgtctac
     1441 agccttctga tgtttctaac aggccaggat taactgcgaa tcgttctagc tccctgcttg
     1501 cccatactat atgttttaat ttatattttt tctttccccc tggccttaac cgaatttttt
     1561 cccatcgcga tctaattctc ccccgcttaa tactgacgct ctcgcaccca tctctctcct
     1621 tctagcctcc gctagtcaaa atttttggcg tactcaccag tcgccgcccc tcgcctcttg
     1681 ccgtgcgcgc ttcagcaagc cgagtcctgc gtcgagagag ctcctctggt ttccctttcg
     1741 ctttcaagtc cctgttcggg cgccactgct agagattttc cacactgact aaaagggtct
     1801 gagggatctc tagttaccag agtcacacaa cagacgggca cacactactt gaagcactca
     1861 aggcaagctt tattgaggct taagcagtgg gttccctagt tagccagaga gctcccaggc
     1921 tcagatctgg tctaaccaga gagacccagt acaggcaaaa cgcgctgctt atatagacct
     1981 cccaccgtac acgcctaccg cccatttgcg tcaatggggc ggagttgtta cgacattttg
     2041 gaaagtcccg ttgattttgg tgccaaaaca aactcccatt gacgtcaatg gggtggagac
     2101 ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt gatgtactgc caaaaccgca
     2161 tcaccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga aagtcccata
     2221 aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg tcaatagggg
     2281 gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac cgtaaatact
     2341 ccacccattg acgtcaatgg aaagtcccta ttggcgttac tatgggaaca tacgtcatta
     2401 ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt accgtaagtt
     2461 atgtaacgcg gaactccata tatgggctat gaactaatga ccccgtaatt gattactatt
     2521 aataactagt caataatcaa tgtcaacgcg tatatctggc ccgtacatcg cgaagcagcg
     2581 caaaacgcct aaccctaagc agattcttca tgcaattgtc ggtcaagcct tgccttgttg
     2641 tagcttaaat tttgctcgcg cactactcag cgacctccaa cacacaagca gggagcagat
     2701 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat aggggatcgg
     2761 gagatctccc gatccgtcga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     2821 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     2881 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     2941 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     3001 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     3061 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     3121 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     3181 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     3241 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     3301 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     3361 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     3421 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     3481 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     3541 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     3601 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     3661 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     3721 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     3781 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     3841 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     3901 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     3961 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     4021 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     4081 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     4141 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     4201 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     4261 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     4321 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     4381 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     4441 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     4501 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     4561 gttcctggcc ttttgctggc cttttgctca catgctgcta gagattttcc acactgacta
     4621 aaagggtctg agggatctct agttaccaga gtcacacaac agacgggcac acactacttg
     4681 aagcactcaa ggcaagcttt attgaggctt aagcagtggg ttccctagtt agccagagag
     4741 ctcccaggct cagatctggt ctaaccagag agacccagta cagtccggat gcagctctcg
     4801 ggccatgtga tgaaatgcta ggcggctgtc aaacctccac tctaatactt ctctctccgg
     4861 gtcatccatc ccatgcaggc tcacagggtg taacaagcgg gtgttctctc cttcattggc
     4921 ttcttctacc ttctcttgct caactggtac tagcttgtag caccatccaa aggtcagtgg
     4981 atatctgatc cctggccctg gtgtgtagtt ctgccaatca gggaagtagc cttgtgtgtg
     5041 gtagatccac agatcaagga tatcttgtct tcgttgggag tgaattagcc cttccagtcc
     5101 ccccttttct tttaaaaagt ggctaagatc tacagctgcc ttgtaagtca ttggtcttaa
     5161 aggtacctga ggtgtgactg gaaaacccac ctcctcctcc tcttgtgctt ctagccaggc
     5221 acaatcagca ttggtagctg ctgtattgct acttgtgatt gctccatgtt tttctaggtc
     5281 tcgatcgagg tcgacggtat cgatgcgggg aggcggccca aagggagatc cgactcgtct
     5341 gagggcgaag gcgaagacgc ggaagaggcc gcagagccgg cagcaggccg cgggaaggaa
     5401 ggtccgctgg attgagggcc gaagggacgt agcagaagga cgtcccgcgc agaatccagg
     5461 tggcaacaca ggcgagcagc caaggaaagg acgatgattt ccccgacaac accacggaat
     5521 tgtcagtgcc caacagccga gcccctgtcc agcagcgggc aaggcaggcg gcgatgagtt
     5581 ccgccgtggc aatagggagg gggaaagcga aagtcccgga aaggagctga caggtggtgg
     5641 caatgcccca accagtgggg gttgcgtcag caaacacagt gcacaccacg ccacgttgcc
     5701 tgacaacggg ccacaactcc tcataaagag acagcaacca ggatttatac aaggaggaga
     5761 aaatgaaagc catacgggaa gcaatagcat gatacaaagg cattaaagca gcgtatccac
     5821 atagcgtaaa aggagcaaca tagttaagaa taccagtcaa tctttcacaa attttgtaat
     5881 ccagaggttg attatcgata agcttgatat cgaattgtac ctagtggaac cggaaccctt
     5941 aaacatgtat aacttcgtat aatgtatgct atacgaagtt attaggtccc tcgacgaatt
     6001 cttaattaag cttgtgcccc agtttgctag ggaggtcgca gtatctggcc actgccacct
     6061 cgtgctgctc gacgtaggtc tcgttgttgg cctccttgat tctttccagt ctgtagtcca
     6121 catagtagac gccaggcatc ttgaggttct tagcgggttt cttggatcta tatgtggtct
     6181 tgatgtttgc gatcagatgg ctcccgccca cgagcttcag ggccatgtcg tttctgcctt
     6241 ccaggccgcc gtcagcgggg tacagggtct cggtgaaggc ctcccagccg agtgttttct
     6301 tctgcatcac agggccgttg gatgtgaagt tcacccctct gatcttgacg ttgtagatga
     6361 ggcagccgtc ctggaggctg gtgtcctggg tagcggtcag cacgcccccg tcttcgtatg
     6421 tggtgactct ctcccatgtg aagccctcag ggaaggactg cttgaagaag tcggggatgc
     6481 cctgggtgtg gttgatgaag gtcttgctgc cgtagaggaa gctagtagcc aggatgtcga
     6541 aggcgaaggg gagagggccg ccctcgacca ccttgattct catggtctgg gtgccctcgt
     6601 agggcttgcc ttcgccctcg gatgtgcact tgaagtgatg gttgtccacg gtgccctcca
     6661 tgtacagctt catgtgcatg ttctccttaa tcagctcgct ggtggcgacc ggtgggccag
     6721 gattctcctc gacgtcaccg catgttagca gacttcctct gccctctccc gatccggcac
     6781 cgggcttgcg ggtcatgcac caggtgcgcg gtccttcggg cacctcgacg tcggcggtga
     6841 cggtgaagcc gagccgctcg tagaagggga ggttgcgggg cgcggaggtc tccaggaagg
     6901 cgggcacccc ggcgcgctcg gccgcctcca ctccggggag cacgacggcg ctgcccagac
     6961 ccttgccctg gtggtcgggc gacacgccga cggtggccag gaaccacgcg ggctccttgg
     7021 gccggtgcgg cgccaggagg ccttccatct gttgctgcgc ggccagccgg gaaccgctca
     7081 actcggccat gcgcgggccg atctcggcga acaccgcccc cgcttcgacg ctctccggcg
     7141 tggtccagac cgccaccgcg gcgccgtcgt ccgcgaccca caccttgccg atgtcgagcc
     7201 cgacgcgcgt gaggaagagt tcttgcagct cggtgacccg ctcgatgtgg cggtccgggt
     7261 cgacggtgtg gcgcgtggcg gggtagtcgg cgaacgcggc ggcgagggtg cgtacggccc
     7321 gggggacgtc gtcgcgggtg gcgaggcgca ccgtgggctt gtactcggtc atggtgggct
     7381 agctcacgac acctgaaatg gaagaaaaaa actttgaacc actgtctgag gcttgagaat
     7441 gaaccaagat ccaaactcaa aaagggcaaa ttccaaggag aattacatca agtgccaagc
     7501 tggcctaact tcagtctcca cccactcagt gtggggaaac tccatcgcat aaaacccctc
     7561 cccccaacct aaagacgacg tactccaaaa gcgcgagaac taatcgaggt gcctggacgg
     7621 cgcccggtac tccgtggagt cacatgaagc gacggctgag gacggaaagg cccttttcct
     7681 ttgtgtgggt gactcacccg cccgctctcc cgagcgccgc gtcctccatt ttgagctccc
     7741 tgcagcaggg ccgggaagcg gccatctttc cgctcacgca actggtgccg accgggccag
     7801 ccttgccgcc cagggcgggg cgatacacgg cggcgcgagg ccaggcacca gagcaggccg
     7861 gccagcttga gactaccccc gtccgattct cggtggccgc gctcgcaggc cccgcctcgc
     7921 cgaacatgtg cgctgggacg cacgggcccc gtcgccgccc gcggccccaa aaaccgaaat
     7981 accagtgtgc agatcttggc ccgcatttac aagactatct tgccagaaaa aaagcgtcgc
     8041 agcaggtcat caaaaatttt aaatggctag agacttatcg aaagcagcga gacaggcgcg
     8101 aaggtgccac cagattcgca cgcggcggcc ccagcgccca ggccaggcct caactcaagc
     8161 acgaggcgaa ggggctcctt aagcgcaagg cctcgaactc tcccacccac ttccaacccg
     8221 aagctcggga tcaagaatca cgtactgcag ccaggtggaa gtaattcaag gcacgcaagg
     8281 gccataaccc gtaaagaggc caggcccgcg ggaaccacac acggcactta cctgtgttct
     8341 ggcggcaaac ccgttgcgaa aaagaacgtt cacggcgact actgcactta tatacggttc
     8401 tcccccaccc tcgggaaaaa ggcggagcca gtacacgaca tcactttccc agtttacccc
     8461 gcgccacctt ctctaggcac cggttcaatt gccgacccct ccccccaact tctcggggac
     8521 tgtgggcgat gtgcgctctg cccactgacg ggcacatgca tctccgcgcc tacagctcaa
     8581 gccacatccg aagggggagg gagccgggag ctgcgcgcgg ggccgccggg gggaggggtg
     8641 gcaccgccca cgccgggcgg ccacgaaggg cggggcagcg ggcgcgcgcc cggcgggggg
     8701 aggggccgcg cgccgcgccc gctgggaatt ggggccctag ggggagggcg gaggcgccga
     8761 cgaccgcggc acttaccgtt cgcggcgtgg cgcccggtgg tccccaaggg gagggaaggg
     8821 ggaggcgggg cgaggacagt gaccggagtc tcctcagcgg tggcttttct gcttggcagc
     8881 ctcagcggct ggcgccaaaa ccggactccg cccacttcct cgcccctgcg gtgcgagggt
     8941 gtggaatcct ccagacgctg ggggaggggg agttgggagc ttaaaaacta gtaccccttt
     9001 gggaccactt tcagcagcga actctcctgt acaccagggg tcagttccac agacgcgggc
     9061 caggggtggg tcattgcggc gtgaacaata atttgactag aagttgattc gggtgtttcc
     9121 ggaaggggcc gagtcaatcc gccgagttgg ggcacggaaa acaaaaaggg aaggctacta
     9181 agatttttct ggcgggggtt atcattggcg taactgcagg gaccacctcc cggcggccgc
     9241 ctaatggatc ctagtactcg agaaaaaaag caccgactcg gtgccacttt ttcaagttga
     9301 taacggacta gccttattta aacttgctat gctgtttcca gcttagctct taaacgagac
     9361 gatggtcgtc ttcacaagtt atcagatctg gaaagcgaag acgactcccg tctcaacaag
     9421 gtggttctcc aagggatact tatagtctca aaacacacaa ttactttaca gttagggtga
     9481 gtttcctttt gtgctgttta taacttcgta taatgtatgc tatacgaagt tatagaaatc
     9541 caagcctatc atgtaaaatg tagct
//
